Rroxscaffold_6G00394800

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002743
Physical Location & Seq
Reverse (-)
15672873 .. 15676441
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394800.1

Sequence Viewer

Length: 816 bp
ATGAAGGCATTGGCTGTTGTTTGCCCCCCAAGAATTTCCTTCTCCTGCTCCTCCTCATGGCAACAACGACACCTTCGGTTTCGGTCCAAACCGAAATCTCAGCTCCGAATTAGCTCACGGTTTCGGGTCTCGTCTTGTGTGATAAAAGAGGGAGAAAGCGAATCAGAAAGGCAGAAATACAAAGGTGGAGTGGTGGATGAGTTGTTGCTGAATGTGTTCAGGAACAAAATGGTTAAACAAGTGGGATGGGATTCCCATAAGCCGGGATACGATGGACTAATTGAAGTTGCCAACCGGATGATGCTTAACAACCCAACAAATTCTGATACTAAAGAAGCTGCGGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTATAAACTGCTTATAGCTCCTATACAAGGCGGAGAAATAGCCGCCCTCATGGTTGCAAGGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCCTGCACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGGGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGCTGCCAGTTTAAATTCGGGGTCCTTCCTCCACTTTCCGAAGACGACGCCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTACCCGAAGAAGAGAAAAAAACATTAATATGGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

271

Amino Acids

30.43

Weight (kDa)

8.84

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 157 - 238 3.3e-35 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 728
AccI GTMKAC 1 cut(s) 501
AciI CCGC 4 cut(s) 341, 417, 429, 726
AclWI GGATC 1 cut(s) 590
AcsI RAATTY 4 cut(s) 33, 319, 517, 689
AcyI GRCGYC 1 cut(s) 723
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 3 cut(s) 57, 262, 413
AgsI TTSAA 3 cut(s) 284, 625, 667
AhlI ACTAGT 1 cut(s) 527
AluBI AGCT 6 cut(s) 103, 114, 338, 404, 604, 678
AluI AGCT 6 cut(s) 103, 114, 338, 404, 604, 678
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 1 cut(s) 590
AoxI GGCC 1 cut(s) 478
ApaI GGGCCC 1 cut(s) 482
ApeKI GCWGC 2 cut(s) 338, 678
ApoI RAATTY 4 cut(s) 33, 319, 517, 689
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 215
AspS9I GGNCC 5 cut(s) 84, 343, 478, 479, 697
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 1 cut(s) 460
AvaII GGWCC 3 cut(s) 84, 343, 697
BaeGI GKGCMC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 325, 665
BccI CCATC 4 cut(s) 240, 266, 506, 646
BciVI GTATCC 1 cut(s) 260
BcnI CCSGG 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 1 cut(s) 673
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 4 cut(s) 339, 429, 679, 726
BlpI GCTNAGC 1 cut(s) 745
BlsI GCNGC 4 cut(s) 340, 430, 680, 727
BmcAI AGTACT 1 cut(s) 557
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 3 cut(s) 84, 343, 697
BmgT120I GGNCC 5 cut(s) 84, 343, 478, 479, 697
BmiI GGNNCC 5 cut(s) 480, 481, 657, 698, 738
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 723
Bpu1102I GCTNAGC 1 cut(s) 745
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 807
BsaWI WCCGGW 1 cut(s) 294
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 262, 413
Bse1I ACTGG 1 cut(s) 682
BseDI CCNNGG 1 cut(s) 807
BseGI GGATG 3 cut(s) 202, 251, 303
BseLI CCNNNNNNNGG 3 cut(s) 57, 262, 413
BseMII CTCAG 2 cut(s) 113, 626
BseNI ACTGG 1 cut(s) 682
BseRI GAGGAG 2 cut(s) 40, 43
BseSI GKGCMC 1 cut(s) 482
BseXI GCAGC 2 cut(s) 325, 665
BsgI GTGCAG 1 cut(s) 469
BshFI GGCC 1 cut(s) 480
BsiSI CCGG 2 cut(s) 263, 295
BslFI GGGAC 2 cut(s) 626, 789
BslI CCNNNNNNNGG 3 cut(s) 57, 262, 413
BsmAI GTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 626, 789
BsnI GGCC 1 cut(s) 480
Bso31I GGTCTC 1 cut(s) 133
Bsp120I GGGCCC 1 cut(s) 478
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 1 cut(s) 582
Bsp1720I GCTNAGC 1 cut(s) 745
BspACI CCGC 4 cut(s) 341, 417, 429, 726
BspANI GGCC 1 cut(s) 480
BspCNI CTCAG 2 cut(s) 112, 625
BspLI GGNNCC 5 cut(s) 480, 481, 657, 698, 738
BspPI GGATC 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 133
BsrBI CCGCTC 1 cut(s) 728
BsrI ACTGG 1 cut(s) 682
BssECI CCNNGG 1 cut(s) 807
BssMI GATC 1 cut(s) 582
BssNI GRCGYC 1 cut(s) 723
BssT1I CCWWGG 1 cut(s) 807
Bst4CI ACNGT 3 cut(s) 120, 387, 490
Bst6I CTCTTC 1 cut(s) 769
BstACI GRCGYC 1 cut(s) 723
BstDEI CTNAG 3 cut(s) 99, 612, 745
BstENI CCTNNNNNAGG 1 cut(s) 411
BstF5I GGATG 3 cut(s) 202, 251, 303
BstKTI GATC 1 cut(s) 585
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 262
BstSFI CTRYAG 1 cut(s) 673
BstSLI GKGCMC 1 cut(s) 482
BstV1I GCAGC 2 cut(s) 325, 665
BstV2I GAAGAC 1 cut(s) 723
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 1 cut(s) 480
BtsCI GGATG 3 cut(s) 202, 251, 303
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 2 cut(s) 486, 806
Cfr13I GGNCC 5 cut(s) 84, 343, 478, 479, 697
CpoI CGGWCCG 1 cut(s) 343
CseI GACGC 1 cut(s) 731
Csp6I GTAC 1 cut(s) 556
CspI CGGWCCG 1 cut(s) 343
CviAII CATG 5 cut(s) 57, 436, 475, 522, 634
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 3 cut(s) 99, 612, 745
DpnI GATC 1 cut(s) 584
DpnII GATC 1 cut(s) 582
DraI TTTAAA 1 cut(s) 688
Eam1104I CTCTTC 1 cut(s) 769
EarI CTCTTC 1 cut(s) 769
EciI GGCGGA 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 357
Eco47I GGWCC 3 cut(s) 84, 343, 697
EcoNI CCTNNNNNAGG 1 cut(s) 411
EcoO109I RGGNCCY 2 cut(s) 479, 697
EcoRI GAATTC 1 cut(s) 517
EcoRV GATATC 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 807
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 807
FaeI CATG 5 cut(s) 60, 439, 478, 525, 637
FaqI GGGAC 2 cut(s) 626, 789
FatI CATG 5 cut(s) 56, 435, 474, 521, 633
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 4 cut(s) 339, 429, 679, 726
FokI GGATG 3 cut(s) 209, 258, 310
FriOI GRGCYC 1 cut(s) 482
Fsp4HI GCNGC 4 cut(s) 339, 429, 679, 726
FspBI CTAG 1 cut(s) 528
GluI GCNGC 4 cut(s) 339, 429, 679, 726
HaeIII GGCC 1 cut(s) 480
HapII CCGG 2 cut(s) 263, 295
HgaI GACGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 723
Hin1II CATG 5 cut(s) 60, 439, 478, 525, 637
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 2 cut(s) 161, 251
HpaII CCGG 2 cut(s) 263, 295
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 1 cut(s) 502
Hpy188I TCNGA 6 cut(s) 107, 166, 325, 347, 354, 715
Hpy188III TCNNGA 2 cut(s) 220, 544
Hpy8I GTNNAC 1 cut(s) 502
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 3 cut(s) 49, 83, 710
HpyCH4III ACNGT 3 cut(s) 120, 387, 490
HpyCH4V TGCA 4 cut(s) 443, 455, 486, 552
HpyF3I CTNAG 3 cut(s) 99, 612, 745
Hsp92I GRCGYC 1 cut(s) 723
Hsp92II CATG 5 cut(s) 60, 439, 478, 525, 637
Kzo9I GATC 1 cut(s) 582
LmnI GCTCC 5 cut(s) 53, 108, 409, 736, 796
LpnPI CCDG 7 cut(s) 58, 205, 276, 308, 389, 496, 695
Lsp1109I GCAGC 2 cut(s) 325, 665
LweI GCATC 1 cut(s) 291
MaeI CTAG 1 cut(s) 528
MaeIII GTNAC 1 cut(s) 448
MalI GATC 1 cut(s) 584
MbiI CCGCTC 1 cut(s) 728
MboI GATC 1 cut(s) 582
MboII GAAGA 3 cut(s) 728, 783, 786
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 7 cut(s) 33, 108, 279, 319, 493, 517, 689
MmeI TCCRAC 1 cut(s) 632
MnlI CCTC 6 cut(s) 61, 64, 142, 443, 556, 714
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 5 cut(s) 234, 306, 492, 687, 789
MslI CAYNNNNRTG 1 cut(s) 791
MspI CCGG 2 cut(s) 263, 295
MspR9I CCNGG 1 cut(s) 264
NciI CCSGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 752
NdeII GATC 1 cut(s) 582
NlaIII CATG 5 cut(s) 60, 439, 478, 525, 637
NlaIV GGNNCC 5 cut(s) 480, 481, 657, 698, 738
NmuCI GTSAC 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 161, 251
PkrI GCNGC 4 cut(s) 340, 430, 680, 727
PpuMI RGGWCCY 1 cut(s) 697
PshBI ATTAAT 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 697
PspN4I GGNNCC 5 cut(s) 480, 481, 657, 698, 738
PspOMI GGGCCC 1 cut(s) 478
PspPI GGNCC 5 cut(s) 84, 343, 478, 479, 697
PspPPI RGGWCCY 1 cut(s) 697
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
RseI CAYNNNNRTG 1 cut(s) 791
Rsr2I CGGWCCG 1 cut(s) 343
RsrII CGGWCCG 1 cut(s) 343
SalI GTCGAC 1 cut(s) 500
SaqAI TTAA 5 cut(s) 234, 306, 492, 687, 789
SatI GCNGC 4 cut(s) 339, 429, 679, 726
Sau3AI GATC 1 cut(s) 582
Sau96I GGNCC 5 cut(s) 84, 343, 478, 479, 697
ScaI AGTACT 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 1 cut(s) 482
SetI ASST 9 cut(s) 75, 105, 116, 187, 340, 406, 507, 606, 680
SfaNI GCATC 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 673
SinI GGWCC 3 cut(s) 84, 343, 697
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 7 cut(s) 33, 108, 279, 319, 493, 517, 689
SsiI CCGC 4 cut(s) 341, 417, 429, 726
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 262
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 3 cut(s) 120, 387, 490
TaqI TCGA 2 cut(s) 501, 543
TaqII GACCGA 1 cut(s) 72
TasI AATT 7 cut(s) 33, 108, 279, 319, 493, 517, 689
TatI WGTACW 1 cut(s) 555
TauI GCSGC 2 cut(s) 431, 728
TfiI GAWTC 2 cut(s) 161, 251
Tru1I TTAA 5 cut(s) 234, 306, 492, 687, 789
Tru9I TTAA 5 cut(s) 234, 306, 492, 687, 789
TscAI CASTG 2 cut(s) 493, 806
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 2 cut(s) 338, 678
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 2 cut(s) 17, 510
TspRI CASTG 2 cut(s) 493, 806
VpaK11BI GGWCC 3 cut(s) 84, 343, 697
VspI ATTAAT 1 cut(s) 789
XagI CCTNNNNNAGG 1 cut(s) 411
XapI RAATTY 4 cut(s) 33, 319, 517, 689
XmiI GTMKAC 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 528
ZrmI AGTACT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.