Rh3DG313500

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002743
Physical Location & Seq
Forward (+)
33668332 .. 33670783
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG313500.1

Sequence Viewer

Length: 807 bp
ATGAAGGCATTGGCTGTTGTTTGCCCCCCAAGAATTTCCTTCTCCTGCTCCTCCTCCTCATGGCAACAACGACACCTTCGGCTTCGGTCCAAACCGAAATCCCAGCTCCGAATTAGCTCACGGTTTCGGGTCTCGTCTTGTTTGATAAAAGAGGGAGAAGGGCAGAAACACAAAGGTGGAGTGGTGGATGAGTTGTTGCTGAATGTGTTCAGGAACAAAATGGTTAAACAAGTGGGATGGGATTCCGATAAGCCGGGATACGATGGACTAATTGAAGTGGCCAACCGGATGATGCTTAACAACCCAACAAATTCTGATACTAAAGAAGCTGCCGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTATAAACTGCTTATATCTCCTATACAAGGCGGAAAAATAGCTGCCCTCATGGTTGGTCAGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCCTGTACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGGGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGCTGCCAGTTTAAATTCGGGGTCCTTCCTCCACTTCCCGAAGACGACGCCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTACCCGAAGAAGAGAAAGAAACATTAATATAGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

268

Amino Acids

29.91

Weight (kDa)

8.94

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 154 - 235 3.4e-35 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 719
AccI GTMKAC 1 cut(s) 492
AciI CCGC 2 cut(s) 408, 717
AclWI GGATC 1 cut(s) 581
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 4 cut(s) 33, 310, 508, 680
AcyI GRCGYC 1 cut(s) 714
AfaI GTAC 2 cut(s) 478, 548
AfiI CCNNNNNNNGG 2 cut(s) 60, 404
AgsI TTSAA 3 cut(s) 275, 616, 658
AhlI ACTAGT 1 cut(s) 518
AleI CACNNNNGTG 1 cut(s) 174
AluBI AGCT 6 cut(s) 106, 117, 329, 419, 595, 669
AluI AGCT 6 cut(s) 106, 117, 329, 419, 595, 669
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 1 cut(s) 581
AoxI GGCC 2 cut(s) 279, 469
ApaI GGGCCC 1 cut(s) 473
ApeKI GCWGC 3 cut(s) 329, 419, 669
ApoI RAATTY 4 cut(s) 33, 310, 508, 680
AseI ATTAAT 1 cut(s) 780
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 4 cut(s) 87, 469, 470, 688
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 451
AvaII GGWCC 2 cut(s) 87, 688
BaeGI GKGCMC 1 cut(s) 473
BalI TGGCCA 1 cut(s) 281
BanII GRGCYC 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 714
BbvI GCAGC 3 cut(s) 316, 406, 656
BccI CCATC 4 cut(s) 231, 257, 497, 637
BceAI ACGGC 1 cut(s) 317
BciVI GTATCC 1 cut(s) 251
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 136
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 664
BfuI GTATCC 1 cut(s) 251
BisI GCNGC 4 cut(s) 330, 420, 670, 717
BlpI GCTNAGC 1 cut(s) 736
BlsI GCNGC 4 cut(s) 331, 421, 671, 718
BmcAI AGTACT 1 cut(s) 548
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 2 cut(s) 87, 688
BmgT120I GGNCC 4 cut(s) 87, 469, 470, 688
BmiI GGNNCC 5 cut(s) 471, 472, 648, 689, 729
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 714
Bpu1102I GCTNAGC 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 798
BsaWI WCCGGW 1 cut(s) 285
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 404
Bse1I ACTGG 1 cut(s) 673
BseDI CCNNGG 1 cut(s) 798
BseGI GGATG 3 cut(s) 193, 242, 294
BseLI CCNNNNNNNGG 2 cut(s) 60, 404
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 3 cut(s) 40, 43, 46
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 3 cut(s) 316, 406, 656
BseYI CCCAGC 1 cut(s) 102
BshFI GGCC 2 cut(s) 281, 471
BsiSI CCGG 2 cut(s) 254, 286
BslFI GGGAC 2 cut(s) 617, 780
BslI CCNNNNNNNGG 2 cut(s) 60, 404
BsmAI GTCTC 1 cut(s) 136
BsmFI GGGAC 2 cut(s) 617, 780
BsnI GGCC 2 cut(s) 281, 471
Bso31I GGTCTC 1 cut(s) 136
Bsp120I GGGCCC 1 cut(s) 469
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 1 cut(s) 573
Bsp1720I GCTNAGC 1 cut(s) 736
BspACI CCGC 2 cut(s) 408, 717
BspANI GGCC 2 cut(s) 281, 471
BspCNI CTCAG 1 cut(s) 616
BspLI GGNNCC 5 cut(s) 471, 472, 648, 689, 729
BspPI GGATC 1 cut(s) 581
BspTNI GGTCTC 1 cut(s) 136
BsrBI CCGCTC 1 cut(s) 719
BsrI ACTGG 1 cut(s) 673
BssECI CCNNGG 1 cut(s) 798
BssMI GATC 1 cut(s) 573
BssNI GRCGYC 1 cut(s) 714
BssT1I CCWWGG 1 cut(s) 798
Bst4CI ACNGT 3 cut(s) 123, 378, 481
Bst6I CTCTTC 1 cut(s) 760
BstACI GRCGYC 1 cut(s) 714
BstDEI CTNAG 2 cut(s) 603, 736
BstENI CCTNNNNNAGG 1 cut(s) 402
BstF5I GGATG 3 cut(s) 193, 242, 294
BstKTI GATC 1 cut(s) 576
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 1 cut(s) 573
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 664
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 3 cut(s) 316, 406, 656
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 1 cut(s) 573
BstYI RGATCY 1 cut(s) 573
BsuI GTATCC 1 cut(s) 251
BsuRI GGCC 2 cut(s) 281, 471
BtsCI GGATG 3 cut(s) 193, 242, 294
BtsI GCAGTG 1 cut(s) 797
BtsIMutI CAGTG 1 cut(s) 797
Cfr13I GGNCC 4 cut(s) 87, 469, 470, 688
CseI GACGC 1 cut(s) 722
Csp6I GTAC 2 cut(s) 477, 547
CviAII CATG 5 cut(s) 60, 427, 466, 513, 625
CviQI GTAC 2 cut(s) 477, 547
DdeI CTNAG 2 cut(s) 603, 736
DpnI GATC 1 cut(s) 575
DpnII GATC 1 cut(s) 573
DraI TTTAAA 1 cut(s) 679
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 760
EarI CTCTTC 1 cut(s) 760
EciI GGCGGA 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 798
Eco24I GRGCYC 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 136
Eco32I GATATC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 87, 688
EcoNI CCTNNNNNAGG 1 cut(s) 402
EcoO109I RGGNCCY 2 cut(s) 470, 688
EcoRI GAATTC 1 cut(s) 508
EcoRV GATATC 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 798
EcoT38I GRGCYC 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 798
FaeI CATG 5 cut(s) 63, 430, 469, 516, 628
FaqI GGGAC 2 cut(s) 617, 780
FatI CATG 5 cut(s) 59, 426, 465, 512, 624
FauNDI CATATG 1 cut(s) 743
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 330, 420, 670, 717
FokI GGATG 3 cut(s) 200, 249, 301
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 4 cut(s) 330, 420, 670, 717
FspBI CTAG 1 cut(s) 519
GluI GCNGC 4 cut(s) 330, 420, 670, 717
GsaI CCCAGC 1 cut(s) 106
HaeIII GGCC 2 cut(s) 281, 471
HapII CCGG 2 cut(s) 254, 286
HgaI GACGC 1 cut(s) 722
Hin1I GRCGYC 1 cut(s) 714
Hin1II CATG 5 cut(s) 63, 430, 469, 516, 628
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 1 cut(s) 242
HpaII CCGG 2 cut(s) 254, 286
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 5 cut(s) 110, 247, 316, 338, 345
Hpy188III TCNNGA 3 cut(s) 211, 535, 704
Hpy8I GTNNAC 1 cut(s) 493
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 4 cut(s) 49, 86, 152, 701
HpyCH4III ACNGT 3 cut(s) 123, 378, 481
HpyCH4V TGCA 2 cut(s) 446, 543
HpyF3I CTNAG 2 cut(s) 603, 736
Hsp92I GRCGYC 1 cut(s) 714
Hsp92II CATG 5 cut(s) 63, 430, 469, 516, 628
Kzo9I GATC 1 cut(s) 573
LmnI GCTCC 3 cut(s) 53, 111, 727
LpnPI CCDG 9 cut(s) 58, 116, 196, 267, 299, 380, 422, 487, 686
Lsp1109I GCAGC 3 cut(s) 316, 406, 656
LweI GCATC 1 cut(s) 282
MaeI CTAG 1 cut(s) 519
MaeIII GTNAC 1 cut(s) 439
MalI GATC 1 cut(s) 575
MbiI CCGCTC 1 cut(s) 719
MboI GATC 1 cut(s) 573
MboII GAAGA 3 cut(s) 719, 774, 777
MflI RGATCY 1 cut(s) 573
MhlI GDGCHC 1 cut(s) 473
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 7 cut(s) 33, 111, 270, 310, 484, 508, 680
MluNI TGGCCA 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 623
MnlI CCTC 7 cut(s) 61, 64, 67, 145, 434, 547, 705
Mox20I TGGCCA 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 281
MseI TTAA 5 cut(s) 225, 297, 483, 678, 780
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 2 cut(s) 254, 286
MspR9I CCNGG 1 cut(s) 255
NciI CCSGG 1 cut(s) 255
NdeI CATATG 1 cut(s) 743
NdeII GATC 1 cut(s) 573
NlaIII CATG 5 cut(s) 63, 430, 469, 516, 628
NlaIV GGNNCC 5 cut(s) 471, 472, 648, 689, 729
NmuCI GTSAC 1 cut(s) 439
OliI CACNNNNGTG 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 4 cut(s) 331, 421, 671, 718
PpuMI RGGWCCY 1 cut(s) 688
PshBI ATTAAT 1 cut(s) 780
Psp5II RGGWCCY 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 102
PspN4I GGNNCC 5 cut(s) 471, 472, 648, 689, 729
PspOMI GGGCCC 1 cut(s) 469
PspPI GGNCC 4 cut(s) 87, 469, 470, 688
PspPPI RGGWCCY 1 cut(s) 688
PsuI RGATCY 1 cut(s) 573
RsaI GTAC 2 cut(s) 478, 548
RsaNI GTAC 2 cut(s) 477, 547
RseI CAYNNNNRTG 1 cut(s) 174
SalI GTCGAC 1 cut(s) 491
SaqAI TTAA 5 cut(s) 225, 297, 483, 678, 780
SatI GCNGC 4 cut(s) 330, 420, 670, 717
Sau3AI GATC 1 cut(s) 573
Sau96I GGNCC 4 cut(s) 87, 469, 470, 688
ScaI AGTACT 1 cut(s) 548
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 664
SinI GGWCC 2 cut(s) 87, 688
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 518
Sse9I AATT 7 cut(s) 33, 111, 270, 310, 484, 508, 680
SsiI CCGC 2 cut(s) 408, 717
SspMI CTAG 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 1 cut(s) 798
TaaI ACNGT 3 cut(s) 123, 378, 481
TaqI TCGA 2 cut(s) 492, 534
TaqII GACCGA 1 cut(s) 75
TasI AATT 7 cut(s) 33, 111, 270, 310, 484, 508, 680
TatI WGTACW 2 cut(s) 476, 546
TauI GCSGC 1 cut(s) 719
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 5 cut(s) 225, 297, 483, 678, 780
Tru9I TTAA 5 cut(s) 225, 297, 483, 678, 780
TscAI CASTG 1 cut(s) 797
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 3 cut(s) 329, 419, 669
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 17, 501
TspRI CASTG 1 cut(s) 797
VpaK11BI GGWCC 2 cut(s) 87, 688
VspI ATTAAT 1 cut(s) 780
XagI CCTNNNNNAGG 1 cut(s) 402
XapI RAATTY 4 cut(s) 33, 310, 508, 680
XmiI GTMKAC 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 1 cut(s) 519
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.