RchiOBHm_Chr3g0487411

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002743
Physical Location & Seq
Forward (+)
34614712 .. 34618002
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45184

Sequence Viewer

Length: 810 bp
ATGAAGGCATTGGCTGTTGTTTGCCCCCCAAGAATTTCCTTCTCCTCCTGCTCAAACTCGTCATGGCAACAACGACACCTTCGGCTTCAGTCCAAACCGAAATCCCAGCTCCGAATTTGCTCACGGTTTCGGGTCTCGTCTTGTTTGATAAAAGAGGGAGAAAGGCAGAAACACAAAGGTGGAGTGGTGGATGAGTTGTTGCTGAATGTGTTCAGGAACAAAATGGTTAAACAAGTGGGATGGGATTCCCATAAGCCGGGATACGATGGACTAATTGAAGTTGCCAACCGGATGATGCTCAACAACCCAACAAATTCTGATACTAAAGAAGCTGCCGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTATAAACTGCTCATAGCTCCTATACAAGGCGGAAAAATAGCCGCCTTCATGGTTGCAAGGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCCTGTACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGGGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGCTGCCAGTTTAAATTCGGGGTCCTTCCTCCACTTCCCGAAGACGACGCCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTACCCGAAGAAGAGAAAGAAACATTAATATAGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

269

Amino Acids

30.19

Weight (kDa)

9.06

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 155 - 236 3.4e-35 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 722
AccI GTMKAC 1 cut(s) 495
AciI CCGC 3 cut(s) 411, 423, 720
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 5 cut(s) 33, 114, 313, 511, 683
AcuI CTGAAG 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 717
AfaI GTAC 2 cut(s) 481, 551
AfiI CCNNNNNNNGG 2 cut(s) 256, 407
AgsI TTSAA 3 cut(s) 278, 619, 661
AhlI ACTAGT 1 cut(s) 521
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 5 cut(s) 109, 332, 398, 598, 672
AluI AGCT 5 cut(s) 109, 332, 398, 598, 672
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 584
AoxI GGCC 1 cut(s) 472
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 332, 672
ApoI RAATTY 5 cut(s) 33, 114, 313, 511, 683
AseI ATTAAT 1 cut(s) 783
Asp700I GAANNNNTTC 1 cut(s) 209
AspS9I GGNCC 3 cut(s) 472, 473, 691
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 454
AvaII GGWCC 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 476
BanII GRGCYC 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 717
BbvI GCAGC 2 cut(s) 319, 659
BccI CCATC 4 cut(s) 234, 260, 500, 640
BceAI ACGGC 1 cut(s) 320
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 521
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 667
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 4 cut(s) 333, 423, 673, 720
BlpI GCTNAGC 1 cut(s) 739
BlsI GCNGC 4 cut(s) 334, 424, 674, 721
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 691
BmgT120I GGNCC 3 cut(s) 472, 473, 691
BmiI GGNNCC 5 cut(s) 474, 475, 651, 692, 732
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 739
BpuEI CTTGAG 1 cut(s) 710
BpuMI CCSGG 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 801
BsaWI WCCGGW 1 cut(s) 288
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 407
Bse1I ACTGG 1 cut(s) 676
BseDI CCNNGG 1 cut(s) 801
BseGI GGATG 3 cut(s) 196, 245, 297
BseLI CCNNNNNNNGG 2 cut(s) 256, 407
BseMII CTCAG 1 cut(s) 620
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 2 cut(s) 319, 659
BseYI CCCAGC 1 cut(s) 105
BshFI GGCC 1 cut(s) 474
BsiSI CCGG 2 cut(s) 257, 289
BslFI GGGAC 2 cut(s) 620, 783
BslI CCNNNNNNNGG 2 cut(s) 256, 407
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 620, 783
BsnI GGCC 1 cut(s) 474
Bso31I GGTCTC 1 cut(s) 139
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 1 cut(s) 576
Bsp1720I GCTNAGC 1 cut(s) 739
BspACI CCGC 3 cut(s) 411, 423, 720
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 619
BspLI GGNNCC 5 cut(s) 474, 475, 651, 692, 732
BspPI GGATC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 722
BsrI ACTGG 1 cut(s) 676
BssECI CCNNGG 1 cut(s) 801
BssMI GATC 1 cut(s) 576
BssNI GRCGYC 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 801
Bst4CI ACNGT 3 cut(s) 126, 381, 484
Bst6I CTCTTC 1 cut(s) 763
BstACI GRCGYC 1 cut(s) 717
BstDEI CTNAG 2 cut(s) 606, 739
BstENI CCTNNNNNAGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 196, 245, 297
BstKTI GATC 1 cut(s) 579
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 1 cut(s) 576
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 1 cut(s) 667
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 2 cut(s) 319, 659
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 1 cut(s) 576
BstYI RGATCY 1 cut(s) 576
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 1 cut(s) 474
BtsCI GGATG 3 cut(s) 196, 245, 297
BtsI GCAGTG 1 cut(s) 800
BtsIMutI CAGTG 1 cut(s) 800
Cfr13I GGNCC 3 cut(s) 472, 473, 691
CseI GACGC 1 cut(s) 725
Csp6I GTAC 2 cut(s) 480, 550
CviAII CATG 5 cut(s) 63, 430, 469, 516, 628
CviQI GTAC 2 cut(s) 480, 550
DdeI CTNAG 2 cut(s) 606, 739
DpnI GATC 1 cut(s) 578
DpnII GATC 1 cut(s) 576
DraI TTTAAA 1 cut(s) 682
Eam1104I CTCTTC 1 cut(s) 763
EarI CTCTTC 1 cut(s) 763
EciI GGCGGA 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 801
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 139
Eco32I GATATC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 691
Eco57I CTGAAG 1 cut(s) 71
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoO109I RGGNCCY 2 cut(s) 473, 691
EcoRI GAATTC 1 cut(s) 511
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 801
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 801
FaeI CATG 5 cut(s) 66, 433, 472, 519, 631
FaqI GGGAC 2 cut(s) 620, 783
FatI CATG 5 cut(s) 62, 429, 468, 515, 627
FauNDI CATATG 1 cut(s) 746
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 4 cut(s) 333, 423, 673, 720
FokI GGATG 3 cut(s) 203, 252, 304
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 4 cut(s) 333, 423, 673, 720
FspBI CTAG 1 cut(s) 522
GluI GCNGC 4 cut(s) 333, 423, 673, 720
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 1 cut(s) 474
HapII CCGG 2 cut(s) 257, 289
HgaI GACGC 1 cut(s) 725
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 5 cut(s) 66, 433, 472, 519, 631
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 1 cut(s) 245
HpaII CCGG 2 cut(s) 257, 289
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 4 cut(s) 113, 319, 341, 348
Hpy188III TCNNGA 3 cut(s) 214, 538, 707
Hpy8I GTNNAC 1 cut(s) 496
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 4 cut(s) 49, 89, 436, 704
HpyCH4III ACNGT 3 cut(s) 126, 381, 484
HpyCH4V TGCA 3 cut(s) 437, 449, 546
HpyF3I CTNAG 2 cut(s) 606, 739
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 5 cut(s) 66, 433, 472, 519, 631
Kzo9I GATC 1 cut(s) 576
LmnI GCTCC 3 cut(s) 114, 403, 730
LpnPI CCDG 8 cut(s) 61, 119, 199, 270, 302, 383, 490, 689
Lsp1109I GCAGC 2 cut(s) 319, 659
LweI GCATC 1 cut(s) 285
MaeI CTAG 1 cut(s) 522
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 578
MbiI CCGCTC 1 cut(s) 722
MboI GATC 1 cut(s) 576
MboII GAAGA 3 cut(s) 722, 777, 780
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 7 cut(s) 33, 114, 273, 313, 487, 511, 683
MmeI TCCRAC 1 cut(s) 626
MnlI CCTC 4 cut(s) 55, 148, 550, 708
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 4 cut(s) 228, 486, 681, 783
MslI CAYNNNNRTG 1 cut(s) 177
MspI CCGG 2 cut(s) 257, 289
MspR9I CCNGG 1 cut(s) 258
NciI CCSGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 746
NdeII GATC 1 cut(s) 576
NlaIII CATG 5 cut(s) 66, 433, 472, 519, 631
NlaIV GGNNCC 5 cut(s) 474, 475, 651, 692, 732
NmuCI GTSAC 1 cut(s) 442
OliI CACNNNNGTG 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 4 cut(s) 334, 424, 674, 721
PpuMI RGGWCCY 1 cut(s) 691
PshBI ATTAAT 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 691
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 5 cut(s) 474, 475, 651, 692, 732
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 3 cut(s) 472, 473, 691
PspPPI RGGWCCY 1 cut(s) 691
PsuI RGATCY 1 cut(s) 576
RsaI GTAC 2 cut(s) 481, 551
RsaNI GTAC 2 cut(s) 480, 550
RseI CAYNNNNRTG 1 cut(s) 177
SalI GTCGAC 1 cut(s) 494
SaqAI TTAA 4 cut(s) 228, 486, 681, 783
SatI GCNGC 4 cut(s) 333, 423, 673, 720
Sau3AI GATC 1 cut(s) 576
Sau96I GGNCC 3 cut(s) 472, 473, 691
ScaI AGTACT 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 476
SetI ASST 8 cut(s) 81, 111, 181, 334, 400, 501, 600, 674
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 667
SinI GGWCC 1 cut(s) 691
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SpeI ACTAGT 1 cut(s) 521
Sse9I AATT 7 cut(s) 33, 114, 273, 313, 487, 511, 683
SsiI CCGC 3 cut(s) 411, 423, 720
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 1 cut(s) 256
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 3 cut(s) 126, 381, 484
TaqI TCGA 2 cut(s) 495, 537
TasI AATT 7 cut(s) 33, 114, 273, 313, 487, 511, 683
TatI WGTACW 2 cut(s) 479, 549
TauI GCSGC 2 cut(s) 425, 722
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 4 cut(s) 228, 486, 681, 783
Tru9I TTAA 4 cut(s) 228, 486, 681, 783
TscAI CASTG 1 cut(s) 800
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 2 cut(s) 332, 672
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 3 cut(s) 17, 418, 504
TspRI CASTG 1 cut(s) 800
VpaK11BI GGWCC 1 cut(s) 691
VspI ATTAAT 1 cut(s) 783
XagI CCTNNNNNAGG 1 cut(s) 405
XapI RAATTY 5 cut(s) 33, 114, 313, 511, 683
XmiI GTMKAC 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 1 cut(s) 522
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.