Rh3AG281900

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002743
Physical Location & Seq
Forward (+)
32878593 .. 32881754
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG281900.1

Sequence Viewer

Length: 579 bp
ATGCTTTGTTCTTGGCAGGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTATAAACTGCTCATAGCTCCTATACAAGGCGGAAAAATAGCCGCCTTCATGGTTGCAAGGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCCTGTACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGGGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGCTGCCAGGTTAACCCAATACCTATAGCCGAATTGACCCATTTCAACAATTTATTGTCCTTAAAAATGACTCACTATTTATCTCAAACTCCGCAGTTTAAATTCGGGGTCCTTCCTCCACTTCCCGAAGACGACGCCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTACCCGAAGAAGAGAAAGAAACATTAATATAGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

192

Amino Acids

21.56

Weight (kDa)

7.45

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 49 - 121 9.1e-32 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccBSI CCGCTC 1 cut(s) 491
AccI GTMKAC 1 cut(s) 177
AciI CCGC 4 cut(s) 93, 105, 443, 489
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 2 cut(s) 193, 452
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 2 cut(s) 163, 233
AfiI CCNNNNNNNGG 1 cut(s) 89
AgsI TTSAA 3 cut(s) 301, 343, 397
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 3 cut(s) 80, 280, 354
AluI AGCT 3 cut(s) 80, 280, 354
AlwI GGATC 1 cut(s) 266
AoxI GGCC 1 cut(s) 154
ApaI GGGCCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 2 cut(s) 193, 452
AseI ATTAAT 1 cut(s) 552
AspS9I GGNCC 4 cut(s) 19, 154, 155, 460
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 2 cut(s) 19, 460
BaeGI GKGCMC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 341
BccI CCATC 2 cut(s) 182, 322
BciT130I CCWGG 1 cut(s) 359
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 1 cut(s) 204
BfmI CTRYAG 2 cut(s) 349, 375
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 3 cut(s) 105, 355, 489
BlpI GCTNAGC 1 cut(s) 508
BlsI GCNGC 3 cut(s) 106, 356, 490
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 359
Bme18I GGWCC 2 cut(s) 19, 460
BmgT120I GGNCC 4 cut(s) 19, 154, 155, 460
BmiI GGNNCC 5 cut(s) 156, 157, 333, 461, 501
BmrFI CCNGG 1 cut(s) 359
BpiI GAAGAC 1 cut(s) 486
Bpu1102I GCTNAGC 1 cut(s) 508
BpuEI CTTGAG 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 89
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 570
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMII CTCAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 1 cut(s) 341
BshFI GGCC 1 cut(s) 156
BslFI GGGAC 2 cut(s) 302, 552
BslI CCNNNNNNNGG 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 302, 552
BsnI GGCC 1 cut(s) 156
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 1 cut(s) 258
Bsp1720I GCTNAGC 1 cut(s) 508
BspACI CCGC 4 cut(s) 93, 105, 443, 489
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 5 cut(s) 156, 157, 333, 461, 501
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 1 cut(s) 258
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 2 cut(s) 63, 166
Bst6I CTCTTC 1 cut(s) 532
BstACI GRCGYC 1 cut(s) 486
BstDEI CTNAG 2 cut(s) 288, 508
BstENI CCTNNNNNAGG 1 cut(s) 87
BstKTI GATC 1 cut(s) 261
BstMBI GATC 1 cut(s) 258
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 357
BstSFI CTRYAG 2 cut(s) 349, 375
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BsuRI GGCC 1 cut(s) 156
BtsI GCAGTG 1 cut(s) 569
BtsIMutI CAGTG 1 cut(s) 569
BveI ACCTGC 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 19, 154, 155, 460
CseI GACGC 1 cut(s) 494
Csp6I GTAC 2 cut(s) 162, 232
CviAII CATG 4 cut(s) 112, 151, 198, 310
CviJI RGCY 9 cut(s) 80, 104, 148, 156, 280, 354, 380, 502, 512
CviKI_1 RGCY 9 cut(s) 80, 104, 148, 156, 280, 354, 380, 502, 512
CviQI GTAC 2 cut(s) 162, 232
DdeI CTNAG 2 cut(s) 288, 508
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
DraI TTTAAA 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 532
EciI GGCGGA 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 158
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 2 cut(s) 19, 460
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 155, 460
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 357
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 4 cut(s) 115, 154, 201, 313
FaqI GGGAC 2 cut(s) 302, 552
FatI CATG 4 cut(s) 111, 150, 197, 309
FauNDI CATATG 1 cut(s) 515
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 105, 355, 489
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 3 cut(s) 105, 355, 489
FspBI CTAG 1 cut(s) 204
GluI GCNGC 3 cut(s) 105, 355, 489
HaeIII GGCC 1 cut(s) 156
HgaI GACGC 1 cut(s) 494
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 4 cut(s) 115, 154, 201, 313
HincII GTYRAC 2 cut(s) 178, 364
HindII GTYRAC 2 cut(s) 178, 364
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 1 cut(s) 421
HpaI GTTAAC 1 cut(s) 364
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 2 cut(s) 178, 364
Hpy188I TCNGA 2 cut(s) 23, 30
Hpy188III TCNNGA 2 cut(s) 220, 476
Hpy8I GTNNAC 2 cut(s) 178, 364
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 2 cut(s) 118, 473
HpyCH4III ACNGT 2 cut(s) 63, 166
HpyCH4V TGCA 3 cut(s) 119, 131, 228
HpyF3I CTNAG 2 cut(s) 288, 508
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 4 cut(s) 115, 154, 201, 313
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 258
LmnI GCTCC 2 cut(s) 85, 499
LpnPI CCDG 5 cut(s) 2, 65, 172, 344, 371
Lsp1109I GCAGC 1 cut(s) 341
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 260
MbiI CCGCTC 1 cut(s) 491
MboI GATC 1 cut(s) 258
MboII GAAGA 3 cut(s) 491, 546, 549
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 5 cut(s) 169, 193, 383, 400, 452
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 2 cut(s) 232, 477
MseI TTAA 5 cut(s) 168, 363, 413, 450, 552
MspR9I CCNGG 1 cut(s) 359
MvaI CCWGG 1 cut(s) 359
NdeI CATATG 1 cut(s) 515
NdeII GATC 1 cut(s) 258
NlaIII CATG 4 cut(s) 115, 154, 201, 313
NlaIV GGNNCC 5 cut(s) 156, 157, 333, 461, 501
NmuCI GTSAC 1 cut(s) 124
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PkrI GCNGC 3 cut(s) 106, 356, 490
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PpuMI RGGWCCY 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 5 cut(s) 156, 157, 333, 461, 501
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 19, 154, 155, 460
PspPPI RGGWCCY 1 cut(s) 460
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 2 cut(s) 163, 233
RsaNI GTAC 2 cut(s) 162, 232
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 5 cut(s) 168, 363, 413, 450, 552
SatI GCNGC 3 cut(s) 105, 355, 489
Sau3AI GATC 1 cut(s) 258
Sau96I GGNCC 4 cut(s) 19, 154, 155, 460
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 1 cut(s) 158
SetI ASST 7 cut(s) 21, 82, 183, 282, 356, 363, 376
SfcI CTRYAG 2 cut(s) 349, 375
SinI GGWCC 2 cut(s) 19, 460
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SpeI ACTAGT 1 cut(s) 203
Sse9I AATT 5 cut(s) 169, 193, 383, 400, 452
SsiI CCGC 4 cut(s) 93, 105, 443, 489
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 357
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 63, 166
TaqI TCGA 2 cut(s) 177, 219
TasI AATT 5 cut(s) 169, 193, 383, 400, 452
TatI WGTACW 2 cut(s) 161, 231
TauI GCSGC 2 cut(s) 107, 491
Tru1I TTAA 5 cut(s) 168, 363, 413, 450, 552
Tru9I TTAA 5 cut(s) 168, 363, 413, 450, 552
TscAI CASTG 1 cut(s) 569
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 354
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 2 cut(s) 100, 186
TspRI CASTG 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 19, 460
VspI ATTAAT 1 cut(s) 552
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 2 cut(s) 193, 452
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 1 cut(s) 204
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.