RLG00000023005

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002743
Physical Location & Seq
Reverse (-)
16592969 .. 16595286
2318 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023005

Sequence Viewer

Length: 513 bp
ATGACTGAGAAGTACTTGGCTACGATAGAGATCCACAAGGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTATAAACTGCTTATAGCTCCTATACAAGGCGGAAAAATAGCCGCCCTCATGGTTGCAAGGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCTTGCACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGTGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCAAACTTCAACTACTATAGCTGCCAGTTTAAATTCGGGGTCCTTCCTCCACTTCCCGAAGATGACACCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTGCCCGAAGAAGAGAAAGAAACATTAATATAGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

171

Amino Acids

19.08

Weight (kDa)

8.01

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 56 - 137 2e-34 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 198
AciI CCGC 3 cut(s) 114, 126, 423
AclWI GGATC 2 cut(s) 25, 287
AcsI RAATTY 2 cut(s) 214, 386
AfaI GTAC 2 cut(s) 14, 254
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 2 cut(s) 322, 364
AhlI ACTAGT 1 cut(s) 224
AluBI AGCT 3 cut(s) 101, 301, 375
AluI AGCT 3 cut(s) 101, 301, 375
AlwI GGATC 2 cut(s) 25, 287
AoxI GGCC 1 cut(s) 175
ApaI GGGCCC 1 cut(s) 179
ApeKI GCWGC 2 cut(s) 375, 461
ApoI RAATTY 2 cut(s) 214, 386
AseI ATTAAT 1 cut(s) 486
AspS9I GGNCC 4 cut(s) 40, 175, 176, 394
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 2 cut(s) 40, 394
BaeGI GKGCMC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 179
BbvI GCAGC 2 cut(s) 362, 448
BccI CCATC 2 cut(s) 203, 343
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 370
BisI GCNGC 3 cut(s) 126, 376, 462
BlpI GCTNAGC 1 cut(s) 442
BlsI GCNGC 3 cut(s) 127, 377, 463
BmcAI AGTACT 2 cut(s) 14, 254
Bme18I GGWCC 2 cut(s) 40, 394
BmgT120I GGNCC 4 cut(s) 40, 175, 176, 394
BmiI GGNNCC 4 cut(s) 177, 354, 395, 435
Bpu1102I GCTNAGC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 504
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 379
Bse8I GATNNNNATC 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 504
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 379
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 2 cut(s) 362, 448
BshFI GGCC 1 cut(s) 177
BslFI GGGAC 2 cut(s) 323, 486
BslI CCNNNNNNNGG 1 cut(s) 110
BsmFI GGGAC 2 cut(s) 323, 486
BsnI GGCC 1 cut(s) 177
Bsp120I GGGCCC 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 2 cut(s) 30, 279
Bsp1720I GCTNAGC 1 cut(s) 442
BspACI CCGC 3 cut(s) 114, 126, 423
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 1 cut(s) 322
BspLI GGNNCC 4 cut(s) 177, 354, 395, 435
BspPI GGATC 2 cut(s) 25, 287
BsrBI CCGCTC 1 cut(s) 425
BsrI ACTGG 1 cut(s) 379
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 2 cut(s) 30, 279
BssT1I CCWWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 84, 187
Bst6I CTCTTC 1 cut(s) 466
BstDEI CTNAG 3 cut(s) 6, 309, 442
BstENI CCTNNNNNAGG 1 cut(s) 108
BstKTI GATC 2 cut(s) 33, 282
BstMBI GATC 2 cut(s) 30, 279
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 2 cut(s) 362, 448
BstX2I RGATCY 2 cut(s) 30, 279
BstYI RGATCY 2 cut(s) 30, 279
BsuRI GGCC 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 2 cut(s) 183, 503
Cfr13I GGNCC 4 cut(s) 40, 175, 176, 394
Csp6I GTAC 2 cut(s) 13, 253
CviAII CATG 4 cut(s) 133, 172, 219, 331
CviJI RGCY 9 cut(s) 20, 101, 125, 169, 177, 301, 375, 436, 446
CviKI_1 RGCY 9 cut(s) 20, 101, 125, 169, 177, 301, 375, 436, 446
CviQI GTAC 2 cut(s) 13, 253
DdeI CTNAG 3 cut(s) 6, 309, 442
DpnI GATC 2 cut(s) 32, 281
DpnII GATC 2 cut(s) 30, 279
DraI TTTAAA 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 179
Eco32I GATATC 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 40, 394
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 2 cut(s) 176, 394
EcoRI GAATTC 1 cut(s) 214
EcoRV GATATC 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 504
FaeI CATG 4 cut(s) 136, 175, 222, 334
FaqI GGGAC 2 cut(s) 323, 486
FatI CATG 4 cut(s) 132, 171, 218, 330
FauNDI CATATG 1 cut(s) 449
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 126, 376, 462
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 3 cut(s) 126, 376, 462
FspBI CTAG 1 cut(s) 225
GluI GCNGC 3 cut(s) 126, 376, 462
HaeIII GGCC 1 cut(s) 177
Hin1II CATG 4 cut(s) 136, 175, 222, 334
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 299
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 2 cut(s) 44, 51
Hpy188III TCNNGA 2 cut(s) 241, 410
Hpy8I GTNNAC 1 cut(s) 199
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 2 cut(s) 84, 187
HpyCH4V TGCA 4 cut(s) 140, 152, 183, 249
HpyF3I CTNAG 3 cut(s) 6, 309, 442
Hsp92II CATG 4 cut(s) 136, 175, 222, 334
Kzo9I GATC 2 cut(s) 30, 279
LmnI GCTCC 2 cut(s) 106, 433
LpnPI CCDG 2 cut(s) 86, 392
Lsp1109I GCAGC 2 cut(s) 362, 448
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 145
MalI GATC 2 cut(s) 32, 281
MbiI CCGCTC 1 cut(s) 425
MboI GATC 2 cut(s) 30, 279
MboII GAAGA 3 cut(s) 425, 480, 483
MflI RGATCY 2 cut(s) 30, 279
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 3 cut(s) 190, 214, 386
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 3 cut(s) 140, 253, 411
MseI TTAA 3 cut(s) 189, 384, 486
NdeI CATATG 1 cut(s) 449
NdeII GATC 2 cut(s) 30, 279
NlaIII CATG 4 cut(s) 136, 175, 222, 334
NlaIV GGNNCC 4 cut(s) 177, 354, 395, 435
NmuCI GTSAC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PkrI GCNGC 3 cut(s) 127, 377, 463
PpuMI RGGWCCY 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 394
PspN4I GGNNCC 4 cut(s) 177, 354, 395, 435
PspOMI GGGCCC 1 cut(s) 175
PspPI GGNCC 4 cut(s) 40, 175, 176, 394
PspPPI RGGWCCY 1 cut(s) 394
PsuI RGATCY 2 cut(s) 30, 279
RsaI GTAC 2 cut(s) 14, 254
RsaNI GTAC 2 cut(s) 13, 253
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 3 cut(s) 189, 384, 486
SatI GCNGC 3 cut(s) 126, 376, 462
Sau3AI GATC 2 cut(s) 30, 279
Sau96I GGNCC 4 cut(s) 40, 175, 176, 394
ScaI AGTACT 2 cut(s) 14, 254
SduI GDGCHC 1 cut(s) 179
SetI ASST 5 cut(s) 42, 103, 204, 303, 377
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 2 cut(s) 40, 394
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 3 cut(s) 190, 214, 386
SsiI CCGC 3 cut(s) 114, 126, 423
SspMI CTAG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 84, 187
TaqI TCGA 2 cut(s) 198, 240
TasI AATT 3 cut(s) 190, 214, 386
TatI WGTACW 2 cut(s) 12, 252
TauI GCSGC 1 cut(s) 128
Tru1I TTAA 3 cut(s) 189, 384, 486
Tru9I TTAA 3 cut(s) 189, 384, 486
TscAI CASTG 2 cut(s) 190, 503
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 375, 461
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 207
TspRI CASTG 2 cut(s) 190, 503
VpaK11BI GGWCC 2 cut(s) 40, 394
VspI ATTAAT 1 cut(s) 486
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 2 cut(s) 214, 386
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 225
ZrmI AGTACT 2 cut(s) 14, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.