Rh3BG317500

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002743
Physical Location & Seq
Forward (+)
32517032 .. 32519995
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG317500.1

Sequence Viewer

Length: 819 bp
ATGAAGGCTTTGGCTGTTGTTTGCCCCCCAAGAATTTCCTTCTCCTGCTCCTCCTCATGGCAACAACAACACCTTCGGTTTCGGTCCAAACCGAAATCCCAGCTCCGAATTAGCTCACTGTTTCGGGTCTCGTCGTGTTTGATAAAAGAGGGAGAAAGCGAATCAGAAAGGCAGAAATACAAAGGTGGTGGAGTGGTGGATGAGTTGTTGCTGAATGTGTTCAGGAACAAAATGGTTAAACAAGTGGGATGGGATTCCGATAAGCCGGGATACGATGGACTAATTGAAGTTGCCAACCGGATGATGCTCAACAACCCAACAAATTCTGATACTAAACAAGCTGCGGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTACAAACTGCTTATAGCTCCTATACAAGGCGGAAAAATAGCCGCCTTCATGGTTGCAAGGGTGACTGCAATCAGCTGTCAATGGCTCATGGGCCCCTGCACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGGGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGCTGCCAGTTTAAATTCGGGGTCCTTCCTCCACTTTCCGAAGACGACGCCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTACCCGAAGAAGAGAAAGAAACATTAATATGGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

272

Amino Acids

30.47

Weight (kDa)

8.84

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 158 - 239 3.3e-35 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 731
AccI GTMKAC 1 cut(s) 504
AciI CCGC 4 cut(s) 344, 420, 432, 729
AclWI GGATC 1 cut(s) 593
AcsI RAATTY 4 cut(s) 33, 322, 520, 692
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 2 cut(s) 392, 560
AfiI CCNNNNNNNGG 2 cut(s) 57, 416
AgsI TTSAA 3 cut(s) 287, 628, 670
AhlI ACTAGT 1 cut(s) 530
AluBI AGCT 7 cut(s) 103, 114, 341, 407, 465, 607, 681
AluI AGCT 7 cut(s) 103, 114, 341, 407, 465, 607, 681
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 1 cut(s) 593
AoxI GGCC 1 cut(s) 481
ApaI GGGCCC 1 cut(s) 485
ApeKI GCWGC 2 cut(s) 341, 681
ApoI RAATTY 4 cut(s) 33, 322, 520, 692
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 218
AspS9I GGNCC 5 cut(s) 84, 346, 481, 482, 700
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 3 cut(s) 84, 346, 700
BaeGI GKGCMC 1 cut(s) 485
BanII GRGCYC 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 726
BbvI GCAGC 2 cut(s) 328, 668
BccI CCATC 4 cut(s) 243, 269, 509, 649
BciVI GTATCC 1 cut(s) 263
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 1 cut(s) 531
BfmI CTRYAG 1 cut(s) 676
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 4 cut(s) 342, 432, 682, 729
BlpI GCTNAGC 1 cut(s) 748
BlsI GCNGC 4 cut(s) 343, 433, 683, 730
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 3 cut(s) 84, 346, 700
BmgT120I GGNCC 5 cut(s) 84, 346, 481, 482, 700
BmiI GGNNCC 5 cut(s) 483, 484, 660, 701, 741
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 726
Bpu1102I GCTNAGC 1 cut(s) 748
BpuEI CTTGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 726
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 810
BsaWI WCCGGW 1 cut(s) 297
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 416
Bse1I ACTGG 1 cut(s) 685
BseDI CCNNGG 1 cut(s) 810
BseGI GGATG 3 cut(s) 205, 254, 306
BseLI CCNNNNNNNGG 2 cut(s) 57, 416
BseMII CTCAG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 2 cut(s) 40, 43
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 2 cut(s) 328, 668
BseYI CCCAGC 1 cut(s) 99
BsgI GTGCAG 1 cut(s) 472
BshFI GGCC 1 cut(s) 483
BsiSI CCGG 2 cut(s) 266, 298
BslFI GGGAC 2 cut(s) 629, 792
BslI CCNNNNNNNGG 2 cut(s) 57, 416
BsmAI GTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 629, 792
BsnI GGCC 1 cut(s) 483
Bso31I GGTCTC 1 cut(s) 133
Bsp120I GGGCCC 1 cut(s) 481
Bsp1286I GDGCHC 1 cut(s) 485
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 1 cut(s) 585
Bsp1720I GCTNAGC 1 cut(s) 748
BspACI CCGC 4 cut(s) 344, 420, 432, 729
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 628
BspLI GGNNCC 5 cut(s) 483, 484, 660, 701, 741
BspPI GGATC 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 133
BsrBI CCGCTC 1 cut(s) 731
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 1 cut(s) 685
BssECI CCNNGG 1 cut(s) 810
BssMI GATC 1 cut(s) 585
BssNI GRCGYC 1 cut(s) 726
BssT1I CCWWGG 1 cut(s) 810
Bst4CI ACNGT 3 cut(s) 120, 390, 493
Bst6I CTCTTC 1 cut(s) 772
BstACI GRCGYC 1 cut(s) 726
BstAUI TGTACA 1 cut(s) 390
BstDEI CTNAG 2 cut(s) 615, 748
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 3 cut(s) 205, 254, 306
BstKTI GATC 1 cut(s) 588
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 585
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 676
BstSLI GKGCMC 1 cut(s) 485
BstV1I GCAGC 2 cut(s) 328, 668
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 1 cut(s) 585
BstYI RGATCY 1 cut(s) 585
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 1 cut(s) 483
BtsCI GGATG 3 cut(s) 205, 254, 306
BtsI GCAGTG 1 cut(s) 809
BtsIMutI CAGTG 3 cut(s) 116, 489, 809
Cfr13I GGNCC 5 cut(s) 84, 346, 481, 482, 700
CpoI CGGWCCG 1 cut(s) 346
CseI GACGC 1 cut(s) 734
Csp6I GTAC 2 cut(s) 391, 559
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CspI CGGWCCG 1 cut(s) 346
CviAII CATG 5 cut(s) 57, 439, 478, 525, 637
CviQI GTAC 2 cut(s) 391, 559
DdeI CTNAG 2 cut(s) 615, 748
DpnI GATC 1 cut(s) 587
DpnII GATC 1 cut(s) 585
DraI TTTAAA 1 cut(s) 691
Eam1104I CTCTTC 1 cut(s) 772
EarI CTCTTC 1 cut(s) 772
EciI GGCGGA 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 810
Eco24I GRGCYC 1 cut(s) 485
Eco31I GGTCTC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 3 cut(s) 84, 346, 700
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoO109I RGGNCCY 2 cut(s) 482, 700
EcoRI GAATTC 1 cut(s) 520
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 810
EcoT38I GRGCYC 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 810
FaeI CATG 5 cut(s) 60, 442, 481, 528, 640
FaqI GGGAC 2 cut(s) 629, 792
FatI CATG 5 cut(s) 56, 438, 477, 524, 636
FauNDI CATATG 1 cut(s) 755
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 4 cut(s) 342, 432, 682, 729
FokI GGATG 3 cut(s) 212, 261, 313
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 4 cut(s) 342, 432, 682, 729
FspBI CTAG 1 cut(s) 531
GluI GCNGC 4 cut(s) 342, 432, 682, 729
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 483
HapII CCGG 2 cut(s) 266, 298
HgaI GACGC 1 cut(s) 734
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 5 cut(s) 60, 442, 481, 528, 640
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 2 cut(s) 161, 254
HpaII CCGG 2 cut(s) 266, 298
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 7 cut(s) 107, 166, 259, 328, 350, 357, 718
Hpy188III TCNNGA 2 cut(s) 223, 547
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 2 cut(s) 136, 728
HpyAV CCTTC 4 cut(s) 49, 83, 445, 713
HpyCH4III ACNGT 3 cut(s) 120, 390, 493
HpyCH4V TGCA 4 cut(s) 446, 458, 489, 555
HpyF3I CTNAG 2 cut(s) 615, 748
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 5 cut(s) 60, 442, 481, 528, 640
Kzo9I GATC 1 cut(s) 585
LmnI GCTCC 5 cut(s) 53, 108, 412, 739, 799
LpnPI CCDG 8 cut(s) 58, 113, 208, 279, 311, 392, 499, 698
Lsp1109I GCAGC 2 cut(s) 328, 668
LweI GCATC 1 cut(s) 294
MaeI CTAG 1 cut(s) 531
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 587
MbiI CCGCTC 1 cut(s) 731
MboI GATC 1 cut(s) 585
MboII GAAGA 3 cut(s) 731, 786, 789
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 7 cut(s) 33, 108, 282, 322, 496, 520, 692
MmeI TCCRAC 1 cut(s) 635
MnlI CCTC 5 cut(s) 61, 64, 142, 559, 717
MroXI GAANNNNTTC 1 cut(s) 218
MseI TTAA 4 cut(s) 237, 495, 690, 792
MslI CAYNNNNRTG 1 cut(s) 794
MspA1I CMGCKG 1 cut(s) 465
MspI CCGG 2 cut(s) 266, 298
MspR9I CCNGG 1 cut(s) 267
NciI CCSGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 755
NdeII GATC 1 cut(s) 585
NlaIII CATG 5 cut(s) 60, 442, 481, 528, 640
NlaIV GGNNCC 5 cut(s) 483, 484, 660, 701, 741
NmuCI GTSAC 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 161, 254
PkrI GCNGC 4 cut(s) 343, 433, 683, 730
PpuMI RGGWCCY 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 792
Psp5II RGGWCCY 1 cut(s) 700
PspFI CCCAGC 1 cut(s) 99
PspN4I GGNNCC 5 cut(s) 483, 484, 660, 701, 741
PspOMI GGGCCC 1 cut(s) 481
PspPI GGNCC 5 cut(s) 84, 346, 481, 482, 700
PspPPI RGGWCCY 1 cut(s) 700
PsuI RGATCY 1 cut(s) 585
PvuII CAGCTG 1 cut(s) 465
RsaI GTAC 2 cut(s) 392, 560
RsaNI GTAC 2 cut(s) 391, 559
RseI CAYNNNNRTG 1 cut(s) 794
Rsr2I CGGWCCG 1 cut(s) 346
RsrII CGGWCCG 1 cut(s) 346
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 4 cut(s) 237, 495, 690, 792
SatI GCNGC 4 cut(s) 342, 432, 682, 729
Sau3AI GATC 1 cut(s) 585
Sau96I GGNCC 5 cut(s) 84, 346, 481, 482, 700
ScaI AGTACT 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 1 cut(s) 294
SfcI CTRYAG 1 cut(s) 676
SinI GGWCC 3 cut(s) 84, 346, 700
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
SpeI ACTAGT 1 cut(s) 530
Sse9I AATT 7 cut(s) 33, 108, 282, 322, 496, 520, 692
SsiI CCGC 4 cut(s) 344, 420, 432, 729
SspMI CTAG 1 cut(s) 531
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 3 cut(s) 120, 390, 493
TaqI TCGA 2 cut(s) 504, 546
TaqII GACCGA 1 cut(s) 72
TasI AATT 7 cut(s) 33, 108, 282, 322, 496, 520, 692
TatI WGTACW 2 cut(s) 390, 558
TauI GCSGC 2 cut(s) 434, 731
TfiI GAWTC 2 cut(s) 161, 254
Tru1I TTAA 4 cut(s) 237, 495, 690, 792
Tru9I TTAA 4 cut(s) 237, 495, 690, 792
TscAI CASTG 3 cut(s) 123, 496, 809
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 341, 681
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 3 cut(s) 17, 427, 513
TspRI CASTG 3 cut(s) 123, 496, 809
VpaK11BI GGWCC 3 cut(s) 84, 346, 700
VspI ATTAAT 1 cut(s) 792
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 4 cut(s) 33, 322, 520, 692
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 1 cut(s) 531
ZrmI AGTACT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.