FvH4_1g27090

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002743
Physical Location & Seq
Forward (+)
18855969 .. 18859062
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27090.t14

Sequence Viewer

Length: 801 bp
ATGAAGGCTTTGGCGCTTGTTTGCCCCGCAAGCATTTCCTCCTCGTGGCTTCCGTTCCGGTTTGGGCCCCAATCGAAATCTCAGCTCCGGAGCTCACGGATTCGGATCTTCTCTTGTTTGACGAAAGAGGCAGAAAGCGAATCGCAATCGGGGAGTTACAAGGGAGGAGTGGTGGATAAGTTGTTGCTGAATGTGTTCAGGAACAAAATGGTTCAACAAGTTGGGTGGGATTCGGATAAGCCGGGATACGATGGACTGATTGAAGTGGCCAACCGGATGATGCTTCAGAACCCAACAAATTCTCATACTAAAGAAGCTGCGGTCCGAGTTCTGATATCTCTGTTTCCACCATTCCTGTTAGAACTTTATAAATTGCTAATAGCTCCTATACAAGGCGGGAAAATAGCCGCGCTCATGGTTGCAAGGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCCTGCACTGTTAATTCTGTCGACCTACCTGATGGGAACTCATGGGCTAGTGGGGTGTTTGTTGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTGGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGTTGTCAGTTTAAATTCGGGGTCCTTCCTCCACTTCCCGAAGACGATGCCACTCTCAAGGAGCCTTGCTTAGCAATATGCCCTAATGCTACCCGAAGAAGAGAAATTGCAAAAAACTTTAATGTAGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

267

Amino Acids

29.49

Weight (kDa)

8.68

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 150 - 198 1.8e-17 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
AccI GTMKAC 1 cut(s) 480
AccII CGCG 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 87
AciI CCGC 4 cut(s) 27, 320, 396, 408
AclWI GGATC 2 cut(s) 113, 569
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 2 cut(s) 298, 668
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 2 cut(s) 45, 392
AgsI TTSAA 4 cut(s) 215, 263, 604, 646
AluBI AGCT 5 cut(s) 85, 93, 317, 383, 583
AluI AGCT 5 cut(s) 85, 93, 317, 383, 583
Alw21I GWGCWC 1 cut(s) 95
AlwI GGATC 2 cut(s) 113, 569
Aor13HI TCCGGA 1 cut(s) 87
AoxI GGCC 3 cut(s) 65, 267, 457
ApaI GGGCCC 2 cut(s) 69, 461
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 2 cut(s) 298, 668
Asp700I GAANNNNTTC 1 cut(s) 194
AspLEI GCGC 2 cut(s) 16, 412
AspS9I GGNCC 6 cut(s) 65, 66, 322, 457, 458, 676
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 439
AvaII GGWCC 2 cut(s) 322, 676
BaeGI GKGCMC 2 cut(s) 69, 461
BalI TGGCCA 1 cut(s) 269
BanII GRGCYC 3 cut(s) 69, 95, 461
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 304
BccI CCATC 3 cut(s) 245, 485, 625
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciVI GTATCC 1 cut(s) 239
BcnI CCSGG 1 cut(s) 243
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 652
BfoI RGCGCY 1 cut(s) 17
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 2 cut(s) 318, 408
BlpI GCTNAGC 1 cut(s) 724
BlsI GCNGC 2 cut(s) 319, 409
BmcAI AGTACT 1 cut(s) 536
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 2 cut(s) 322, 676
BmgT120I GGNCC 6 cut(s) 65, 66, 322, 457, 458, 676
BmiI GGNNCC 7 cut(s) 67, 68, 459, 460, 636, 677, 717
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 2 cut(s) 270, 691
BpiI GAAGAC 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 724
BpuEI CTTGAG 1 cut(s) 695
BpuMI CCSGG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 792
BsaWI WCCGGW 3 cut(s) 57, 87, 273
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 392
Bse8I GATNNNNATC 1 cut(s) 104
BseAI TCCGGA 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 792
BseGI GGATG 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 104
BseLI CCNNNNNNNGG 2 cut(s) 45, 392
BseMII CTCAG 2 cut(s) 95, 605
BseRI GAGGAG 2 cut(s) 31, 180
BseSI GKGCMC 2 cut(s) 69, 461
BseXI GCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 410
BshFI GGCC 3 cut(s) 67, 269, 459
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 4 cut(s) 58, 88, 242, 274
BslFI GGGAC 2 cut(s) 605, 774
BslI CCNNNNNNNGG 2 cut(s) 45, 392
BsmFI GGGAC 2 cut(s) 605, 774
BsnI GGCC 3 cut(s) 67, 269, 459
Bsp120I GGGCCC 2 cut(s) 65, 457
Bsp1286I GDGCHC 3 cut(s) 69, 95, 461
Bsp13I TCCGGA 1 cut(s) 87
Bsp143I GATC 2 cut(s) 105, 561
Bsp1720I GCTNAGC 1 cut(s) 724
BspACI CCGC 4 cut(s) 27, 320, 396, 408
BspANI GGCC 3 cut(s) 67, 269, 459
BspCNI CTCAG 2 cut(s) 94, 604
BspEI TCCGGA 1 cut(s) 87
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 7 cut(s) 67, 68, 459, 460, 636, 677, 717
BspPI GGATC 2 cut(s) 113, 569
BssECI CCNNGG 1 cut(s) 792
BssMI GATC 2 cut(s) 105, 561
BssSI CACGAG 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 792
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 1 cut(s) 748
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 3 cut(s) 81, 591, 724
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 1 cut(s) 282
BstFNI CGCG 1 cut(s) 410
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 2 cut(s) 16, 412
BstKTI GATC 2 cut(s) 108, 564
BstMBI GATC 2 cut(s) 105, 561
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 241
BstSFI CTRYAG 1 cut(s) 652
BstSLI GKGCMC 2 cut(s) 69, 461
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 2 cut(s) 105, 561
BstYI RGATCY 2 cut(s) 105, 561
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 3 cut(s) 67, 269, 459
BtsCI GGATG 1 cut(s) 282
BtsI GCAGTG 1 cut(s) 791
BtsIMutI CAGTG 2 cut(s) 465, 791
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 2 cut(s) 16, 412
Cfr13I GGNCC 6 cut(s) 65, 66, 322, 457, 458, 676
CpoI CGGWCCG 1 cut(s) 322
Csp6I GTAC 1 cut(s) 535
CspCI CAANNNNNGTGG 2 cut(s) 206, 241
CspI CGGWCCG 1 cut(s) 322
CviAII CATG 4 cut(s) 415, 454, 501, 613
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 3 cut(s) 81, 591, 724
DpnI GATC 2 cut(s) 107, 563
DpnII GATC 2 cut(s) 105, 561
DraI TTTAAA 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 748
EarI CTCTTC 1 cut(s) 748
Ecl136II GAGCTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 792
Eco24I GRGCYC 3 cut(s) 69, 95, 461
Eco32I GATATC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 322, 676
Eco53kI GAGCTC 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 93
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoO109I RGGNCCY 3 cut(s) 66, 458, 676
EcoRV GATATC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 792
EcoT38I GRGCYC 3 cut(s) 69, 95, 461
ErhI CCWWGG 1 cut(s) 792
FaeI CATG 4 cut(s) 418, 457, 504, 616
FaqI GGGAC 2 cut(s) 605, 774
FatI CATG 4 cut(s) 414, 453, 500, 612
FauI CCCGC 2 cut(s) 34, 389
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 2 cut(s) 318, 408
FokI GGATG 1 cut(s) 289
FriOI GRGCYC 3 cut(s) 69, 95, 461
Fsp4HI GCNGC 2 cut(s) 318, 408
FspBI CTAG 1 cut(s) 507
GlaI GCGC 2 cut(s) 15, 411
GluI GCNGC 2 cut(s) 318, 408
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 3 cut(s) 67, 269, 459
HapII CCGG 4 cut(s) 58, 88, 242, 274
HhaI GCGC 2 cut(s) 16, 412
Hin1II CATG 4 cut(s) 418, 457, 504, 616
Hin6I GCGC 2 cut(s) 14, 410
HinP1I GCGC 2 cut(s) 14, 410
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 3 cut(s) 100, 140, 230
HpaII CCGG 4 cut(s) 58, 88, 242, 274
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 5 cut(s) 105, 235, 288, 326, 333
Hpy188III TCNNGA 3 cut(s) 88, 199, 692
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 1 cut(s) 689
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 5 cut(s) 422, 434, 465, 531, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 3 cut(s) 81, 591, 724
Hsp92II CATG 4 cut(s) 418, 457, 504, 616
HspAI GCGC 2 cut(s) 14, 410
Kpn2I TCCGGA 1 cut(s) 87
Kzo9I GATC 2 cut(s) 105, 561
LmnI GCTCC 4 cut(s) 90, 90, 388, 715
LpnPI CCDG 8 cut(s) 71, 101, 184, 255, 287, 368, 475, 501
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 2 cut(s) 270, 691
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 2 cut(s) 155, 427
MalI GATC 2 cut(s) 107, 563
MboI GATC 2 cut(s) 105, 561
MboII GAAGA 4 cut(s) 100, 707, 762, 765
MflI RGATCY 2 cut(s) 105, 561
MhlI GDGCHC 3 cut(s) 69, 95, 461
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 5 cut(s) 298, 371, 472, 668, 759
MluNI TGGCCA 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 611
MnlI CCTC 6 cut(s) 49, 52, 121, 158, 535, 693
Mox20I TGGCCA 1 cut(s) 269
MroI TCCGGA 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 194
MscI TGGCCA 1 cut(s) 269
MseI TTAA 3 cut(s) 471, 666, 774
Msp20I TGGCCA 1 cut(s) 269
MspI CCGG 4 cut(s) 58, 88, 242, 274
MspR9I CCNGG 1 cut(s) 243
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 30
NciI CCSGG 1 cut(s) 243
NdeII GATC 2 cut(s) 105, 561
NlaIII CATG 4 cut(s) 418, 457, 504, 616
NlaIV GGNNCC 7 cut(s) 67, 68, 459, 460, 636, 677, 717
NmuCI GTSAC 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 3 cut(s) 100, 140, 230
PkrI GCNGC 2 cut(s) 319, 409
PpuMI RGGWCCY 1 cut(s) 676
PsiI TTATAA 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 676
PspN4I GGNNCC 7 cut(s) 67, 68, 459, 460, 636, 677, 717
PspOMI GGGCCC 2 cut(s) 65, 457
PspPI GGNCC 6 cut(s) 65, 66, 322, 457, 458, 676
PspPPI RGGWCCY 1 cut(s) 676
PsuI RGATCY 2 cut(s) 105, 561
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
Rsr2I CGGWCCG 1 cut(s) 322
RsrII CGGWCCG 1 cut(s) 322
SacI GAGCTC 1 cut(s) 95
SalI GTCGAC 1 cut(s) 479
SaqAI TTAA 3 cut(s) 471, 666, 774
SatI GCNGC 2 cut(s) 318, 408
Sau3AI GATC 2 cut(s) 105, 561
Sau96I GGNCC 6 cut(s) 65, 66, 322, 457, 458, 676
ScaI AGTACT 1 cut(s) 536
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 3 cut(s) 69, 95, 461
SetI ASST 7 cut(s) 87, 95, 319, 385, 486, 490, 585
SfaNI GCATC 2 cut(s) 270, 691
SfcI CTRYAG 1 cut(s) 652
SinI GGWCC 2 cut(s) 322, 676
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
Sse9I AATT 5 cut(s) 298, 371, 472, 668, 759
SsiI CCGC 4 cut(s) 27, 320, 396, 408
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 2 cut(s) 74, 480
TasI AATT 5 cut(s) 298, 371, 472, 668, 759
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 3 cut(s) 100, 140, 230
Tru1I TTAA 3 cut(s) 471, 666, 774
Tru9I TTAA 3 cut(s) 471, 666, 774
TscAI CASTG 2 cut(s) 472, 791
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 42, 112
TspRI CASTG 2 cut(s) 472, 791
VpaK11BI GGWCC 2 cut(s) 322, 676
XagI CCTNNNNNAGG 1 cut(s) 390
XapI RAATTY 2 cut(s) 298, 668
XmiI GTMKAC 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 1 cut(s) 507
ZrmI AGTACT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.