AT4G02405

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011175
Physical Location & Seq
Reverse (-)
1057854 .. 1059671
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02405.2

Sequence Viewer

Length: 1002 bp
ATGATCTACTTGAAACCTCTGGTTCCGTCAGAGGTGTTAGCTTCGTCTTCATTCAAAAAGTCAGCGATATCATTACGGATGCAGAGTCTATGTGCTCCTCCTCCTGTACACTTTCCGGCCACCGCAATCCGTCGCTGTCGAACTCCGACGCTCGCCGTTCGTTCCTTACGAGCAGAGAATGAGCCTTCGATTCAATCAGCTTTAGTTTCAGCGTCTCATCGTCTGCGAGAAACCAATCGAACAGAGCCTCTTTTCATCGACCCGTATGCCGCTTGTTTCTTACCTCCTTACACAAAGAAGGATTTGGACATCCATGAACAACAACAACATTACTGTCTTGCAACAAAGTTCATCGATGATAAGTTGTTAGAAATAGCTAAACGCATTGATGGGCTAAAGCAGGTTGTATTGTTCACAGATGGGATGGATACTCGACCTTATAGGCTTAATTGGCCAACTTCCACTATGATCTTTGATGTATCACCGGAAAAAGTTTTCGAAATAGCATCTGAAAAGCTTCAAGGCGTGGGAGCTAGGATCCCTAAAAGTTGCTTATTCTTTCACATTCCCGTGGAATTCGAGAACATAGAGCAACGTTTACGCTCAAAAGGGTTTAGTGGGAATCGGCCTAGTATATGGGCAATGCAGGGCTTACCTTTGGAGTCACAATCAGGTTTTGAAGCTATTCTATCGGCCATTAGTAGCTTGGCCATGAATGAATGCTATCTCATAGGAGAATTGCCTACAAATATAACACTACAGTCGGATTTGTCAAAGTGGATGGAGAAACTGTTCATGAGCAATGGTTTTCGGGTGAAAATAGTTAGCTATGAAGAGATTGCTGCAAGTTTAGGTGTGGTATTACATTCACCAGTAAACCATGACACGGTTCTCTTTATCGCACAACAGCTCAAGTTTTCAGATGATCAGATGGAGACATGGAGACAAGAATTTCAAAGAGTAGAAGAAGATGGAGATGAACAAGGATTTGAAGAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

333

Amino Acids

37.82

Weight (kDa)

5.74

Isoelectric Point (pI)

60.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 67 - 218 3.3e-22 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 391
AciI CCGC 2 cut(s) 123, 270
AclI AACGTT 1 cut(s) 595
AclWI GGATC 2 cut(s) 532, 545
AcoI YGGCCR 4 cut(s) 117, 452, 693, 708
AcsI RAATTY 2 cut(s) 575, 950
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 886
AgsI TTSAA 7 cut(s) 13, 55, 194, 521, 680, 956, 992
AloI GAACNNNNNNTCC 2 cut(s) 776, 808
Alw21I GWGCWC 2 cut(s) 97, 999
Alw26I GTCTC 3 cut(s) 219, 929, 937
AlwI GGATC 2 cut(s) 532, 545
AoxI GGCC 5 cut(s) 117, 452, 626, 693, 708
ApeKI GCWGC 1 cut(s) 842
ApoI RAATTY 2 cut(s) 575, 950
Asp700I GAANNNNTTC 3 cut(s) 516, 684, 791
AsuHPI GGTGA 3 cut(s) 474, 826, 861
AsuII TTCGAA 1 cut(s) 498
BalI TGGCCA 2 cut(s) 454, 710
BamHI GGATCC 1 cut(s) 537
BanII GRGCYC 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 97, 999
BbvI GCAGC 1 cut(s) 829
BccI CCATC 6 cut(s) 383, 413, 418, 775, 925, 965
BceAI ACGGC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciVI GTATCC 1 cut(s) 421
BclI TGATCA 1 cut(s) 925
BcoDI GTCTC 3 cut(s) 219, 929, 937
BfaI CTAG 2 cut(s) 534, 630
BfmI CTRYAG 1 cut(s) 758
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 421
BisI GCNGC 2 cut(s) 270, 843
BlsI GCNGC 2 cut(s) 271, 844
BmiI GGNNCC 2 cut(s) 24, 539
BmsI GCATC 2 cut(s) 69, 515
BpiI GAAGAC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 896
Bsa29I ATCGAT 1 cut(s) 354
BsaJI CCNNGG 1 cut(s) 570
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse1I ACTGG 1 cut(s) 872
Bse3DI GCAATG 2 cut(s) 648, 808
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 4 cut(s) 84, 309, 429, 786
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMI GCAATG 2 cut(s) 648, 808
BseNI ACTGG 1 cut(s) 872
BseRI GAGGAG 2 cut(s) 87, 90
BseXI GCAGC 1 cut(s) 829
BshFI GGCC 5 cut(s) 119, 454, 628, 695, 710
BshVI ATCGAT 1 cut(s) 354
BsiHKAI GWGCWC 2 cut(s) 97, 999
BsiSI CCGG 2 cut(s) 116, 485
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 3 cut(s) 219, 929, 937
BsmBI CGTCTC 1 cut(s) 219
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 5 cut(s) 119, 454, 628, 695, 710
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 97, 999
Bsp1407I TGTACA 1 cut(s) 106
Bsp143I GATC 4 cut(s) 3, 468, 537, 925
BspACI CCGC 2 cut(s) 123, 270
BspANI GGCC 5 cut(s) 119, 454, 628, 695, 710
BspDI ATCGAT 1 cut(s) 354
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 2 cut(s) 24, 539
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 2 cut(s) 532, 545
BspQI GCTCTTC 1 cut(s) 987
BspT104I TTCGAA 1 cut(s) 498
BsrDI GCAATG 2 cut(s) 648, 808
BsrGI TGTACA 1 cut(s) 106
BsrI ACTGG 1 cut(s) 872
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 4 cut(s) 3, 468, 537, 925
Bst4CI ACNGT 4 cut(s) 335, 762, 792, 889
Bst6I CTCTTC 2 cut(s) 828, 987
BstAUI TGTACA 1 cut(s) 106
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 4 cut(s) 84, 309, 429, 786
BstKTI GATC 4 cut(s) 6, 471, 540, 928
BstMAI GTCTC 3 cut(s) 219, 929, 937
BstMBI GATC 4 cut(s) 3, 468, 537, 925
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 758
BstV1I GCAGC 1 cut(s) 829
BstV2I GAAGAC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 537
BstYI RGATCY 1 cut(s) 537
Bsu15I ATCGAT 1 cut(s) 354
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 5 cut(s) 119, 454, 628, 695, 710
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 4 cut(s) 84, 309, 429, 786
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 153
CciI TCATGA 1 cut(s) 795
ClaI ATCGAT 1 cut(s) 354
CseI GACGC 2 cut(s) 157, 201
Csp6I GTAC 1 cut(s) 107
CviAII CATG 5 cut(s) 314, 712, 796, 881, 939
CviQI GTAC 1 cut(s) 107
DpnI GATC 4 cut(s) 5, 470, 539, 927
DpnII GATC 4 cut(s) 3, 468, 537, 925
EaeI YGGCCR 4 cut(s) 117, 452, 693, 708
Eam1104I CTCTTC 2 cut(s) 828, 987
EarI CTCTTC 2 cut(s) 828, 987
Ecl136II GAGCTC 1 cut(s) 997
Eco24I GRGCYC 1 cut(s) 999
Eco32I GATATC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 997
EcoICRI GAGCTC 1 cut(s) 997
EcoRI GAATTC 1 cut(s) 575
EcoRV GATATC 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 999
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 5 cut(s) 317, 715, 799, 884, 942
FatI CATG 5 cut(s) 313, 711, 795, 880, 938
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 2 cut(s) 270, 843
FokI GGATG 4 cut(s) 91, 296, 436, 793
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 2 cut(s) 270, 843
FspBI CTAG 2 cut(s) 534, 630
GluI GCNGC 2 cut(s) 270, 843
HaeIII GGCC 5 cut(s) 119, 454, 628, 695, 710
HapII CCGG 2 cut(s) 116, 485
HgaI GACGC 2 cut(s) 157, 201
Hin1II CATG 5 cut(s) 317, 715, 799, 884, 942
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 4 cut(s) 85, 190, 622, 662
HpaII CCGG 2 cut(s) 116, 485
HphI GGTGA 3 cut(s) 474, 826, 861
Hpy166II GTNNAC 4 cut(s) 109, 414, 599, 877
Hpy188I TCNGA 7 cut(s) 31, 147, 511, 766, 922, 930, 1001
Hpy188III TCNNGA 2 cut(s) 580, 796
Hpy8I GTNNAC 4 cut(s) 109, 414, 599, 877
Hpy99I CGWCG 2 cut(s) 135, 151
HpyAV CCTTC 2 cut(s) 195, 292
HpyCH4III ACNGT 4 cut(s) 335, 762, 792, 889
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 4 cut(s) 82, 341, 646, 845
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
HpySE526I ACGT 1 cut(s) 595
Hsp92II CATG 5 cut(s) 317, 715, 799, 884, 942
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 4 cut(s) 3, 468, 537, 925
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 2 cut(s) 100, 530
LpnPI CCDG 8 cut(s) 5, 117, 129, 386, 498, 632, 657, 885
Lsp1109I GCAGC 1 cut(s) 829
LweI GCATC 2 cut(s) 69, 515
MaeI CTAG 2 cut(s) 534, 630
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 1 cut(s) 663
MalI GATC 4 cut(s) 5, 470, 539, 927
MboI GATC 4 cut(s) 3, 468, 537, 925
MboII GAAGA 4 cut(s) 39, 845, 977, 980
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 2 cut(s) 97, 999
MlsI TGGCCA 2 cut(s) 454, 710
MluCI AATT 4 cut(s) 448, 575, 737, 950
MluNI TGGCCA 2 cut(s) 454, 710
MlyI GAGTC 2 cut(s) 94, 671
MmeI TCCRAC 2 cut(s) 170, 744
MnlI CCTC 6 cut(s) 25, 27, 108, 111, 258, 294
Mox20I TGGCCA 2 cut(s) 454, 710
MroXI GAANNNNTTC 3 cut(s) 516, 684, 791
MscI TGGCCA 2 cut(s) 454, 710
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 569
Msp20I TGGCCA 2 cut(s) 454, 710
MspI CCGG 2 cut(s) 116, 485
Mva1269I GAATGC 1 cut(s) 725
MwoI GCNNNNNNNGC 1 cut(s) 451
NdeII GATC 4 cut(s) 3, 468, 537, 925
NlaIII CATG 5 cut(s) 317, 715, 799, 884, 942
NlaIV GGNNCC 2 cut(s) 24, 539
NmuCI GTSAC 1 cut(s) 663
NspV TTCGAA 1 cut(s) 498
PagI TCATGA 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 987
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PctI GAATGC 1 cut(s) 725
PdmI GAANNNNTTC 3 cut(s) 516, 684, 791
PfeI GAWTC 2 cut(s) 190, 622
PkrI GCNGC 2 cut(s) 271, 844
PleI GAGTC 2 cut(s) 93, 670
PpsI GAGTC 2 cut(s) 93, 670
Psp124BI GAGCTC 1 cut(s) 999
Psp1406I AACGTT 1 cut(s) 595
PspN4I GGNNCC 2 cut(s) 24, 539
PsuI RGATCY 1 cut(s) 537
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 569
SacI GAGCTC 1 cut(s) 999
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 270, 843
Sau3AI GATC 4 cut(s) 3, 468, 537, 925
SchI GAGTC 2 cut(s) 94, 671
SduI GDGCHC 2 cut(s) 97, 999
SfaNI GCATC 2 cut(s) 69, 515
SfcI CTRYAG 1 cut(s) 758
SfuI TTCGAA 1 cut(s) 498
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 4 cut(s) 448, 575, 737, 950
SsiI CCGC 2 cut(s) 123, 270
SspMI CTAG 2 cut(s) 534, 630
SstI GAGCTC 1 cut(s) 999
TaaI ACNGT 4 cut(s) 335, 762, 792, 889
TaiI ACGT 1 cut(s) 598
TaqI TCGA 8 cut(s) 139, 188, 238, 258, 354, 433, 498, 579
TasI AATT 4 cut(s) 448, 575, 737, 950
TatI WGTACW 1 cut(s) 106
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 2 cut(s) 190, 622
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 1 cut(s) 842
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 9 cut(s) 39, 244, 330, 340, 728, 732, 784, 846, 993
TspGWI ACGGA 3 cut(s) 15, 91, 119
XapI RAATTY 2 cut(s) 575, 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmnI GAANNNNTTC 3 cut(s) 516, 684, 791
XspI CTAG 2 cut(s) 534, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.