pycom11g27040

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011175
Physical Location & Seq
Reverse (-)
29439323 .. 29442478
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g27040.6

Sequence Viewer

Length: 1014 bp
ATGGACTGTTTAGCGGGCGTTGCACATGCCCCGGCTGCTGCTATCTTGCGCCGATCGGTCCTCTACACCACTTCAAGAAGGGGGGATAGAAATGGCGGGTTGAAGTTGCGAGCCAAAATCGTCGAGGAAAACGACCCATTACTTCAATCTGCCATTGATTCGGCTTCTCTTCGTTTCCGGGAGACCCATCGACCAAAGCCTCTGTTTGTTGATCCGTATGCTGGTTGCTTTGTTCCTCCTAATGCTCAGATGGATATGATGCAATGCTCACATCATTATTGCGTTGCTACTAAATTCATGGACGACAAGTTGCTTCGAACAGTAAACCATATCGATGGATTAAAGCAGGTTGTTTTGCTAACCGATGGAATGGATACCCGCCCATATAGGCTTAGTTGGCCAACTTCAACCATTATATTCAATGTTTCTCCGGAACTGGTGTTCAAAAGAGCAGCCGAGAAGCTTCAAGGTGTTGGGGCTAAGATTCCTAGAAGTTGCTTATCCCTTCATGTTCCTTTGGAATCCTCCGACATACGGAAAAGTCTGTGTGCTAAAGGGTTTAATGACAATCAACCTAGCATTTGGGCCATGCAGGGGCTTCCTTTGATGACATTAGCAAATTTTGAAGATATTTTGCTCACTGTAAGTGGTTTGGCGATGAACGGATGTTTTTTCTTGGGAGAATTACCTGCTTGGTTGGCAGAAACTGAAATAGGGACCAAGACTAGTGCAAGGACATGGTTGGAGAAACTATTCATGAACAATGGATTTCGGGTGGACATGATTTGTTTTGATGAAGTTGCAAGGAGTTTAGGCAAGGAATTAGCACCGGGAAGGTATAAGAATGTACTATTTTCAGCAGAACAACTTCGGTTTTCTGATGATCAGATCTCTGCCTTCGCTGCTGAAATACTGAACACTTTTCATGATCATTCTGATGCTTGGCAAATTCTTTGTGATCTTGTTGCATCACAGAGGCCCACGATTTCTCCAGTTCCATTTGTGAGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

338

Amino Acids

37.51

Weight (kDa)

7.07

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 337, 697
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 3 cut(s) 14, 96, 379
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 3 cut(s) 293, 619, 948
AfaI GTAC 1 cut(s) 849
AfeI AGCGCT 1 cut(s) 1009
AfiI CCNNNNNNNGG 3 cut(s) 221, 534, 594
AgsI TTSAA 8 cut(s) 75, 103, 146, 408, 421, 445, 467, 626
AhlI ACTAGT 1 cut(s) 725
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 1 cut(s) 463
AluI AGCT 1 cut(s) 463
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 707
Aor13HI TCCGGA 1 cut(s) 430
Aor51HI AGCGCT 1 cut(s) 1009
AoxI GGCC 3 cut(s) 398, 585, 977
ApeKI GCWGC 4 cut(s) 35, 38, 452, 902
ApoI RAATTY 3 cut(s) 293, 619, 948
Asp700I GAANNNNTTC 2 cut(s) 752, 867
AspLEI GCGC 2 cut(s) 51, 1010
AspS9I GGNCC 4 cut(s) 58, 585, 717, 978
AsuC2I CCSGG 3 cut(s) 32, 179, 831
AsuII TTCGAA 1 cut(s) 316
AvaII GGWCC 2 cut(s) 58, 717
BalI TGGCCA 1 cut(s) 400
BbvI GCAGC 4 cut(s) 22, 25, 464, 889
BccI CCATC 4 cut(s) 195, 244, 329, 359
BciVI GTATCC 1 cut(s) 367
BclI TGATCA 2 cut(s) 883, 928
BcnI CCSGG 3 cut(s) 32, 179, 831
BcoDI GTCTC 1 cut(s) 176
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 3 cut(s) 489, 576, 726
BfoI RGCGCY 1 cut(s) 1011
BfuAI ACCTGC 2 cut(s) 337, 697
BfuI GTATCC 1 cut(s) 367
BglII AGATCT 1 cut(s) 888
BisI GCNGC 4 cut(s) 36, 39, 453, 903
BlsI GCNGC 4 cut(s) 37, 40, 454, 904
Bme1390I CCNGG 3 cut(s) 32, 179, 831
Bme18I GGWCC 2 cut(s) 58, 717
BmgT120I GGNCC 4 cut(s) 58, 585, 717, 978
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 3 cut(s) 32, 179, 831
BmsI GCATC 3 cut(s) 249, 928, 977
BpmI CTGGAG 1 cut(s) 975
Bpu14I TTCGAA 1 cut(s) 316
BpuMI CCSGG 3 cut(s) 32, 179, 831
Bsa29I ATCGAT 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 973, 1003
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 534, 594
Bse1I ACTGG 2 cut(s) 441, 992
Bse3DI GCAATG 1 cut(s) 269
BseAI TCCGGA 1 cut(s) 430
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 3 cut(s) 221, 534, 594
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 441, 992
BseXI GCAGC 4 cut(s) 22, 25, 464, 889
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 400, 587, 979
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 56
BsiSI CCGG 4 cut(s) 32, 178, 431, 830
BslFI GGGAC 1 cut(s) 730
BslI CCNNNNNNNGG 3 cut(s) 221, 534, 594
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 730
BsnI GGCC 3 cut(s) 400, 587, 979
Bso31I GGTCTC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 316
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 6 cut(s) 53, 211, 883, 888, 928, 958
BspACI CCGC 3 cut(s) 14, 96, 379
BspANI GGCC 3 cut(s) 400, 587, 979
BspCNI CTCAG 1 cut(s) 259
BspDI ATCGAT 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 430
BspHI TCATGA 2 cut(s) 756, 925
BspLI GGNNCC 1 cut(s) 718
BspMI ACCTGC 2 cut(s) 337, 697
BspPI GGATC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 316
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 269
BsrI ACTGG 2 cut(s) 441, 992
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 6 cut(s) 53, 211, 883, 888, 928, 958
Bst4CI ACNGT 3 cut(s) 8, 322, 643
Bst6I CTCTTC 1 cut(s) 174
BstBI TTCGAA 1 cut(s) 316
BstC8I GCNNGC 2 cut(s) 16, 111
BstDEI CTNAG 3 cut(s) 246, 392, 480
BstF5I GGATG 1 cut(s) 671
BstH2I RGCGCY 1 cut(s) 1011
BstHHI GCGC 2 cut(s) 51, 1010
BstKTI GATC 6 cut(s) 56, 214, 886, 891, 931, 961
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 6 cut(s) 53, 211, 883, 888, 928, 958
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 5 cut(s) 20, 35, 397, 698, 902
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 3 cut(s) 30, 177, 829
BstV1I GCAGC 4 cut(s) 22, 25, 464, 889
BstX2I RGATCY 1 cut(s) 888
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 1 cut(s) 888
Bsu15I ATCGAT 1 cut(s) 333
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 400, 587, 979
BsuTUI ATCGAT 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 671
BtsCI GGATG 1 cut(s) 671
BtsIMutI CAGTG 1 cut(s) 639
BveI ACCTGC 2 cut(s) 337, 697
Cac8I GCNNGC 2 cut(s) 16, 111
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 2 cut(s) 756, 925
CfoI GCGC 2 cut(s) 51, 1010
Cfr13I GGNCC 4 cut(s) 58, 585, 717, 978
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 1 cut(s) 848
CviAII CATG 8 cut(s) 26, 298, 509, 589, 738, 757, 781, 926
CviQI GTAC 1 cut(s) 848
DdeI CTNAG 3 cut(s) 246, 392, 480
DpnI GATC 6 cut(s) 55, 213, 885, 890, 930, 960
DpnII GATC 6 cut(s) 53, 211, 883, 888, 928, 958
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 58, 717
Eco47III AGCGCT 1 cut(s) 1009
FaeI CATG 8 cut(s) 29, 301, 512, 592, 741, 760, 784, 929
FaqI GGGAC 1 cut(s) 730
FatI CATG 8 cut(s) 25, 297, 508, 588, 737, 756, 780, 925
FauI CCCGC 3 cut(s) 7, 89, 386
FbaI TGATCA 2 cut(s) 883, 928
Fnu4HI GCNGC 4 cut(s) 36, 39, 453, 903
FokI GGATG 1 cut(s) 678
Fsp4HI GCNGC 4 cut(s) 36, 39, 453, 903
FspBI CTAG 3 cut(s) 489, 576, 726
GlaI GCGC 2 cut(s) 50, 1009
GluI GCNGC 4 cut(s) 36, 39, 453, 903
GsuI CTGGAG 1 cut(s) 975
HaeII RGCGCY 1 cut(s) 1011
HaeIII GGCC 3 cut(s) 400, 587, 979
HapII CCGG 4 cut(s) 32, 178, 431, 830
HhaI GCGC 2 cut(s) 51, 1010
Hin1II CATG 8 cut(s) 29, 301, 512, 592, 741, 760, 784, 929
Hin6I GCGC 2 cut(s) 49, 1008
HinP1I GCGC 2 cut(s) 49, 1008
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 3 cut(s) 158, 484, 521
HpaII CCGG 4 cut(s) 32, 178, 431, 830
Hpy166II GTNNAC 2 cut(s) 325, 778
Hpy188I TCNGA 5 cut(s) 249, 529, 880, 888, 937
Hpy188III TCNNGA 4 cut(s) 75, 431, 757, 926
Hpy8I GTNNAC 2 cut(s) 325, 778
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 72, 515, 828, 907
HpyCH4III ACNGT 3 cut(s) 8, 322, 643
HpyCH4V TGCA 6 cut(s) 23, 262, 592, 731, 803, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 35, 397, 698, 902
HpyF3I CTNAG 3 cut(s) 246, 392, 480
Hsp92II CATG 8 cut(s) 29, 301, 512, 592, 741, 760, 784, 929
HspAI GCGC 2 cut(s) 49, 1008
Kpn2I TCCGGA 1 cut(s) 430
Ksp22I TGATCA 2 cut(s) 883, 928
Kzo9I GATC 6 cut(s) 53, 211, 883, 888, 928, 958
Lsp1109I GCAGC 4 cut(s) 22, 25, 464, 889
LweI GCATC 3 cut(s) 249, 928, 977
MaeI CTAG 3 cut(s) 489, 576, 726
MalI GATC 6 cut(s) 55, 213, 885, 890, 930, 960
MboI GATC 6 cut(s) 53, 211, 883, 888, 928, 958
MboII GAAGA 2 cut(s) 161, 638
MflI RGATCY 1 cut(s) 888
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 5 cut(s) 293, 619, 683, 821, 948
MluNI TGGCCA 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 552, 723
MnlI CCTC 6 cut(s) 71, 118, 210, 246, 535, 969
Mox20I TGGCCA 1 cut(s) 400
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 2 cut(s) 752, 867
MscI TGGCCA 1 cut(s) 400
MseI TTAA 2 cut(s) 341, 561
MslI CAYNNNNRTG 2 cut(s) 333, 936
Msp20I TGGCCA 1 cut(s) 400
MspI CCGG 4 cut(s) 32, 178, 431, 830
MspR9I CCNGG 3 cut(s) 32, 179, 831
MwoI GCNNNNNNNGC 5 cut(s) 20, 35, 397, 698, 902
NciI CCSGG 3 cut(s) 32, 179, 831
NdeII GATC 6 cut(s) 53, 211, 883, 888, 928, 958
NlaIII CATG 8 cut(s) 29, 301, 512, 592, 741, 760, 784, 929
NlaIV GGNNCC 1 cut(s) 718
NmeAIII GCCGAG 1 cut(s) 481
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 1 cut(s) 316
PagI TCATGA 2 cut(s) 756, 925
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 752, 867
PfeI GAWTC 3 cut(s) 158, 484, 521
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 4 cut(s) 37, 40, 454, 904
Ple19I CGATCG 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 4 cut(s) 58, 585, 717, 978
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 1 cut(s) 888
PvuI CGATCG 1 cut(s) 56
RsaI GTAC 1 cut(s) 849
RsaNI GTAC 1 cut(s) 848
RseI CAYNNNNRTG 2 cut(s) 333, 936
SaqAI TTAA 2 cut(s) 341, 561
SatI GCNGC 4 cut(s) 36, 39, 453, 903
Sau3AI GATC 6 cut(s) 53, 211, 883, 888, 928, 958
Sau96I GGNCC 4 cut(s) 58, 585, 717, 978
ScrFI CCNGG 3 cut(s) 32, 179, 831
SetI ASST 6 cut(s) 351, 465, 472, 577, 691, 839
SfaNI GCATC 3 cut(s) 249, 928, 977
SfuI TTCGAA 1 cut(s) 316
SinI GGWCC 2 cut(s) 58, 717
SmiMI CAYNNNNRTG 2 cut(s) 333, 936
SpeI ACTAGT 1 cut(s) 725
Sse9I AATT 5 cut(s) 293, 619, 683, 821, 948
SsiI CCGC 3 cut(s) 14, 96, 379
SspMI CTAG 3 cut(s) 489, 576, 726
StyD4I CCNGG 3 cut(s) 30, 177, 829
TaaI ACNGT 3 cut(s) 8, 322, 643
TaqI TCGA 4 cut(s) 123, 190, 316, 333
TaqII GACCGA 1 cut(s) 46
TasI AATT 5 cut(s) 293, 619, 683, 821, 948
TatI WGTACW 1 cut(s) 847
TfiI GAWTC 3 cut(s) 158, 484, 521
Tru1I TTAA 2 cut(s) 341, 561
Tru9I TTAA 2 cut(s) 341, 561
TscAI CASTG 1 cut(s) 646
TseI GCWGC 4 cut(s) 35, 38, 452, 902
TspDTI ATGAA 7 cut(s) 286, 497, 674, 745, 773, 810, 914
TspGWI ACGGA 3 cut(s) 204, 550, 678
TspRI CASTG 1 cut(s) 646
VpaK11BI GGWCC 2 cut(s) 58, 717
XapI RAATTY 3 cut(s) 293, 619, 948
XceI RCATGY 1 cut(s) 29
XmnI GAANNNNTTC 2 cut(s) 752, 867
XspI CTAG 3 cut(s) 489, 576, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.