Rorug06G0241200

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011175
Physical Location & Seq
Reverse (-)
38448158 .. 38448973
816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0241200.1

Sequence Viewer

Length: 816 bp
ATGGCTTCCCACACTCGCTCTAACAGCTTCCCCTCTAGGCCACACCCTATCATTGAAGAAGCTAATGAACAATTGTGCAGATTGAGGTCTTCTGCAGCCACCTCAACATCTTCATCATCAATAAGCCACAAACTAAGTGGTCTAGAAAACTTGCATGATTGTGTTGAAAGGCTGCTTCAGTTGCCCCTCAATCAACAGGCTTTAGCACAAGAGAAACATCAGAAATGCACTAATGAGCTATTAGATGGTTCCCTCAGACTCTTGGATGTTTGTAGCATTGCCAAGGAAGCCCTGCAGCAAACCAAGGAATGCTTACAGGATCTTCAATCTATCATAAGAAGACGAGGATGCGAATCTGGAGTACTTACAAGTGAGGTTAGGAGATACTTGGTCTCCAGGAAGATGGTGAAAAAGACAATCCAAAAGGCTATGGTCAATACCAGATCCTCTTTCTCTTCACTCAACAAGGAGACTGAATCTATTTCCATTGTTAACATGTTAAGAAATGTAGAAGCAATTACTCTTACAGTGTTTGAATCACTGTTGTCCTTCATCTTGGGGCCAAAGGCATCAAGCAGCTGGTCATTGGTATCAAAGATGATGCAGTCAAAAAGAGTAGCTTGTGAGGAAGAAGCAGAAATAAATGAATTCGCAGAGGTGGATGCTGCATTGAAGTCACTCAAGAGTGCAGACAGTGTACAGAACCAGCTTAACAATCTGGAGTCATGCATCCAAGATCAAGAACAAGGACTCGAGAGCCTATTTAGGCAATTGATCAAGACTAGAGTCTCCCTTCTCAACATCCTCAACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

271

Amino Acids

30.33

Weight (kDa)

8.11

Isoelectric Point (pI)

69.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BPS1 PF03087 44 - 268 1e-78 Protein BPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 327, 438
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 2 cut(s) 363, 699
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 5 cut(s) 56, 167, 326, 536, 673
AjnI CCWGG 1 cut(s) 395
AluBI AGCT 6 cut(s) 27, 62, 238, 579, 620, 709
AluI AGCT 6 cut(s) 27, 62, 238, 579, 620, 709
Alw26I GTCTC 3 cut(s) 397, 464, 793
AlwI GGATC 2 cut(s) 327, 438
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 2 cut(s) 38, 560
ApeKI GCWGC 5 cut(s) 95, 172, 295, 576, 665
ApoI RAATTY 1 cut(s) 647
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 752
BbsI GAAGAC 2 cut(s) 81, 346
BbvI GCAGC 5 cut(s) 107, 159, 307, 588, 652
BccI CCATC 2 cut(s) 239, 397
BciT130I CCWGG 1 cut(s) 397
BclI TGATCA 1 cut(s) 774
BcoDI GTCTC 3 cut(s) 397, 464, 793
BfaI CTAG 4 cut(s) 36, 143, 783, 814
BfmI CTRYAG 2 cut(s) 93, 293
BisI GCNGC 5 cut(s) 96, 173, 296, 577, 666
BlsI GCNGC 5 cut(s) 97, 174, 297, 578, 667
BmcAI AGTACT 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 2 cut(s) 250, 561
BmrFI CCNGG 1 cut(s) 397
BmsI GCATC 5 cut(s) 338, 578, 591, 652, 738
BoxI GACNNNNGTC 1 cut(s) 785
BpiI GAAGAC 2 cut(s) 81, 346
BpmI CTGGAG 3 cut(s) 378, 379, 740
BpuEI CTTGAG 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 352
BsaI GGTCTC 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 282, 303
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 352
BseBI CCWGG 1 cut(s) 397
BseDI CCNNGG 2 cut(s) 282, 303
BseGI GGATG 5 cut(s) 271, 353, 667, 729, 801
BseJI GATNNNNATC 1 cut(s) 352
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 1 cut(s) 268
BseXI GCAGC 5 cut(s) 107, 159, 307, 588, 652
BsgI GTGCAG 2 cut(s) 97, 708
BshFI GGCC 2 cut(s) 40, 562
BsiHKCI CYCGRG 1 cut(s) 752
BsmAI GTCTC 3 cut(s) 397, 464, 793
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 2 cut(s) 40, 562
Bso31I GGTCTC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 752
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 4 cut(s) 319, 443, 736, 774
BspANI GGCC 2 cut(s) 40, 562
BspCNI CTCAG 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 250, 561
BspMAI CTGCAG 2 cut(s) 97, 297
BspPI GGATC 2 cut(s) 327, 438
BspTNI GGTCTC 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 276
BsrGI TGTACA 1 cut(s) 697
BssECI CCNNGG 2 cut(s) 282, 303
BssMI GATC 4 cut(s) 319, 443, 736, 774
BssT1I CCWWGG 2 cut(s) 282, 303
Bst2UI CCWGG 1 cut(s) 397
Bst4CI ACNGT 3 cut(s) 529, 543, 695
Bst6I CTCTTC 1 cut(s) 460
BstAUI TGTACA 1 cut(s) 697
BstDEI CTNAG 2 cut(s) 134, 254
BstF5I GGATG 5 cut(s) 271, 353, 667, 729, 801
BstKTI GATC 4 cut(s) 322, 446, 739, 777
BstMAI GTCTC 3 cut(s) 397, 464, 793
BstMBI GATC 4 cut(s) 319, 443, 736, 774
BstMWI GCNNNNNNNGC 3 cut(s) 24, 181, 287
BstNI CCWGG 1 cut(s) 397
BstNSI RCATGY 1 cut(s) 499
BstPAI GACNNNNGTC 1 cut(s) 785
BstSCI CCNGG 1 cut(s) 395
BstSFI CTRYAG 2 cut(s) 93, 293
BstV1I GCAGC 5 cut(s) 107, 159, 307, 588, 652
BstV2I GAAGAC 2 cut(s) 81, 346
BstX2I RGATCY 2 cut(s) 319, 443
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 2 cut(s) 319, 443
BsuRI GGCC 2 cut(s) 40, 562
BtsCI GGATG 5 cut(s) 271, 353, 667, 729, 801
BtsIMutI CAGTG 3 cut(s) 534, 539, 700
Cfr13I GGNCC 1 cut(s) 560
Csp6I GTAC 2 cut(s) 362, 698
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 3 cut(s) 155, 496, 726
CviQI GTAC 2 cut(s) 362, 698
DdeI CTNAG 2 cut(s) 134, 254
DpnI GATC 4 cut(s) 321, 445, 738, 776
DpnII GATC 4 cut(s) 319, 443, 736, 774
Eam1104I CTCTTC 1 cut(s) 460
EarI CTCTTC 1 cut(s) 460
Eco130I CCWWGG 2 cut(s) 282, 303
Eco31I GGTCTC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 752
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 395
EcoT14I CCWWGG 2 cut(s) 282, 303
EcoT22I ATGCAT 1 cut(s) 731
ErhI CCWWGG 2 cut(s) 282, 303
FaeI CATG 3 cut(s) 158, 499, 729
FaiI YATR 5 cut(s) 156, 335, 431, 497, 727
FalI AAGNNNNNCTT 4 cut(s) 296, 328, 604, 636
FatI CATG 3 cut(s) 154, 495, 725
FbaI TGATCA 1 cut(s) 774
Fnu4HI GCNGC 5 cut(s) 96, 173, 296, 577, 666
FokI GGATG 5 cut(s) 278, 360, 674, 716, 788
Fsp4HI GCNGC 5 cut(s) 96, 173, 296, 577, 666
FspBI CTAG 4 cut(s) 36, 143, 783, 814
GluI GCNGC 5 cut(s) 96, 173, 296, 577, 666
GsuI CTGGAG 3 cut(s) 378, 379, 740
HaeIII GGCC 2 cut(s) 40, 562
Hin1II CATG 3 cut(s) 158, 499, 729
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 7 cut(s) 258, 353, 476, 536, 722, 750, 786
HpaI GTTAAC 1 cut(s) 493
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 2 cut(s) 493, 698
Hpy188I TCNGA 2 cut(s) 222, 257
Hpy188III TCNNGA 7 cut(s) 143, 357, 682, 719, 740, 754, 778
Hpy8I GTNNAC 2 cut(s) 493, 698
HpyAV CCTTC 2 cut(s) 559, 803
HpyCH4III ACNGT 3 cut(s) 529, 543, 695
HpyCH4V TGCA 9 cut(s) 78, 95, 154, 228, 295, 604, 668, 689, 729
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 181, 287
HpyF3I CTNAG 2 cut(s) 134, 254
Hsp92II CATG 3 cut(s) 158, 499, 729
Ksp22I TGATCA 1 cut(s) 774
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 4 cut(s) 319, 443, 736, 774
Lsp1109I GCAGC 5 cut(s) 107, 159, 307, 588, 652
LweI GCATC 5 cut(s) 338, 578, 591, 652, 738
MaeI CTAG 4 cut(s) 36, 143, 783, 814
MaeIII GTNAC 1 cut(s) 675
MalI GATC 4 cut(s) 321, 445, 738, 776
MboI GATC 4 cut(s) 319, 443, 736, 774
MboII GAAGA 8 cut(s) 68, 81, 102, 314, 351, 412, 447, 641
MfeI CAATTG 2 cut(s) 71, 770
MflI RGATCY 2 cut(s) 319, 443
MluCI AATT 4 cut(s) 71, 516, 647, 770
MlyI GAGTC 4 cut(s) 252, 731, 744, 795
Mph1103I ATGCAT 1 cut(s) 731
MseI TTAA 3 cut(s) 492, 500, 711
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 579
MspR9I CCNGG 1 cut(s) 397
MunI CAATTG 2 cut(s) 71, 770
Mva1269I GAATGC 1 cut(s) 314
MvaI CCWGG 1 cut(s) 397
MwoI GCNNNNNNNGC 3 cut(s) 24, 181, 287
NdeII GATC 4 cut(s) 319, 443, 736, 774
NlaIII CATG 3 cut(s) 158, 499, 729
NlaIV GGNNCC 2 cut(s) 250, 561
NmuCI GTSAC 1 cut(s) 675
NsiI ATGCAT 1 cut(s) 731
NspI RCATGY 1 cut(s) 499
PaeR7I CTCGAG 1 cut(s) 752
PciI ACATGT 1 cut(s) 495
PctI GAATGC 1 cut(s) 314
PfeI GAWTC 3 cut(s) 353, 476, 536
PfoI TCCNGGA 1 cut(s) 395
PkrI GCNGC 5 cut(s) 97, 174, 297, 578, 667
PleI GAGTC 4 cut(s) 252, 730, 744, 794
PpsI GAGTC 4 cut(s) 252, 730, 744, 794
PscI ACATGT 1 cut(s) 495
PshAI GACNNNNGTC 1 cut(s) 785
Psp6I CCWGG 1 cut(s) 395
PspGI CCWGG 1 cut(s) 395
PspN4I GGNNCC 2 cut(s) 250, 561
PspPI GGNCC 1 cut(s) 560
PstI CTGCAG 2 cut(s) 97, 297
PsuI RGATCY 2 cut(s) 319, 443
PvuII CAGCTG 1 cut(s) 579
RsaI GTAC 2 cut(s) 363, 699
RsaNI GTAC 2 cut(s) 362, 698
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 492, 500, 711
SatI GCNGC 5 cut(s) 96, 173, 296, 577, 666
Sau3AI GATC 4 cut(s) 319, 443, 736, 774
Sau96I GGNCC 1 cut(s) 560
ScaI AGTACT 1 cut(s) 363
SchI GAGTC 4 cut(s) 252, 731, 744, 795
ScrFI CCNGG 1 cut(s) 397
SfaNI GCATC 5 cut(s) 338, 578, 591, 652, 738
SfcI CTRYAG 2 cut(s) 93, 293
Sfr274I CTCGAG 1 cut(s) 752
SlaI CTCGAG 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 2 cut(s) 680, 752
SmoI CTYRAG 2 cut(s) 680, 752
Sse9I AATT 4 cut(s) 71, 516, 647, 770
SspMI CTAG 4 cut(s) 36, 143, 783, 814
StyD4I CCNGG 1 cut(s) 395
StyI CCWWGG 2 cut(s) 282, 303
TaaI ACNGT 3 cut(s) 529, 543, 695
TaqI TCGA 1 cut(s) 753
TasI AATT 4 cut(s) 71, 516, 647, 770
TatI WGTACW 2 cut(s) 361, 697
TfiI GAWTC 3 cut(s) 353, 476, 536
Tru1I TTAA 3 cut(s) 492, 500, 711
Tru9I TTAA 3 cut(s) 492, 500, 711
TscAI CASTG 3 cut(s) 534, 546, 700
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 5 cut(s) 95, 172, 295, 576, 665
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 4 cut(s) 81, 102, 541, 660
TspRI CASTG 3 cut(s) 534, 546, 700
XapI RAATTY 1 cut(s) 647
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 1 cut(s) 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 752
XspI CTAG 4 cut(s) 36, 143, 783, 814
ZrmI AGTACT 1 cut(s) 363
Zsp2I ATGCAT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.