MD03G1288800.v1.1

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011175
Physical Location & Seq
Forward (+)
36731968 .. 36734893
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1288800.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGACTGTTTAGCGGGTTTTTCACATGCTCCGGCGACTGCTATCTTGCGCCGACCAGTGCTCTACAACGCTTTAAGAAGAACAGGTAGAAATGGGGGGTTGAAACTGCGAGCCAAAATCGTCGAGGAAAACGACCCAATACTGAAATCCGCCATTGATTCAGCTTCCCTTCGTTTCCGAGAGACCCATCGACCAGAGCCTCTGTTTGTTGATCCGTATGCTGGTTGCTTTGTTCCTCCTGATGTTCAGATGGATATGAAGCAATGCTCACATCATTATTGCGTTGCTACGAAATTCATGGACGATAAGTTGCTTCGAACAGTAAACCATATTGATGGACTTAAGCAGGTTGTTTTGCTAACCGATGGAATGGATACCCGTCCCTACAGGCTCAGTTGGCCAACTTCAACCATTATTTTTAATGTCTCTCCGGAACGGGTGTTCAAAACAGCAACTGAGAAGCTTCGAGGTGTTGGGGCTAAGATTCCAAGAAGCTGCTTATCCCTTCATGTTCCTTTGGAATCCTCCAACATACAGGAAAGTCTGCGTGCTAAAGGGTTTAACGGCAACCTGCCTAGTATTTGGGCCATGCAGGGGCTGCCTTTGATGACATTAGCAAGTTTTGAAGACATTTTGCTTACTGTAAGTGGTTCAGCGATGAACGGATGTTTTTTCATGGGAGAATTGCCGGCTTGGTTGGCAGAAACTGAAGTAGGGACCAAGACTAGTGCAAGGACATGGTTGGAGAAACTATTCATGAACAACGGCTTTCGGGTGGACATGGTTTGCTATGAGGAAGTTGCAAGGAGTTTAGGCAAGGATTTAGCACCGGGAAGCTATAAGAACATACTATTTTCTGCAGAACAACTTCGGTTTTCTGATGATCAGATGGAAACTTGGAGGAAGGAATTCCAAAGAACCGAGGAAGAAGGGGATGAGGAAGGTTTCGAAGAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

320

Amino Acids

35.97

Weight (kDa)

5.95

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 52 - 200 3.6e-22 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 337, 579
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 2 cut(s) 14, 150
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 2 cut(s) 293, 908
AcuI CTGAAG 1 cut(s) 729
AfiI CCNNNNNNNGG 2 cut(s) 221, 594
AflII CTTAAG 1 cut(s) 341
AgsI TTSAA 4 cut(s) 103, 408, 445, 626
AhlI ACTAGT 1 cut(s) 725
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 5 cut(s) 164, 463, 495, 837, 955
AluI AGCT 5 cut(s) 164, 463, 495, 837, 955
Alw21I GWGCWC 2 cut(s) 63, 957
Alw26I GTCTC 2 cut(s) 176, 430
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 3 cut(s) 455, 598, 707
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 2 cut(s) 398, 585
ApeKI GCWGC 2 cut(s) 495, 598
ApoI RAATTY 2 cut(s) 293, 908
Asp700I GAANNNNTTC 3 cut(s) 752, 867, 908
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 585, 717
AsuC2I CCSGG 1 cut(s) 831
AsuII TTCGAA 2 cut(s) 316, 948
AvaII GGWCC 1 cut(s) 717
BalI TGGCCA 1 cut(s) 400
BanII GRGCYC 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 2 cut(s) 63, 957
BbvI GCAGC 2 cut(s) 482, 585
BccI CCATC 5 cut(s) 195, 244, 329, 359, 883
BceAI ACGGC 2 cut(s) 580, 781
BciVI GTATCC 1 cut(s) 367
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 1 cut(s) 831
BcoDI GTCTC 2 cut(s) 176, 430
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 2 cut(s) 576, 726
BfmI CTRYAG 2 cut(s) 385, 858
BfrI CTTAAG 1 cut(s) 341
BfuAI ACCTGC 2 cut(s) 337, 579
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 2 cut(s) 496, 599
BlsI GCNGC 2 cut(s) 497, 600
Bme1390I CCNGG 1 cut(s) 831
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 2 cut(s) 585, 717
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 1 cut(s) 831
BpiI GAAGAC 1 cut(s) 633
Bpu14I TTCGAA 2 cut(s) 316, 948
BpuMI CCSGG 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 921
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 594
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 269
BseAI TCCGGA 1 cut(s) 430
BseDI CCNNGG 1 cut(s) 921
BseGI GGATG 2 cut(s) 671, 940
BseLI CCNNNNNNNGG 2 cut(s) 221, 594
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 406, 447
BseNI ACTGG 1 cut(s) 56
BseXI GCAGC 2 cut(s) 482, 585
BshFI GGCC 2 cut(s) 400, 587
BsiHKAI GWGCWC 2 cut(s) 63, 957
BsiSI CCGG 4 cut(s) 32, 431, 689, 830
BslFI GGGAC 2 cut(s) 366, 730
BslI CCNNNNNNNGG 2 cut(s) 221, 594
BsmAI GTCTC 2 cut(s) 176, 430
BsmFI GGGAC 2 cut(s) 366, 730
BsnI GGCC 2 cut(s) 400, 587
Bso31I GGTCTC 1 cut(s) 176
Bsp119I TTCGAA 2 cut(s) 316, 948
Bsp1286I GDGCHC 2 cut(s) 63, 957
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 2 cut(s) 211, 883
BspACI CCGC 2 cut(s) 14, 150
BspANI GGCC 2 cut(s) 400, 587
BspCNI CTCAG 2 cut(s) 405, 448
BspEI TCCGGA 1 cut(s) 430
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 718
BspMAI CTGCAG 1 cut(s) 862
BspMI ACCTGC 2 cut(s) 337, 579
BspPI GGATC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 945
BspT104I TTCGAA 2 cut(s) 316, 948
BspTI CTTAAG 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 1 cut(s) 56
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 1 cut(s) 921
BssMI GATC 2 cut(s) 211, 883
Bst4CI ACNGT 3 cut(s) 8, 322, 643
Bst6I CTCTTC 1 cut(s) 945
BstAFI CTTAAG 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 598
BstBI TTCGAA 2 cut(s) 316, 948
BstC8I GCNNGC 3 cut(s) 111, 549, 690
BstDEI CTNAG 3 cut(s) 392, 456, 480
BstF5I GGATG 2 cut(s) 671, 940
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 2 cut(s) 214, 886
BstMAI GTCTC 2 cut(s) 176, 430
BstMBI GATC 2 cut(s) 211, 883
BstMWI GCNNNNNNNGC 3 cut(s) 397, 598, 698
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 829
BstSFI CTRYAG 2 cut(s) 385, 858
BstV1I GCAGC 2 cut(s) 482, 585
BstV2I GAAGAC 1 cut(s) 633
BstXI CCANNNNNNTGG 1 cut(s) 335
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 400, 587
BtgZI GCGATG 1 cut(s) 671
BtsCI GGATG 2 cut(s) 671, 940
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 2 cut(s) 337, 579
Cac8I GCNNGC 3 cut(s) 111, 549, 690
CaiI CAGNNNCTG 3 cut(s) 455, 598, 707
CciI TCATGA 1 cut(s) 756
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 2 cut(s) 585, 717
CviAII CATG 8 cut(s) 26, 298, 509, 589, 676, 738, 757, 781
DdeI CTNAG 3 cut(s) 392, 456, 480
DpnI GATC 2 cut(s) 213, 885
DpnII GATC 2 cut(s) 211, 883
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 945
EarI CTCTTC 1 cut(s) 945
EciI GGCGGA 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 955
Eco24I GRGCYC 1 cut(s) 957
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 717
Eco53kI GAGCTC 1 cut(s) 955
Eco57I CTGAAG 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 955
EcoRI GAATTC 1 cut(s) 908
EcoT38I GRGCYC 1 cut(s) 957
FaeI CATG 8 cut(s) 29, 301, 512, 592, 679, 741, 760, 784
FaqI GGGAC 2 cut(s) 366, 730
FatI CATG 8 cut(s) 25, 297, 508, 588, 675, 737, 756, 780
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 2 cut(s) 496, 599
FokI GGATG 2 cut(s) 678, 947
FriOI GRGCYC 1 cut(s) 957
Fsp4HI GCNGC 2 cut(s) 496, 599
FspBI CTAG 2 cut(s) 576, 726
GlaI GCGC 1 cut(s) 50
GluI GCNGC 2 cut(s) 496, 599
HaeIII GGCC 2 cut(s) 400, 587
HapII CCGG 4 cut(s) 32, 431, 689, 830
HhaI GCGC 1 cut(s) 51
Hin1II CATG 8 cut(s) 29, 301, 512, 592, 679, 741, 760, 784
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 3 cut(s) 158, 484, 521
HpaII CCGG 4 cut(s) 32, 431, 689, 830
Hpy166II GTNNAC 2 cut(s) 325, 778
Hpy188I TCNGA 4 cut(s) 179, 249, 880, 888
Hpy188III TCNNGA 3 cut(s) 239, 431, 757
Hpy8I GTNNAC 2 cut(s) 325, 778
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 5 cut(s) 179, 515, 898, 923, 935
HpyCH4III ACNGT 3 cut(s) 8, 322, 643
HpyCH4V TGCA 4 cut(s) 592, 731, 803, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 397, 598, 698
HpyF3I CTNAG 3 cut(s) 392, 456, 480
Hsp92II CATG 8 cut(s) 29, 301, 512, 592, 679, 741, 760, 784
HspAI GCGC 1 cut(s) 49
Kpn2I TCCGGA 1 cut(s) 430
KroI GCCGGC 1 cut(s) 688
KroNI GCCGGC 1 cut(s) 690
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 2 cut(s) 211, 883
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 2 cut(s) 482, 585
MaeI CTAG 2 cut(s) 576, 726
MalI GATC 2 cut(s) 213, 885
MboI GATC 2 cut(s) 211, 883
MboII GAAGA 3 cut(s) 90, 638, 938
MhlI GDGCHC 2 cut(s) 63, 957
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 3 cut(s) 293, 683, 908
MluNI TGGCCA 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 552, 723
MnlI CCTC 9 cut(s) 118, 210, 246, 461, 535, 787, 894, 916, 931
Mox20I TGGCCA 1 cut(s) 400
MroI TCCGGA 1 cut(s) 430
MroNI GCCGGC 1 cut(s) 688
MroXI GAANNNNTTC 3 cut(s) 752, 867, 908
MscI TGGCCA 1 cut(s) 400
MseI TTAA 4 cut(s) 74, 342, 420, 561
MslI CAYNNNNRTG 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 400
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 4 cut(s) 32, 431, 689, 830
MspR9I CCNGG 1 cut(s) 831
MwoI GCNNNNNNNGC 3 cut(s) 397, 598, 698
NaeI GCCGGC 1 cut(s) 690
NciI CCSGG 1 cut(s) 831
NdeII GATC 2 cut(s) 211, 883
NgoMIV GCCGGC 1 cut(s) 688
NlaIII CATG 8 cut(s) 29, 301, 512, 592, 679, 741, 760, 784
NlaIV GGNNCC 1 cut(s) 718
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 2 cut(s) 316, 948
PagI TCATGA 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 945
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 690
PdmI GAANNNNTTC 3 cut(s) 752, 867, 908
PfeI GAWTC 3 cut(s) 158, 484, 521
PkrI GCNGC 2 cut(s) 497, 600
Psp124BI GAGCTC 1 cut(s) 957
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 2 cut(s) 585, 717
PstI CTGCAG 1 cut(s) 862
PstNI CAGNNNCTG 3 cut(s) 455, 598, 707
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 1 cut(s) 957
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 4 cut(s) 74, 342, 420, 561
SatI GCNGC 2 cut(s) 496, 599
Sau3AI GATC 2 cut(s) 211, 883
Sau96I GGNCC 2 cut(s) 585, 717
ScrFI CCNGG 1 cut(s) 831
SduI GDGCHC 2 cut(s) 63, 957
SfcI CTRYAG 2 cut(s) 385, 858
SfuI TTCGAA 2 cut(s) 316, 948
SinI GGWCC 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SpeI ACTAGT 1 cut(s) 725
Sse9I AATT 3 cut(s) 293, 683, 908
SsiI CCGC 2 cut(s) 14, 150
SspMI CTAG 2 cut(s) 576, 726
SstI GAGCTC 1 cut(s) 957
StyD4I CCNGG 1 cut(s) 829
TaaI ACNGT 3 cut(s) 8, 322, 643
TaqI TCGA 5 cut(s) 123, 190, 316, 466, 948
TasI AATT 3 cut(s) 293, 683, 908
TfiI GAWTC 3 cut(s) 158, 484, 521
Tru1I TTAA 4 cut(s) 74, 342, 420, 561
Tru9I TTAA 4 cut(s) 74, 342, 420, 561
TscAI CASTG 1 cut(s) 63
TseI GCWGC 2 cut(s) 495, 598
TspDTI ATGAA 7 cut(s) 272, 286, 497, 664, 674, 745, 773
TspGWI ACGGA 2 cut(s) 204, 678
TspRI CASTG 1 cut(s) 63
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 2 cut(s) 293, 908
XceI RCATGY 1 cut(s) 29
XmnI GAANNNNTTC 3 cut(s) 752, 867, 908
XspI CTAG 2 cut(s) 576, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.