Prupe.8G262100_v2.0.a1

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0011175
Physical Location & Seq
Forward (+)
22096749 .. 22099187
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G262100.1

Sequence Viewer

Length: 957 bp
ATGGACTGTTTTGTGGGCTTTGCACATGCCCCGGCTACTGCAATCTTGCGCCGACCGGTAACCTACACTACTTCAAGAACATATAGAAATGGCGGGTTGAAATTGAGAGCAAAGCTCGTCGAAGAAAATGACCCATTACTTCAATCCGCCTTAGATTCAGCCTCTCTTCGTTTCCGAGAAACCCATCGACCAGAGCCTCTCTTTGTTGATCCGTATGCCGGTTGCTTTGTTGCTCCTAATGCTCAGATGGATATGAAGCAATCCTCGGACCATTATTGCCTTGGAACAAAATTCATTGATGATAAGTTGCTCCGTACAGTGAACCATATTGATGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACCCGTCCATATAGGCTTAGTTGGCCAACTTCAACCATTATATTTAATATATCTCCAGAACGGGTATTCAAAAGAGCAGCTGAAAAGCTTCAAGGTGTTGGGGCAAAGATTCCAAGAAGCTGCGTATCCCTTCATGTTCCTTTGGAATCCTCCAACATACAGCAAAGTCTGCGTACTAAAGGCTTTAACGGCAATCGCCCTAGTATATGGGCCATGCAGGGGCTCCCTGTGATGACATTAGCAAGTTTTGAGGACATATTGCTCACTGTAAGTGGTTCTGCCATGAACGGATGTTTTTTCTTGGGAGAATTGCCTGCTTGGTTGGCAGAAACTGAAATAGGGATCAAGACTAGTGCAAGGAAATGGATGGAGAAATTATTCATGAACAATGGCTTTCGGGTGGACATGATTTGCTATGAGGAAGTTGCGCGGAGTTTAGGCAAGGATTTAGCATCAGGAAGCTATAAGAATATACTATTTTCTGCGGAACAACTGCGGTTTTCTGATGATCAGATGGAAACTTGGAGGAAGGAATTTCAAAGAACCGAGGAAGAAGGGGATGAGGAAGGCTTCGAAGAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

319

Amino Acids

35.94

Weight (kDa)

6.04

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 334
AccII CGCG 1 cut(s) 802
AciI CCGC 5 cut(s) 93, 147, 802, 857, 868
AclWI GGATC 2 cut(s) 203, 722
AcoI YGGCCR 1 cut(s) 395
AcsI RAATTY 2 cut(s) 290, 905
AfaI GTAC 2 cut(s) 316, 547
AfiI CCNNNNNNNGG 2 cut(s) 218, 591
AflII CTTAAG 1 cut(s) 338
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 7 cut(s) 75, 100, 143, 405, 442, 464, 911
AhlI ACTAGT 1 cut(s) 722
AluBI AGCT 6 cut(s) 115, 452, 460, 492, 834, 952
AluI AGCT 6 cut(s) 115, 452, 460, 492, 834, 952
Alw21I GWGCWC 1 cut(s) 954
AlwI GGATC 2 cut(s) 203, 722
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 2 cut(s) 395, 582
ApeKI GCWGC 2 cut(s) 449, 492
ApoI RAATTY 2 cut(s) 290, 905
AsiGI ACCGGT 1 cut(s) 55
Asp700I GAANNNNTTC 2 cut(s) 459, 749
AspLEI GCGC 2 cut(s) 51, 802
AspS9I GGNCC 2 cut(s) 268, 582
AsuC2I CCSGG 1 cut(s) 32
AsuII TTCGAA 1 cut(s) 945
AvaII GGWCC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 397
BanII GRGCYC 2 cut(s) 597, 954
Bbv12I GWGCWC 1 cut(s) 954
BbvI GCAGC 2 cut(s) 461, 479
BccI CCATC 6 cut(s) 192, 241, 326, 356, 733, 880
BceAI ACGGC 1 cut(s) 577
BciVI GTATCC 1 cut(s) 508
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 1 cut(s) 32
BcuI ACTAGT 1 cut(s) 722
BfaI CTAG 2 cut(s) 573, 723
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 334
BfuI GTATCC 1 cut(s) 508
BisI GCNGC 2 cut(s) 450, 493
BlsI GCNGC 2 cut(s) 451, 494
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 268, 582
BmiI GGNNCC 1 cut(s) 596
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 833
BoxI GACNNNNGTC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 99, 131
BpmI CTGGAG 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 945
BpuMI CCSGG 1 cut(s) 32
BsaJI CCNNGG 4 cut(s) 30, 264, 280, 918
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 591
Bse118I RCCGGY 2 cut(s) 55, 218
BseDI CCNNGG 4 cut(s) 30, 264, 280, 918
BseGI GGATG 3 cut(s) 668, 744, 937
BseLI CCNNNNNNNGG 2 cut(s) 218, 591
BseMII CTCAG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 461, 479
Bsh1236I CGCG 1 cut(s) 802
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 2 cut(s) 397, 584
BshTI ACCGGT 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 954
BsiSI CCGG 3 cut(s) 32, 56, 219
BslI CCNNNNNNNGG 2 cut(s) 218, 591
BsnI GGCC 2 cut(s) 397, 584
Bsp119I TTCGAA 1 cut(s) 945
Bsp1286I GDGCHC 2 cut(s) 597, 954
Bsp143I GATC 3 cut(s) 208, 714, 880
BspACI CCGC 5 cut(s) 93, 147, 802, 857, 868
BspANI GGCC 2 cut(s) 397, 584
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 802
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 1 cut(s) 596
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 2 cut(s) 203, 722
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 1 cut(s) 945
BspTI CTTAAG 1 cut(s) 338
BsrFI RCCGGY 2 cut(s) 55, 218
BssAI RCCGGY 2 cut(s) 55, 218
BssECI CCNNGG 4 cut(s) 30, 264, 280, 918
BssMI GATC 3 cut(s) 208, 714, 880
BssT1I CCWWGG 1 cut(s) 280
Bst4CI ACNGT 3 cut(s) 8, 319, 640
Bst6I CTCTTC 2 cut(s) 171, 942
BstAFI CTTAAG 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 541
BstBI TTCGAA 1 cut(s) 945
BstC8I GCNNGC 1 cut(s) 687
BstDEI CTNAG 3 cut(s) 151, 243, 389
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 3 cut(s) 668, 744, 937
BstFNI CGCG 1 cut(s) 802
BstHHI GCGC 2 cut(s) 51, 802
BstKTI GATC 3 cut(s) 211, 717, 883
BstMBI GATC 3 cut(s) 208, 714, 880
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 5 cut(s) 239, 394, 541, 561, 695
BstNSI RCATGY 1 cut(s) 29
BstPAI GACNNNNGTC 1 cut(s) 375
BstPI GGTNACC 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 30
BstUI CGCG 1 cut(s) 802
BstV1I GCAGC 2 cut(s) 461, 479
BstXI CCANNNNNNTGG 1 cut(s) 332
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 2 cut(s) 397, 584
BtsCI GGATG 3 cut(s) 668, 744, 937
BtsIMutI CAGTG 2 cut(s) 324, 636
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 687
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 753
CfoI GCGC 2 cut(s) 51, 802
Cfr10I RCCGGY 2 cut(s) 55, 218
Cfr13I GGNCC 2 cut(s) 268, 582
Csp6I GTAC 2 cut(s) 315, 546
CspAI ACCGGT 1 cut(s) 55
CviAII CATG 7 cut(s) 26, 367, 506, 586, 655, 754, 778
CviQI GTAC 2 cut(s) 315, 546
DdeI CTNAG 3 cut(s) 151, 243, 389
DpnI GATC 3 cut(s) 210, 716, 882
DpnII GATC 3 cut(s) 208, 714, 880
EaeI YGGCCR 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 171, 942
EarI CTCTTC 2 cut(s) 171, 942
EciI GGCGGA 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 952
Eco130I CCWWGG 1 cut(s) 280
Eco24I GRGCYC 2 cut(s) 597, 954
Eco47I GGWCC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 952
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 952
EcoO65I GGTNACC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 2 cut(s) 597, 954
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 7 cut(s) 29, 370, 509, 589, 658, 757, 781
FatI CATG 7 cut(s) 25, 366, 505, 585, 654, 753, 777
FauI CCCGC 1 cut(s) 86
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 450, 493
FokI GGATG 3 cut(s) 675, 751, 944
FriOI GRGCYC 2 cut(s) 597, 954
Fsp4HI GCNGC 2 cut(s) 450, 493
FspBI CTAG 2 cut(s) 573, 723
GlaI GCGC 2 cut(s) 50, 801
GluI GCNGC 2 cut(s) 450, 493
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 2 cut(s) 397, 584
HapII CCGG 3 cut(s) 32, 56, 219
HhaI GCGC 2 cut(s) 51, 802
Hin1II CATG 7 cut(s) 29, 370, 509, 589, 658, 757, 781
Hin6I GCGC 2 cut(s) 49, 800
HinP1I GCGC 2 cut(s) 49, 800
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 155, 481, 518
HpaII CCGG 3 cut(s) 32, 56, 219
Hpy166II GTNNAC 2 cut(s) 322, 775
Hpy188I TCNGA 5 cut(s) 176, 246, 268, 877, 885
Hpy188III TCNNGA 5 cut(s) 75, 428, 718, 754, 828
Hpy8I GTNNAC 2 cut(s) 322, 775
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 4 cut(s) 512, 895, 920, 932
HpyCH4III ACNGT 3 cut(s) 8, 319, 640
HpyCH4V TGCA 4 cut(s) 23, 41, 589, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 394, 541, 561, 695
HpyF3I CTNAG 3 cut(s) 151, 243, 389
Hsp92II CATG 7 cut(s) 29, 370, 509, 589, 658, 757, 781
HspAI GCGC 2 cut(s) 49, 800
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 3 cut(s) 208, 714, 880
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 3 cut(s) 238, 315, 600
Lsp1109I GCAGC 2 cut(s) 461, 479
LweI GCATC 1 cut(s) 833
MaeI CTAG 2 cut(s) 573, 723
MaeIII GTNAC 1 cut(s) 58
MalI GATC 3 cut(s) 210, 716, 882
MboI GATC 3 cut(s) 208, 714, 880
MboII GAAGA 3 cut(s) 134, 158, 935
MhlI GDGCHC 2 cut(s) 597, 954
MlsI TGGCCA 1 cut(s) 397
MluCI AATT 5 cut(s) 101, 290, 680, 746, 905
MluNI TGGCCA 1 cut(s) 397
MmeI TCCRAC 1 cut(s) 549
MnlI CCTC 9 cut(s) 172, 207, 274, 532, 616, 784, 891, 913, 928
Mox20I TGGCCA 1 cut(s) 397
MroXI GAANNNNTTC 2 cut(s) 459, 749
MscI TGGCCA 1 cut(s) 397
MseI TTAA 3 cut(s) 339, 417, 558
MslI CAYNNNNRTG 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 397
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 3 cut(s) 32, 56, 219
MspR9I CCNGG 1 cut(s) 32
MvnI CGCG 1 cut(s) 802
MwoI GCNNNNNNNGC 5 cut(s) 239, 394, 541, 561, 695
NciI CCSGG 1 cut(s) 32
NdeII GATC 3 cut(s) 208, 714, 880
NlaIII CATG 7 cut(s) 29, 370, 509, 589, 658, 757, 781
NlaIV GGNNCC 1 cut(s) 596
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 1 cut(s) 945
PagI TCATGA 1 cut(s) 753
PciSI GCTCTTC 1 cut(s) 942
PdmI GAANNNNTTC 2 cut(s) 459, 749
PfeI GAWTC 3 cut(s) 155, 481, 518
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 2 cut(s) 451, 494
PshAI GACNNNNGTC 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 954
PspEI GGTNACC 1 cut(s) 58
PspN4I GGNNCC 1 cut(s) 596
PspPI GGNCC 2 cut(s) 268, 582
PstNI CAGNNNCTG 1 cut(s) 704
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 316, 547
RsaNI GTAC 2 cut(s) 315, 546
RseI CAYNNNNRTG 1 cut(s) 330
SacI GAGCTC 1 cut(s) 954
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 3 cut(s) 339, 417, 558
SatI GCNGC 2 cut(s) 450, 493
Sau3AI GATC 3 cut(s) 208, 714, 880
Sau96I GGNCC 2 cut(s) 268, 582
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 597, 954
SetI ASST 9 cut(s) 65, 117, 348, 454, 462, 469, 494, 836, 954
SfaNI GCATC 1 cut(s) 833
SfuI TTCGAA 1 cut(s) 945
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
SpeI ACTAGT 1 cut(s) 722
Sse9I AATT 5 cut(s) 101, 290, 680, 746, 905
SsiI CCGC 5 cut(s) 93, 147, 802, 857, 868
SspMI CTAG 2 cut(s) 573, 723
SstI GAGCTC 1 cut(s) 954
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 3 cut(s) 8, 319, 640
TaqI TCGA 3 cut(s) 120, 187, 945
TasI AATT 5 cut(s) 101, 290, 680, 746, 905
TfiI GAWTC 3 cut(s) 155, 481, 518
Tru1I TTAA 3 cut(s) 339, 417, 558
Tru9I TTAA 3 cut(s) 339, 417, 558
TscAI CASTG 2 cut(s) 324, 643
TseI GCWGC 2 cut(s) 449, 492
TspDTI ATGAA 6 cut(s) 269, 283, 494, 671, 742, 770
TspGWI ACGGA 3 cut(s) 201, 302, 675
TspRI CASTG 2 cut(s) 324, 643
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 2 cut(s) 290, 905
XceI RCATGY 1 cut(s) 29
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XmnI GAANNNNTTC 2 cut(s) 459, 749
XspI CTAG 2 cut(s) 573, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.