Rh6CG368000

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011175
Physical Location & Seq
Forward (+)
55568595 .. 55571431
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG368000.1

Sequence Viewer

Length: 882 bp
ATGGACTCTTTACTGGGCTTCCCACAATCCCCAGCTACTGCCATCTTGCGCCGCCCTCTAATCTCCACTTCAAGACCAAACAGTAACAGAAATGCCGGGTTGAGTTTCAGAGCAAAACCCAACGACGCAATTGACCCATTACTCCACGACGCCGTTAATTCAGCTTCTCTTCGTTTCCGAGAGACCCATCGACCAGGCAACCCCTTAACCTCCGTATTTATTGCTGTATTTTTCTGTCAGTCATTTCGTGATAAGCAATTCAGTACAATACACAAATGTGACATTTTTGTTTGGCATTGTGGGGTTTGTGAGTATTTGGTTAATACTTTATGTGCAGAGCCTCTCTTTGTTGATCCATATGCCGGTTGCTTTGTTGATCCTAATGCGCAGATGGACATGAAGCAATGCTCACACCATTATTGCATTGCAACCAAGTACATTGATGATAAGTTGCTTCGTACAGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACCCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATAATTTTTGATATTTCGCCGGACCGGGTATTCAGTGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATACCAAGAAGCTGCTTATTCCTTCATGTTCCATTGGAGTCCTCTGATTTGCAGCAAACTCTACGTGCTAAAGGCTTCAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCCGTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTAAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGGTTGGTTGGCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

293

Amino Acids

32.61

Weight (kDa)

7.57

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 126 - 247 4.9e-22 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 478
AciI CCGC 1 cut(s) 52
AclWI GGATC 2 cut(s) 347, 371
AcoI YGGCCR 2 cut(s) 521, 539
AcsI RAATTY 1 cut(s) 760
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 3 cut(s) 265, 437, 460
AfiI CCNNNNNNNGG 2 cut(s) 362, 475
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 3 cut(s) 72, 549, 703
AjnI CCWGG 1 cut(s) 193
AleI CACNNNNGTG 1 cut(s) 276
AluBI AGCT 5 cut(s) 35, 164, 596, 604, 636
AluI AGCT 5 cut(s) 35, 164, 596, 604, 636
Alw26I GTCTC 2 cut(s) 176, 839
AlwI GGATC 2 cut(s) 347, 371
AlwNI CAGNNNCTG 3 cut(s) 38, 739, 848
AoxI GGCC 4 cut(s) 521, 539, 713, 726
ApeKI GCWGC 3 cut(s) 593, 636, 676
ApoI RAATTY 1 cut(s) 760
AspLEI GCGC 2 cut(s) 51, 388
AspS9I GGNCC 2 cut(s) 574, 726
AsuC2I CCSGG 3 cut(s) 97, 520, 578
AvaII GGWCC 1 cut(s) 574
BalI TGGCCA 1 cut(s) 541
BbvI GCAGC 3 cut(s) 605, 623, 688
BccI CCATC 4 cut(s) 50, 195, 385, 500
BceAI ACGGC 2 cut(s) 137, 508
BciT130I CCWGG 1 cut(s) 195
BcnI CCSGG 3 cut(s) 97, 520, 578
BcoDI GTCTC 2 cut(s) 176, 839
BfaI CTAG 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 478
BglI GCCNNNNNGGC 1 cut(s) 529
BisI GCNGC 4 cut(s) 52, 594, 637, 677
BlsI GCNGC 4 cut(s) 53, 595, 638, 678
Bme1390I CCNGG 4 cut(s) 97, 195, 520, 578
Bme18I GGWCC 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 574, 726
BmrFI CCNGG 4 cut(s) 97, 195, 520, 578
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BpuMI CCSGG 3 cut(s) 97, 520, 578
BsaAI YACGTR 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 861
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 475
Bse118I RCCGGY 2 cut(s) 362, 711
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 2 cut(s) 410, 423
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 861
BseGI GGATG 1 cut(s) 812
BseLI CCNNNNNNNGG 2 cut(s) 362, 475
BseMI GCAATG 2 cut(s) 410, 423
BseNI ACTGG 1 cut(s) 18
BseX3I CGGCCG 1 cut(s) 521
BseXI GCAGC 3 cut(s) 605, 623, 688
BseYI CCCAGC 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 354
Bsh1285I CGRYCG 1 cut(s) 524
BshFI GGCC 4 cut(s) 523, 541, 715, 728
BsiEI CGRYCG 1 cut(s) 524
BsiSI CCGG 6 cut(s) 96, 363, 520, 572, 577, 712
BslFI GGGAC 2 cut(s) 492, 750
BslI CCNNNNNNNGG 2 cut(s) 362, 475
BsmAI GTCTC 2 cut(s) 176, 839
BsmFI GGGAC 2 cut(s) 492, 750
BsnI GGCC 4 cut(s) 523, 541, 715, 728
Bso31I GGTCTC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 352, 376
BspACI CCGC 1 cut(s) 52
BspANI GGCC 4 cut(s) 523, 541, 715, 728
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 2 cut(s) 347, 371
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 410, 423
BsrFI RCCGGY 2 cut(s) 362, 711
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 2 cut(s) 362, 711
BssECI CCNNGG 1 cut(s) 861
BssMI GATC 2 cut(s) 352, 376
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 3 cut(s) 83, 463, 784
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 739
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 1 cut(s) 533
BstEII GGTNACC 1 cut(s) 707
BstF5I GGATG 1 cut(s) 812
BstHHI GCGC 2 cut(s) 51, 388
BstKTI GATC 2 cut(s) 355, 379
BstMAI GTCTC 2 cut(s) 176, 839
BstMBI GATC 2 cut(s) 352, 376
BstMCI CGRYCG 1 cut(s) 524
BstMWI GCNNNNNNNGC 4 cut(s) 529, 538, 739, 839
BstNI CCWGG 1 cut(s) 195
BstPI GGTNACC 1 cut(s) 707
BstSCI CCNGG 4 cut(s) 95, 193, 518, 576
BstV1I GCAGC 3 cut(s) 605, 623, 688
BstZI CGGCCG 1 cut(s) 521
BsuRI GGCC 4 cut(s) 523, 541, 715, 728
BtsCI GGATG 1 cut(s) 812
BtsIMutI CAGTG 3 cut(s) 468, 592, 780
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 1 cut(s) 831
CaiI CAGNNNCTG 3 cut(s) 38, 739, 848
CfoI GCGC 2 cut(s) 51, 388
Cfr10I RCCGGY 2 cut(s) 362, 711
Cfr13I GGNCC 2 cut(s) 574, 726
CpoI CGGWCCG 1 cut(s) 574
CseI GACGC 2 cut(s) 134, 158
Csp6I GTAC 3 cut(s) 264, 436, 459
CspI CGGWCCG 1 cut(s) 574
CviAII CATG 5 cut(s) 397, 511, 650, 730, 799
CviQI GTAC 3 cut(s) 264, 436, 459
DdeI CTNAG 1 cut(s) 533
DpnI GATC 2 cut(s) 354, 378
DpnII GATC 2 cut(s) 352, 376
EaeI YGGCCR 2 cut(s) 521, 539
EagI CGGCCG 1 cut(s) 521
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 574
Eco52I CGGCCG 1 cut(s) 521
Eco91I GGTNACC 1 cut(s) 707
EcoO65I GGTNACC 1 cut(s) 707
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 5 cut(s) 400, 514, 653, 733, 802
FaqI GGGAC 2 cut(s) 492, 750
FatI CATG 5 cut(s) 396, 510, 649, 729, 798
FauNDI CATATG 1 cut(s) 358
Fnu4HI GCNGC 4 cut(s) 52, 594, 637, 677
FokI GGATG 1 cut(s) 819
Fsp4HI GCNGC 4 cut(s) 52, 594, 637, 677
FspBI CTAG 1 cut(s) 717
FspI TGCGCA 1 cut(s) 387
GlaI GCGC 2 cut(s) 50, 387
GluI GCNGC 4 cut(s) 52, 594, 637, 677
GsaI CCCAGC 1 cut(s) 35
HaeIII GGCC 4 cut(s) 523, 541, 715, 728
HapII CCGG 6 cut(s) 96, 363, 520, 572, 577, 712
HgaI GACGC 2 cut(s) 134, 158
HhaI GCGC 2 cut(s) 51, 388
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 5 cut(s) 400, 514, 653, 733, 802
Hin6I GCGC 2 cut(s) 49, 386
HinP1I GCGC 2 cut(s) 49, 386
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 2 cut(s) 5, 662
HpaII CCGG 6 cut(s) 96, 363, 520, 572, 577, 712
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 3 cut(s) 110, 179, 670
Hpy188III TCNNGA 2 cut(s) 72, 248
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 2 cut(s) 128, 152
HpyAV CCTTC 1 cut(s) 656
HpyCH4III ACNGT 3 cut(s) 83, 463, 784
HpyCH4IV ACGT 1 cut(s) 688
HpyCH4V TGCA 5 cut(s) 335, 423, 428, 676, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 529, 538, 739, 839
HpyF3I CTNAG 1 cut(s) 533
HpySE526I ACGT 1 cut(s) 688
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 5 cut(s) 400, 514, 653, 733, 802
HspAI GCGC 2 cut(s) 49, 386
Kzo9I GATC 2 cut(s) 352, 376
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 3 cut(s) 605, 623, 688
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 688
MaeIII GTNAC 4 cut(s) 83, 278, 707, 856
MalI GATC 2 cut(s) 354, 378
MboI GATC 2 cut(s) 352, 376
MboII GAAGA 1 cut(s) 161
MfeI CAATTG 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 541
MluCI AATT 6 cut(s) 129, 157, 257, 555, 760, 824
MluNI TGGCCA 1 cut(s) 541
MlyI GAGTC 1 cut(s) 671
MnlI CCTC 6 cut(s) 66, 220, 351, 469, 676, 760
Mox20I TGGCCA 1 cut(s) 541
MscI TGGCCA 1 cut(s) 541
MseI TTAA 6 cut(s) 156, 206, 321, 483, 606, 880
MslI CAYNNNNRTG 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 541
MspA1I CMGCKG 1 cut(s) 596
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 6 cut(s) 96, 363, 520, 572, 577, 712
MspR9I CCNGG 4 cut(s) 97, 195, 520, 578
MunI CAATTG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 4 cut(s) 529, 538, 739, 839
NciI CCSGG 3 cut(s) 97, 520, 578
NdeI CATATG 1 cut(s) 358
NdeII GATC 2 cut(s) 352, 376
NlaIII CATG 5 cut(s) 400, 514, 653, 733, 802
NmuCI GTSAC 2 cut(s) 278, 856
NsbI TGCGCA 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 276
PkrI GCNGC 4 cut(s) 53, 595, 638, 678
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 689
Psp6I CCWGG 1 cut(s) 193
PspEI GGTNACC 1 cut(s) 707
PspFI CCCAGC 1 cut(s) 31
PspGI CCWGG 1 cut(s) 193
PspPI GGNCC 2 cut(s) 574, 726
PstNI CAGNNNCTG 3 cut(s) 38, 739, 848
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 3 cut(s) 265, 437, 460
RsaNI GTAC 3 cut(s) 264, 436, 459
RseI CAYNNNNRTG 1 cut(s) 276
Rsr2I CGGWCCG 1 cut(s) 574
RsrII CGGWCCG 1 cut(s) 574
SaqAI TTAA 6 cut(s) 156, 206, 321, 483, 606, 880
SatI GCNGC 4 cut(s) 52, 594, 637, 677
Sau3AI GATC 2 cut(s) 352, 376
Sau96I GGNCC 2 cut(s) 574, 726
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 4 cut(s) 97, 195, 520, 578
SinI GGWCC 1 cut(s) 574
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 6 cut(s) 129, 157, 257, 555, 760, 824
SsiI CCGC 1 cut(s) 52
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 95, 193, 518, 576
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 3 cut(s) 83, 463, 784
TaiI ACGT 1 cut(s) 691
TaqI TCGA 1 cut(s) 190
TasI AATT 6 cut(s) 129, 157, 257, 555, 760, 824
TatI WGTACW 2 cut(s) 263, 435
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 6 cut(s) 156, 206, 321, 483, 606, 880
Tru9I TTAA 6 cut(s) 156, 206, 321, 483, 606, 880
TscAI CASTG 3 cut(s) 468, 592, 787
TseFI GTSAC 2 cut(s) 278, 856
TseI GCWGC 3 cut(s) 593, 636, 676
Tsp45I GTSAC 2 cut(s) 278, 856
TspDTI ATGAA 3 cut(s) 413, 638, 815
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 3 cut(s) 468, 592, 787
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 1 cut(s) 574
XapI RAATTY 1 cut(s) 760
XspI CTAG 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.