Rw6G030840

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011175
Physical Location & Seq
Forward (+)
54905263 .. 54907744
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G030840.1

Sequence Viewer

Length: 960 bp
ATGGACTCTTTACTGGGCTTCCCACAATCCCCAGCTACTGCCATCTTGCGCCGCCCGCTAATCTCCACTTCAAGACCAAACAGTAACAGAAATGCCGGGTTGAGTTTCAGAGCAAAACCCAACGACGCAATTGACCCATTACTCCACGACGCCGTTAATTCAGCTTCTCTTCGTTTCCGAGAGACCCATCGACCAGAGCCTCTCTTTGTTGATCCATATGCCGGTTGCTTTGTTGATCCTAATGCGCAGATGGACATGAAGCAATGCTCACACCATTATTGCATTGCAACTAAGTACATTGATGATAAGTTACTTCGTACAGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACCCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATAATTTTTGATATTTCGCCGGACCGGGTATTCAGTGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGCTGCTTATTCCTTCATGTTCCATTGGAGTCCTCTGATTTGCAGCAAACTCTACGTGCTAAAGGCTTCAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCCGTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTTAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGACTAGTACAAGGGAATGGATGGAGAAATTATTCATGAACAACGGCTTTCGGGTGGACATGATTCGCTATGAAGAAGTTGCAAAGAGTTTAGGCAAGAAATTAGCAGCAGGACCTTATAAGAATATACTATTTTCCTCAGAACAACTGCGGTTTTCTGATGATCAGATGGAAACTTGGAGAAGGGAATTTCAAAGAGTTGAGGAACAAGGGGATGAAGAAGGTTTTGAAGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

319

Amino Acids

36.04

Weight (kDa)

5.94

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 51 - 200 8.7e-24 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 337
AciI CCGC 3 cut(s) 52, 56, 871
AclWI GGATC 2 cut(s) 206, 230
AcoI YGGCCR 2 cut(s) 380, 398
AcsI RAATTY 2 cut(s) 619, 908
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 3 cut(s) 296, 319, 730
AfiI CCNNNNNNNGG 2 cut(s) 221, 334
AflII CTTAAG 1 cut(s) 341
AgsI TTSAA 5 cut(s) 72, 408, 562, 914, 950
AhlI ACTAGT 1 cut(s) 725
AluBI AGCT 6 cut(s) 35, 164, 455, 463, 495, 955
AluI AGCT 6 cut(s) 35, 164, 455, 463, 495, 955
Alw26I GTCTC 2 cut(s) 176, 698
AlwI GGATC 2 cut(s) 206, 230
AlwNI CAGNNNCTG 3 cut(s) 38, 598, 707
AoxI GGCC 4 cut(s) 380, 398, 572, 585
ApeKI GCWGC 4 cut(s) 452, 495, 535, 827
ApoI RAATTY 2 cut(s) 619, 908
Asp700I GAANNNNTTC 1 cut(s) 752
AspLEI GCGC 2 cut(s) 51, 247
AspS9I GGNCC 3 cut(s) 433, 585, 833
AsuC2I CCSGG 3 cut(s) 97, 379, 437
AvaII GGWCC 2 cut(s) 433, 833
BalI TGGCCA 1 cut(s) 400
BbvI GCAGC 4 cut(s) 464, 482, 547, 839
BccI CCATC 6 cut(s) 50, 195, 244, 359, 736, 883
BceAI ACGGC 3 cut(s) 137, 367, 781
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 3 cut(s) 97, 379, 437
BcoDI GTCTC 2 cut(s) 176, 698
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 2 cut(s) 576, 726
BfrI CTTAAG 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 388
BisI GCNGC 5 cut(s) 52, 453, 496, 536, 828
BlsI GCNGC 5 cut(s) 53, 454, 497, 537, 829
Bme1390I CCNGG 3 cut(s) 97, 379, 437
Bme18I GGWCC 2 cut(s) 433, 833
BmgT120I GGNCC 3 cut(s) 433, 585, 833
BmrFI CCNGG 3 cut(s) 97, 379, 437
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BpuMI CCSGG 3 cut(s) 97, 379, 437
BsaAI YACGTR 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 176
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 334
Bse118I RCCGGY 2 cut(s) 221, 570
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 2 cut(s) 269, 282
BseGI GGATG 3 cut(s) 671, 747, 940
BseLI CCNNNNNNNGG 2 cut(s) 221, 334
BseMI GCAATG 2 cut(s) 269, 282
BseMII CTCAG 1 cut(s) 873
BseNI ACTGG 1 cut(s) 18
BseX3I CGGCCG 1 cut(s) 380
BseXI GCAGC 4 cut(s) 464, 482, 547, 839
BseYI CCCAGC 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 4 cut(s) 382, 400, 574, 587
BsiEI CGRYCG 1 cut(s) 383
BsiSI CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
BslFI GGGAC 2 cut(s) 351, 609
BslI CCNNNNNNNGG 2 cut(s) 221, 334
BsmAI GTCTC 2 cut(s) 176, 698
BsmFI GGGAC 2 cut(s) 351, 609
BsnI GGCC 4 cut(s) 382, 400, 574, 587
Bso31I GGTCTC 1 cut(s) 176
Bsp143I GATC 3 cut(s) 211, 235, 883
BspACI CCGC 3 cut(s) 52, 56, 871
BspANI GGCC 4 cut(s) 382, 400, 574, 587
BspCNI CTCAG 1 cut(s) 872
BspHI TCATGA 1 cut(s) 756
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 206, 230
BspQI GCTCTTC 1 cut(s) 945
BspTI CTTAAG 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 269, 282
BsrFI RCCGGY 2 cut(s) 221, 570
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 2 cut(s) 221, 570
BssMI GATC 3 cut(s) 211, 235, 883
BssNI GRCGYC 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 83, 322, 643
Bst6I CTCTTC 2 cut(s) 174, 945
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 598
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 56, 690
BstDEI CTNAG 3 cut(s) 291, 392, 859
BstEII GGTNACC 1 cut(s) 566
BstF5I GGATG 3 cut(s) 671, 747, 940
BstHHI GCGC 2 cut(s) 51, 247
BstKTI GATC 3 cut(s) 214, 238, 886
BstMAI GTCTC 2 cut(s) 176, 698
BstMBI GATC 3 cut(s) 211, 235, 883
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 5 cut(s) 55, 388, 397, 598, 698
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 3 cut(s) 95, 377, 435
BstV1I GCAGC 4 cut(s) 464, 482, 547, 839
BstZI CGGCCG 1 cut(s) 380
BsuRI GGCC 4 cut(s) 382, 400, 574, 587
BtsCI GGATG 3 cut(s) 671, 747, 940
BtsIMutI CAGTG 3 cut(s) 327, 451, 639
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 2 cut(s) 56, 690
CaiI CAGNNNCTG 3 cut(s) 38, 598, 707
CciI TCATGA 1 cut(s) 756
CfoI GCGC 2 cut(s) 51, 247
Cfr10I RCCGGY 2 cut(s) 221, 570
Cfr13I GGNCC 3 cut(s) 433, 585, 833
CpoI CGGWCCG 1 cut(s) 433
CseI GACGC 2 cut(s) 134, 158
Csp6I GTAC 3 cut(s) 295, 318, 729
CspI CGGWCCG 1 cut(s) 433
CviAII CATG 7 cut(s) 256, 370, 509, 589, 658, 757, 781
CviQI GTAC 3 cut(s) 295, 318, 729
DdeI CTNAG 3 cut(s) 291, 392, 859
DpnI GATC 3 cut(s) 213, 237, 885
DpnII GATC 3 cut(s) 211, 235, 883
EaeI YGGCCR 2 cut(s) 380, 398
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 2 cut(s) 174, 945
EarI CTCTTC 2 cut(s) 174, 945
EclXI CGGCCG 1 cut(s) 380
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 433, 833
Eco52I CGGCCG 1 cut(s) 380
Eco91I GGTNACC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 833
EcoO65I GGTNACC 1 cut(s) 566
FaeI CATG 7 cut(s) 259, 373, 512, 592, 661, 760, 784
FaqI GGGAC 2 cut(s) 351, 609
FatI CATG 7 cut(s) 255, 369, 508, 588, 657, 756, 780
FauI CCCGC 1 cut(s) 63
FauNDI CATATG 1 cut(s) 217
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 5 cut(s) 52, 453, 496, 536, 828
FokI GGATG 3 cut(s) 678, 754, 947
Fsp4HI GCNGC 5 cut(s) 52, 453, 496, 536, 828
FspBI CTAG 2 cut(s) 576, 726
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 2 cut(s) 50, 246
GluI GCNGC 5 cut(s) 52, 453, 496, 536, 828
GsaI CCCAGC 1 cut(s) 35
HaeIII GGCC 4 cut(s) 382, 400, 574, 587
HapII CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
HgaI GACGC 2 cut(s) 134, 158
HhaI GCGC 2 cut(s) 51, 247
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 7 cut(s) 259, 373, 512, 592, 661, 760, 784
Hin6I GCGC 2 cut(s) 49, 245
HinP1I GCGC 2 cut(s) 49, 245
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 3 cut(s) 5, 521, 784
HpaII CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
Hpy166II GTNNAC 2 cut(s) 325, 778
Hpy188I TCNGA 6 cut(s) 110, 179, 529, 862, 880, 888
Hpy188III TCNNGA 2 cut(s) 72, 757
Hpy8I GTNNAC 2 cut(s) 325, 778
Hpy99I CGWCG 2 cut(s) 128, 152
HpyAV CCTTC 3 cut(s) 515, 897, 935
HpyCH4III ACNGT 3 cut(s) 83, 322, 643
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 5 cut(s) 282, 287, 535, 592, 803
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 388, 397, 598, 698
HpyF3I CTNAG 3 cut(s) 291, 392, 859
HpySE526I ACGT 1 cut(s) 547
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 7 cut(s) 259, 373, 512, 592, 661, 760, 784
HspAI GCGC 2 cut(s) 49, 245
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 3 cut(s) 211, 235, 883
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 4 cut(s) 464, 482, 547, 839
MaeI CTAG 2 cut(s) 576, 726
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 4 cut(s) 83, 309, 566, 715
MalI GATC 3 cut(s) 213, 237, 885
MboI GATC 3 cut(s) 211, 235, 883
MboII GAAGA 3 cut(s) 161, 806, 950
MfeI CAATTG 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 8 cut(s) 129, 157, 414, 619, 683, 749, 821, 908
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 1 cut(s) 530
MnlI CCTC 6 cut(s) 210, 328, 535, 619, 868, 916
Mox20I TGGCCA 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 752
MscI TGGCCA 1 cut(s) 400
MseI TTAA 3 cut(s) 156, 342, 465
Msp20I TGGCCA 1 cut(s) 400
MspA1I CMGCKG 1 cut(s) 455
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
MspR9I CCNGG 3 cut(s) 97, 379, 437
MunI CAATTG 1 cut(s) 129
MwoI GCNNNNNNNGC 5 cut(s) 55, 388, 397, 598, 698
NciI CCSGG 3 cut(s) 97, 379, 437
NdeI CATATG 1 cut(s) 217
NdeII GATC 3 cut(s) 211, 235, 883
NlaIII CATG 7 cut(s) 259, 373, 512, 592, 661, 760, 784
NmuCI GTSAC 1 cut(s) 715
NsbI TGCGCA 1 cut(s) 246
PagI TCATGA 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 945
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 1 cut(s) 784
PkrI GCNGC 5 cut(s) 53, 454, 497, 537, 829
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 833
PsiI TTATAA 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 833
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 1 cut(s) 31
PspPI GGNCC 3 cut(s) 433, 585, 833
PspPPI RGGWCCY 1 cut(s) 833
PstNI CAGNNNCTG 3 cut(s) 38, 598, 707
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 3 cut(s) 296, 319, 730
RsaNI GTAC 3 cut(s) 295, 318, 729
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 3 cut(s) 156, 342, 465
SatI GCNGC 5 cut(s) 52, 453, 496, 536, 828
Sau3AI GATC 3 cut(s) 211, 235, 883
Sau96I GGNCC 3 cut(s) 433, 585, 833
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 3 cut(s) 97, 379, 437
SinI GGWCC 2 cut(s) 433, 833
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SpeI ACTAGT 1 cut(s) 725
Sse9I AATT 8 cut(s) 129, 157, 414, 619, 683, 749, 821, 908
SsiI CCGC 3 cut(s) 52, 56, 871
SspMI CTAG 2 cut(s) 576, 726
StyD4I CCNGG 3 cut(s) 95, 377, 435
TaaI ACNGT 3 cut(s) 83, 322, 643
TaiI ACGT 1 cut(s) 550
TaqI TCGA 1 cut(s) 190
TasI AATT 8 cut(s) 129, 157, 414, 619, 683, 749, 821, 908
TatI WGTACW 2 cut(s) 294, 728
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 1 cut(s) 784
Tru1I TTAA 3 cut(s) 156, 342, 465
Tru9I TTAA 3 cut(s) 156, 342, 465
TscAI CASTG 3 cut(s) 327, 451, 646
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 4 cut(s) 452, 495, 535, 827
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 7 cut(s) 272, 497, 674, 745, 773, 807, 951
TspRI CASTG 3 cut(s) 327, 451, 646
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 2 cut(s) 433, 833
XapI RAATTY 2 cut(s) 619, 908
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 2 cut(s) 576, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.