Rh6DG354700

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011175
Physical Location & Seq
Forward (+)
55355416 .. 55357993
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG354700.1

Sequence Viewer

Length: 1038 bp
ATGATTAAGGTCCTCGCGCTCCACGGAGGGAAGCTGTCTCCCACTCTAACCCCTCTATACGGAGCTGAATGGACGGACGATGAATACTCCTATTCCCACACGTACGGCTTATCATCATCGGAATTGTATATGGACTCTTTACTGGGCTTCCCACAATCCCCAGCTACCGCCATCTTGCGCCGCCCGCTAATCTCCACTTCAAGACCAAACAGTAACAGAAATGCCGGGTTGAGTTTCAGAGCAAAACCCAACGACGCAATTGACCCATTACTCCACGACGCCGTTAATTCAGCTTCTCTTCGTTTCCGAGAGACCCATCGACCAGAGCCTCTCTTTGTTGATCCGTATGCCGGTTGCTTTGTTGATCCTAATGCGCAGATGGACATGAAGCAATGCTCACACCATTATTGCATTGCAACTAAGTACATTGATGATAAGTTGCTTCGTACGGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACTCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATTATTTTTGATATTTCGCCGGACCGGGTATTCAATGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGCTGCTTATTCCTTCATGTTCCATTGGAGTCCTCCGATTTGCAGCAAACTCTACGTGCTAAAGGCTTCAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCCGTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTAAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGACTAGTACAAGGGAATGGATGGAGAAATTATTCATGAACAATGGCTTTCGGGTGGACATGATTCACTATGAAGAAGTTGCAAAGAGCTTAGGCAAGAAATTAGCAGCAGGACCTTATAAGAATATACTATTTTCCTCAGAACAACTGCGGTTTTCTGATGATCAGGTATGGTGTTCTTCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

345

Amino Acids

38.57

Weight (kDa)

6.51

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 94 - 243 1e-23 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 969
Acc16I TGCGCA 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 466
AccII CGCG 1 cut(s) 17
AciI CCGC 4 cut(s) 168, 181, 185, 1000
AclWI GGATC 2 cut(s) 335, 359
AcoI YGGCCR 2 cut(s) 509, 527
AcsI RAATTY 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 1014
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 104, 425, 448, 859
AfiI CCNNNNNNNGG 3 cut(s) 59, 350, 463
AflII CTTAAG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 4 cut(s) 201, 537, 574, 691
AhlI ACTAGT 1 cut(s) 854
AluBI AGCT 8 cut(s) 34, 65, 164, 293, 584, 592, 624, 939
AluI AGCT 8 cut(s) 34, 65, 164, 293, 584, 592, 624, 939
Alw26I GTCTC 3 cut(s) 42, 305, 827
AlwI GGATC 2 cut(s) 335, 359
AlwNI CAGNNNCTG 2 cut(s) 727, 836
AoxI GGCC 4 cut(s) 509, 527, 701, 714
ApeKI GCWGC 4 cut(s) 581, 624, 664, 956
ApoI RAATTY 1 cut(s) 748
Asp700I GAANNNNTTC 1 cut(s) 881
AspLEI GCGC 3 cut(s) 19, 180, 376
AspS9I GGNCC 4 cut(s) 10, 562, 714, 962
AsuC2I CCSGG 2 cut(s) 226, 566
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 3 cut(s) 10, 562, 962
BalI TGGCCA 1 cut(s) 529
BbvI GCAGC 4 cut(s) 593, 611, 676, 968
BccI CCATC 5 cut(s) 179, 324, 373, 488, 865
BceAI ACGGC 3 cut(s) 121, 266, 496
BclI TGATCA 1 cut(s) 1012
BcnI CCSGG 2 cut(s) 226, 566
BcoDI GTCTC 3 cut(s) 42, 305, 827
BcuI ACTAGT 1 cut(s) 854
BfaI CTAG 2 cut(s) 705, 855
BfrI CTTAAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 466
BglI GCCNNNNNGGC 1 cut(s) 517
BisI GCNGC 5 cut(s) 181, 582, 625, 665, 957
BlsI GCNGC 5 cut(s) 182, 583, 626, 666, 958
Bme1390I CCNGG 2 cut(s) 226, 566
Bme18I GGWCC 3 cut(s) 10, 562, 962
BmgT120I GGNCC 4 cut(s) 10, 562, 714, 962
BmrFI CCNGG 2 cut(s) 226, 566
BmrI ACTGGG 1 cut(s) 152
BmuI ACTGGG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 940
BpuMI CCSGG 2 cut(s) 226, 566
BsaAI YACGTR 2 cut(s) 102, 677
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 22
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 350, 463
Bse118I RCCGGY 2 cut(s) 350, 699
Bse1I ACTGG 1 cut(s) 147
Bse3DI GCAATG 2 cut(s) 398, 411
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 2 cut(s) 800, 876
BseLI CCNNNNNNNGG 3 cut(s) 59, 350, 463
BseMI GCAATG 2 cut(s) 398, 411
BseMII CTCAG 1 cut(s) 1002
BseNI ACTGG 1 cut(s) 147
BseX3I CGGCCG 1 cut(s) 509
BseXI GCAGC 4 cut(s) 593, 611, 676, 968
BseYI CCCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 512
BshFI GGCC 4 cut(s) 511, 529, 703, 716
BsiEI CGRYCG 1 cut(s) 512
BsiSI CCGG 5 cut(s) 225, 351, 560, 565, 700
BsiWI CGTACG 2 cut(s) 102, 446
BslFI GGGAC 2 cut(s) 480, 738
BslI CCNNNNNNNGG 3 cut(s) 59, 350, 463
BsmAI GTCTC 3 cut(s) 42, 305, 827
BsmFI GGGAC 2 cut(s) 480, 738
BsnI GGCC 4 cut(s) 511, 529, 703, 716
Bso31I GGTCTC 1 cut(s) 305
Bsp143I GATC 3 cut(s) 340, 364, 1012
BspACI CCGC 4 cut(s) 168, 181, 185, 1000
BspANI GGCC 4 cut(s) 511, 529, 703, 716
BspCNI CTCAG 1 cut(s) 1001
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 885
BspMI ACCTGC 1 cut(s) 466
BspPI GGATC 2 cut(s) 335, 359
BspTI CTTAAG 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 398, 411
BsrFI RCCGGY 2 cut(s) 350, 699
BsrI ACTGG 1 cut(s) 147
BssAI RCCGGY 2 cut(s) 350, 699
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 3 cut(s) 340, 364, 1012
BssNI GRCGYC 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 212, 451, 772
Bst6I CTCTTC 1 cut(s) 303
BstACI GRCGYC 1 cut(s) 279
BstAFI CTTAAG 1 cut(s) 470
BstAPI GCANNNNNTGC 1 cut(s) 727
BstBAI YACGTR 2 cut(s) 102, 677
BstC8I GCNNGC 2 cut(s) 185, 819
BstDEI CTNAG 4 cut(s) 420, 521, 940, 988
BstDSI CCRYGG 1 cut(s) 22
BstEII GGTNACC 1 cut(s) 695
BstF5I GGATG 2 cut(s) 800, 876
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 3 cut(s) 19, 180, 376
BstKTI GATC 3 cut(s) 343, 367, 1015
BstMAI GTCTC 3 cut(s) 42, 305, 827
BstMBI GATC 3 cut(s) 340, 364, 1012
BstMCI CGRYCG 1 cut(s) 512
BstMWI GCNNNNNNNGC 5 cut(s) 184, 517, 526, 727, 827
BstPI GGTNACC 1 cut(s) 695
BstSCI CCNGG 2 cut(s) 224, 564
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 593, 611, 676, 968
BstZI CGGCCG 1 cut(s) 509
BsuRI GGCC 4 cut(s) 511, 529, 703, 716
BtgI CCRYGG 1 cut(s) 22
BtsCI GGATG 2 cut(s) 800, 876
BtsIMutI CAGTG 1 cut(s) 768
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 2 cut(s) 185, 819
CaiI CAGNNNCTG 2 cut(s) 727, 836
CciI TCATGA 1 cut(s) 885
CfoI GCGC 3 cut(s) 19, 180, 376
Cfr10I RCCGGY 2 cut(s) 350, 699
Cfr13I GGNCC 4 cut(s) 10, 562, 714, 962
CpoI CGGWCCG 1 cut(s) 562
CseI GACGC 2 cut(s) 263, 287
Csp6I GTAC 4 cut(s) 103, 424, 447, 858
CspI CGGWCCG 1 cut(s) 562
CviAII CATG 7 cut(s) 385, 499, 638, 718, 787, 886, 910
CviQI GTAC 4 cut(s) 103, 424, 447, 858
DdeI CTNAG 4 cut(s) 420, 521, 940, 988
DpnI GATC 3 cut(s) 342, 366, 1014
DpnII GATC 3 cut(s) 340, 364, 1012
EaeI YGGCCR 2 cut(s) 509, 527
EagI CGGCCG 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
EclXI CGGCCG 1 cut(s) 509
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 3 cut(s) 10, 562, 962
Eco52I CGGCCG 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 1014
Eco91I GGTNACC 1 cut(s) 695
EcoO109I RGGNCCY 2 cut(s) 10, 962
EcoO65I GGTNACC 1 cut(s) 695
FaeI CATG 7 cut(s) 388, 502, 641, 721, 790, 889, 913
FaqI GGGAC 2 cut(s) 480, 738
FatI CATG 7 cut(s) 384, 498, 637, 717, 786, 885, 909
FauI CCCGC 1 cut(s) 192
FbaI TGATCA 1 cut(s) 1012
Fnu4HI GCNGC 5 cut(s) 181, 582, 625, 665, 957
FokI GGATG 2 cut(s) 807, 883
Fsp4HI GCNGC 5 cut(s) 181, 582, 625, 665, 957
FspBI CTAG 2 cut(s) 705, 855
FspI TGCGCA 1 cut(s) 375
GlaI GCGC 3 cut(s) 18, 179, 375
GluI GCNGC 5 cut(s) 181, 582, 625, 665, 957
GsaI CCCAGC 1 cut(s) 164
HaeIII GGCC 4 cut(s) 511, 529, 703, 716
HapII CCGG 5 cut(s) 225, 351, 560, 565, 700
HgaI GACGC 2 cut(s) 263, 287
HhaI GCGC 3 cut(s) 19, 180, 376
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 7 cut(s) 388, 502, 641, 721, 790, 889, 913
Hin6I GCGC 3 cut(s) 17, 178, 374
HinP1I GCGC 3 cut(s) 17, 178, 374
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 134, 650, 913
HpaII CCGG 5 cut(s) 225, 351, 560, 565, 700
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 2 cut(s) 454, 907
Hpy188I TCNGA 6 cut(s) 121, 239, 308, 658, 991, 1009
Hpy188III TCNNGA 2 cut(s) 201, 886
Hpy8I GTNNAC 2 cut(s) 454, 907
Hpy99I CGWCG 2 cut(s) 257, 281
HpyAV CCTTC 1 cut(s) 644
HpyCH4III ACNGT 3 cut(s) 212, 451, 772
HpyCH4IV ACGT 2 cut(s) 101, 676
HpyCH4V TGCA 5 cut(s) 411, 416, 664, 721, 932
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 517, 526, 727, 827
HpyF3I CTNAG 4 cut(s) 420, 521, 940, 988
HpySE526I ACGT 2 cut(s) 101, 676
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 7 cut(s) 388, 502, 641, 721, 790, 889, 913
HspAI GCGC 3 cut(s) 17, 178, 374
Ksp22I TGATCA 1 cut(s) 1012
Kzo9I GATC 3 cut(s) 340, 364, 1012
LmnI GCTCC 3 cut(s) 24, 62, 578
Lsp1109I GCAGC 4 cut(s) 593, 611, 676, 968
MaeI CTAG 2 cut(s) 705, 855
MaeII ACGT 2 cut(s) 101, 676
MaeIII GTNAC 3 cut(s) 212, 695, 844
MalI GATC 3 cut(s) 342, 366, 1014
MboI GATC 3 cut(s) 340, 364, 1012
MboII GAAGA 3 cut(s) 290, 935, 1020
MfeI CAATTG 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 7 cut(s) 122, 258, 286, 748, 812, 878, 950
MluNI TGGCCA 1 cut(s) 529
MlyI GAGTC 2 cut(s) 128, 659
MnlI CCTC 8 cut(s) 20, 23, 63, 339, 457, 664, 748, 997
Mox20I TGGCCA 1 cut(s) 529
MroXI GAANNNNTTC 1 cut(s) 881
MscI TGGCCA 1 cut(s) 529
MseI TTAA 4 cut(s) 6, 285, 471, 594
Msp20I TGGCCA 1 cut(s) 529
MspA1I CMGCKG 1 cut(s) 584
MspCI CTTAAG 1 cut(s) 470
MspI CCGG 5 cut(s) 225, 351, 560, 565, 700
MspR9I CCNGG 2 cut(s) 226, 566
MunI CAATTG 1 cut(s) 258
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 184, 517, 526, 727, 827
NciI CCSGG 2 cut(s) 226, 566
NdeII GATC 3 cut(s) 340, 364, 1012
NlaIII CATG 7 cut(s) 388, 502, 641, 721, 790, 889, 913
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 1 cut(s) 844
NsbI TGCGCA 1 cut(s) 375
PagI TCATGA 1 cut(s) 885
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 1 cut(s) 913
Pfl23II CGTACG 2 cut(s) 102, 446
PkrI GCNGC 5 cut(s) 182, 583, 626, 666, 958
PleI GAGTC 2 cut(s) 128, 658
PpsI GAGTC 2 cut(s) 128, 658
Ppu21I YACGTR 2 cut(s) 102, 677
PpuMI RGGWCCY 2 cut(s) 10, 962
PsiI TTATAA 1 cut(s) 969
Psp5II RGGWCCY 2 cut(s) 10, 962
PspEI GGTNACC 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 160
PspLI CGTACG 2 cut(s) 102, 446
PspPI GGNCC 4 cut(s) 10, 562, 714, 962
PspPPI RGGWCCY 2 cut(s) 10, 962
PstNI CAGNNNCTG 2 cut(s) 727, 836
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 4 cut(s) 104, 425, 448, 859
RsaNI GTAC 4 cut(s) 103, 424, 447, 858
Rsr2I CGGWCCG 1 cut(s) 562
RsrII CGGWCCG 1 cut(s) 562
SaqAI TTAA 4 cut(s) 6, 285, 471, 594
SatI GCNGC 5 cut(s) 181, 582, 625, 665, 957
Sau3AI GATC 3 cut(s) 340, 364, 1012
Sau96I GGNCC 4 cut(s) 10, 562, 714, 962
SchI GAGTC 2 cut(s) 128, 659
ScrFI CCNGG 2 cut(s) 226, 566
SinI GGWCC 3 cut(s) 10, 562, 962
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
SpeI ACTAGT 1 cut(s) 854
Sse9I AATT 7 cut(s) 122, 258, 286, 748, 812, 878, 950
SsiI CCGC 4 cut(s) 168, 181, 185, 1000
SspMI CTAG 2 cut(s) 705, 855
StyD4I CCNGG 2 cut(s) 224, 564
TaaI ACNGT 3 cut(s) 212, 451, 772
TaiI ACGT 2 cut(s) 104, 679
TaqI TCGA 1 cut(s) 319
TasI AATT 7 cut(s) 122, 258, 286, 748, 812, 878, 950
TatI WGTACW 2 cut(s) 423, 857
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 913
Tru1I TTAA 4 cut(s) 6, 285, 471, 594
Tru9I TTAA 4 cut(s) 6, 285, 471, 594
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 4 cut(s) 581, 624, 664, 956
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 7 cut(s) 96, 401, 626, 803, 874, 902, 936
TspGWI ACGGA 4 cut(s) 39, 75, 89, 333
TspRI CASTG 1 cut(s) 775
Vha464I CTTAAG 1 cut(s) 470
VpaK11BI GGWCC 3 cut(s) 10, 562, 962
XapI RAATTY 1 cut(s) 748
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 2 cut(s) 705, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.