Rh6BG360800

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011175
Physical Location & Seq
Forward (+)
59121507 .. 59124013
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG360800.1

Sequence Viewer

Length: 1017 bp
ATGATTAAGGTCCTCGCGCTCGCTAACCCCTCTCTATACGGAGCTGAATGGACGGACGATGAATACTCCTATTCCCACCCGTACGGCTTATCATCATCGGAATTGTATATGGACTCTTTACTGGGCTTCCCACAATCCCCAGCTACCGCCATCTTGCGCCGCCCGCTAATCTCCACTTCAAGACCAAACAGTAACAGAAATGCCGGGTTGAGTTTCAGAGCAAAACCCAACGACGCAATTGACCCATTACTCCACGACGCCGTTAATTCAGCTTCTCTTCGTTTCCGAGAGACCCATCGACCAGAGCCTCTCTTTGTTGATCCGTATGCCGGTTGCTTTGTTGATCCTAATGCGCAGATGGACATGAAGCAATGCTCACACCATTATTGCGTTGCAACTAAGTACATTGATGATAAGTTGCTTCGTACAGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACTCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATTATTTTTGATATTTCGCCGGACCGGGTATTCAATGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGCTGCTTATTCCTTCATGTTCCATTGGAGTCCTCCGATTTGCAGCAAACTCTACGTGCTAAAGGCTTCAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCCGTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTAAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGACTAGTACAAGGGAATGGATGGAGAAATTATTCATGAACAATGGCTTTCGGGTGGACATGATTCACTATGAAGAAGTTGCAAAGAGCTTAGGCAAGAAATTAGCAGCAGGACCTTATAAGAATATACTATTTTCCTCAGAACAACTGCGGTTTTCTGATGATCAGGTATGGTGTTCCTCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

338

Amino Acids

37.83

Weight (kDa)

6.25

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 87 - 236 3.9e-24 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
Acc16I TGCGCA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 445
AccII CGCG 1 cut(s) 17
AciI CCGC 4 cut(s) 147, 160, 164, 979
AclWI GGATC 2 cut(s) 314, 338
AcoI YGGCCR 2 cut(s) 488, 506
AcsI RAATTY 1 cut(s) 727
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 4 cut(s) 83, 404, 427, 838
AfiI CCNNNNNNNGG 2 cut(s) 329, 442
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 4 cut(s) 180, 516, 553, 670
AhlI ACTAGT 1 cut(s) 833
AluBI AGCT 7 cut(s) 44, 143, 272, 563, 571, 603, 918
AluI AGCT 7 cut(s) 44, 143, 272, 563, 571, 603, 918
Alw26I GTCTC 2 cut(s) 284, 806
AlwI GGATC 2 cut(s) 314, 338
AlwNI CAGNNNCTG 2 cut(s) 706, 815
AoxI GGCC 4 cut(s) 488, 506, 680, 693
ApeKI GCWGC 4 cut(s) 560, 603, 643, 935
ApoI RAATTY 1 cut(s) 727
Asp700I GAANNNNTTC 1 cut(s) 860
AspLEI GCGC 3 cut(s) 19, 159, 355
AspS9I GGNCC 4 cut(s) 10, 541, 693, 941
AsuC2I CCSGG 2 cut(s) 205, 545
AvaII GGWCC 3 cut(s) 10, 541, 941
BalI TGGCCA 1 cut(s) 508
BbvI GCAGC 4 cut(s) 572, 590, 655, 947
BccI CCATC 5 cut(s) 158, 303, 352, 467, 844
BceAI ACGGC 3 cut(s) 100, 245, 475
BclI TGATCA 1 cut(s) 991
BcnI CCSGG 2 cut(s) 205, 545
BcoDI GTCTC 2 cut(s) 284, 806
BcuI ACTAGT 1 cut(s) 833
BfaI CTAG 2 cut(s) 684, 834
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 445
BglI GCCNNNNNGGC 1 cut(s) 496
BisI GCNGC 5 cut(s) 160, 561, 604, 644, 936
BlsI GCNGC 5 cut(s) 161, 562, 605, 645, 937
Bme1390I CCNGG 2 cut(s) 205, 545
Bme18I GGWCC 3 cut(s) 10, 541, 941
BmgT120I GGNCC 4 cut(s) 10, 541, 693, 941
BmrFI CCNGG 2 cut(s) 205, 545
BmrI ACTGGG 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 919
BpuMI CCSGG 2 cut(s) 205, 545
BsaAI YACGTR 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 442
Bse118I RCCGGY 2 cut(s) 329, 678
Bse1I ACTGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 377
BseGI GGATG 2 cut(s) 779, 855
BseLI CCNNNNNNNGG 2 cut(s) 329, 442
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 981
BseNI ACTGG 1 cut(s) 126
BseX3I CGGCCG 1 cut(s) 488
BseXI GCAGC 4 cut(s) 572, 590, 655, 947
BseYI CCCAGC 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 491
BshFI GGCC 4 cut(s) 490, 508, 682, 695
BsiEI CGRYCG 1 cut(s) 491
BsiSI CCGG 5 cut(s) 204, 330, 539, 544, 679
BsiWI CGTACG 1 cut(s) 81
BslFI GGGAC 2 cut(s) 459, 717
BslI CCNNNNNNNGG 2 cut(s) 329, 442
BsmAI GTCTC 2 cut(s) 284, 806
BsmFI GGGAC 2 cut(s) 459, 717
BsnI GGCC 4 cut(s) 490, 508, 682, 695
Bso31I GGTCTC 1 cut(s) 284
Bsp143I GATC 3 cut(s) 319, 343, 991
BspACI CCGC 4 cut(s) 147, 160, 164, 979
BspANI GGCC 4 cut(s) 490, 508, 682, 695
BspCNI CTCAG 1 cut(s) 980
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 864
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 2 cut(s) 314, 338
BspTI CTTAAG 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 377
BsrFI RCCGGY 2 cut(s) 329, 678
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 2 cut(s) 329, 678
BssMI GATC 3 cut(s) 319, 343, 991
BssNI GRCGYC 1 cut(s) 258
Bst4CI ACNGT 3 cut(s) 191, 430, 751
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 1 cut(s) 258
BstAFI CTTAAG 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 706
BstBAI YACGTR 1 cut(s) 656
BstC8I GCNNGC 3 cut(s) 21, 164, 798
BstDEI CTNAG 5 cut(s) 399, 500, 919, 967, 1009
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 2 cut(s) 779, 855
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 3 cut(s) 19, 159, 355
BstKTI GATC 3 cut(s) 322, 346, 994
BstMAI GTCTC 2 cut(s) 284, 806
BstMBI GATC 3 cut(s) 319, 343, 991
BstMCI CGRYCG 1 cut(s) 491
BstMWI GCNNNNNNNGC 5 cut(s) 163, 496, 505, 706, 806
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 2 cut(s) 203, 543
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 572, 590, 655, 947
BstZI CGGCCG 1 cut(s) 488
BsuRI GGCC 4 cut(s) 490, 508, 682, 695
BtsCI GGATG 2 cut(s) 779, 855
BtsIMutI CAGTG 2 cut(s) 435, 747
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 21, 164, 798
CaiI CAGNNNCTG 2 cut(s) 706, 815
CciI TCATGA 1 cut(s) 864
CfoI GCGC 3 cut(s) 19, 159, 355
Cfr10I RCCGGY 2 cut(s) 329, 678
Cfr13I GGNCC 4 cut(s) 10, 541, 693, 941
CpoI CGGWCCG 1 cut(s) 541
CseI GACGC 2 cut(s) 242, 266
Csp6I GTAC 4 cut(s) 82, 403, 426, 837
CspI CGGWCCG 1 cut(s) 541
CviAII CATG 7 cut(s) 364, 478, 617, 697, 766, 865, 889
CviQI GTAC 4 cut(s) 82, 403, 426, 837
DdeI CTNAG 5 cut(s) 399, 500, 919, 967, 1009
DpnI GATC 3 cut(s) 321, 345, 993
DpnII GATC 3 cut(s) 319, 343, 991
EaeI YGGCCR 2 cut(s) 488, 506
EagI CGGCCG 1 cut(s) 488
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
EclXI CGGCCG 1 cut(s) 488
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 10, 541, 941
Eco52I CGGCCG 1 cut(s) 488
Eco91I GGTNACC 1 cut(s) 674
EcoO109I RGGNCCY 2 cut(s) 10, 941
EcoO65I GGTNACC 1 cut(s) 674
FaeI CATG 7 cut(s) 367, 481, 620, 700, 769, 868, 892
FaqI GGGAC 2 cut(s) 459, 717
FatI CATG 7 cut(s) 363, 477, 616, 696, 765, 864, 888
FauI CCCGC 1 cut(s) 171
FbaI TGATCA 1 cut(s) 991
Fnu4HI GCNGC 5 cut(s) 160, 561, 604, 644, 936
FokI GGATG 2 cut(s) 786, 862
Fsp4HI GCNGC 5 cut(s) 160, 561, 604, 644, 936
FspBI CTAG 2 cut(s) 684, 834
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 3 cut(s) 18, 158, 354
GluI GCNGC 5 cut(s) 160, 561, 604, 644, 936
GsaI CCCAGC 1 cut(s) 143
HaeIII GGCC 4 cut(s) 490, 508, 682, 695
HapII CCGG 5 cut(s) 204, 330, 539, 544, 679
HgaI GACGC 2 cut(s) 242, 266
HhaI GCGC 3 cut(s) 19, 159, 355
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 7 cut(s) 367, 481, 620, 700, 769, 868, 892
Hin6I GCGC 3 cut(s) 17, 157, 353
HinP1I GCGC 3 cut(s) 17, 157, 353
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 3 cut(s) 113, 629, 892
HpaII CCGG 5 cut(s) 204, 330, 539, 544, 679
Hpy166II GTNNAC 2 cut(s) 433, 886
Hpy188I TCNGA 6 cut(s) 100, 218, 287, 637, 970, 988
Hpy188III TCNNGA 2 cut(s) 180, 865
Hpy8I GTNNAC 2 cut(s) 433, 886
Hpy99I CGWCG 2 cut(s) 236, 260
HpyAV CCTTC 1 cut(s) 623
HpyCH4III ACNGT 3 cut(s) 191, 430, 751
HpyCH4IV ACGT 1 cut(s) 655
HpyCH4V TGCA 4 cut(s) 395, 643, 700, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 163, 496, 505, 706, 806
HpyF3I CTNAG 5 cut(s) 399, 500, 919, 967, 1009
HpySE526I ACGT 1 cut(s) 655
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 7 cut(s) 367, 481, 620, 700, 769, 868, 892
HspAI GCGC 3 cut(s) 17, 157, 353
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 3 cut(s) 319, 343, 991
LmnI GCTCC 2 cut(s) 41, 557
Lsp1109I GCAGC 4 cut(s) 572, 590, 655, 947
MaeI CTAG 2 cut(s) 684, 834
MaeII ACGT 1 cut(s) 655
MaeIII GTNAC 3 cut(s) 191, 674, 823
MalI GATC 3 cut(s) 321, 345, 993
MboI GATC 3 cut(s) 319, 343, 991
MboII GAAGA 2 cut(s) 269, 914
MfeI CAATTG 1 cut(s) 237
MlsI TGGCCA 1 cut(s) 508
MluCI AATT 7 cut(s) 101, 237, 265, 727, 791, 857, 929
MluNI TGGCCA 1 cut(s) 508
MlyI GAGTC 2 cut(s) 107, 638
MnlI CCTC 7 cut(s) 23, 40, 318, 436, 643, 727, 976
Mox20I TGGCCA 1 cut(s) 508
MroXI GAANNNNTTC 1 cut(s) 860
MscI TGGCCA 1 cut(s) 508
MseI TTAA 4 cut(s) 6, 264, 450, 573
Msp20I TGGCCA 1 cut(s) 508
MspA1I CMGCKG 1 cut(s) 563
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 5 cut(s) 204, 330, 539, 544, 679
MspR9I CCNGG 2 cut(s) 205, 545
MunI CAATTG 1 cut(s) 237
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 163, 496, 505, 706, 806
NciI CCSGG 2 cut(s) 205, 545
NdeII GATC 3 cut(s) 319, 343, 991
NlaIII CATG 7 cut(s) 367, 481, 620, 700, 769, 868, 892
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 823
NsbI TGCGCA 1 cut(s) 354
PagI TCATGA 1 cut(s) 864
PdmI GAANNNNTTC 1 cut(s) 860
PfeI GAWTC 1 cut(s) 892
Pfl23II CGTACG 1 cut(s) 81
PkrI GCNGC 5 cut(s) 161, 562, 605, 645, 937
PleI GAGTC 2 cut(s) 107, 637
PpsI GAGTC 2 cut(s) 107, 637
Ppu21I YACGTR 1 cut(s) 656
PpuMI RGGWCCY 2 cut(s) 10, 941
PsiI TTATAA 1 cut(s) 948
Psp5II RGGWCCY 2 cut(s) 10, 941
PspEI GGTNACC 1 cut(s) 674
PspFI CCCAGC 1 cut(s) 139
PspLI CGTACG 1 cut(s) 81
PspPI GGNCC 4 cut(s) 10, 541, 693, 941
PspPPI RGGWCCY 2 cut(s) 10, 941
PstNI CAGNNNCTG 2 cut(s) 706, 815
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 4 cut(s) 83, 404, 427, 838
RsaNI GTAC 4 cut(s) 82, 403, 426, 837
Rsr2I CGGWCCG 1 cut(s) 541
RsrII CGGWCCG 1 cut(s) 541
SaqAI TTAA 4 cut(s) 6, 264, 450, 573
SatI GCNGC 5 cut(s) 160, 561, 604, 644, 936
Sau3AI GATC 3 cut(s) 319, 343, 991
Sau96I GGNCC 4 cut(s) 10, 541, 693, 941
SchI GAGTC 2 cut(s) 107, 638
ScrFI CCNGG 2 cut(s) 205, 545
SinI GGWCC 3 cut(s) 10, 541, 941
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SpeI ACTAGT 1 cut(s) 833
Sse9I AATT 7 cut(s) 101, 237, 265, 727, 791, 857, 929
SsiI CCGC 4 cut(s) 147, 160, 164, 979
SspMI CTAG 2 cut(s) 684, 834
StyD4I CCNGG 2 cut(s) 203, 543
TaaI ACNGT 3 cut(s) 191, 430, 751
TaiI ACGT 1 cut(s) 658
TaqI TCGA 1 cut(s) 298
TasI AATT 7 cut(s) 101, 237, 265, 727, 791, 857, 929
TatI WGTACW 2 cut(s) 402, 836
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 1 cut(s) 892
Tru1I TTAA 4 cut(s) 6, 264, 450, 573
Tru9I TTAA 4 cut(s) 6, 264, 450, 573
TscAI CASTG 2 cut(s) 435, 754
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 4 cut(s) 560, 603, 643, 935
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 7 cut(s) 75, 380, 605, 782, 853, 881, 915
TspGWI ACGGA 3 cut(s) 54, 68, 312
TspRI CASTG 2 cut(s) 435, 754
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 3 cut(s) 10, 541, 941
XapI RAATTY 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 860
XspI CTAG 2 cut(s) 684, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.