FvH4_2g25800

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011175
Physical Location & Seq
Forward (+)
20826062 .. 20829226
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25800.t1

Sequence Viewer

Length: 954 bp
ATGGACTCTTTGCTGGGTTTCGCACGCTCCCCAGCTACTGCTTTCTTGCGCCGTCCCCTGACCTACACTTCAACACCAATCAGAAATGGCGGGCTGAGCTTCCGAGCAAAACCCAACGACGAAATTGACCCACTACTCCATGACGCCATCCATTCAGCCTCTCTTCGTTTCCGAGAGACCCATCGACCAGAGCCTCTGTTTGTTGATCCATATGCGGGCTGCTTTGTTCATCCCAATGCGCAGATGGATATGAAGCAATGCTCACACCATTACTGCCTTGCTACTAAGTACATTGATGATAAGTTGCTTCGTACAGTGAATCATATTGATGGACTCAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACTCGGCCATATAGGCTTAATTGGCCGACTTCAACCATAATATTTGATATATCGCCGGACCGGGTATTCAGTGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGTTGCTTATTCCTTCATGTTCCATTGGAGTCCTCCGACTTGCAACAAACTCTACGTGCTAAAGGCTTTAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCTGTGATGACCTTGGCAAATTTTGAGGATATTTTGCTAACCGTAAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGTGAATTGCCGGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGGCTAGTGCAAGGGAATGGATAGAGAAGTTATTCATGAACAATGGCTTTCGGGTGGACATGATTCGCTATGAAGAAGTCGCAAAGAGTTTAGGCAAAGAATTAGCAGCAGGACCTTATAAGAATATACTATTTTCCTCAGAACAACTGCGGTTTTCTGATGATCAGATGGAAACATGGAGAAGAGAATTTCAACGAGTTGAGGAACAAGGGGATGAAGAAGGTTTTGAAGAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

318

Amino Acids

35.92

Weight (kDa)

5.81

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 49 - 198 1.1e-23 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
Acc16I TGCGCA 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 331
AciI CCGC 3 cut(s) 90, 215, 865
AclWI GGATC 1 cut(s) 200
AcoI YGGCCR 2 cut(s) 374, 392
AcsI RAATTY 2 cut(s) 613, 902
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 2 cut(s) 290, 313
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 4 cut(s) 72, 402, 908, 944
AluBI AGCT 5 cut(s) 35, 99, 449, 457, 949
AluI AGCT 5 cut(s) 35, 99, 449, 457, 949
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 170, 692
AlwI GGATC 1 cut(s) 200
AlwNI CAGNNNCTG 3 cut(s) 38, 592, 701
AoxI GGCC 4 cut(s) 374, 392, 566, 579
ApeKI GCWGC 3 cut(s) 219, 446, 821
ApoI RAATTY 2 cut(s) 613, 902
Asp700I GAANNNNTTC 1 cut(s) 746
AspLEI GCGC 2 cut(s) 51, 241
AspS9I GGNCC 3 cut(s) 427, 579, 827
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 686
AvaII GGWCC 2 cut(s) 427, 827
BanII GRGCYC 1 cut(s) 951
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 3 cut(s) 206, 458, 833
BccI CCATC 6 cut(s) 155, 189, 238, 323, 353, 877
BceAI ACGGC 1 cut(s) 36
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 2 cut(s) 170, 692
BfaI CTAG 2 cut(s) 570, 720
BfuAI ACCTGC 1 cut(s) 331
BglI GCCNNNNNGGC 1 cut(s) 382
BisI GCNGC 3 cut(s) 220, 447, 822
BlpI GCTNAGC 1 cut(s) 95
BlsI GCNGC 3 cut(s) 221, 448, 823
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 2 cut(s) 427, 827
BmgT120I GGNCC 3 cut(s) 427, 579, 827
BmrFI CCNGG 1 cut(s) 431
Bpu1102I GCTNAGC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 431
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 606, 714
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse118I RCCGGY 2 cut(s) 564, 682
Bse3DI GCAATG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 606, 714
BseGI GGATG 4 cut(s) 147, 229, 665, 934
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 86, 867
BseXI GCAGC 3 cut(s) 206, 458, 833
BseYI CCCAGC 2 cut(s) 13, 31
BshFI GGCC 4 cut(s) 376, 394, 568, 581
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 4 cut(s) 425, 430, 565, 683
BslFI GGGAC 2 cut(s) 39, 603
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 2 cut(s) 170, 692
BsmFI GGGAC 2 cut(s) 39, 603
BsnI GGCC 4 cut(s) 376, 394, 568, 581
Bso31I GGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 951
Bsp143I GATC 2 cut(s) 205, 877
Bsp1720I GCTNAGC 1 cut(s) 95
BspACI CCGC 3 cut(s) 90, 215, 865
BspANI GGCC 4 cut(s) 376, 394, 568, 581
BspCNI CTCAG 2 cut(s) 87, 866
BspHI TCATGA 1 cut(s) 750
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 939
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 263
BsrFI RCCGGY 2 cut(s) 564, 682
BssAI RCCGGY 2 cut(s) 564, 682
BssECI CCNNGG 2 cut(s) 606, 714
BssMI GATC 2 cut(s) 205, 877
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 2 cut(s) 606, 714
Bst4CI ACNGT 2 cut(s) 316, 637
Bst6I CTCTTC 3 cut(s) 168, 892, 939
BstACI GRCGYC 1 cut(s) 144
BstAPI GCANNNNNTGC 1 cut(s) 592
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 25, 92, 217, 684
BstDEI CTNAG 3 cut(s) 95, 285, 853
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 4 cut(s) 147, 229, 665, 934
BstHHI GCGC 2 cut(s) 51, 241
BstKTI GATC 2 cut(s) 208, 880
BstMAI GTCTC 2 cut(s) 170, 692
BstMBI GATC 2 cut(s) 205, 877
BstMWI GCNNNNNNNGC 5 cut(s) 96, 382, 391, 592, 692
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 1 cut(s) 429
BstV1I GCAGC 3 cut(s) 206, 458, 833
BsuRI GGCC 4 cut(s) 376, 394, 568, 581
BtsCI GGATG 4 cut(s) 147, 229, 665, 934
BtsIMutI CAGTG 2 cut(s) 321, 445
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 4 cut(s) 25, 92, 217, 684
CaiI CAGNNNCTG 3 cut(s) 38, 592, 701
CciI TCATGA 1 cut(s) 750
CfoI GCGC 2 cut(s) 51, 241
Cfr10I RCCGGY 2 cut(s) 564, 682
Cfr13I GGNCC 3 cut(s) 427, 579, 827
CpoI CGGWCCG 1 cut(s) 427
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 289, 312
CspI CGGWCCG 1 cut(s) 427
CviAII CATG 8 cut(s) 140, 364, 503, 583, 652, 751, 775, 891
CviQI GTAC 2 cut(s) 289, 312
DdeI CTNAG 3 cut(s) 95, 285, 853
DpnI GATC 2 cut(s) 207, 879
DpnII GATC 2 cut(s) 205, 877
EaeI YGGCCR 2 cut(s) 374, 392
Eam1104I CTCTTC 3 cut(s) 168, 892, 939
EarI CTCTTC 3 cut(s) 168, 892, 939
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 2 cut(s) 606, 714
Eco24I GRGCYC 1 cut(s) 951
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 427, 827
Eco53kI GAGCTC 1 cut(s) 949
Eco91I GGTNACC 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 949
EcoO109I RGGNCCY 1 cut(s) 827
EcoO65I GGTNACC 1 cut(s) 560
EcoT14I CCWWGG 2 cut(s) 606, 714
EcoT38I GRGCYC 1 cut(s) 951
ErhI CCWWGG 2 cut(s) 606, 714
FaeI CATG 8 cut(s) 143, 367, 506, 586, 655, 754, 778, 894
FaqI GGGAC 2 cut(s) 39, 603
FatI CATG 8 cut(s) 139, 363, 502, 582, 651, 750, 774, 890
FauI CCCGC 2 cut(s) 83, 208
FauNDI CATATG 1 cut(s) 211
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 3 cut(s) 220, 447, 822
FokI GGATG 4 cut(s) 134, 216, 672, 941
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 3 cut(s) 220, 447, 822
FspBI CTAG 2 cut(s) 570, 720
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 2 cut(s) 50, 240
GluI GCNGC 3 cut(s) 220, 447, 822
GsaI CCCAGC 2 cut(s) 17, 35
HaeIII GGCC 4 cut(s) 376, 394, 568, 581
HapII CCGG 4 cut(s) 425, 430, 565, 683
HgaI GACGC 1 cut(s) 152
HhaI GCGC 2 cut(s) 51, 241
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 8 cut(s) 143, 367, 506, 586, 655, 754, 778, 894
Hin6I GCGC 2 cut(s) 49, 239
HinP1I GCGC 2 cut(s) 49, 239
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 5, 319, 333, 515, 778
HpaII CCGG 4 cut(s) 425, 430, 565, 683
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 1 cut(s) 772
Hpy188I TCNGA 7 cut(s) 83, 104, 173, 523, 856, 874, 882
Hpy188III TCNNGA 1 cut(s) 751
Hpy8I GTNNAC 1 cut(s) 772
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 509, 929
HpyCH4III ACNGT 2 cut(s) 316, 637
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 3 cut(s) 529, 586, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 382, 391, 592, 692
HpyF3I CTNAG 3 cut(s) 95, 285, 853
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 8 cut(s) 143, 367, 506, 586, 655, 754, 778, 894
HspAI GCGC 2 cut(s) 49, 239
KroI GCCGGC 1 cut(s) 682
KroNI GCCGGC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 2 cut(s) 205, 877
LguI GCTCTTC 1 cut(s) 939
LmnI GCTCC 2 cut(s) 32, 443
Lsp1109I GCAGC 3 cut(s) 206, 458, 833
MaeI CTAG 2 cut(s) 570, 720
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 560, 709
MalI GATC 2 cut(s) 207, 879
MboI GATC 2 cut(s) 205, 877
MboII GAAGA 4 cut(s) 155, 800, 909, 944
MhlI GDGCHC 1 cut(s) 951
MluCI AATT 6 cut(s) 123, 388, 613, 677, 815, 902
MlyI GAGTC 2 cut(s) 327, 524
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 6 cut(s) 169, 204, 529, 613, 862, 910
MroNI GCCGGC 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 746
MseI TTAA 3 cut(s) 387, 459, 555
MslI CAYNNNNRTG 2 cut(s) 234, 327
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 4 cut(s) 425, 430, 565, 683
MspR9I CCNGG 1 cut(s) 431
MwoI GCNNNNNNNGC 5 cut(s) 96, 382, 391, 592, 692
NaeI GCCGGC 1 cut(s) 684
NciI CCSGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 211
NdeII GATC 2 cut(s) 205, 877
NgoMIV GCCGGC 1 cut(s) 682
NlaIII CATG 8 cut(s) 143, 367, 506, 586, 655, 754, 778, 894
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 1 cut(s) 709
NsbI TGCGCA 1 cut(s) 240
PagI TCATGA 1 cut(s) 750
PciSI GCTCTTC 1 cut(s) 939
PdiI GCCGGC 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 2 cut(s) 319, 778
PkrI GCNGC 3 cut(s) 221, 448, 823
PleI GAGTC 2 cut(s) 327, 523
PpsI GAGTC 2 cut(s) 327, 523
Ppu21I YACGTR 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 827
PsiI TTATAA 1 cut(s) 834
Psp124BI GAGCTC 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 827
PspEI GGTNACC 1 cut(s) 560
PspFI CCCAGC 2 cut(s) 13, 31
PspPI GGNCC 3 cut(s) 427, 579, 827
PspPPI RGGWCCY 1 cut(s) 827
PstNI CAGNNNCTG 3 cut(s) 38, 592, 701
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 2 cut(s) 290, 313
RsaNI GTAC 2 cut(s) 289, 312
RseI CAYNNNNRTG 2 cut(s) 234, 327
Rsr2I CGGWCCG 1 cut(s) 427
RsrII CGGWCCG 1 cut(s) 427
SacI GAGCTC 1 cut(s) 951
SapI GCTCTTC 1 cut(s) 939
SaqAI TTAA 3 cut(s) 387, 459, 555
SatI GCNGC 3 cut(s) 220, 447, 822
Sau3AI GATC 2 cut(s) 205, 877
Sau96I GGNCC 3 cut(s) 427, 579, 827
SchI GAGTC 2 cut(s) 327, 524
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 1 cut(s) 951
SinI GGWCC 2 cut(s) 427, 827
SmiMI CAYNNNNRTG 2 cut(s) 234, 327
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 6 cut(s) 123, 388, 613, 677, 815, 902
SsiI CCGC 3 cut(s) 90, 215, 865
SspI AATATT 1 cut(s) 411
SspMI CTAG 2 cut(s) 570, 720
SstI GAGCTC 1 cut(s) 951
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 2 cut(s) 606, 714
TaaI ACNGT 2 cut(s) 316, 637
TaiI ACGT 1 cut(s) 544
TaqI TCGA 1 cut(s) 184
TasI AATT 6 cut(s) 123, 388, 613, 677, 815, 902
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 2 cut(s) 319, 778
Tru1I TTAA 3 cut(s) 387, 459, 555
Tru9I TTAA 3 cut(s) 387, 459, 555
TscAI CASTG 2 cut(s) 321, 445
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 3 cut(s) 219, 446, 821
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 8 cut(s) 218, 266, 491, 668, 739, 767, 801, 945
TspRI CASTG 2 cut(s) 321, 445
VpaK11BI GGWCC 2 cut(s) 427, 827
XapI RAATTY 2 cut(s) 613, 902
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 746
XspI CTAG 2 cut(s) 570, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.