Rroxscaffold_7G00173640

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011175
Physical Location & Seq
Reverse (-)
13590144 .. 13592673
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173640.1

Sequence Viewer

Length: 1059 bp
ATGATTAAGGTCCTCGCGCTCTGTGGAGGGAAGCTGTCTGTGACTCTAACCCCCCTATACGGAGCTGAATGGACGGACGATGAATACTCCTATTCCCACTCGTACGGCTTATCATCATCGGAATTGTATATGGGCTCTTTACTGGGCTTCCCACAATCCCCAGCTACTGCCATCTTGCGCCGCCCGCTAGTCTCCACTTCAAGACCAAACAGAAATGCCGGGTTGAGTTTCAGAGCAAAACCCAACGACGCAATTGACCCATTACTCCACGACGCCGTTAATTCAGCTTCTCTTCAGCCTCTCTTTGTTGATCCGTATGCCGGTTGCTTTGTTGATCCTAATGCGCAGATGGACATGAAGCAATGCTCACACCATTATTGCATTGCAACTAAGTACATTGATGATAAGTTGCTTCGTACAGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACTCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATAATTTTTGATATTTCGCCGGACCGGGTATTCAGTGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGCTGCTTGTTCCTTCATGTTCCATTGGAGTCCTCCGATTTGCAGCAAACTCTACGTGCTAAAGGCTTCAATGGTAACCAGCCTAGTATATGGGCCATGCAGGGACTGCCCTTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTAAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGACTAGTACAAGGGAATGGATGGAGAAATTATTCATGAACAACGGCTTTCGGGTGGACATGATTCGCTATGAAGAAGTTGCAAAGAGTTTAGGCAAGAAATTAGCGGCGGCACCTTACAAGAATATACTATTTTCCTCAGAACAACTGCGGTTTTCTGATGATCAGATGGAAACTTGGAGAAGGGAATTTCAAAGAGTTGAGGAACAAGGGGATGAAGAAGGTTTCGAAGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

352

Amino Acids

39.41

Weight (kDa)

5.39

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 90 - 233 2.9e-22 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 931
AccII CGCG 1 cut(s) 17
AciI CCGC 5 cut(s) 181, 185, 926, 929, 970
AclWI GGATC 2 cut(s) 305, 329
AcoI YGGCCR 2 cut(s) 479, 497
AcsI RAATTY 2 cut(s) 718, 1007
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 4 cut(s) 104, 395, 418, 829
AfiI CCNNNNNNNGG 3 cut(s) 59, 320, 433
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 4 cut(s) 201, 507, 661, 1013
AhlI ACTAGT 1 cut(s) 824
AluBI AGCT 8 cut(s) 34, 65, 164, 287, 554, 562, 594, 1054
AluI AGCT 8 cut(s) 34, 65, 164, 287, 554, 562, 594, 1054
Alw26I GTCTC 2 cut(s) 196, 797
AlwI GGATC 2 cut(s) 305, 329
AlwNI CAGNNNCTG 3 cut(s) 167, 697, 806
AoxI GGCC 3 cut(s) 479, 497, 684
ApeKI GCWGC 3 cut(s) 551, 594, 634
ApoI RAATTY 2 cut(s) 718, 1007
Asp700I GAANNNNTTC 1 cut(s) 851
AspLEI GCGC 3 cut(s) 19, 180, 346
AspS9I GGNCC 3 cut(s) 10, 532, 684
AsuC2I CCSGG 2 cut(s) 220, 536
AsuII TTCGAA 1 cut(s) 1047
AvaII GGWCC 2 cut(s) 10, 532
BalI TGGCCA 1 cut(s) 499
BanI GGYRCC 1 cut(s) 931
BanII GRGCYC 1 cut(s) 137
BbvI GCAGC 3 cut(s) 563, 581, 646
BccI CCATC 5 cut(s) 179, 343, 458, 835, 982
BceAI ACGGC 4 cut(s) 121, 260, 466, 880
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 2 cut(s) 220, 536
BcoDI GTCTC 2 cut(s) 196, 797
BcuI ACTAGT 1 cut(s) 824
BfaI CTAG 3 cut(s) 188, 675, 825
BfrI CTTAAG 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 436
BglI GCCNNNNNGGC 1 cut(s) 487
BisI GCNGC 6 cut(s) 181, 552, 595, 635, 927, 930
BlsI GCNGC 6 cut(s) 182, 553, 596, 636, 928, 931
Bme1390I CCNGG 2 cut(s) 220, 536
Bme18I GGWCC 2 cut(s) 10, 532
BmgT120I GGNCC 3 cut(s) 10, 532, 684
BmiI GGNNCC 1 cut(s) 933
BmrFI CCNGG 2 cut(s) 220, 536
BmrI ACTGGG 1 cut(s) 152
BmuI ACTGGG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 1047
BpuMI CCSGG 2 cut(s) 220, 536
BsaAI YACGTR 1 cut(s) 647
BsaHI GRCGYC 1 cut(s) 273
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 320, 433
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 147
Bse3DI GCAATG 2 cut(s) 368, 381
BseGI GGATG 3 cut(s) 770, 846, 1039
BseLI CCNNNNNNNGG 3 cut(s) 59, 320, 433
BseMI GCAATG 2 cut(s) 368, 381
BseMII CTCAG 1 cut(s) 972
BseNI ACTGG 1 cut(s) 147
BseX3I CGGCCG 1 cut(s) 479
BseXI GCAGC 3 cut(s) 563, 581, 646
BseYI CCCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 3 cut(s) 481, 499, 686
BshNI GGYRCC 1 cut(s) 931
BsiEI CGRYCG 1 cut(s) 482
BsiSI CCGG 4 cut(s) 219, 321, 530, 535
BsiWI CGTACG 1 cut(s) 102
BslFI GGGAC 2 cut(s) 450, 708
BslI CCNNNNNNNGG 3 cut(s) 59, 320, 433
BsmAI GTCTC 2 cut(s) 196, 797
BsmFI GGGAC 2 cut(s) 450, 708
BsnI GGCC 3 cut(s) 481, 499, 686
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 1 cut(s) 137
Bsp143I GATC 3 cut(s) 310, 334, 982
BspACI CCGC 5 cut(s) 181, 185, 926, 929, 970
BspANI GGCC 3 cut(s) 481, 499, 686
BspCNI CTCAG 1 cut(s) 971
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 1 cut(s) 933
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 2 cut(s) 305, 329
BspQI GCTCTTC 1 cut(s) 1044
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 1 cut(s) 931
BspTI CTTAAG 1 cut(s) 440
BsrDI GCAATG 2 cut(s) 368, 381
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 147
BssAI RCCGGY 1 cut(s) 320
BssMI GATC 3 cut(s) 310, 334, 982
BssNI GRCGYC 1 cut(s) 273
Bst4CI ACNGT 2 cut(s) 421, 742
Bst6I CTCTTC 2 cut(s) 297, 1044
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 697
BstBAI YACGTR 1 cut(s) 647
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 185, 789
BstDEI CTNAG 3 cut(s) 390, 491, 958
BstEII GGTNACC 1 cut(s) 665
BstF5I GGATG 3 cut(s) 770, 846, 1039
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 3 cut(s) 19, 180, 346
BstKTI GATC 3 cut(s) 313, 337, 985
BstMAI GTCTC 2 cut(s) 196, 797
BstMBI GATC 3 cut(s) 310, 334, 982
BstMCI CGRYCG 1 cut(s) 482
BstMWI GCNNNNNNNGC 5 cut(s) 184, 487, 496, 697, 797
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 2 cut(s) 218, 534
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 3 cut(s) 563, 581, 646
BstZI CGGCCG 1 cut(s) 479
BsuRI GGCC 3 cut(s) 481, 499, 686
BtsCI GGATG 3 cut(s) 770, 846, 1039
BtsIMutI CAGTG 3 cut(s) 426, 550, 738
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 185, 789
CaiI CAGNNNCTG 3 cut(s) 167, 697, 806
CciI TCATGA 1 cut(s) 855
CfoI GCGC 3 cut(s) 19, 180, 346
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 10, 532, 684
CpoI CGGWCCG 1 cut(s) 532
CseI GACGC 2 cut(s) 257, 281
Csp6I GTAC 4 cut(s) 103, 394, 417, 828
CspI CGGWCCG 1 cut(s) 532
CviAII CATG 7 cut(s) 355, 469, 608, 688, 757, 856, 880
CviQI GTAC 4 cut(s) 103, 394, 417, 828
DdeI CTNAG 3 cut(s) 390, 491, 958
DpnI GATC 3 cut(s) 312, 336, 984
DpnII GATC 3 cut(s) 310, 334, 982
EaeI YGGCCR 2 cut(s) 479, 497
EagI CGGCCG 1 cut(s) 479
Eam1104I CTCTTC 2 cut(s) 297, 1044
EarI CTCTTC 2 cut(s) 297, 1044
EclXI CGGCCG 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 10, 532
Eco52I CGGCCG 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 278
Eco91I GGTNACC 1 cut(s) 665
EcoO109I RGGNCCY 1 cut(s) 10
EcoO65I GGTNACC 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 7 cut(s) 358, 472, 611, 691, 760, 859, 883
FaqI GGGAC 2 cut(s) 450, 708
FatI CATG 7 cut(s) 354, 468, 607, 687, 756, 855, 879
FauI CCCGC 1 cut(s) 192
FbaI TGATCA 1 cut(s) 982
Fnu4HI GCNGC 6 cut(s) 181, 552, 595, 635, 927, 930
FokI GGATG 3 cut(s) 777, 853, 1046
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 6 cut(s) 181, 552, 595, 635, 927, 930
FspBI CTAG 3 cut(s) 188, 675, 825
FspI TGCGCA 1 cut(s) 345
GlaI GCGC 3 cut(s) 18, 179, 345
GluI GCNGC 6 cut(s) 181, 552, 595, 635, 927, 930
GsaI CCCAGC 1 cut(s) 164
HaeIII GGCC 3 cut(s) 481, 499, 686
HapII CCGG 4 cut(s) 219, 321, 530, 535
HgaI GACGC 2 cut(s) 257, 281
HhaI GCGC 3 cut(s) 19, 180, 346
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 7 cut(s) 358, 472, 611, 691, 760, 859, 883
Hin6I GCGC 3 cut(s) 17, 178, 344
HinP1I GCGC 3 cut(s) 17, 178, 344
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 3 cut(s) 43, 620, 883
HpaII CCGG 4 cut(s) 219, 321, 530, 535
Hpy166II GTNNAC 2 cut(s) 424, 877
Hpy188I TCNGA 6 cut(s) 121, 233, 628, 961, 979, 987
Hpy188III TCNNGA 2 cut(s) 201, 856
Hpy8I GTNNAC 2 cut(s) 424, 877
Hpy99I CGWCG 2 cut(s) 251, 275
HpyAV CCTTC 3 cut(s) 614, 996, 1034
HpyCH4III ACNGT 2 cut(s) 421, 742
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 5 cut(s) 381, 386, 634, 691, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 487, 496, 697, 797
HpyF3I CTNAG 3 cut(s) 390, 491, 958
HpySE526I ACGT 1 cut(s) 646
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 7 cut(s) 358, 472, 611, 691, 760, 859, 883
HspAI GCGC 3 cut(s) 17, 178, 344
Ksp22I TGATCA 1 cut(s) 982
Kzo9I GATC 3 cut(s) 310, 334, 982
LguI GCTCTTC 1 cut(s) 1044
LmnI GCTCC 2 cut(s) 62, 548
Lsp1109I GCAGC 3 cut(s) 563, 581, 646
MaeI CTAG 3 cut(s) 188, 675, 825
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 3 cut(s) 40, 665, 814
MalI GATC 3 cut(s) 312, 336, 984
MboI GATC 3 cut(s) 310, 334, 982
MboII GAAGA 3 cut(s) 284, 905, 1049
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 137
MlsI TGGCCA 1 cut(s) 499
MluCI AATT 9 cut(s) 122, 252, 280, 513, 718, 782, 848, 920, 1007
MluNI TGGCCA 1 cut(s) 499
MlyI GAGTC 2 cut(s) 37, 629
MnlI CCTC 8 cut(s) 20, 23, 309, 427, 634, 718, 967, 1015
Mox20I TGGCCA 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 851
MscI TGGCCA 1 cut(s) 499
MseI TTAA 5 cut(s) 6, 279, 441, 564, 704
Msp20I TGGCCA 1 cut(s) 499
MspA1I CMGCKG 1 cut(s) 554
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 4 cut(s) 219, 321, 530, 535
MspR9I CCNGG 2 cut(s) 220, 536
MunI CAATTG 1 cut(s) 252
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 184, 487, 496, 697, 797
NciI CCSGG 2 cut(s) 220, 536
NdeII GATC 3 cut(s) 310, 334, 982
NlaIII CATG 7 cut(s) 358, 472, 611, 691, 760, 859, 883
NlaIV GGNNCC 1 cut(s) 933
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 2 cut(s) 40, 814
NsbI TGCGCA 1 cut(s) 345
NspV TTCGAA 1 cut(s) 1047
PagI TCATGA 1 cut(s) 855
PciSI GCTCTTC 1 cut(s) 1044
PdmI GAANNNNTTC 1 cut(s) 851
PfeI GAWTC 1 cut(s) 883
Pfl23II CGTACG 1 cut(s) 102
PkrI GCNGC 6 cut(s) 182, 553, 596, 636, 928, 931
PleI GAGTC 2 cut(s) 37, 628
PpsI GAGTC 2 cut(s) 37, 628
Ppu21I YACGTR 1 cut(s) 647
PpuMI RGGWCCY 1 cut(s) 10
Psp5II RGGWCCY 1 cut(s) 10
PspEI GGTNACC 1 cut(s) 665
PspFI CCCAGC 1 cut(s) 160
PspLI CGTACG 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 933
PspPI GGNCC 3 cut(s) 10, 532, 684
PspPPI RGGWCCY 1 cut(s) 10
PstNI CAGNNNCTG 3 cut(s) 167, 697, 806
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 4 cut(s) 104, 395, 418, 829
RsaNI GTAC 4 cut(s) 103, 394, 417, 828
Rsr2I CGGWCCG 1 cut(s) 532
RsrII CGGWCCG 1 cut(s) 532
SapI GCTCTTC 1 cut(s) 1044
SaqAI TTAA 5 cut(s) 6, 279, 441, 564, 704
SatI GCNGC 6 cut(s) 181, 552, 595, 635, 927, 930
Sau3AI GATC 3 cut(s) 310, 334, 982
Sau96I GGNCC 3 cut(s) 10, 532, 684
SchI GAGTC 2 cut(s) 37, 629
ScrFI CCNGG 2 cut(s) 220, 536
SduI GDGCHC 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 1047
SinI GGWCC 2 cut(s) 10, 532
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
SpeI ACTAGT 1 cut(s) 824
Sse9I AATT 9 cut(s) 122, 252, 280, 513, 718, 782, 848, 920, 1007
SsiI CCGC 5 cut(s) 181, 185, 926, 929, 970
SspMI CTAG 3 cut(s) 188, 675, 825
StyD4I CCNGG 2 cut(s) 218, 534
TaaI ACNGT 2 cut(s) 421, 742
TaiI ACGT 1 cut(s) 649
TaqI TCGA 1 cut(s) 1047
TasI AATT 9 cut(s) 122, 252, 280, 513, 718, 782, 848, 920, 1007
TatI WGTACW 2 cut(s) 393, 827
TauI GCSGC 3 cut(s) 183, 929, 932
TfiI GAWTC 1 cut(s) 883
Tru1I TTAA 5 cut(s) 6, 279, 441, 564, 704
Tru9I TTAA 5 cut(s) 6, 279, 441, 564, 704
TscAI CASTG 3 cut(s) 426, 550, 745
TseFI GTSAC 2 cut(s) 40, 814
TseI GCWGC 3 cut(s) 551, 594, 634
Tsp45I GTSAC 2 cut(s) 40, 814
TspDTI ATGAA 8 cut(s) 96, 371, 596, 773, 844, 872, 906, 1050
TspGWI ACGGA 3 cut(s) 75, 89, 303
TspRI CASTG 3 cut(s) 426, 550, 745
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 10, 532
XapI RAATTY 2 cut(s) 718, 1007
XmnI GAANNNNTTC 1 cut(s) 851
XspI CTAG 3 cut(s) 188, 675, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.