Rorug06G0241300

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011175
Physical Location & Seq
Reverse (-)
38457960 .. 38458802
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0241300.1

Sequence Viewer

Length: 843 bp
ATGGCTTACCACACTCGCTCTAACAGCTTCCCCTCCAGGTCACACCCTCTCATTCAAGAAGTTGATGAACAATTGTGCAGATTGACGTCTTTCGAAGCCAGCTCCACATCATCATCATCAATAAGCCACCAACTAAATTGTCTGCAAGACTTGCATGATTGTGTTGATATATTGCTTCAATTGCCACTCACCCAACAAGCCTTAGCCCAAGAGAAGCATGAAAAATGGGCTAATGAACTATTAGATCGCTCTCTCAGGCTCTTGGATATTTGCAGCACTGCCAAAGATGCCTTGTTGCTAACCAAGGAATGCATTCAGAACCTTCAGTCAATCGTCCGAAGAAGAGGAGGAGGAGAATCTGATGTACTCGCAAGTGAAGTTAGGAAATACTTAACCTCCAGGAAGATGGTGAAAAAGTCGCTCCACAAGACTATGGGGAATTTGAAGGAAATGGAAAACAAATCCAATTTCTCTTCCCTCAACAAGGACCAAGAGACAATCGCCATTATTAGCAAGCTGAGAGAAGTTGAAGCAGTCACTCGAACAATGTTTGAACCAATATTGTCCTTCATCTCTGGGCCAAAGGTATCAAGCAGCTGGTCATTTATATCCAAGATGTTGCAGTCAAAAAGAGTAGCTTCACTAGCTTGTGAGGAAGAAACAGAAGTAAACCAATTTGCAGAGGTGGATGCTGCATTGAAGTCACACAAGAGTTTAGACAATGCACAAAACCAGCTTAACAATCTGGAGTCATGCATCCAAGACCAAGAAGAAGGACTCGAGTGCCTATTTAGGCAATTGATAAAAACAAGAGTCTCCCTACTCAACATCCTCAACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

280

Amino Acids

31.69

Weight (kDa)

6.46

Isoelectric Point (pI)

60.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BPS1 PF03087 45 - 277 1.6e-83 Protein BPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 1 cut(s) 484
AgsI TTSAA 6 cut(s) 56, 179, 445, 530, 554, 700
AjnI CCWGG 2 cut(s) 35, 398
AluBI AGCT 7 cut(s) 27, 102, 517, 597, 638, 647, 736
AluI AGCT 7 cut(s) 27, 102, 517, 597, 638, 647, 736
Alw26I GTCTC 2 cut(s) 488, 820
Ama87I CYCGRG 1 cut(s) 779
AoxI GGCC 1 cut(s) 578
ApeKI GCWGC 3 cut(s) 273, 594, 692
ApoI RAATTY 1 cut(s) 439
Asp700I GAANNNNTTC 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 487, 578
AsuHPI GGTGA 2 cut(s) 181, 421
AsuII TTCGAA 1 cut(s) 93
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 1 cut(s) 487
BbvI GCAGC 3 cut(s) 285, 606, 679
BccI CCATC 1 cut(s) 400
BciT130I CCWGG 2 cut(s) 37, 400
BcoDI GTCTC 2 cut(s) 488, 820
BfaI CTAG 2 cut(s) 644, 841
BisI GCNGC 3 cut(s) 274, 595, 693
BlsI GCNGC 3 cut(s) 275, 596, 694
Bme1390I CCNGG 2 cut(s) 37, 400
Bme18I GGWCC 1 cut(s) 487
BmeT110I CYCGRG 1 cut(s) 779
BmgT120I GGNCC 2 cut(s) 487, 578
BmrFI CCNGG 2 cut(s) 37, 400
BmsI GCATC 3 cut(s) 277, 679, 765
BpmI CTGGAG 3 cut(s) 19, 382, 767
Bpu10I CCTNAGC 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 1 cut(s) 484
BseBI CCWGG 2 cut(s) 37, 400
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 3 cut(s) 694, 756, 828
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMII CTCAG 2 cut(s) 268, 509
BseRI GAGGAG 3 cut(s) 360, 363, 366
BseXI GCAGC 3 cut(s) 285, 606, 679
BsgI GTGCAG 1 cut(s) 97
BshFI GGCC 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 779
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 2 cut(s) 488, 820
BsmI GAATGC 2 cut(s) 312, 314
BsnI GGCC 1 cut(s) 580
BsoBI CYCGRG 1 cut(s) 779
Bsp119I TTCGAA 1 cut(s) 93
Bsp143I GATC 1 cut(s) 244
BspANI GGCC 1 cut(s) 580
BspCNI CTCAG 2 cut(s) 267, 510
BspT104I TTCGAA 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 2 cut(s) 37, 400
Bst6I CTCTTC 2 cut(s) 337, 478
BstACI GRCGYC 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 100, 515
BstDEI CTNAG 3 cut(s) 202, 254, 518
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 3 cut(s) 694, 756, 828
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 2 cut(s) 488, 820
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 5 cut(s) 24, 151, 181, 287, 644
BstNI CCWGG 2 cut(s) 37, 400
BstSCI CCNGG 2 cut(s) 35, 398
BstV1I GCAGC 3 cut(s) 285, 606, 679
BstXI CCANNNNNNTGG 1 cut(s) 406
BsuRI GGCC 1 cut(s) 580
BtsCI GGATG 3 cut(s) 694, 756, 828
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 100, 515
Cfr13I GGNCC 2 cut(s) 487, 578
Csp6I GTAC 1 cut(s) 365
CviAII CATG 3 cut(s) 155, 218, 753
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 3 cut(s) 202, 254, 518
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 337, 478
EarI CTCTTC 2 cut(s) 337, 478
Eco130I CCWWGG 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 779
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 35, 398
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 2 cut(s) 314, 758
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 3 cut(s) 158, 221, 756
FaiI YATR 6 cut(s) 156, 170, 219, 434, 608, 754
FalI AAGNNNNNCTT 2 cut(s) 622, 654
FatI CATG 3 cut(s) 154, 217, 752
Fnu4HI GCNGC 3 cut(s) 274, 595, 693
FokI GGATG 3 cut(s) 701, 743, 815
Fsp4HI GCNGC 3 cut(s) 274, 595, 693
FspBI CTAG 2 cut(s) 644, 841
GluI GCNGC 3 cut(s) 274, 595, 693
GsuI CTGGAG 3 cut(s) 19, 382, 767
HaeIII GGCC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 3 cut(s) 158, 221, 756
HinfI GANTC 4 cut(s) 356, 749, 777, 813
HphI GGTGA 2 cut(s) 181, 421
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 3 cut(s) 318, 338, 361
Hpy188III TCNNGA 2 cut(s) 56, 746
Hpy8I GTNNAC 1 cut(s) 670
HpyAV CCTTC 4 cut(s) 332, 439, 577, 767
HpyCH4IV ACGT 1 cut(s) 86
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 151, 181, 287, 644
HpyF3I CTNAG 3 cut(s) 202, 254, 518
HpySE526I ACGT 1 cut(s) 86
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 3 cut(s) 158, 221, 756
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 2 cut(s) 107, 426
Lsp1109I GCAGC 3 cut(s) 285, 606, 679
LweI GCATC 3 cut(s) 277, 679, 765
MaeI CTAG 2 cut(s) 644, 841
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 3 cut(s) 39, 535, 702
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 6 cut(s) 351, 354, 415, 465, 668, 782
MfeI CAATTG 3 cut(s) 71, 179, 797
MluCI AATT 7 cut(s) 71, 136, 179, 439, 466, 674, 797
MlyI GAGTC 3 cut(s) 758, 771, 822
Mph1103I ATGCAT 2 cut(s) 314, 758
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 2 cut(s) 392, 738
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 597
MspR9I CCNGG 2 cut(s) 37, 400
MunI CAATTG 3 cut(s) 71, 179, 797
Mva1269I GAATGC 2 cut(s) 312, 314
MvaI CCWGG 2 cut(s) 37, 400
MwoI GCNNNNNNNGC 5 cut(s) 24, 151, 181, 287, 644
NdeII GATC 1 cut(s) 244
NlaIII CATG 3 cut(s) 158, 221, 756
NmuCI GTSAC 3 cut(s) 39, 535, 702
NsiI ATGCAT 2 cut(s) 314, 758
NspV TTCGAA 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 779
PctI GAATGC 2 cut(s) 312, 314
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 356
PfoI TCCNGGA 1 cut(s) 398
PkrI GCNGC 3 cut(s) 275, 596, 694
PleI GAGTC 3 cut(s) 757, 771, 821
PpsI GAGTC 3 cut(s) 757, 771, 821
Psp6I CCWGG 2 cut(s) 35, 398
PspGI CCWGG 2 cut(s) 35, 398
PspPI GGNCC 2 cut(s) 487, 578
PspXI VCTCGAGB 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 597
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 392, 738
SatI GCNGC 3 cut(s) 274, 595, 693
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 487, 578
SchI GAGTC 3 cut(s) 758, 771, 822
ScrFI CCNGG 2 cut(s) 37, 400
SfaNI GCATC 3 cut(s) 277, 679, 765
Sfr274I CTCGAG 1 cut(s) 779
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 1 cut(s) 487
SlaI CTCGAG 1 cut(s) 779
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 7 cut(s) 71, 136, 179, 439, 466, 674, 797
SspI AATATT 1 cut(s) 561
SspMI CTAG 2 cut(s) 644, 841
StyD4I CCNGG 2 cut(s) 35, 398
StyI CCWWGG 1 cut(s) 303
TaiI ACGT 1 cut(s) 89
TaqI TCGA 3 cut(s) 93, 541, 780
TasI AATT 7 cut(s) 71, 136, 179, 439, 466, 674, 797
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 2 cut(s) 392, 738
Tru9I TTAA 2 cut(s) 392, 738
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 3 cut(s) 39, 535, 702
TseI GCWGC 3 cut(s) 273, 594, 692
Tsp45I GTSAC 3 cut(s) 39, 535, 702
TspDTI ATGAA 4 cut(s) 81, 234, 249, 559
TspRI CASTG 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 487
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 439
XhoI CTCGAG 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 644, 841
ZraI GACGTC 1 cut(s) 87
Zsp2I ATGCAT 2 cut(s) 314, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.