MD11G1309100.v1.1

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011175
Physical Location & Seq
Reverse (-)
42281007 .. 42283426
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1309100.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGACTGTTTAGCGGGCGTTGCACATGCCCCGGCTACTGCTATCTTGCGCCGACCGGTCCTCTACACCACTTCAAGAAGGGATAGAAATGGCGGGTTGAAGTTGCGAGCCAAAATCGTCGAAGAAAACGACCCATTACTTCAATCTGCCATTGATTCGGCTTCTCTTCGTTTCCGGGAGACCCATCGACCAAAGCCTCTGTTTGTTGATCCGTATGCTGGTTGCTTTGTTCCTCCTAATGCTCAGATGGACATGATGCAATGCTCACATCATTATTGCGTTGCTACTAAATTCATCGACGATAAGTTGCTTCGAACGGTAAACCATATCGATGGATTAAAGCAGGTTGTTTTGCTAACCGATGGAATGGATACCCGTCCATATAGGCTTAGTTGGCCAAGTTCAACCATTATATTCAATGTTTCTCCGGAACGGGTGTTCAAAAGAGCAGCTGAGAAGCTTCAAGGTGTTGGGGCTAAGATTCCTAGAAGCTGCTTATCCCTTCACGTTCCTTTGGAATCCTCCGACATACAGAAAAGTCTGCGTGCTAAAGGGTTTAACGGCAATCAACCTAGCATTTGGGCCATGCAGGGGTTGCCTTTGATGACATTAGCAAGTTTTGAAGATATTTTGCTCACTGTAAGTGGTTTGGCAATGAAGGGATGTTTTTTCTTGGGAGAATTACCTGCTTGGTTGGCAGAAACTGAAATAGGGACCAAGACTAGTGCAAGGACATGGTTGGAGAAACTATTCATGAACAATGGCTTTCGGGTGGACATGATTTGTTTTGATGAAGTTGCAAGGAGTTTAGGCAAGGAATTAGCACCGGGAAGGTATAAGAATATACTATTTTCTGCAGACCAACTGCGGTTTTCTGATGATCAGATGAAACTTGGGAGGAAGGAATTCCTGAGAGCCGAGGAAGAAGGGGACGAGGAAGGTTTCGAAGAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

319

Amino Acids

35.73

Weight (kDa)

7.6

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 51 - 199 2.6e-23 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 334, 694
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 3 cut(s) 14, 93, 868
AclWI GGATC 1 cut(s) 203
AcoI YGGCCR 1 cut(s) 395
AcsI RAATTY 2 cut(s) 290, 905
AfiI CCNNNNNNNGG 2 cut(s) 218, 591
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 8 cut(s) 75, 100, 143, 405, 418, 442, 464, 623
AhlI ACTAGT 1 cut(s) 722
AloI GAACNNNNNNTCC 2 cut(s) 422, 454
AluBI AGCT 4 cut(s) 452, 460, 492, 952
AluI AGCT 4 cut(s) 452, 460, 492, 952
Alw21I GWGCWC 1 cut(s) 954
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 1 cut(s) 203
AlwNI CAGNNNCTG 1 cut(s) 704
Aor13HI TCCGGA 1 cut(s) 427
AoxI GGCC 2 cut(s) 395, 582
ApeKI GCWGC 2 cut(s) 449, 492
ApoI RAATTY 2 cut(s) 290, 905
AsiGI ACCGGT 1 cut(s) 55
Asp700I GAANNNNTTC 2 cut(s) 749, 905
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 3 cut(s) 58, 582, 714
AsuC2I CCSGG 3 cut(s) 32, 176, 828
AsuII TTCGAA 2 cut(s) 313, 945
AvaII GGWCC 2 cut(s) 58, 714
BalI TGGCCA 1 cut(s) 397
BanII GRGCYC 1 cut(s) 954
Bbv12I GWGCWC 1 cut(s) 954
BbvI GCAGC 2 cut(s) 461, 479
BccI CCATC 4 cut(s) 192, 241, 326, 356
BceAI ACGGC 1 cut(s) 577
BciVI GTATCC 1 cut(s) 364
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 3 cut(s) 32, 176, 828
BcoDI GTCTC 1 cut(s) 173
BcuI ACTAGT 1 cut(s) 722
BfaI CTAG 3 cut(s) 486, 573, 723
BfmI CTRYAG 1 cut(s) 855
BfuAI ACCTGC 2 cut(s) 334, 694
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 2 cut(s) 450, 493
BlsI GCNGC 2 cut(s) 451, 494
Bme1390I CCNGG 3 cut(s) 32, 176, 828
Bme18I GGWCC 2 cut(s) 58, 714
BmgT120I GGNCC 3 cut(s) 58, 582, 714
BmiI GGNNCC 1 cut(s) 715
BmrFI CCNGG 3 cut(s) 32, 176, 828
BmsI GCATC 1 cut(s) 246
Bpu14I TTCGAA 2 cut(s) 313, 945
BpuMI CCSGG 3 cut(s) 32, 176, 828
Bsa29I ATCGAT 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 30, 918
BsaWI WCCGGW 2 cut(s) 55, 427
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 591
Bse118I RCCGGY 1 cut(s) 55
Bse3DI GCAATG 2 cut(s) 266, 660
BseAI TCCGGA 1 cut(s) 427
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 30, 918
BseGI GGATG 1 cut(s) 668
BseLI CCNNNNNNNGG 2 cut(s) 218, 591
BseMI GCAATG 2 cut(s) 266, 660
BseMII CTCAG 3 cut(s) 257, 444, 902
BseXI GCAGC 2 cut(s) 461, 479
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 2 cut(s) 397, 584
BshTI ACCGGT 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 954
BsiSI CCGG 5 cut(s) 32, 56, 175, 428, 827
BslFI GGGAC 2 cut(s) 727, 944
BslI CCNNNNNNNGG 2 cut(s) 218, 591
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 727, 944
BsnI GGCC 2 cut(s) 397, 584
Bso31I GGTCTC 1 cut(s) 173
Bsp119I TTCGAA 2 cut(s) 313, 945
Bsp1286I GDGCHC 1 cut(s) 954
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 2 cut(s) 208, 880
BspACI CCGC 3 cut(s) 14, 93, 868
BspANI GGCC 2 cut(s) 397, 584
BspCNI CTCAG 3 cut(s) 256, 445, 903
BspDI ATCGAT 1 cut(s) 330
BspEI TCCGGA 1 cut(s) 427
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 1 cut(s) 715
BspMAI CTGCAG 1 cut(s) 859
BspMI ACCTGC 2 cut(s) 334, 694
BspPI GGATC 1 cut(s) 203
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 2 cut(s) 313, 945
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 266, 660
BsrFI RCCGGY 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 30, 918
BssMI GATC 2 cut(s) 208, 880
Bst4CI ACNGT 3 cut(s) 8, 319, 640
Bst6I CTCTTC 2 cut(s) 171, 942
BstAPI GCANNNNNTGC 1 cut(s) 595
BstBI TTCGAA 2 cut(s) 313, 945
BstC8I GCNNGC 3 cut(s) 16, 108, 546
BstDEI CTNAG 5 cut(s) 243, 389, 453, 477, 911
BstF5I GGATG 1 cut(s) 668
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 2 cut(s) 211, 883
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 2 cut(s) 208, 880
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 4 cut(s) 20, 394, 595, 695
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 3 cut(s) 30, 174, 826
BstSFI CTRYAG 1 cut(s) 855
BstV1I GCAGC 2 cut(s) 461, 479
BstXI CCANNNNNNTGG 1 cut(s) 332
Bsu15I ATCGAT 1 cut(s) 330
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 397, 584
BsuTUI ATCGAT 1 cut(s) 330
BtsCI GGATG 1 cut(s) 668
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 2 cut(s) 334, 694
Cac8I GCNNGC 3 cut(s) 16, 108, 546
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 753
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 58, 582, 714
ClaI ATCGAT 1 cut(s) 330
CspAI ACCGGT 1 cut(s) 55
CviAII CATG 6 cut(s) 26, 253, 586, 735, 754, 778
DdeI CTNAG 5 cut(s) 243, 389, 453, 477, 911
DpnI GATC 2 cut(s) 210, 882
DpnII GATC 2 cut(s) 208, 880
EaeI YGGCCR 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 171, 942
EarI CTCTTC 2 cut(s) 171, 942
Ecl136II GAGCTC 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 954
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 2 cut(s) 58, 714
Eco53kI GAGCTC 1 cut(s) 952
EcoICRI GAGCTC 1 cut(s) 952
EcoRI GAATTC 1 cut(s) 905
EcoT38I GRGCYC 1 cut(s) 954
FaeI CATG 6 cut(s) 29, 256, 589, 738, 757, 781
FaqI GGGAC 2 cut(s) 727, 944
FatI CATG 6 cut(s) 25, 252, 585, 734, 753, 777
FauI CCCGC 2 cut(s) 7, 86
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 450, 493
FokI GGATG 1 cut(s) 675
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 2 cut(s) 450, 493
FspBI CTAG 3 cut(s) 486, 573, 723
GlaI GCGC 1 cut(s) 50
GluI GCNGC 2 cut(s) 450, 493
HaeIII GGCC 2 cut(s) 397, 584
HapII CCGG 5 cut(s) 32, 56, 175, 428, 827
HhaI GCGC 1 cut(s) 51
Hin1II CATG 6 cut(s) 29, 256, 589, 738, 757, 781
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 155, 481, 518
HpaII CCGG 5 cut(s) 32, 56, 175, 428, 827
Hpy166II GTNNAC 2 cut(s) 322, 775
Hpy188I TCNGA 4 cut(s) 246, 526, 877, 885
Hpy188III TCNNGA 4 cut(s) 75, 428, 754, 910
Hpy8I GTNNAC 2 cut(s) 322, 775
Hpy99I CGWCG 2 cut(s) 122, 302
HpyAV CCTTC 7 cut(s) 72, 512, 652, 825, 895, 920, 932
HpyCH4III ACNGT 3 cut(s) 8, 319, 640
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 6 cut(s) 23, 259, 589, 728, 800, 857
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 394, 595, 695
HpyF3I CTNAG 5 cut(s) 243, 389, 453, 477, 911
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 6 cut(s) 29, 256, 589, 738, 757, 781
HspAI GCGC 1 cut(s) 49
Kpn2I TCCGGA 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 2 cut(s) 208, 880
LguI GCTCTTC 1 cut(s) 942
Lsp1109I GCAGC 2 cut(s) 461, 479
LweI GCATC 1 cut(s) 246
MaeI CTAG 3 cut(s) 486, 573, 723
MaeII ACGT 1 cut(s) 507
MalI GATC 2 cut(s) 210, 882
MboI GATC 2 cut(s) 208, 880
MboII GAAGA 4 cut(s) 134, 158, 635, 935
MhlI GDGCHC 1 cut(s) 954
MlsI TGGCCA 1 cut(s) 397
MluCI AATT 4 cut(s) 290, 680, 818, 905
MluNI TGGCCA 1 cut(s) 397
MmeI TCCRAC 2 cut(s) 549, 720
MnlI CCTC 7 cut(s) 71, 207, 243, 532, 891, 913, 928
Mox20I TGGCCA 1 cut(s) 397
MroI TCCGGA 1 cut(s) 427
MroXI GAANNNNTTC 2 cut(s) 749, 905
MscI TGGCCA 1 cut(s) 397
MseI TTAA 2 cut(s) 338, 558
MslI CAYNNNNRTG 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 397
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 5 cut(s) 32, 56, 175, 428, 827
MspR9I CCNGG 3 cut(s) 32, 176, 828
MwoI GCNNNNNNNGC 4 cut(s) 20, 394, 595, 695
NciI CCSGG 3 cut(s) 32, 176, 828
NdeII GATC 2 cut(s) 208, 880
NlaIII CATG 6 cut(s) 29, 256, 589, 738, 757, 781
NlaIV GGNNCC 1 cut(s) 715
NmeAIII GCCGAG 1 cut(s) 943
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 2 cut(s) 313, 945
PagI TCATGA 1 cut(s) 753
PciSI GCTCTTC 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 2 cut(s) 749, 905
PfeI GAWTC 3 cut(s) 155, 481, 518
PfoI TCCNGGA 1 cut(s) 174
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 2 cut(s) 451, 494
Psp124BI GAGCTC 1 cut(s) 954
PspN4I GGNNCC 1 cut(s) 715
PspPI GGNCC 3 cut(s) 58, 582, 714
PstI CTGCAG 1 cut(s) 859
PstNI CAGNNNCTG 1 cut(s) 704
PvuII CAGCTG 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 330
SacI GAGCTC 1 cut(s) 954
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 2 cut(s) 338, 558
SatI GCNGC 2 cut(s) 450, 493
Sau3AI GATC 2 cut(s) 208, 880
Sau96I GGNCC 3 cut(s) 58, 582, 714
ScrFI CCNGG 3 cut(s) 32, 176, 828
SduI GDGCHC 1 cut(s) 954
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 855
SfuI TTCGAA 2 cut(s) 313, 945
SinI GGWCC 2 cut(s) 58, 714
SmiMI CAYNNNNRTG 1 cut(s) 330
SpeI ACTAGT 1 cut(s) 722
Sse9I AATT 4 cut(s) 290, 680, 818, 905
SsiI CCGC 3 cut(s) 14, 93, 868
SspMI CTAG 3 cut(s) 486, 573, 723
SstI GAGCTC 1 cut(s) 954
StyD4I CCNGG 3 cut(s) 30, 174, 826
TaaI ACNGT 3 cut(s) 8, 319, 640
TaiI ACGT 1 cut(s) 510
TaqI TCGA 6 cut(s) 120, 187, 297, 313, 330, 945
TasI AATT 4 cut(s) 290, 680, 818, 905
TfiI GAWTC 3 cut(s) 155, 481, 518
Tru1I TTAA 2 cut(s) 338, 558
Tru9I TTAA 2 cut(s) 338, 558
TscAI CASTG 1 cut(s) 643
TseI GCWGC 2 cut(s) 449, 492
TspDTI ATGAA 6 cut(s) 283, 671, 742, 770, 807, 902
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 58, 714
XapI RAATTY 2 cut(s) 290, 905
XceI RCATGY 1 cut(s) 29
XmnI GAANNNNTTC 2 cut(s) 749, 905
XspI CTAG 3 cut(s) 486, 573, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.