Rh6AG353900

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011175
Physical Location & Seq
Forward (+)
55406418 .. 55408729
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG353900.1

Sequence Viewer

Length: 741 bp
ATGGACTCTTTACTGGGCTTCCCACAATCCCCAGCTACTGCCATCTTGCGCCGCCCGCTAATCTCCACTTCAAGACCAAACAGTAACAGAAATGCCGGGTTGAGTTTCAGAGCAAAACCCAACGACGCAATTGACCCATTACTCCACGACGCCGTTAATTCAGCTTCTCTTCGTTTCCGAGAGACCCATCGACCAGAGCCTCTCTTTGTTGATCCATATGCCGGTTGCTTTGTTGATCCTAATGCGCAGATGGACATGAAGCAATGCTCACACCATTATTGCATTGCAACTAAGTACATTGATGATAAGTTACTTCGTACAGTGAACCATATTGAGGGACTTAAGCAGGTTGTTTTGCTAACAGATGGCATGGACACCCGGCCGTATAGGCTTAGTTGGCCAACTTCAACCATAATTTTTGATATTTCGCCGGACCGGGTATTCAGTGGAGCAGCTGAAAAGCTTAAAGGTGTTGGGGCTAAAATCCCAAGAAGCTGCTTATTCCTTCATGTTCCATTGGAGTCCTCCGATTTGCAGCAAACTCTACGTGCTAAAGGCTTCAATGGTAACCGGCCTAGTATATGGGCCATGCAGGGACTGCCCGTAATGACATTGGCAAATTTTGAGGACATTTTGCTCACTGTTAGTGGTTTGTCCATGAATGGATGTGGCTTTTTGGGAGAATTGCCTGCTTGGTTGGCAGAGACTGAAATAGTGACCAAGGTTGGTTGGCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
LCM

Protein Analysis

246

Amino Acids

27.23

Weight (kDa)

7.68

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 51 - 200 4.3e-24 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02405 AT4G02405
fragaria_vesca FvH4_2g25800 FvH4_2g25800
malus_domestica MD03G1288800.v1.1 MD11G1309100.v1.1
prunus_persica Prupe.8G262100_v2.0.a1
pyrus_communis pycom03g22890 pycom11g27040
rosa_chinensis RchiOBHm_Chr6g0294111
rosa_laevigata RLG00000011898
rosa_multiflora Rmu_sc0005037.1_g000010
rosa_roxburghii Rroxscaffold_7G00173640
rosa_rugosa Rorug06G0241200 Rorug06G0241300
rosa_samantha Rh6AG353900 Rh6BG360800 Rh6CG368000 Rh6DG354700
rosa_wichuraiana Rw6G030840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 337
AciI CCGC 2 cut(s) 52, 56
AclWI GGATC 2 cut(s) 206, 230
AcoI YGGCCR 2 cut(s) 380, 398
AcsI RAATTY 1 cut(s) 619
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 2 cut(s) 296, 319
AfiI CCNNNNNNNGG 2 cut(s) 221, 334
AflII CTTAAG 1 cut(s) 341
AgsI TTSAA 3 cut(s) 72, 408, 562
AluBI AGCT 5 cut(s) 35, 164, 455, 463, 495
AluI AGCT 5 cut(s) 35, 164, 455, 463, 495
Alw26I GTCTC 2 cut(s) 176, 698
AlwI GGATC 2 cut(s) 206, 230
AlwNI CAGNNNCTG 3 cut(s) 38, 598, 707
AoxI GGCC 4 cut(s) 380, 398, 572, 585
ApeKI GCWGC 3 cut(s) 452, 495, 535
ApoI RAATTY 1 cut(s) 619
AspLEI GCGC 2 cut(s) 51, 247
AspS9I GGNCC 2 cut(s) 433, 585
AsuC2I CCSGG 3 cut(s) 97, 379, 437
AvaII GGWCC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 400
BbvI GCAGC 3 cut(s) 464, 482, 547
BccI CCATC 4 cut(s) 50, 195, 244, 359
BceAI ACGGC 2 cut(s) 137, 367
BcnI CCSGG 3 cut(s) 97, 379, 437
BcoDI GTCTC 2 cut(s) 176, 698
BfaI CTAG 1 cut(s) 576
BfrI CTTAAG 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 388
BisI GCNGC 4 cut(s) 52, 453, 496, 536
BlsI GCNGC 4 cut(s) 53, 454, 497, 537
Bme1390I CCNGG 3 cut(s) 97, 379, 437
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 433, 585
BmrFI CCNGG 3 cut(s) 97, 379, 437
BmrI ACTGGG 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 23
BpuMI CCSGG 3 cut(s) 97, 379, 437
BsaAI YACGTR 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 720
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 334
Bse118I RCCGGY 2 cut(s) 221, 570
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 2 cut(s) 269, 282
BseDI CCNNGG 1 cut(s) 720
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 2 cut(s) 221, 334
BseMI GCAATG 2 cut(s) 269, 282
BseNI ACTGG 1 cut(s) 18
BseX3I CGGCCG 1 cut(s) 380
BseXI GCAGC 3 cut(s) 464, 482, 547
BseYI CCCAGC 1 cut(s) 31
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 4 cut(s) 382, 400, 574, 587
BsiEI CGRYCG 1 cut(s) 383
BsiSI CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
BslFI GGGAC 2 cut(s) 351, 609
BslI CCNNNNNNNGG 2 cut(s) 221, 334
BsmAI GTCTC 2 cut(s) 176, 698
BsmFI GGGAC 2 cut(s) 351, 609
BsnI GGCC 4 cut(s) 382, 400, 574, 587
Bso31I GGTCTC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 211, 235
BspACI CCGC 2 cut(s) 52, 56
BspANI GGCC 4 cut(s) 382, 400, 574, 587
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 206, 230
BspTI CTTAAG 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 269, 282
BsrFI RCCGGY 2 cut(s) 221, 570
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 2 cut(s) 221, 570
BssECI CCNNGG 1 cut(s) 720
BssMI GATC 2 cut(s) 211, 235
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 720
Bst4CI ACNGT 3 cut(s) 83, 322, 643
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 598
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 56, 690
BstDEI CTNAG 2 cut(s) 291, 392
BstEII GGTNACC 1 cut(s) 566
BstF5I GGATG 1 cut(s) 671
BstHHI GCGC 2 cut(s) 51, 247
BstKTI GATC 2 cut(s) 214, 238
BstMAI GTCTC 2 cut(s) 176, 698
BstMBI GATC 2 cut(s) 211, 235
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 5 cut(s) 55, 388, 397, 598, 698
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 3 cut(s) 95, 377, 435
BstV1I GCAGC 3 cut(s) 464, 482, 547
BstZI CGGCCG 1 cut(s) 380
BsuRI GGCC 4 cut(s) 382, 400, 574, 587
BtsCI GGATG 1 cut(s) 671
BtsIMutI CAGTG 3 cut(s) 327, 451, 639
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 2 cut(s) 56, 690
CaiI CAGNNNCTG 3 cut(s) 38, 598, 707
CfoI GCGC 2 cut(s) 51, 247
Cfr10I RCCGGY 2 cut(s) 221, 570
Cfr13I GGNCC 2 cut(s) 433, 585
CpoI CGGWCCG 1 cut(s) 433
CseI GACGC 2 cut(s) 134, 158
Csp6I GTAC 2 cut(s) 295, 318
CspI CGGWCCG 1 cut(s) 433
CviAII CATG 5 cut(s) 256, 370, 509, 589, 658
CviQI GTAC 2 cut(s) 295, 318
DdeI CTNAG 2 cut(s) 291, 392
DpnI GATC 2 cut(s) 213, 237
DpnII GATC 2 cut(s) 211, 235
EaeI YGGCCR 2 cut(s) 380, 398
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 433
Eco52I CGGCCG 1 cut(s) 380
Eco91I GGTNACC 1 cut(s) 566
EcoO65I GGTNACC 1 cut(s) 566
EcoT14I CCWWGG 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 5 cut(s) 259, 373, 512, 592, 661
FaqI GGGAC 2 cut(s) 351, 609
FatI CATG 5 cut(s) 255, 369, 508, 588, 657
FauI CCCGC 1 cut(s) 63
FauNDI CATATG 1 cut(s) 217
Fnu4HI GCNGC 4 cut(s) 52, 453, 496, 536
FokI GGATG 1 cut(s) 678
Fsp4HI GCNGC 4 cut(s) 52, 453, 496, 536
FspBI CTAG 1 cut(s) 576
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 2 cut(s) 50, 246
GluI GCNGC 4 cut(s) 52, 453, 496, 536
GsaI CCCAGC 1 cut(s) 35
HaeIII GGCC 4 cut(s) 382, 400, 574, 587
HapII CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
HgaI GACGC 2 cut(s) 134, 158
HhaI GCGC 2 cut(s) 51, 247
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 5 cut(s) 259, 373, 512, 592, 661
Hin6I GCGC 2 cut(s) 49, 245
HinP1I GCGC 2 cut(s) 49, 245
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 2 cut(s) 5, 521
HpaII CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 3 cut(s) 110, 179, 529
Hpy188III TCNNGA 1 cut(s) 72
Hpy8I GTNNAC 1 cut(s) 325
Hpy99I CGWCG 2 cut(s) 128, 152
HpyAV CCTTC 1 cut(s) 515
HpyCH4III ACNGT 3 cut(s) 83, 322, 643
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 4 cut(s) 282, 287, 535, 592
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 388, 397, 598, 698
HpyF3I CTNAG 2 cut(s) 291, 392
HpySE526I ACGT 1 cut(s) 547
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 5 cut(s) 259, 373, 512, 592, 661
HspAI GCGC 2 cut(s) 49, 245
Kzo9I GATC 2 cut(s) 211, 235
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 3 cut(s) 464, 482, 547
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 4 cut(s) 83, 309, 566, 715
MalI GATC 2 cut(s) 213, 237
MboI GATC 2 cut(s) 211, 235
MboII GAAGA 1 cut(s) 161
MfeI CAATTG 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 5 cut(s) 129, 157, 414, 619, 683
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 1 cut(s) 530
MnlI CCTC 4 cut(s) 210, 328, 535, 619
Mox20I TGGCCA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 400
MseI TTAA 4 cut(s) 156, 342, 465, 739
Msp20I TGGCCA 1 cut(s) 400
MspA1I CMGCKG 1 cut(s) 455
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 6 cut(s) 96, 222, 379, 431, 436, 571
MspR9I CCNGG 3 cut(s) 97, 379, 437
MunI CAATTG 1 cut(s) 129
MwoI GCNNNNNNNGC 5 cut(s) 55, 388, 397, 598, 698
NciI CCSGG 3 cut(s) 97, 379, 437
NdeI CATATG 1 cut(s) 217
NdeII GATC 2 cut(s) 211, 235
NlaIII CATG 5 cut(s) 259, 373, 512, 592, 661
NmuCI GTSAC 1 cut(s) 715
NsbI TGCGCA 1 cut(s) 246
PkrI GCNGC 4 cut(s) 53, 454, 497, 537
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 548
PspEI GGTNACC 1 cut(s) 566
PspFI CCCAGC 1 cut(s) 31
PspPI GGNCC 2 cut(s) 433, 585
PstNI CAGNNNCTG 3 cut(s) 38, 598, 707
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 2 cut(s) 296, 319
RsaNI GTAC 2 cut(s) 295, 318
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SaqAI TTAA 4 cut(s) 156, 342, 465, 739
SatI GCNGC 4 cut(s) 52, 453, 496, 536
Sau3AI GATC 2 cut(s) 211, 235
Sau96I GGNCC 2 cut(s) 433, 585
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 3 cut(s) 97, 379, 437
SetI ASST 9 cut(s) 37, 166, 351, 457, 465, 472, 497, 550, 726
SinI GGWCC 1 cut(s) 433
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 5 cut(s) 129, 157, 414, 619, 683
SsiI CCGC 2 cut(s) 52, 56
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 95, 377, 435
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 3 cut(s) 83, 322, 643
TaiI ACGT 1 cut(s) 550
TaqI TCGA 1 cut(s) 190
TasI AATT 5 cut(s) 129, 157, 414, 619, 683
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 4 cut(s) 156, 342, 465, 739
Tru9I TTAA 4 cut(s) 156, 342, 465, 739
TscAI CASTG 3 cut(s) 327, 451, 646
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 3 cut(s) 452, 495, 535
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 3 cut(s) 272, 497, 674
TspRI CASTG 3 cut(s) 327, 451, 646
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 1 cut(s) 619
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.