AT4G02425

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009253
Physical Location & Seq
Reverse (-)
1067052 .. 1068821
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02425.1

Sequence Viewer

Length: 789 bp
ATGTCTCCGAAACATCTAGAGTCATCACGAAGCTCTATTGAATCATGCACTTCACAGCTTCTCTCATGGCGACCATTTCACCGCTCCAAAACCCTAGACTCATCTGACCAACCACCGCAGACCAATGGGTTTCACTCCTTTACTCCCAAACGCCCTTGCTTCTCCGATCGATCCACTTCTTTCACCATCGAAGCTATGAGCCGTCTCTCACTCGCCGACGACGACAATGGAGGGAAGACATTATCAGCTTCCAATTACAGCAATAGAGGAAGTTTCAGGTTAGTAGCGAGGAAGCGGCGGCGGCGTAATTCGAGATCGGTGTCTGGTCGGAGTAGTGATCGGAGTGGGACTCGGAGATGTTGCTCCATTGGTGCTCATGGGACTTGTTCGGATTTGCCTTTCGCTGTTGGTACAGATTCAAGTGGAGAGCTTTTTGGTGAAGCGAATTGGGCTTCTGATGTGAGTGAGGCGGCGAGGAATTCACGGAGAGAGCGGCGAGATTCTGGTGGAGAGAAGGAAGCTTCTGGTGGATTTGGATTTGCTAATGGAGTTGATCCAATGGGGAATGAATCTGGGTATGGGAGTGAGCCTGGTTACAGAGGTGATGCTGAGTTTGGCTATGGTGATGAATTTGATGATGAAGAAGAAGATGTCAAGCCATTGTTTTGGGGAGATACAGATTCAACAATGGGGATGTCTGGTGAGACGAAATTCTCAGATAGTAAACCTCAATTCAGGTGCCGGCGAAGAAGACAACATGACTACAAAACTGTTGACTCCATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.02

Weight (kDa)

7.63

Isoelectric Point (pI)

69.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 738
AccBSI CCGCTC 2 cut(s) 84, 493
AciI CCGC 7 cut(s) 82, 116, 295, 298, 301, 470, 493
AclWI GGATC 2 cut(s) 165, 548
AcsI RAATTY 3 cut(s) 478, 629, 710
AfaI GTAC 1 cut(s) 412
AgsI TTSAA 3 cut(s) 41, 420, 684
AjnI CCWGG 1 cut(s) 589
AluBI AGCT 6 cut(s) 33, 58, 194, 248, 430, 521
AluI AGCT 6 cut(s) 33, 58, 194, 248, 430, 521
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 3 cut(s) 9, 209, 698
AlwI GGATC 2 cut(s) 165, 548
ApoI RAATTY 3 cut(s) 478, 629, 710
AsuHPI GGTGA 6 cut(s) 71, 175, 449, 614, 635, 713
BanI GGYRCC 1 cut(s) 738
BbsI GAAGAC 2 cut(s) 242, 757
Bbv12I GWGCWC 1 cut(s) 376
BccI CCATC 1 cut(s) 194
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 591
BcoDI GTCTC 3 cut(s) 9, 209, 698
BfaI CTAG 2 cut(s) 17, 95
BisI GCNGC 5 cut(s) 296, 299, 302, 471, 494
BlsI GCNGC 5 cut(s) 297, 300, 303, 472, 495
Bme1390I CCNGG 1 cut(s) 591
BmiI GGNNCC 1 cut(s) 740
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 1 cut(s) 595
BpiI GAAGAC 2 cut(s) 242, 757
BplI GAGNNNNNCTC 2 cut(s) 334, 366
Bsa29I ATCGAT 1 cut(s) 169
Bse118I RCCGGY 1 cut(s) 741
BseBI CCWGG 1 cut(s) 591
BseCI ATCGAT 1 cut(s) 169
BseGI GGATG 1 cut(s) 699
BseMII CTCAG 2 cut(s) 600, 729
Bsh1285I CGRYCG 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 738
BshVI ATCGAT 1 cut(s) 169
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 376
BsiSI CCGG 1 cut(s) 742
BslFI GGGAC 2 cut(s) 361, 394
BsmAI GTCTC 3 cut(s) 9, 209, 698
BsmBI CGTCTC 2 cut(s) 209, 698
BsmFI GGGAC 2 cut(s) 361, 394
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 5 cut(s) 166, 170, 314, 337, 553
BspACI CCGC 7 cut(s) 82, 116, 295, 298, 301, 470, 493
BspCNI CTCAG 2 cut(s) 601, 728
BspDI ATCGAT 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 740
BspPI GGATC 2 cut(s) 165, 548
BspT107I GGYRCC 1 cut(s) 738
BsrBI CCGCTC 2 cut(s) 84, 493
BsrFI RCCGGY 1 cut(s) 741
BssAI RCCGGY 1 cut(s) 741
BssMI GATC 5 cut(s) 166, 170, 314, 337, 553
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 1 cut(s) 772
BstC8I GCNNGC 1 cut(s) 743
BstDEI CTNAG 2 cut(s) 609, 715
BstF5I GGATG 1 cut(s) 699
BstKTI GATC 5 cut(s) 169, 173, 317, 340, 556
BstMAI GTCTC 3 cut(s) 9, 209, 698
BstMBI GATC 5 cut(s) 166, 170, 314, 337, 553
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 301, 449
BstNI CCWGG 1 cut(s) 591
BstSCI CCNGG 1 cut(s) 589
BstV2I GAAGAC 2 cut(s) 242, 757
BstXI CCANNNNNNTGG 1 cut(s) 666
Bsu15I ATCGAT 1 cut(s) 169
BsuTUI ATCGAT 1 cut(s) 169
BtsCI GGATG 1 cut(s) 699
Cac8I GCNNGC 1 cut(s) 743
Cfr10I RCCGGY 1 cut(s) 741
ClaI ATCGAT 1 cut(s) 169
Csp6I GTAC 1 cut(s) 411
CviAII CATG 5 cut(s) 45, 66, 377, 758, 781
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 2 cut(s) 609, 715
DpnI GATC 5 cut(s) 168, 172, 316, 339, 555
DpnII GATC 5 cut(s) 166, 170, 314, 337, 553
EcoRI GAATTC 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 589
Esp3I CGTCTC 2 cut(s) 209, 698
FaeI CATG 5 cut(s) 48, 69, 380, 761, 784
FaiI YATR 8 cut(s) 46, 67, 197, 378, 579, 621, 759, 782
FaqI GGGAC 2 cut(s) 361, 394
FatI CATG 5 cut(s) 44, 65, 376, 757, 780
Fnu4HI GCNGC 5 cut(s) 296, 299, 302, 471, 494
FokI GGATG 1 cut(s) 706
Fsp4HI GCNGC 5 cut(s) 296, 299, 302, 471, 494
FspBI CTAG 2 cut(s) 17, 95
GluI GCNGC 5 cut(s) 296, 299, 302, 471, 494
HapII CCGG 1 cut(s) 742
Hin1II CATG 5 cut(s) 48, 69, 380, 761, 784
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 9 cut(s) 20, 41, 98, 349, 416, 500, 569, 680, 776
HpaII CCGG 1 cut(s) 742
HphI GGTGA 6 cut(s) 71, 175, 449, 614, 635, 713
Hpy166II GTNNAC 2 cut(s) 725, 775
Hpy188I TCNGA 9 cut(s) 9, 106, 166, 330, 342, 354, 391, 457, 718
Hpy188III TCNNGA 3 cut(s) 17, 27, 312
Hpy8I GTNNAC 2 cut(s) 725, 775
Hpy99I CGWCG 2 cut(s) 221, 224
HpyAV CCTTC 1 cut(s) 508
HpyCH4III ACNGT 1 cut(s) 772
HpyCH4V TGCA 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 449
HpyF3I CTNAG 2 cut(s) 609, 715
Hsp92II CATG 5 cut(s) 48, 69, 380, 761, 784
KroI GCCGGC 1 cut(s) 741
KroNI GCCGGC 1 cut(s) 743
Kzo9I GATC 5 cut(s) 166, 170, 314, 337, 553
LmnI GCTCC 2 cut(s) 89, 368
LweI GCATC 1 cut(s) 595
MaeI CTAG 2 cut(s) 17, 95
MaeIII GTNAC 1 cut(s) 593
MalI GATC 5 cut(s) 168, 172, 316, 339, 555
MbiI CCGCTC 2 cut(s) 84, 493
MboI GATC 5 cut(s) 166, 170, 314, 337, 553
MboII GAAGA 6 cut(s) 247, 653, 656, 659, 759, 762
MhlI GDGCHC 1 cut(s) 376
MluCI AATT 7 cut(s) 253, 307, 445, 478, 629, 710, 731
MlyI GAGTC 4 cut(s) 29, 92, 343, 770
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 7 cut(s) 224, 260, 282, 460, 468, 593, 738
MroNI GCCGGC 1 cut(s) 741
MspI CCGG 1 cut(s) 742
MspR9I CCNGG 1 cut(s) 591
MvaI CCWGG 1 cut(s) 591
MwoI GCNNNNNNNGC 2 cut(s) 301, 449
NaeI GCCGGC 1 cut(s) 743
NdeII GATC 5 cut(s) 166, 170, 314, 337, 553
NgoMIV GCCGGC 1 cut(s) 741
NlaIII CATG 5 cut(s) 48, 69, 380, 761, 784
NlaIV GGNNCC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 743
PfeI GAWTC 5 cut(s) 41, 416, 500, 569, 680
PkrI GCNGC 5 cut(s) 297, 300, 303, 472, 495
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 4 cut(s) 28, 92, 343, 770
PpsI GAGTC 4 cut(s) 28, 92, 343, 770
Psp6I CCWGG 1 cut(s) 589
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 1 cut(s) 740
PvuI CGATCG 1 cut(s) 169
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SatI GCNGC 5 cut(s) 296, 299, 302, 471, 494
Sau3AI GATC 5 cut(s) 166, 170, 314, 337, 553
SchI GAGTC 4 cut(s) 29, 92, 343, 770
ScrFI CCNGG 1 cut(s) 591
SduI GDGCHC 1 cut(s) 376
SfaNI GCATC 1 cut(s) 595
Sse9I AATT 7 cut(s) 253, 307, 445, 478, 629, 710, 731
SsiI CCGC 7 cut(s) 82, 116, 295, 298, 301, 470, 493
SspMI CTAG 2 cut(s) 17, 95
StyD4I CCNGG 1 cut(s) 589
TaaI ACNGT 1 cut(s) 772
TaqI TCGA 3 cut(s) 169, 189, 311
TasI AATT 7 cut(s) 253, 307, 445, 478, 629, 710, 731
TauI GCSGC 5 cut(s) 298, 301, 304, 473, 496
TfiI GAWTC 5 cut(s) 41, 416, 500, 569, 680
TspDTI ATGAA 3 cut(s) 582, 642, 654
TspGWI ACGGA 1 cut(s) 499
XapI RAATTY 3 cut(s) 478, 629, 710
XbaI TCTAGA 1 cut(s) 16
XspI CTAG 2 cut(s) 17, 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.