FvH4_2g25690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009253
Physical Location & Seq
Forward (+)
20759784 .. 20769348
9565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25690.t1

Sequence Viewer

Length: 816 bp
ATGTCCCACAAAGCCCTGGACTCACGGCCCTCCTTAGATTCGTGTACATTTCAACTCCATAGCTGGAGACCTTTTCAGCTTCAACAACAGCCAACCAAAACCCTAGACTCCGATCCCGCCAACCCCAAACCCTACCACACCAAGCGCCCATGCCTCTCCAACCGAGCCACGTCGTCGTTTTCCATCGACGCCATCGACATGTCCCGGCTGACTCTGGTCGACGACGACCGGACCATCTCCGGAGGGCACCACCACAAGCACGGGAGCTTCCGATTCCTTGCCAGGAAGAGGCGGCGGCACGGGTCCAGGTCGGTGTCGGGTCGGAGCAGCGATCGGAGCGGGACCCGGAGGTGCTGCTCCGTCGGGGCTTCCGCTGCTCACGGGACGTGTTCGGATTTTCCGGTGGCGATTGGGACGGACTCGAGTGGGGAGCTGTTTGGGAATGGGGATGCGAATTGGGCATCGGATGTGAGTGAGGCTAGGAATTTGAGGAAGGAGAGGGATGGAGTTGGGAGTGGAGAGAAGGAGACTACTCCGGGTGTCGGGTTTGGGCCGGGTGGGGGGTTTGATGCTCAGGGAAATGAATCCGGGTATGGTAGCGAACCGGGTTACCGTGGGGACGCCGAGTTTGGGTATGGTGATGAACTTGATGAGGAGGAGGAGGATGCTCGTCTGTTGTTTTGGGGCAATCGATTTGGAGATAGCGATACAATGATGGAGGTTGTGGGTGAGAATACTTTCACAGATCAAAAATCGCATCACAGATGTCGCCGTAAGAAACACGATTGCAGAATGGTTGTCGATTCACTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

29.86

Weight (kDa)

6.63

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 246
AccBSI CCGCTC 1 cut(s) 339
AccI GTMKAC 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 5 cut(s) 117, 292, 295, 339, 372
AclWI GGATC 1 cut(s) 107
AcsI RAATTY 1 cut(s) 484
AcyI GRCGYC 2 cut(s) 189, 621
AfaI GTAC 1 cut(s) 46
AfiI CCNNNNNNNGG 4 cut(s) 288, 542, 560, 630
AflIII ACRYGT 2 cut(s) 198, 386
AgsI TTSAA 2 cut(s) 53, 83
AjiI CACGTC 2 cut(s) 171, 387
AjnI CCWGG 3 cut(s) 15, 281, 305
AleI CACNNNNGTG 1 cut(s) 811
AluBI AGCT 4 cut(s) 63, 79, 267, 433
AluI AGCT 4 cut(s) 63, 79, 267, 433
Alw26I GTCTC 2 cut(s) 61, 521
AlwI GGATC 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 421
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 2 cut(s) 26, 551
ApeKI GCWGC 3 cut(s) 327, 354, 374
ApoI RAATTY 1 cut(s) 484
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
Asp700I GAANNNNTTC 1 cut(s) 737
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 5 cut(s) 27, 231, 303, 342, 551
AsuC2I CCSGG 6 cut(s) 205, 346, 537, 555, 589, 606
AsuHPI GGTGA 2 cut(s) 650, 740
AvaI CYCGRG 1 cut(s) 421
AvaII GGWCC 3 cut(s) 231, 303, 342
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 339, 341, 361
BccI CCATC 5 cut(s) 191, 200, 242, 497, 709
BceAI ACGGC 2 cut(s) 41, 756
BciT130I CCWGG 3 cut(s) 17, 283, 307
BcnI CCSGG 6 cut(s) 205, 346, 537, 555, 589, 606
BcoDI GTCTC 2 cut(s) 61, 521
BfaI CTAG 2 cut(s) 104, 480
BfoI RGCGCY 1 cut(s) 148
BisI GCNGC 5 cut(s) 293, 296, 328, 355, 375
BlsI GCNGC 5 cut(s) 294, 297, 329, 356, 376
Bme1390I CCNGG 9 cut(s) 17, 205, 283, 307, 346, 537, 555, 589, 606
Bme18I GGWCC 3 cut(s) 231, 303, 342
BmeT110I CYCGRG 1 cut(s) 421
BmgBI CACGTC 2 cut(s) 171, 387
BmgT120I GGNCC 5 cut(s) 27, 231, 303, 342, 551
BmiI GGNNCC 4 cut(s) 248, 304, 343, 344
BmrFI CCNGG 9 cut(s) 17, 205, 283, 307, 346, 537, 555, 589, 606
BmsI GCATC 5 cut(s) 439, 470, 559, 655, 766
BoxI GACNNNNGTC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 573
BpuMI CCSGG 6 cut(s) 205, 346, 537, 555, 589, 606
Bsa29I ATCGAT 1 cut(s) 691
BsaHI GRCGYC 2 cut(s) 189, 621
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 15, 613
BsaWI WCCGGW 3 cut(s) 228, 239, 400
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 542, 560, 630
BseAI TCCGGA 1 cut(s) 239
BseBI CCWGG 3 cut(s) 17, 283, 307
BseCI ATCGAT 1 cut(s) 691
BseDI CCNNGG 2 cut(s) 15, 613
BseGI GGATG 4 cut(s) 454, 472, 508, 670
BseLI CCNNNNNNNGG 4 cut(s) 288, 542, 560, 630
BseMII CTCAG 2 cut(s) 587, 800
BseRI GAGGAG 3 cut(s) 668, 671, 674
BseSI GKGCMC 1 cut(s) 249
BseXI GCAGC 3 cut(s) 339, 341, 361
Bsh1285I CGRYCG 2 cut(s) 229, 334
BshFI GGCC 2 cut(s) 28, 553
BshNI GGYRCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 691
BsiEI CGRYCG 2 cut(s) 229, 334
BsiHKCI CYCGRG 1 cut(s) 421
BsiSI CCGG 9 cut(s) 205, 229, 240, 346, 401, 536, 554, 588, 605
BslFI GGGAC 5 cut(s) 187, 355, 397, 427, 632
BslI CCNNNNNNNGG 4 cut(s) 288, 542, 560, 630
BsmAI GTCTC 2 cut(s) 61, 521
BsmFI GGGAC 5 cut(s) 187, 355, 397, 427, 632
BsnI GGCC 2 cut(s) 28, 553
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 239
Bsp1407I TGTACA 1 cut(s) 44
Bsp143I GATC 3 cut(s) 112, 331, 745
BspACI CCGC 5 cut(s) 117, 292, 295, 339, 372
BspANI GGCC 2 cut(s) 28, 553
BspCNI CTCAG 2 cut(s) 586, 801
BspDI ATCGAT 1 cut(s) 691
BspEI TCCGGA 1 cut(s) 239
BspLI GGNNCC 4 cut(s) 248, 304, 343, 344
BspPI GGATC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 339
BsrGI TGTACA 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 15, 613
BssMI GATC 3 cut(s) 112, 331, 745
BssNI GRCGYC 2 cut(s) 189, 621
Bst2UI CCWGG 3 cut(s) 17, 283, 307
Bst4CI ACNGT 1 cut(s) 614
Bst6I CTCTTC 1 cut(s) 281
BstACI GRCGYC 2 cut(s) 189, 621
BstAUI TGTACA 1 cut(s) 44
BstDEI CTNAG 3 cut(s) 34, 573, 809
BstDSI CCRYGG 1 cut(s) 613
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 4 cut(s) 454, 472, 508, 670
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 3 cut(s) 115, 334, 748
BstMAI GTCTC 2 cut(s) 61, 521
BstMBI GATC 3 cut(s) 112, 331, 745
BstMCI CGRYCG 2 cut(s) 229, 334
BstMWI GCNNNNNNNGC 3 cut(s) 336, 374, 458
BstNI CCWGG 3 cut(s) 17, 283, 307
BstNSI RCATGY 1 cut(s) 202
BstPAI GACNNNNGTC 1 cut(s) 215
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 9 cut(s) 15, 203, 281, 305, 344, 535, 553, 587, 604
BstSLI GKGCMC 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 339, 341, 361
Bsu15I ATCGAT 1 cut(s) 691
BsuRI GGCC 2 cut(s) 28, 553
BsuTUI ATCGAT 1 cut(s) 691
BtgI CCRYGG 1 cut(s) 613
BtrI CACGTC 2 cut(s) 171, 387
BtsCI GGATG 4 cut(s) 454, 472, 508, 670
BtsIMutI CAGTG 1 cut(s) 806
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 5 cut(s) 27, 231, 303, 342, 551
ClaI ATCGAT 1 cut(s) 691
CseI GACGC 2 cut(s) 197, 629
Csp6I GTAC 1 cut(s) 45
CviAII CATG 2 cut(s) 150, 199
CviQI GTAC 1 cut(s) 45
DdeI CTNAG 3 cut(s) 34, 573, 809
DpnI GATC 3 cut(s) 114, 333, 747
DpnII GATC 3 cut(s) 112, 331, 745
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 3 cut(s) 231, 303, 342
Eco88I CYCGRG 1 cut(s) 421
Eco91I GGTNACC 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 342
EcoO65I GGTNACC 1 cut(s) 608
EcoRII CCWGG 3 cut(s) 15, 281, 305
FaeI CATG 2 cut(s) 153, 202
FaiI YATR 5 cut(s) 60, 151, 200, 594, 636
FaqI GGGAC 5 cut(s) 187, 355, 397, 427, 632
FatI CATG 2 cut(s) 149, 198
FauI CCCGC 2 cut(s) 124, 332
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 293, 296, 328, 355, 375
FokI GGATG 4 cut(s) 461, 479, 515, 677
Fsp4HI GCNGC 5 cut(s) 293, 296, 328, 355, 375
FspBI CTAG 2 cut(s) 104, 480
GlaI GCGC 1 cut(s) 146
GluI GCNGC 5 cut(s) 293, 296, 328, 355, 375
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 2 cut(s) 28, 553
HapII CCGG 9 cut(s) 205, 229, 240, 346, 401, 536, 554, 588, 605
HgaI GACGC 2 cut(s) 197, 629
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 2 cut(s) 189, 621
Hin1II CATG 2 cut(s) 153, 202
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 8 cut(s) 20, 38, 107, 211, 273, 419, 584, 803
HpaII CCGG 9 cut(s) 205, 229, 240, 346, 401, 536, 554, 588, 605
HphI GGTGA 2 cut(s) 650, 740
Hpy166II GTNNAC 2 cut(s) 45, 220
Hpy188I TCNGA 6 cut(s) 112, 272, 324, 336, 394, 466
Hpy188III TCNNGA 1 cut(s) 240
Hpy8I GTNNAC 2 cut(s) 45, 220
Hpy99I CGWCG 6 cut(s) 175, 178, 191, 224, 227, 365
HpyAV CCTTC 2 cut(s) 487, 517
HpyCH4III ACNGT 1 cut(s) 614
HpyCH4IV ACGT 2 cut(s) 170, 386
HpyCH4V TGCA 1 cut(s) 789
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 374, 458
HpyF3I CTNAG 3 cut(s) 34, 573, 809
HpySE526I ACGT 2 cut(s) 170, 386
Hsp92I GRCGYC 2 cut(s) 189, 621
Hsp92II CATG 2 cut(s) 153, 202
HspAI GCGC 1 cut(s) 145
KflI GGGWCCC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 239
Kzo9I GATC 3 cut(s) 112, 331, 745
LmnI GCTCC 5 cut(s) 264, 324, 336, 362, 430
Lsp1109I GCAGC 3 cut(s) 339, 341, 361
LweI GCATC 5 cut(s) 439, 470, 559, 655, 766
MaeI CTAG 2 cut(s) 104, 480
MaeII ACGT 2 cut(s) 170, 386
MaeIII GTNAC 1 cut(s) 608
MalI GATC 3 cut(s) 114, 333, 747
MbiI CCGCTC 1 cut(s) 339
MboI GATC 3 cut(s) 112, 331, 745
MboII GAAGA 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 2 cut(s) 454, 484
MlyI GAGTC 4 cut(s) 14, 101, 205, 413
MmeI TCCRAC 2 cut(s) 183, 302
MroI TCCGGA 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 737
MslI CAYNNNNRTG 2 cut(s) 197, 811
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 9 cut(s) 205, 229, 240, 346, 401, 536, 554, 588, 605
MspR9I CCNGG 9 cut(s) 17, 205, 283, 307, 346, 537, 555, 589, 606
MvaI CCWGG 3 cut(s) 17, 283, 307
MwoI GCNNNNNNNGC 3 cut(s) 336, 374, 458
NciI CCSGG 6 cut(s) 205, 346, 537, 555, 589, 606
NdeII GATC 3 cut(s) 112, 331, 745
NlaIII CATG 2 cut(s) 153, 202
NlaIV GGNNCC 4 cut(s) 248, 304, 343, 344
NmeAIII GCCGAG 1 cut(s) 649
NspI RCATGY 1 cut(s) 202
OliI CACNNNNGTG 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 421
PciI ACATGT 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 4 cut(s) 38, 273, 584, 803
PkrI GCNGC 5 cut(s) 294, 297, 329, 356, 376
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 4 cut(s) 14, 101, 205, 413
PpsI GAGTC 4 cut(s) 14, 101, 205, 413
PpuMI RGGWCCY 1 cut(s) 342
PscI ACATGT 1 cut(s) 198
PshAI GACNNNNGTC 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 15, 281, 305
PspEI GGTNACC 1 cut(s) 608
PspGI CCWGG 3 cut(s) 15, 281, 305
PspN4I GGNNCC 4 cut(s) 248, 304, 343, 344
PspPI GGNCC 5 cut(s) 27, 231, 303, 342, 551
PspPPI RGGWCCY 1 cut(s) 342
PspXI VCTCGAGB 1 cut(s) 421
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
RseI CAYNNNNRTG 2 cut(s) 197, 811
SalI GTCGAC 1 cut(s) 218
SatI GCNGC 5 cut(s) 293, 296, 328, 355, 375
Sau3AI GATC 3 cut(s) 112, 331, 745
Sau96I GGNCC 5 cut(s) 27, 231, 303, 342, 551
SchI GAGTC 4 cut(s) 14, 101, 205, 413
ScrFI CCNGG 9 cut(s) 17, 205, 283, 307, 346, 537, 555, 589, 606
SduI GDGCHC 1 cut(s) 249
SfaNI GCATC 5 cut(s) 439, 470, 559, 655, 766
Sfr274I CTCGAG 1 cut(s) 421
SinI GGWCC 3 cut(s) 231, 303, 342
SlaI CTCGAG 1 cut(s) 421
SmiMI CAYNNNNRTG 2 cut(s) 197, 811
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 2 cut(s) 454, 484
SsiI CCGC 5 cut(s) 117, 292, 295, 339, 372
SspMI CTAG 2 cut(s) 104, 480
StyD4I CCNGG 9 cut(s) 15, 203, 281, 305, 344, 535, 553, 587, 604
TaaI ACNGT 1 cut(s) 614
TaiI ACGT 2 cut(s) 173, 389
TaqI TCGA 6 cut(s) 186, 195, 219, 422, 691, 801
TasI AATT 2 cut(s) 454, 484
TatI WGTACW 1 cut(s) 44
TauI GCSGC 2 cut(s) 295, 298
TfiI GAWTC 4 cut(s) 38, 273, 584, 803
TscAI CASTG 1 cut(s) 813
TseI GCWGC 3 cut(s) 327, 354, 374
TspDTI ATGAA 2 cut(s) 597, 657
TspGWI ACGGA 2 cut(s) 349, 431
TspRI CASTG 1 cut(s) 813
VpaK11BI GGWCC 3 cut(s) 231, 303, 342
XapI RAATTY 1 cut(s) 484
XceI RCATGY 1 cut(s) 202
XhoI CTCGAG 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 2 cut(s) 104, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.