pycom03g22790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009253
Physical Location & Seq
Forward (+)
22821696 .. 22823204
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22790.2

Sequence Viewer

Length: 627 bp
ATGCCATCGACATGTCCCGGCTTACCTTGGTCGACGACGACAGGATCGATCTCCGGCGGACACCATACTAGGAACGGGAGCTTCCGGTTCATCGCGAAGAAGCGGCGGCGTCGTGGGTCGAGGTCGGTTTCCGGGAGGAGTAGTGAGCGGAGTGGGACCCGGAGGTGCTGCTCGGTTGGCGCTTCGGCGGCGTATGGGACGTGTTCGGATTTTCCGGTGGCGGTGGGGACGGACTCGAGTGGGGAGCTGTTTGGGAATGGGGATGCGAATTGGGCGTCGGATGTGAGTGAGGCGAGGAATTCGAGGAAGGAGAGAGATGGAGGTGGGGGGAGTGGAGAGAAGGAGAATATGGGTGTTGGGTTTGGTCCCATTGGGGGTTTTGATGCTCAGGGGAATGAGTCCGGGTACGGCAGTGAACCAGGTTATCGCGGGGACGCAGAGTTCGGCTATGGCGATGAGTTTGATGAGGAGGAGGAGGATACTCGGGTATTGTTTTGGGGCGATCAATTTGGAGATGCTGATTCTATGATGGAGACAGTGGGGGAGAATACATTCGCGGATCAAAAGTCCCATCACAGATGTCGGCGTAAGAAACACGATTGCAGAATGGTTGATGCACTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.41

Weight (kDa)

5.78

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 32
AccII CGCG 3 cut(s) 95, 429, 557
AciI CCGC 8 cut(s) 57, 103, 106, 148, 188, 221, 429, 557
AclWI GGATC 2 cut(s) 52, 567
AcsI RAATTY 1 cut(s) 298
AcyI GRCGYC 2 cut(s) 109, 275
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 374
AflIII ACRYGT 2 cut(s) 11, 200
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 1 cut(s) 418
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 2 cut(s) 81, 247
AluI AGCT 2 cut(s) 81, 247
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 2 cut(s) 52, 567
Ama87I CYCGRG 2 cut(s) 235, 483
ApeKI GCWGC 1 cut(s) 168
ApoI RAATTY 1 cut(s) 298
ArsI GACNNNNNNTTYG 4 cut(s) 260, 292, 425, 457
Asp700I GAANNNNTTC 1 cut(s) 551
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 2 cut(s) 156, 365
AsuC2I CCSGG 4 cut(s) 18, 133, 160, 403
AvaI CYCGRG 2 cut(s) 235, 483
AvaII GGWCC 2 cut(s) 156, 365
BaeI ACNNNNGTAYC 4 cut(s) 397, 430, 471, 504
BbvI GCAGC 1 cut(s) 155
BccI CCATC 4 cut(s) 13, 311, 523, 579
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 1 cut(s) 472
BcnI CCSGG 4 cut(s) 18, 133, 160, 403
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 183
BfuI GTATCC 1 cut(s) 472
BisI GCNGC 4 cut(s) 104, 107, 169, 189
BlsI GCNGC 4 cut(s) 105, 108, 170, 190
Bme1390I CCNGG 5 cut(s) 18, 133, 160, 403, 420
Bme18I GGWCC 2 cut(s) 156, 365
BmeT110I CYCGRG 2 cut(s) 235, 483
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 2 cut(s) 156, 365
BmiI GGNNCC 3 cut(s) 157, 158, 367
BmrFI CCNGG 5 cut(s) 18, 133, 160, 403, 420
BmsI GCATC 4 cut(s) 253, 373, 505, 604
Bpu10I CCTNAGC 1 cut(s) 387
BpuMI CCSGG 4 cut(s) 18, 133, 160, 403
Bsa29I ATCGAT 1 cut(s) 47
BsaHI GRCGYC 2 cut(s) 109, 275
BsaJI CCNNGG 1 cut(s) 26
BsaWI WCCGGW 2 cut(s) 84, 214
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 1 cut(s) 374
BseBI CCWGG 1 cut(s) 420
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 2 cut(s) 268, 286
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMII CTCAG 2 cut(s) 401, 611
BseRI GAGGAG 4 cut(s) 151, 482, 485, 488
BseXI GCAGC 1 cut(s) 155
Bsh1236I CGCG 3 cut(s) 95, 429, 557
BshVI ATCGAT 1 cut(s) 47
BsiHKCI CYCGRG 2 cut(s) 235, 483
BsiSI CCGG 7 cut(s) 18, 54, 85, 132, 160, 215, 402
BslFI GGGAC 6 cut(s) 169, 211, 241, 351, 446, 553
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 527
BsmFI GGGAC 6 cut(s) 169, 211, 241, 351, 446, 553
BsoBI CYCGRG 2 cut(s) 235, 483
Bsp143I GATC 4 cut(s) 44, 48, 502, 559
Bsp68I TCGCGA 1 cut(s) 95
BspACI CCGC 8 cut(s) 57, 103, 106, 148, 188, 221, 429, 557
BspCNI CTCAG 2 cut(s) 400, 612
BspDI ATCGAT 1 cut(s) 47
BspFNI CGCG 3 cut(s) 95, 429, 557
BspLI GGNNCC 3 cut(s) 157, 158, 367
BspPI GGATC 2 cut(s) 52, 567
BsrBI CCGCTC 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 4 cut(s) 44, 48, 502, 559
BssNI GRCGYC 2 cut(s) 109, 275
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 538
BstACI GRCGYC 2 cut(s) 109, 275
BstDEI CTNAG 2 cut(s) 387, 620
BstF5I GGATG 2 cut(s) 268, 286
BstFNI CGCG 3 cut(s) 95, 429, 557
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 4 cut(s) 47, 51, 505, 562
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 4 cut(s) 44, 48, 502, 559
BstMWI GCNNNNNNNGC 3 cut(s) 177, 188, 272
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 15
BstSCI CCNGG 5 cut(s) 16, 131, 158, 401, 418
BstUI CGCG 3 cut(s) 95, 429, 557
BstV1I GCAGC 1 cut(s) 155
Bsu15I ATCGAT 1 cut(s) 47
BsuI GTATCC 1 cut(s) 472
BsuTUI ATCGAT 1 cut(s) 47
BtgZI GCGATG 2 cut(s) 76, 468
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 2 cut(s) 268, 286
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 3 cut(s) 418, 543, 617
BtuMI TCGCGA 1 cut(s) 95
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 156, 365
ClaI ATCGAT 1 cut(s) 47
CseI GACGC 3 cut(s) 98, 264, 443
CsiI ACCWGGT 1 cut(s) 418
Csp6I GTAC 1 cut(s) 406
CviAII CATG 1 cut(s) 12
CviJI RGCY 4 cut(s) 21, 81, 247, 447
CviKI_1 RGCY 4 cut(s) 21, 81, 247, 447
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 2 cut(s) 387, 620
DpnI GATC 4 cut(s) 46, 50, 504, 561
DpnII GATC 4 cut(s) 44, 48, 502, 559
EciI GGCGGA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 156, 365
Eco88I CYCGRG 2 cut(s) 235, 483
EcoO109I RGGNCCY 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 1 cut(s) 15
FaiI YATR 6 cut(s) 13, 66, 195, 350, 450, 527
FaqI GGGAC 6 cut(s) 169, 211, 241, 351, 446, 553
FatI CATG 1 cut(s) 11
FauI CCCGC 1 cut(s) 422
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 104, 107, 169, 189
FokI GGATG 2 cut(s) 275, 293
Fsp4HI GCNGC 4 cut(s) 104, 107, 169, 189
FspBI CTAG 1 cut(s) 69
GlaI GCGC 1 cut(s) 181
GluI GCNGC 4 cut(s) 104, 107, 169, 189
HaeII RGCGCY 1 cut(s) 183
HapII CCGG 7 cut(s) 18, 54, 85, 132, 160, 215, 402
HgaI GACGC 3 cut(s) 98, 264, 443
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 2 cut(s) 109, 275
Hin1II CATG 1 cut(s) 15
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 3 cut(s) 233, 398, 521
HpaII CCGG 7 cut(s) 18, 54, 85, 132, 160, 215, 402
Hpy166II GTNNAC 2 cut(s) 33, 416
Hpy188I TCNGA 2 cut(s) 208, 280
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 2 cut(s) 33, 416
Hpy99I CGWCG 4 cut(s) 37, 40, 114, 280
HpyAV CCTTC 2 cut(s) 301, 334
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 2 cut(s) 603, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 188, 272
HpyF3I CTNAG 2 cut(s) 387, 620
HpySE526I ACGT 1 cut(s) 200
Hsp92I GRCGYC 2 cut(s) 109, 275
Hsp92II CATG 1 cut(s) 15
HspAI GCGC 1 cut(s) 180
KflI GGGWCCC 1 cut(s) 156
Kzo9I GATC 4 cut(s) 44, 48, 502, 559
LmnI GCTCC 2 cut(s) 78, 244
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 4 cut(s) 253, 373, 505, 604
MabI ACCWGGT 1 cut(s) 418
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 200
MalI GATC 4 cut(s) 46, 50, 504, 561
MbiI CCGCTC 1 cut(s) 148
MboI GATC 4 cut(s) 44, 48, 502, 559
MboII GAAGA 1 cut(s) 109
MluCI AATT 3 cut(s) 268, 298, 506
MlyI GAGTC 2 cut(s) 227, 407
MmeI TCCRAC 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 551
MslI CAYNNNNRTG 1 cut(s) 10
MspI CCGG 7 cut(s) 18, 54, 85, 132, 160, 215, 402
MspR9I CCNGG 5 cut(s) 18, 133, 160, 403, 420
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 3 cut(s) 95, 429, 557
MwoI GCNNNNNNNGC 3 cut(s) 177, 188, 272
NciI CCSGG 4 cut(s) 18, 133, 160, 403
NdeII GATC 4 cut(s) 44, 48, 502, 559
NlaIII CATG 1 cut(s) 15
NlaIV GGNNCC 3 cut(s) 157, 158, 367
NruI TCGCGA 1 cut(s) 95
NspI RCATGY 1 cut(s) 15
PaeR7I CTCGAG 1 cut(s) 235
PciI ACATGT 1 cut(s) 11
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 450
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 1 cut(s) 521
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 4 cut(s) 105, 108, 170, 190
PleI GAGTC 2 cut(s) 227, 406
PpsI GAGTC 2 cut(s) 227, 406
PpuMI RGGWCCY 1 cut(s) 156
PscI ACATGT 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 3 cut(s) 157, 158, 367
PspPI GGNCC 2 cut(s) 156, 365
PspPPI RGGWCCY 1 cut(s) 156
PspXI VCTCGAGB 1 cut(s) 235
RruI TCGCGA 1 cut(s) 95
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 10
SalI GTCGAC 1 cut(s) 31
SatI GCNGC 4 cut(s) 104, 107, 169, 189
Sau3AI GATC 4 cut(s) 44, 48, 502, 559
Sau96I GGNCC 2 cut(s) 156, 365
SchI GAGTC 2 cut(s) 227, 407
ScrFI CCNGG 5 cut(s) 18, 133, 160, 403, 420
SetI ASST 9 cut(s) 28, 83, 125, 167, 203, 249, 325, 424, 626
SexAI ACCWGGT 1 cut(s) 418
SfaNI GCATC 4 cut(s) 253, 373, 505, 604
Sfr274I CTCGAG 1 cut(s) 235
SinI GGWCC 2 cut(s) 156, 365
SlaI CTCGAG 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 235
SmoI CTYRAG 1 cut(s) 235
Sse9I AATT 3 cut(s) 268, 298, 506
SsiI CCGC 8 cut(s) 57, 103, 106, 148, 188, 221, 429, 557
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 5 cut(s) 16, 131, 158, 401, 418
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 203
TaqI TCGA 6 cut(s) 8, 32, 47, 119, 236, 302
TasI AATT 3 cut(s) 268, 298, 506
TauI GCSGC 3 cut(s) 106, 109, 191
TfiI GAWTC 1 cut(s) 521
TscAI CASTG 3 cut(s) 418, 543, 624
TseI GCWGC 1 cut(s) 168
TspDTI ATGAA 1 cut(s) 79
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 3 cut(s) 418, 543, 624
VpaK11BI GGWCC 2 cut(s) 156, 365
XapI RAATTY 1 cut(s) 298
XceI RCATGY 1 cut(s) 15
XhoI CTCGAG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.