MD03G1287800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009253
Physical Location & Seq
Forward (+)
36674456 .. 36676582
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1287800.v1.1.491

Sequence Viewer

Length: 837 bp
ATGTCACACAAATCCCTGGATTCACGGCACTCCGTCGATTCCTGCGCGTTCCAGCTCCATGGCTGGAGACCTTTCCAGCTGCAGCAGCAGCAGCATCAGACCTCCACGCCCACCTCCAAAACCCTAGACTCCTCCGATCCCAAATCCAATGGCCTCGTCGTCCACACCAAGCGCCCCTGCCTCTCCAACCGGATGACTTCTTTTTCTATCGATGCCGTCGACATGTCCCGGCTGACCTTGGTCGACGACGACAGGACGATCTCCGGCGGACACCATACTAGGAACGGGAGCTTCCGGTTCATCGCGAAGAAGCGGCGGCGTCGTGGGTCGAGGTCGGTTTCCGGCAGGAGTAGTGAGCGGAGTGGGACCCGGAGGTGCTGCTCGGTTGGGGCTTCGGCGGCGTATGGGACGTGTTCGGATTTTCCGGTGGCGGTGGGGACGGACTCGAGTGGGGAGCTGTTTGGGAATGGGGATGCGAATTGGGCGTCGGATGTGAGTGAGGCGAGGAATTCGAGGAAGGAGAGAGATGGCGGTGGGGGGAGTGGAGAGAAGGAGAATGTGGGTGTTGGGTTTGGTCCCATCGGGGGTATTGATGCTCAGGGGAATGAGTCCGGGTACGGCAGTGAACCGGGTTATCGTGGGGACGCGGAGTTAGGCTATGGCGATGAGTTTGATGAGGAGGAGGAGGATACTCGGGTATTGTTTTGGAGCGATCAATTTGGAGATGCTGATTCTATGATGGAGACAGTGGGGGAGAACACATTTGCGGATCAAAAGTCCCATCACAGATGTCGGCGTAAGAAGCACGATTGCAGAATGGTTGATGCACTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

30.44

Weight (kDa)

6.37

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 2 cut(s) 219, 243
AccII CGCG 3 cut(s) 47, 305, 647
AciI CCGC 9 cut(s) 267, 313, 316, 358, 398, 431, 531, 647, 767
AclWI GGATC 2 cut(s) 131, 777
AcsI RAATTY 1 cut(s) 508
AcyI GRCGYC 2 cut(s) 319, 485
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 2 cut(s) 222, 410
AjiI CACGTC 1 cut(s) 411
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 4 cut(s) 55, 79, 291, 457
AluI AGCT 4 cut(s) 55, 79, 291, 457
Alw26I GTCTC 2 cut(s) 61, 737
AlwI GGATC 2 cut(s) 131, 777
Ama87I CYCGRG 2 cut(s) 445, 693
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 6 cut(s) 79, 82, 85, 88, 91, 378
ApoI RAATTY 1 cut(s) 508
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AspLEI GCGC 2 cut(s) 47, 174
AspS9I GGNCC 2 cut(s) 366, 575
AsuC2I CCSGG 4 cut(s) 229, 370, 613, 630
AvaI CYCGRG 2 cut(s) 445, 693
AvaII GGWCC 2 cut(s) 366, 575
BaeI ACNNNNGTAYC 4 cut(s) 607, 640, 681, 714
BbvI GCAGC 6 cut(s) 66, 94, 97, 100, 103, 365
BccI CCATC 4 cut(s) 521, 587, 733, 789
BceAI ACGGC 3 cut(s) 41, 200, 634
BciT130I CCWGG 1 cut(s) 17
BciVI GTATCC 1 cut(s) 682
BcnI CCSGG 4 cut(s) 229, 370, 613, 630
BcoDI GTCTC 2 cut(s) 61, 737
BfaI CTAG 2 cut(s) 125, 279
BfmI CTRYAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 175
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 9 cut(s) 80, 83, 86, 89, 92, 314, 317, 379, 399
BlsI GCNGC 9 cut(s) 81, 84, 87, 90, 93, 315, 318, 380, 400
Bme1390I CCNGG 5 cut(s) 17, 229, 370, 613, 630
Bme18I GGWCC 2 cut(s) 366, 575
BmeT110I CYCGRG 2 cut(s) 445, 693
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 2 cut(s) 366, 575
BmiI GGNNCC 3 cut(s) 367, 368, 577
BmrFI CCNGG 5 cut(s) 17, 229, 370, 613, 630
BmsI GCATC 6 cut(s) 103, 202, 463, 583, 715, 814
BoxI GACNNNNGTC 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 597
BpuMI CCSGG 4 cut(s) 229, 370, 613, 630
Bsa29I ATCGAT 1 cut(s) 210
BsaHI GRCGYC 2 cut(s) 319, 485
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 15, 58, 237
BsaWI WCCGGW 3 cut(s) 189, 294, 424
Bsc4I CCNNNNNNNGG 1 cut(s) 584
BseBI CCWGG 1 cut(s) 17
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 15, 58, 237
BseGI GGATG 3 cut(s) 198, 478, 496
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 2 cut(s) 611, 821
BseRI GAGGAG 4 cut(s) 121, 692, 695, 698
BseXI GCAGC 6 cut(s) 66, 94, 97, 100, 103, 365
Bsh1236I CGCG 3 cut(s) 47, 305, 647
BshFI GGCC 1 cut(s) 153
BshVI ATCGAT 1 cut(s) 210
BsiHKCI CYCGRG 2 cut(s) 445, 693
BsiSI CCGG 9 cut(s) 190, 229, 264, 295, 342, 370, 425, 612, 629
BslFI GGGAC 7 cut(s) 211, 379, 421, 451, 561, 656, 763
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 2 cut(s) 61, 737
BsmFI GGGAC 7 cut(s) 211, 379, 421, 451, 561, 656, 763
BsnI GGCC 1 cut(s) 153
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 2 cut(s) 445, 693
Bsp143I GATC 4 cut(s) 136, 258, 712, 769
Bsp19I CCATGG 1 cut(s) 58
Bsp68I TCGCGA 1 cut(s) 305
BspACI CCGC 9 cut(s) 267, 313, 316, 358, 398, 431, 531, 647, 767
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 2 cut(s) 610, 822
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 3 cut(s) 47, 305, 647
BspLI GGNNCC 3 cut(s) 367, 368, 577
BspMAI CTGCAG 1 cut(s) 84
BspPI GGATC 2 cut(s) 131, 777
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 15, 58, 237
BssMI GATC 4 cut(s) 136, 258, 712, 769
BssNI GRCGYC 2 cut(s) 319, 485
BssT1I CCWWGG 2 cut(s) 58, 237
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 748
BstACI GRCGYC 2 cut(s) 319, 485
BstDEI CTNAG 2 cut(s) 597, 830
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 3 cut(s) 198, 478, 496
BstFNI CGCG 3 cut(s) 47, 305, 647
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 2 cut(s) 47, 174
BstKTI GATC 4 cut(s) 139, 261, 715, 772
BstMAI GTCTC 2 cut(s) 61, 737
BstMBI GATC 4 cut(s) 136, 258, 712, 769
BstMWI GCNNNNNNNGC 6 cut(s) 85, 88, 91, 398, 482, 802
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 226
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 5 cut(s) 15, 227, 368, 611, 628
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 3 cut(s) 47, 305, 647
BstV1I GCAGC 6 cut(s) 66, 94, 97, 100, 103, 365
BstXI CCANNNNNNTGG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 1 cut(s) 153
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 58
BtgZI GCGATG 2 cut(s) 286, 678
BtrI CACGTC 1 cut(s) 411
BtsCI GGATG 3 cut(s) 198, 478, 496
BtsI GCAGTG 1 cut(s) 628
BtsIMutI CAGTG 3 cut(s) 628, 753, 827
BtuMI TCGCGA 1 cut(s) 305
CfoI GCGC 2 cut(s) 47, 174
Cfr13I GGNCC 2 cut(s) 366, 575
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 3 cut(s) 308, 474, 653
Csp6I GTAC 1 cut(s) 616
CviAII CATG 2 cut(s) 59, 223
CviJI RGCY 9 cut(s) 55, 63, 79, 153, 232, 291, 392, 457, 657
CviKI_1 RGCY 9 cut(s) 55, 63, 79, 153, 232, 291, 392, 457, 657
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 2 cut(s) 597, 830
DpnI GATC 4 cut(s) 138, 260, 714, 771
DpnII GATC 4 cut(s) 136, 258, 712, 769
EciI GGCGGA 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 58, 237
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 366, 575
Eco88I CYCGRG 2 cut(s) 445, 693
EcoO109I RGGNCCY 1 cut(s) 366
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 15
EcoT14I CCWWGG 2 cut(s) 58, 237
ErhI CCWWGG 2 cut(s) 58, 237
FaeI CATG 2 cut(s) 62, 226
FaiI YATR 6 cut(s) 60, 224, 276, 405, 660, 737
FaqI GGGAC 7 cut(s) 211, 379, 421, 451, 561, 656, 763
FatI CATG 2 cut(s) 58, 222
FblI GTMKAC 2 cut(s) 219, 243
Fnu4HI GCNGC 9 cut(s) 80, 83, 86, 89, 92, 314, 317, 379, 399
FokI GGATG 3 cut(s) 205, 485, 503
Fsp4HI GCNGC 9 cut(s) 80, 83, 86, 89, 92, 314, 317, 379, 399
FspBI CTAG 2 cut(s) 125, 279
GlaI GCGC 2 cut(s) 46, 173
GluI GCNGC 9 cut(s) 80, 83, 86, 89, 92, 314, 317, 379, 399
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 1 cut(s) 153
HapII CCGG 9 cut(s) 190, 229, 264, 295, 342, 370, 425, 612, 629
HgaI GACGC 3 cut(s) 308, 474, 653
HhaI GCGC 2 cut(s) 47, 174
Hin1I GRCGYC 2 cut(s) 319, 485
Hin1II CATG 2 cut(s) 62, 226
Hin6I GCGC 2 cut(s) 45, 172
HinP1I GCGC 2 cut(s) 45, 172
HincII GTYRAC 2 cut(s) 220, 244
HindII GTYRAC 2 cut(s) 220, 244
HinfI GANTC 6 cut(s) 20, 38, 128, 443, 608, 731
HpaII CCGG 9 cut(s) 190, 229, 264, 295, 342, 370, 425, 612, 629
Hpy166II GTNNAC 4 cut(s) 163, 220, 244, 626
Hpy188I TCNGA 4 cut(s) 99, 136, 418, 490
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 4 cut(s) 163, 220, 244, 626
Hpy99I CGWCG 7 cut(s) 38, 161, 221, 248, 251, 324, 490
HpyAV CCTTC 2 cut(s) 511, 544
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 3 cut(s) 82, 813, 827
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 88, 91, 398, 482, 802
HpyF3I CTNAG 2 cut(s) 597, 830
HpySE526I ACGT 1 cut(s) 410
Hsp92I GRCGYC 2 cut(s) 319, 485
Hsp92II CATG 2 cut(s) 62, 226
HspAI GCGC 2 cut(s) 45, 172
KflI GGGWCCC 1 cut(s) 366
Kzo9I GATC 4 cut(s) 136, 258, 712, 769
LmnI GCTCC 4 cut(s) 60, 288, 454, 708
Lsp1109I GCAGC 6 cut(s) 66, 94, 97, 100, 103, 365
LweI GCATC 6 cut(s) 103, 202, 463, 583, 715, 814
MaeI CTAG 2 cut(s) 125, 279
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 3
MalI GATC 4 cut(s) 138, 260, 714, 771
MbiI CCGCTC 1 cut(s) 358
MboI GATC 4 cut(s) 136, 258, 712, 769
MboII GAAGA 1 cut(s) 319
MluCI AATT 3 cut(s) 478, 508, 716
MlyI GAGTC 3 cut(s) 122, 437, 617
MmeI TCCRAC 2 cut(s) 210, 468
MspA1I CMGCKG 1 cut(s) 79
MspI CCGG 9 cut(s) 190, 229, 264, 295, 342, 370, 425, 612, 629
MspR9I CCNGG 5 cut(s) 17, 229, 370, 613, 630
MvaI CCWGG 1 cut(s) 17
MvnI CGCG 3 cut(s) 47, 305, 647
MwoI GCNNNNNNNGC 6 cut(s) 85, 88, 91, 398, 482, 802
NciI CCSGG 4 cut(s) 229, 370, 613, 630
NcoI CCATGG 1 cut(s) 58
NdeII GATC 4 cut(s) 136, 258, 712, 769
NlaIII CATG 2 cut(s) 62, 226
NlaIV GGNNCC 3 cut(s) 367, 368, 577
NmuCI GTSAC 1 cut(s) 3
NruI TCGCGA 1 cut(s) 305
NspI RCATGY 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 445
PciI ACATGT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 3 cut(s) 20, 38, 731
PkrI GCNGC 9 cut(s) 81, 84, 87, 90, 93, 315, 318, 380, 400
PleI GAGTC 3 cut(s) 122, 437, 616
PpsI GAGTC 3 cut(s) 122, 437, 616
PpuMI RGGWCCY 1 cut(s) 366
PscI ACATGT 1 cut(s) 222
PshAI GACNNNNGTC 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 3 cut(s) 367, 368, 577
PspPI GGNCC 2 cut(s) 366, 575
PspPPI RGGWCCY 1 cut(s) 366
PspXI VCTCGAGB 1 cut(s) 445
PstI CTGCAG 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 79
RruI TCGCGA 1 cut(s) 305
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
SalI GTCGAC 2 cut(s) 218, 242
SatI GCNGC 9 cut(s) 80, 83, 86, 89, 92, 314, 317, 379, 399
Sau3AI GATC 4 cut(s) 136, 258, 712, 769
Sau96I GGNCC 2 cut(s) 366, 575
SchI GAGTC 3 cut(s) 122, 437, 617
ScrFI CCNGG 5 cut(s) 17, 229, 370, 613, 630
SfaNI GCATC 6 cut(s) 103, 202, 463, 583, 715, 814
SfcI CTRYAG 1 cut(s) 80
Sfr274I CTCGAG 1 cut(s) 445
SinI GGWCC 2 cut(s) 366, 575
SlaI CTCGAG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
Sse9I AATT 3 cut(s) 478, 508, 716
SsiI CCGC 9 cut(s) 267, 313, 316, 358, 398, 431, 531, 647, 767
SspMI CTAG 2 cut(s) 125, 279
StyD4I CCNGG 5 cut(s) 15, 227, 368, 611, 628
StyI CCWWGG 2 cut(s) 58, 237
TaaI ACNGT 1 cut(s) 748
TaiI ACGT 1 cut(s) 413
TaqI TCGA 7 cut(s) 36, 210, 219, 243, 329, 446, 512
TasI AATT 3 cut(s) 478, 508, 716
TauI GCSGC 3 cut(s) 316, 319, 401
TfiI GAWTC 3 cut(s) 20, 38, 731
TscAI CASTG 3 cut(s) 628, 753, 834
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 6 cut(s) 79, 82, 85, 88, 91, 378
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 289
TspGWI ACGGA 2 cut(s) 22, 455
TspRI CASTG 3 cut(s) 628, 753, 834
VpaK11BI GGWCC 2 cut(s) 366, 575
XapI RAATTY 1 cut(s) 508
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 445
XmiI GTMKAC 2 cut(s) 219, 243
XspI CTAG 2 cut(s) 125, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.