Rh6DG353300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009253
Physical Location & Seq
Forward (+)
55286740 .. 55287549
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG353300.1

Sequence Viewer

Length: 810 bp
ATGTCACGTTCCCACAAAGCCCTCGACTCACGGCACCCCTTAGATTCGTGTACATTTCAACTCCATAGCTGGAGACCTTTCCAGCTTCAACAGCAGACCACCACCGCACCCACCTCCAAAACCCTAGACTCCGATCCCACCAACCCCAAACCCTACTACACCAAGCGTCCATGCCTCTCCAACCGAGCGACGTCGTTTTCCATCGACGCCATCGACATGTCCAGGCTGACTTTGGTCGACGACGACCGGACGATCTCCGGAGGGCACCATAAGCACGGGAGCTTCCGATTCATTGCCAAGAAGAGGCGGCGGCGCGGGTCCAGGTCGGTTTCGGGTCGGAGCAGCGATCGGAGCGGGACCCGGAGGTGCTGCTCCGTCGGGGCTTCGGCTGCTTACGGGACGTGTTCGGATTTTCCGGTGGCGATTGGTACGGACTCGAGTGGGGAGCTGTTTGGGAATGGGGATGCGAATTGGGCCTCGGATGTGAGTGAGGCTAGGAATTCGAGGAAGGAGAGGGATGGAGTTGGGAGTGGAGAGAAGGAGAATCCGGGTGTCGGGTTTGGGCCGAGTGGGGGCTTTGATGCTCAGGGAAATGAGTCCGGATATGGTAGTGAACCGGGTTACCGTGGGGACGCGGAGTTCGGATATGGTGATGAACTTGATGAGGAGGAGGATGATGCTCGTCTATTGTTTTGGGGCAATCATTTCGGAGGTACATTTTGTCCTTGTTGCATTTCGAGCAGTGTTGATATCATTGTCAACTATAATGTAACATTTGATACATCTGTTTCACCCGGCGTTCTGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

269

Amino Acids

29.15

Weight (kDa)

5.98

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 33, 264
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 315, 635
AccIII TCCGGA 2 cut(s) 257, 599
AciI CCGC 6 cut(s) 105, 307, 310, 315, 354, 635
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 499
AcyI GRCGYC 2 cut(s) 191, 207
AfaI GTAC 3 cut(s) 52, 430, 715
AfiI CCNNNNNNNGG 3 cut(s) 303, 554, 572
AflIII ACRYGT 2 cut(s) 216, 401
AgsI TTSAA 2 cut(s) 59, 89
AjiI CACGTC 1 cut(s) 402
AjnI CCWGG 2 cut(s) 221, 320
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 4 cut(s) 69, 85, 282, 448
AluI AGCT 4 cut(s) 69, 85, 282, 448
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 436
Aor13HI TCCGGA 2 cut(s) 257, 599
AoxI GGCC 2 cut(s) 474, 563
ApeKI GCWGC 3 cut(s) 342, 369, 389
ApoI RAATTY 1 cut(s) 499
ArsI GACNNNNNNTTYG 2 cut(s) 623, 655
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 4 cut(s) 318, 357, 474, 563
AsuC2I CCSGG 4 cut(s) 361, 549, 618, 795
AsuHPI GGTGA 2 cut(s) 662, 783
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 318, 357
BaeGI GKGCMC 1 cut(s) 267
BaeI ACNNNNGTAYC 2 cut(s) 771, 804
BanI GGYRCC 2 cut(s) 33, 264
BbvI GCAGC 3 cut(s) 354, 356, 376
BccI CCATC 3 cut(s) 209, 218, 512
BceAI ACGGC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 2 cut(s) 223, 322
BcnI CCSGG 4 cut(s) 361, 549, 618, 795
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 2 cut(s) 125, 495
BfmI CTRYAG 1 cut(s) 806
BisI GCNGC 5 cut(s) 308, 311, 343, 370, 390
BlsI GCNGC 5 cut(s) 309, 312, 344, 371, 391
Bme1390I CCNGG 6 cut(s) 223, 322, 361, 549, 618, 795
Bme18I GGWCC 2 cut(s) 318, 357
BmeT110I CYCGRG 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 402
BmgT120I GGNCC 4 cut(s) 318, 357, 474, 563
BmiI GGNNCC 5 cut(s) 35, 266, 319, 358, 359
BmrFI CCNGG 6 cut(s) 223, 322, 361, 549, 618, 795
BmsI GCATC 3 cut(s) 454, 571, 667
BoxI GACNNNNGTC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 585
BpuMI CCSGG 4 cut(s) 361, 549, 618, 795
BsaHI GRCGYC 2 cut(s) 191, 207
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 477, 625
BsaWI WCCGGW 4 cut(s) 246, 257, 415, 599
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 554, 572
Bse3DI GCAATG 1 cut(s) 291
BseAI TCCGGA 2 cut(s) 257, 599
BseBI CCWGG 2 cut(s) 223, 322
BseDI CCNNGG 2 cut(s) 477, 625
BseGI GGATG 4 cut(s) 469, 487, 523, 679
BseLI CCNNNNNNNGG 3 cut(s) 303, 554, 572
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 599
BseRI GAGGAG 2 cut(s) 680, 683
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 3 cut(s) 354, 356, 376
Bsh1236I CGCG 2 cut(s) 315, 635
Bsh1285I CGRYCG 2 cut(s) 247, 349
BshFI GGCC 2 cut(s) 476, 565
BshNI GGYRCC 2 cut(s) 33, 264
BsiEI CGRYCG 2 cut(s) 247, 349
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 8 cut(s) 247, 258, 361, 416, 548, 600, 617, 795
BslFI GGGAC 3 cut(s) 370, 412, 644
BslI CCNNNNNNNGG 3 cut(s) 303, 554, 572
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 3 cut(s) 370, 412, 644
BsnI GGCC 2 cut(s) 476, 565
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 267
Bsp13I TCCGGA 2 cut(s) 257, 599
Bsp1407I TGTACA 1 cut(s) 50
Bsp143I GATC 3 cut(s) 133, 252, 346
BspACI CCGC 6 cut(s) 105, 307, 310, 315, 354, 635
BspANI GGCC 2 cut(s) 476, 565
BspCNI CTCAG 1 cut(s) 598
BspEI TCCGGA 2 cut(s) 257, 599
BspFNI CGCG 2 cut(s) 315, 635
BspLI GGNNCC 5 cut(s) 35, 266, 319, 358, 359
BspPI GGATC 1 cut(s) 128
BspT107I GGYRCC 2 cut(s) 33, 264
BspTNI GGTCTC 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 291
BsrGI TGTACA 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 477, 625
BssMI GATC 3 cut(s) 133, 252, 346
BssNI GRCGYC 2 cut(s) 191, 207
Bst2UI CCWGG 2 cut(s) 223, 322
Bst4CI ACNGT 1 cut(s) 626
Bst6I CTCTTC 1 cut(s) 296
BstACI GRCGYC 2 cut(s) 191, 207
BstAUI TGTACA 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 40, 585
BstDSI CCRYGG 1 cut(s) 625
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 4 cut(s) 469, 487, 523, 679
BstFNI CGCG 2 cut(s) 315, 635
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 3 cut(s) 136, 255, 349
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 3 cut(s) 133, 252, 346
BstMCI CGRYCG 2 cut(s) 247, 349
BstMWI GCNNNNNNNGC 6 cut(s) 91, 271, 351, 389, 473, 738
BstNI CCWGG 2 cut(s) 223, 322
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 233
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 6 cut(s) 221, 320, 359, 547, 616, 793
BstSFI CTRYAG 1 cut(s) 806
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 2 cut(s) 315, 635
BstV1I GCAGC 3 cut(s) 354, 356, 376
BsuRI GGCC 2 cut(s) 476, 565
BtgI CCRYGG 1 cut(s) 625
BtrI CACGTC 1 cut(s) 402
BtsCI GGATG 4 cut(s) 469, 487, 523, 679
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 1 cut(s) 748
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 4 cut(s) 318, 357, 474, 563
CseI GACGC 3 cut(s) 155, 215, 641
Csp6I GTAC 3 cut(s) 51, 429, 714
CviAII CATG 2 cut(s) 171, 217
CviQI GTAC 3 cut(s) 51, 429, 714
DdeI CTNAG 2 cut(s) 40, 585
DpnI GATC 3 cut(s) 135, 254, 348
DpnII GATC 3 cut(s) 133, 252, 346
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 67
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 318, 357
Eco88I CYCGRG 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 357
EcoO65I GGTNACC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 2 cut(s) 221, 320
EcoRV GATATC 1 cut(s) 751
FaeI CATG 2 cut(s) 174, 220
FaiI YATR 8 cut(s) 66, 172, 218, 270, 606, 648, 765, 808
FaqI GGGAC 3 cut(s) 370, 412, 644
FatI CATG 2 cut(s) 170, 216
FauI CCCGC 2 cut(s) 308, 347
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 5 cut(s) 308, 311, 343, 370, 390
FokI GGATG 4 cut(s) 476, 494, 530, 686
Fsp4HI GCNGC 5 cut(s) 308, 311, 343, 370, 390
FspBI CTAG 2 cut(s) 125, 495
GlaI GCGC 1 cut(s) 314
GluI GCNGC 5 cut(s) 308, 311, 343, 370, 390
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 2 cut(s) 476, 565
HapII CCGG 8 cut(s) 247, 258, 361, 416, 548, 600, 617, 795
HgaI GACGC 3 cut(s) 155, 215, 641
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 2 cut(s) 191, 207
Hin1II CATG 2 cut(s) 174, 220
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HincII GTYRAC 2 cut(s) 238, 760
HindII GTYRAC 2 cut(s) 238, 760
HinfI GANTC 7 cut(s) 26, 44, 128, 288, 434, 544, 596
HpaII CCGG 8 cut(s) 247, 258, 361, 416, 548, 600, 617, 795
HphI GGTGA 2 cut(s) 662, 783
Hpy166II GTNNAC 4 cut(s) 51, 238, 614, 760
Hpy188I TCNGA 8 cut(s) 133, 287, 339, 351, 409, 481, 644, 710
Hpy188III TCNNGA 2 cut(s) 258, 600
Hpy8I GTNNAC 4 cut(s) 51, 238, 614, 760
Hpy99I CGWCG 6 cut(s) 193, 196, 209, 242, 245, 380
HpyAV CCTTC 2 cut(s) 502, 532
HpyCH4III ACNGT 1 cut(s) 626
HpyCH4IV ACGT 3 cut(s) 7, 191, 401
HpyCH4V TGCA 1 cut(s) 732
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 271, 351, 389, 473, 738
HpyF3I CTNAG 2 cut(s) 40, 585
HpySE526I ACGT 3 cut(s) 7, 191, 401
Hsp92I GRCGYC 2 cut(s) 191, 207
Hsp92II CATG 2 cut(s) 174, 220
HspAI GCGC 1 cut(s) 313
KflI GGGWCCC 1 cut(s) 357
Kpn2I TCCGGA 2 cut(s) 257, 599
Kzo9I GATC 3 cut(s) 133, 252, 346
LmnI GCTCC 5 cut(s) 279, 339, 351, 377, 445
Lsp1109I GCAGC 3 cut(s) 354, 356, 376
LweI GCATC 3 cut(s) 454, 571, 667
MaeI CTAG 2 cut(s) 125, 495
MaeII ACGT 3 cut(s) 7, 191, 401
MaeIII GTNAC 3 cut(s) 3, 620, 769
MalI GATC 3 cut(s) 135, 254, 348
MbiI CCGCTC 1 cut(s) 354
MboI GATC 3 cut(s) 133, 252, 346
MboII GAAGA 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 2 cut(s) 469, 499
MlyI GAGTC 4 cut(s) 20, 122, 428, 605
MmeI TCCRAC 2 cut(s) 204, 317
MroI TCCGGA 2 cut(s) 257, 599
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 8 cut(s) 247, 258, 361, 416, 548, 600, 617, 795
MspR9I CCNGG 6 cut(s) 223, 322, 361, 549, 618, 795
MvaI CCWGG 2 cut(s) 223, 322
MvnI CGCG 2 cut(s) 315, 635
MwoI GCNNNNNNNGC 6 cut(s) 91, 271, 351, 389, 473, 738
NciI CCSGG 4 cut(s) 361, 549, 618, 795
NdeII GATC 3 cut(s) 133, 252, 346
NlaIII CATG 2 cut(s) 174, 220
NlaIV GGNNCC 5 cut(s) 35, 266, 319, 358, 359
NmeAIII GCCGAG 1 cut(s) 591
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 436
PciI ACATGT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 3 cut(s) 44, 288, 544
PkrI GCNGC 5 cut(s) 309, 312, 344, 371, 391
Ple19I CGATCG 1 cut(s) 349
PleI GAGTC 4 cut(s) 20, 122, 428, 604
PpsI GAGTC 4 cut(s) 20, 122, 428, 604
PpuMI RGGWCCY 1 cut(s) 357
PscI ACATGT 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 221, 320
PspEI GGTNACC 1 cut(s) 620
PspGI CCWGG 2 cut(s) 221, 320
PspN4I GGNNCC 5 cut(s) 35, 266, 319, 358, 359
PspPI GGNCC 4 cut(s) 318, 357, 474, 563
PspPPI RGGWCCY 1 cut(s) 357
PspXI VCTCGAGB 1 cut(s) 436
PvuI CGATCG 1 cut(s) 349
RsaI GTAC 3 cut(s) 52, 430, 715
RsaNI GTAC 3 cut(s) 51, 429, 714
RseI CAYNNNNRTG 1 cut(s) 215
SalI GTCGAC 1 cut(s) 236
SatI GCNGC 5 cut(s) 308, 311, 343, 370, 390
Sau3AI GATC 3 cut(s) 133, 252, 346
Sau96I GGNCC 4 cut(s) 318, 357, 474, 563
SchI GAGTC 4 cut(s) 20, 122, 428, 605
ScrFI CCNGG 6 cut(s) 223, 322, 361, 549, 618, 795
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 3 cut(s) 454, 571, 667
SfcI CTRYAG 1 cut(s) 806
Sfr274I CTCGAG 1 cut(s) 436
SinI GGWCC 2 cut(s) 318, 357
SlaI CTCGAG 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 2 cut(s) 469, 499
SsiI CCGC 6 cut(s) 105, 307, 310, 315, 354, 635
SspMI CTAG 2 cut(s) 125, 495
StyD4I CCNGG 6 cut(s) 221, 320, 359, 547, 616, 793
TaaI ACNGT 1 cut(s) 626
TaiI ACGT 3 cut(s) 10, 194, 404
TaqI TCGA 7 cut(s) 24, 204, 213, 237, 437, 503, 737
TasI AATT 2 cut(s) 469, 499
TatI WGTACW 1 cut(s) 50
TauI GCSGC 2 cut(s) 310, 313
TfiI GAWTC 3 cut(s) 44, 288, 544
TscAI CASTG 1 cut(s) 748
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 342, 369, 389
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 280, 669
TspGWI ACGGA 2 cut(s) 364, 446
TspRI CASTG 1 cut(s) 748
VpaK11BI GGWCC 2 cut(s) 318, 357
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 2 cut(s) 125, 495
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.