Prupe.8G261000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009253
Physical Location & Seq
Forward (+)
22055341 .. 22057371
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G261000.1

Sequence Viewer

Length: 849 bp
ATGTCACACAAAGCCCTAGAACACCGGCACCCCATCGATTCGTGCGCATTTCAGCTCCATAGCTGGAGACCTTTTCATCTGCATCAACAGACCACACCTACCTCTAAAACCCTAGACTCCGATCCCTCCCTCCCCAACCCCAAACCCTACAACTCCTCGTCCAATGGCCTGGTGGTCCACACCAAGCGTCCTTGCCTATCCAACCGAGCGACCTCGTTTTCAATCGACGCCATCGACATGTCTCGGTTGACCTTGGTCGACGACGACAGGACGATCTCCGGTGGCCATCACAATAGGCACGGCAGCTTTCGATTCATTGCGAAGAAGAGGCGGCGCCACGGGTCCAGGTCAGTTTCGGGTCGGAGCAGCGATCGGAGTGGGACCCGGAGGTGCTGCTCGGTCGGGGCTTCGGCTGCGTACGGTACGTGCTCGGATTTTCCGGTGGCGGTGGGTACGGACTCCAGTGGTGAGCTGTTTGGGAATGGGGATGCAAATTGGGCATCGGATGTGAGTGAAGCTAGGAATTCGAGGAAGGAGAGAGATGGAGGTGGGAGTGGAGAGAAGGAGAATCTGGGTATTGGGTTTGGGCCCATTGGGGGTTTTGATGTTCAGGGGAATGAGTCCGGGTACGGCAGCGAACCGGGTTACCGAGGGGATGCGGAGTTCGGGTACGGCGATGAGCTTGATGAGGAGGAGGAGGATACTCGTCTGTTGTTTTGGGGTGATCAATTTGGAGATGCTGATTCCATGATGGAGATTGTGGGGGAAAATACATTTGTCGATCAAAAATCTCATCACAGATGTCGGCGTAAGAAGCACGACTGCAGAATGGTTGATACGCTGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.1

Weight (kDa)

6.39

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 46
AccB1I GGYRCC 2 cut(s) 27, 333
AccI GTMKAC 1 cut(s) 258
AciI CCGC 3 cut(s) 331, 446, 659
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 523
AcyI GRCGYC 2 cut(s) 228, 334
AfaI GTAC 5 cut(s) 419, 424, 454, 629, 671
AfiI CCNNNNNNNGG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 1 cut(s) 222
AjnI CCWGG 2 cut(s) 168, 344
AloI GAACNNNNNNTCC 2 cut(s) 647, 679
AluBI AGCT 6 cut(s) 55, 63, 306, 472, 518, 682
AluI AGCT 6 cut(s) 55, 63, 306, 472, 518, 682
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 2 cut(s) 61, 246
AlwI GGATC 1 cut(s) 116
AoxI GGCC 3 cut(s) 166, 283, 587
ApaI GGGCCC 1 cut(s) 591
ApeKI GCWGC 5 cut(s) 303, 366, 393, 413, 633
ApoI RAATTY 1 cut(s) 523
AspLEI GCGC 2 cut(s) 47, 336
AspS9I GGNCC 5 cut(s) 175, 342, 381, 587, 588
AsuC2I CCSGG 3 cut(s) 385, 625, 642
AsuHPI GGTGA 2 cut(s) 479, 734
AvaII GGWCC 3 cut(s) 175, 342, 381
BaeGI GKGCMC 1 cut(s) 591
BalI TGGCCA 1 cut(s) 285
BanI GGYRCC 2 cut(s) 27, 333
BanII GRGCYC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 431
BbvCI CCTCAGC 1 cut(s) 842
BbvI GCAGC 5 cut(s) 315, 378, 380, 400, 645
BccI CCATC 5 cut(s) 41, 239, 294, 536, 745
BceAI ACGGC 3 cut(s) 316, 646, 688
BciT130I CCWGG 2 cut(s) 170, 346
BciVI GTATCC 1 cut(s) 694
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 3 cut(s) 385, 625, 642
BcoDI GTCTC 2 cut(s) 61, 246
BfaI CTAG 3 cut(s) 17, 113, 519
BfmI CTRYAG 1 cut(s) 823
BfoI RGCGCY 1 cut(s) 337
BfuI GTATCC 1 cut(s) 694
BisI GCNGC 6 cut(s) 304, 332, 367, 394, 414, 634
BlsI GCNGC 6 cut(s) 305, 333, 368, 395, 415, 635
Bme1390I CCNGG 5 cut(s) 170, 346, 385, 625, 642
Bme18I GGWCC 3 cut(s) 175, 342, 381
BmgT120I GGNCC 5 cut(s) 175, 342, 381, 587, 588
BmiI GGNNCC 6 cut(s) 29, 335, 343, 382, 383, 589
BmrFI CCNGG 5 cut(s) 170, 346, 385, 625, 642
BmsI GCATC 5 cut(s) 91, 478, 509, 646, 727
BoxI GACNNNNGTC 1 cut(s) 254
BpmI CTGGAG 2 cut(s) 85, 445
Bpu10I CCTNAGC 1 cut(s) 842
BpuMI CCSGG 3 cut(s) 385, 625, 642
Bsa29I ATCGAT 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 426
BsaHI GRCGYC 2 cut(s) 228, 334
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 252, 337, 649
BsaWI WCCGGW 2 cut(s) 278, 439
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 1 cut(s) 462
Bse3DI GCAATG 1 cut(s) 315
BseBI CCWGG 2 cut(s) 170, 346
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 252, 337, 649
BseGI GGATG 3 cut(s) 493, 511, 661
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 1 cut(s) 462
BseRI GAGGAG 4 cut(s) 145, 704, 707, 710
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 5 cut(s) 315, 378, 380, 400, 645
Bsh1285I CGRYCG 2 cut(s) 373, 402
BshFI GGCC 3 cut(s) 168, 285, 589
BshNI GGYRCC 2 cut(s) 27, 333
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 2 cut(s) 373, 402
BsiHKAI GWGCWC 1 cut(s) 431
BsiSI CCGG 6 cut(s) 25, 279, 385, 440, 624, 641
BsiWI CGTACG 1 cut(s) 417
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 2 cut(s) 61, 246
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 3 cut(s) 168, 285, 589
Bso31I GGTCTC 1 cut(s) 61
Bsp120I GGGCCC 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 431, 591
Bsp143I GATC 5 cut(s) 121, 273, 370, 724, 781
BspACI CCGC 3 cut(s) 331, 446, 659
BspANI GGCC 3 cut(s) 168, 285, 589
BspCNI CTCAG 1 cut(s) 834
BspDI ATCGAT 1 cut(s) 36
BspLI GGNNCC 6 cut(s) 29, 335, 343, 382, 383, 589
BspMAI CTGCAG 1 cut(s) 827
BspPI GGATC 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 27, 333
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 315
BsrFI RCCGGY 1 cut(s) 24
BsrI ACTGG 1 cut(s) 462
BssAI RCCGGY 1 cut(s) 24
BssECI CCNNGG 3 cut(s) 252, 337, 649
BssMI GATC 5 cut(s) 121, 273, 370, 724, 781
BssNI GRCGYC 2 cut(s) 228, 334
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 2 cut(s) 170, 346
Bst4CI ACNGT 1 cut(s) 422
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 2 cut(s) 228, 334
BstBAI YACGTR 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 842
BstDSI CCRYGG 1 cut(s) 337
BstEII GGTNACC 1 cut(s) 644
BstF5I GGATG 3 cut(s) 493, 511, 661
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 2 cut(s) 47, 336
BstKTI GATC 5 cut(s) 124, 276, 373, 727, 784
BstMAI GTCTC 2 cut(s) 61, 246
BstMBI GATC 5 cut(s) 121, 273, 370, 724, 781
BstMCI CGRYCG 2 cut(s) 373, 402
BstMWI GCNNNNNNNGC 3 cut(s) 413, 497, 814
BstNI CCWGG 2 cut(s) 170, 346
BstNSI RCATGY 1 cut(s) 241
BstPAI GACNNNNGTC 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 5 cut(s) 168, 344, 383, 623, 640
BstSFI CTRYAG 1 cut(s) 823
BstSLI GKGCMC 1 cut(s) 591
BstV1I GCAGC 5 cut(s) 315, 378, 380, 400, 645
BstXI CCANNNNNNTGG 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 36
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 3 cut(s) 168, 285, 589
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 690
BtsCI GGATG 3 cut(s) 493, 511, 661
BtsIMutI CAGTG 1 cut(s) 469
CfoI GCGC 2 cut(s) 47, 336
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 5 cut(s) 175, 342, 381, 587, 588
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 2 cut(s) 176, 236
Csp6I GTAC 5 cut(s) 418, 423, 453, 628, 670
CviAII CATG 2 cut(s) 238, 748
CviQI GTAC 5 cut(s) 418, 423, 453, 628, 670
DdeI CTNAG 1 cut(s) 842
DinI GGCGCC 1 cut(s) 335
DpnI GATC 5 cut(s) 123, 275, 372, 726, 783
DpnII GATC 5 cut(s) 121, 273, 370, 724, 781
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 3 cut(s) 175, 342, 381
Eco91I GGTNACC 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 381
EcoO65I GGTNACC 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 168, 344
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 335
EheI GGCGCC 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 241, 751
FaiI YATR 3 cut(s) 60, 239, 749
FaqI GGGAC 1 cut(s) 394
FatI CATG 2 cut(s) 237, 747
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 6 cut(s) 304, 332, 367, 394, 414, 634
FokI GGATG 3 cut(s) 500, 518, 668
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 6 cut(s) 304, 332, 367, 394, 414, 634
FspAI RTGCGCAY 1 cut(s) 46
FspBI CTAG 3 cut(s) 17, 113, 519
FspI TGCGCA 1 cut(s) 46
GlaI GCGC 2 cut(s) 46, 335
GluI GCNGC 6 cut(s) 304, 332, 367, 394, 414, 634
GsuI CTGGAG 2 cut(s) 85, 445
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 3 cut(s) 168, 285, 589
HapII CCGG 6 cut(s) 25, 279, 385, 440, 624, 641
HgaI GACGC 2 cut(s) 176, 236
HhaI GCGC 2 cut(s) 47, 336
Hin1I GRCGYC 2 cut(s) 228, 334
Hin1II CATG 2 cut(s) 241, 751
Hin6I GCGC 2 cut(s) 45, 334
HinP1I GCGC 2 cut(s) 45, 334
HincII GTYRAC 2 cut(s) 249, 259
HindII GTYRAC 2 cut(s) 249, 259
HinfI GANTC 7 cut(s) 38, 116, 312, 458, 568, 620, 743
HpaII CCGG 6 cut(s) 25, 279, 385, 440, 624, 641
HphI GGTGA 2 cut(s) 479, 734
Hpy166II GTNNAC 3 cut(s) 178, 249, 259
Hpy188I TCNGA 5 cut(s) 121, 363, 375, 433, 505
Hpy8I GTNNAC 3 cut(s) 178, 249, 259
Hpy99I CGWCG 3 cut(s) 230, 263, 266
HpyAV CCTTC 2 cut(s) 526, 556
HpyCH4III ACNGT 1 cut(s) 422
HpyCH4IV ACGT 1 cut(s) 425
HpyCH4V TGCA 3 cut(s) 82, 491, 825
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 497, 814
HpyF3I CTNAG 1 cut(s) 842
HpySE526I ACGT 1 cut(s) 425
Hsp92I GRCGYC 2 cut(s) 228, 334
Hsp92II CATG 2 cut(s) 241, 751
HspAI GCGC 2 cut(s) 45, 334
KasI GGCGCC 1 cut(s) 333
KflI GGGWCCC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 5 cut(s) 121, 273, 370, 724, 781
LmnI GCTCC 2 cut(s) 60, 363
Lsp1109I GCAGC 5 cut(s) 315, 378, 380, 400, 645
LweI GCATC 5 cut(s) 91, 478, 509, 646, 727
MaeI CTAG 3 cut(s) 17, 113, 519
MaeII ACGT 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 3, 644
MalI GATC 5 cut(s) 123, 275, 372, 726, 783
MboI GATC 5 cut(s) 121, 273, 370, 724, 781
MboII GAAGA 2 cut(s) 334, 337
MhlI GDGCHC 2 cut(s) 431, 591
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 3 cut(s) 493, 523, 728
MluNI TGGCCA 1 cut(s) 285
Mly113I GGCGCC 1 cut(s) 334
MlyI GAGTC 3 cut(s) 110, 452, 629
MmeI TCCRAC 2 cut(s) 225, 341
Mox20I TGGCCA 1 cut(s) 285
MscI TGGCCA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 236
Msp20I TGGCCA 1 cut(s) 285
MspI CCGG 6 cut(s) 25, 279, 385, 440, 624, 641
MspR9I CCNGG 5 cut(s) 170, 346, 385, 625, 642
MvaI CCWGG 2 cut(s) 170, 346
MwoI GCNNNNNNNGC 3 cut(s) 413, 497, 814
NarI GGCGCC 1 cut(s) 334
NciI CCSGG 3 cut(s) 385, 625, 642
NdeII GATC 5 cut(s) 121, 273, 370, 724, 781
NlaIII CATG 2 cut(s) 241, 751
NlaIV GGNNCC 6 cut(s) 29, 335, 343, 382, 383, 589
NmuCI GTSAC 1 cut(s) 3
NsbI TGCGCA 1 cut(s) 46
NspI RCATGY 1 cut(s) 241
PciI ACATGT 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 672
PfeI GAWTC 4 cut(s) 38, 312, 568, 743
Pfl23II CGTACG 1 cut(s) 417
PkrI GCNGC 6 cut(s) 305, 333, 368, 395, 415, 635
Ple19I CGATCG 1 cut(s) 373
PleI GAGTC 3 cut(s) 110, 452, 628
PluTI GGCGCC 1 cut(s) 337
PpsI GAGTC 3 cut(s) 110, 452, 628
Ppu21I YACGTR 1 cut(s) 426
PpuMI RGGWCCY 1 cut(s) 381
PscI ACATGT 1 cut(s) 237
PshAI GACNNNNGTC 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 168, 344
PspEI GGTNACC 1 cut(s) 644
PspGI CCWGG 2 cut(s) 168, 344
PspLI CGTACG 1 cut(s) 417
PspN4I GGNNCC 6 cut(s) 29, 335, 343, 382, 383, 589
PspOMI GGGCCC 1 cut(s) 587
PspPI GGNCC 5 cut(s) 175, 342, 381, 587, 588
PspPPI RGGWCCY 1 cut(s) 381
PstI CTGCAG 1 cut(s) 827
PvuI CGATCG 1 cut(s) 373
RsaI GTAC 5 cut(s) 419, 424, 454, 629, 671
RsaNI GTAC 5 cut(s) 418, 423, 453, 628, 670
RseI CAYNNNNRTG 1 cut(s) 236
SalI GTCGAC 1 cut(s) 257
SatI GCNGC 6 cut(s) 304, 332, 367, 394, 414, 634
Sau3AI GATC 5 cut(s) 121, 273, 370, 724, 781
Sau96I GGNCC 5 cut(s) 175, 342, 381, 587, 588
SchI GAGTC 3 cut(s) 110, 452, 629
ScrFI CCNGG 5 cut(s) 170, 346, 385, 625, 642
SduI GDGCHC 2 cut(s) 431, 591
SfaNI GCATC 5 cut(s) 91, 478, 509, 646, 727
SfcI CTRYAG 1 cut(s) 823
SfoI GGCGCC 1 cut(s) 335
SinI GGWCC 3 cut(s) 175, 342, 381
SmiMI CAYNNNNRTG 1 cut(s) 236
Sse9I AATT 3 cut(s) 493, 523, 728
SsiI CCGC 3 cut(s) 331, 446, 659
SspDI GGCGCC 1 cut(s) 333
SspMI CTAG 3 cut(s) 17, 113, 519
StyD4I CCNGG 5 cut(s) 168, 344, 383, 623, 640
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 422
TaiI ACGT 1 cut(s) 428
TaqI TCGA 7 cut(s) 36, 225, 234, 258, 310, 527, 780
TaqII GACCGA 1 cut(s) 388
TasI AATT 3 cut(s) 493, 523, 728
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 4 cut(s) 38, 312, 568, 743
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 5 cut(s) 303, 366, 393, 413, 633
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 65, 304
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 3 cut(s) 175, 342, 381
XapI RAATTY 1 cut(s) 523
XceI RCATGY 1 cut(s) 241
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 3 cut(s) 17, 113, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.