pycom11g27120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009253
Physical Location & Seq
Reverse (-)
29489158 .. 29490876
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g27120.1

Sequence Viewer

Length: 819 bp
ATGTCACACAAATCCCTAGATTCACGGCGCACTTTCCAGCTCCATGGATGGAGACCTTTCCACCTGCAGCAGCAGCAGCAGACCTCCACGCCCACCTCCAAAACCCTAGACTCCTCCGATCCCAAATCCAATGGCCTCCTCGTCCACACCAAGCGTCCCTGTTTATCCAATCGAACGACTTCATTTTCTGTCGACGCCATCGACATGTCCCGGCTGACCCTGGTCGACGACGACAGGACGATCTCCGGAGGACACCATAATGGGACCGGGAGCTTTAGGTTCATCGCTAAGAAGCGGCGGCGTCGCGGGTCGAGGTCGGTTTCGGGGAGGAGTAGTGATCGGAGTGGGACCCGTAGGTGCTGCTCGGTTGGGGCTTCGGCTGCGTACGGGACGTGTTCAGATTTTCCGGTGGCAGTGGGGACGGACTCGAGTGGGGAGCTGTTTGGGAATGGGGATGCGAATTGGGCGTCGGATGTGAGCGAGGCGAGGAATTCGAGGAAGGAGAGAGATGGAGGTGGGGGGAGTGGAGAGAAGGAGAATGTGGGTGCTGGGTTTGGTCCCGTTGGGGGTTTTGATATACAGGGGAATGAGTCCGGGTACGGCAGTGAACCGGGTTATCGCGGGGACGCGGAGTTCGGGTATGGCGATGAGTTTGATGAGGAGGAGGAGGATACTCGGGTATTGTTTTGGGGCGATCAGTTTGGAGATGCTGATTCTCCGATGGAGACAGTGGGGGAGAATACATTTGTGGATCAAAAGTCCCATCACAGATGTCGGCGGAAGAAACATGATTGCAGAATGGTTGATGCATTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

29.78

Weight (kDa)

6.51

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 72
AccI GTMKAC 2 cut(s) 192, 225
AccII CGCG 3 cut(s) 306, 621, 629
AccIII TCCGGA 1 cut(s) 245
AciI CCGC 6 cut(s) 295, 298, 306, 621, 629, 778
AclWI GGATC 2 cut(s) 113, 759
AcsI RAATTY 1 cut(s) 490
AcyI GRCGYC 3 cut(s) 195, 301, 467
AfaI GTAC 2 cut(s) 386, 599
AfiI CCNNNNNNNGG 1 cut(s) 566
AflIII ACRYGT 2 cut(s) 204, 392
AjiI CACGTC 1 cut(s) 393
AjnI CCWGG 1 cut(s) 219
AloI GAACNNNNNNTCC 2 cut(s) 617, 649
AluBI AGCT 3 cut(s) 40, 273, 439
AluI AGCT 3 cut(s) 40, 273, 439
Alw26I GTCTC 2 cut(s) 46, 719
AlwI GGATC 2 cut(s) 113, 759
Ama87I CYCGRG 2 cut(s) 427, 675
Aor13HI TCCGGA 1 cut(s) 245
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 6 cut(s) 67, 70, 73, 76, 360, 380
ApoI RAATTY 1 cut(s) 490
ArsI GACNNNNNNTTYG 4 cut(s) 452, 484, 617, 649
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 3 cut(s) 264, 348, 557
AsuC2I CCSGG 4 cut(s) 211, 268, 595, 612
AvaI CYCGRG 2 cut(s) 427, 675
AvaII GGWCC 3 cut(s) 264, 348, 557
BaeI ACNNNNGTAYC 4 cut(s) 589, 622, 663, 696
BbvI GCAGC 6 cut(s) 79, 82, 85, 88, 347, 367
BccI CCATC 5 cut(s) 42, 206, 503, 715, 771
BceAI ACGGC 2 cut(s) 41, 616
BciT130I CCWGG 1 cut(s) 221
BciVI GTATCC 1 cut(s) 664
BcnI CCSGG 4 cut(s) 211, 268, 595, 612
BcoDI GTCTC 2 cut(s) 46, 719
BfaI CTAG 2 cut(s) 17, 107
BfmI CTRYAG 1 cut(s) 65
BfuAI ACCTGC 1 cut(s) 72
BfuI GTATCC 1 cut(s) 664
BisI GCNGC 8 cut(s) 68, 71, 74, 77, 296, 299, 361, 381
BlsI GCNGC 8 cut(s) 69, 72, 75, 78, 297, 300, 362, 382
Bme1390I CCNGG 5 cut(s) 211, 221, 268, 595, 612
Bme18I GGWCC 3 cut(s) 264, 348, 557
BmeT110I CYCGRG 2 cut(s) 427, 675
BmgBI CACGTC 1 cut(s) 393
BmgT120I GGNCC 3 cut(s) 264, 348, 557
BmiI GGNNCC 4 cut(s) 265, 349, 350, 559
BmrFI CCNGG 5 cut(s) 211, 221, 268, 595, 612
BmsI GCATC 3 cut(s) 445, 697, 796
BoxI GACNNNNGTC 1 cut(s) 221
BpuMI CCSGG 4 cut(s) 211, 268, 595, 612
BsaHI GRCGYC 3 cut(s) 195, 301, 467
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 43, 219
BsaWI WCCGGW 2 cut(s) 245, 406
BsaXI ACNNNNNCTCC 4 cut(s) 319, 349, 623, 653
Bsc4I CCNNNNNNNGG 1 cut(s) 566
BseAI TCCGGA 1 cut(s) 245
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 43, 219
BseGI GGATG 3 cut(s) 53, 460, 478
BseLI CCNNNNNNNGG 1 cut(s) 566
BseRI GAGGAG 6 cut(s) 103, 128, 343, 674, 677, 680
BseXI GCAGC 6 cut(s) 79, 82, 85, 88, 347, 367
BseYI CCCAGC 1 cut(s) 548
Bsh1236I CGCG 3 cut(s) 306, 621, 629
BshFI GGCC 1 cut(s) 135
BsiHKCI CYCGRG 2 cut(s) 427, 675
BsiSI CCGG 6 cut(s) 211, 246, 267, 407, 594, 611
BsiWI CGTACG 1 cut(s) 384
BslFI GGGAC 9 cut(s) 141, 193, 277, 361, 403, 433, 543, 638, 745
BslI CCNNNNNNNGG 1 cut(s) 566
BsmAI GTCTC 2 cut(s) 46, 719
BsmFI GGGAC 9 cut(s) 141, 193, 277, 361, 403, 433, 543, 638, 745
BsnI GGCC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 2 cut(s) 427, 675
Bsp13I TCCGGA 1 cut(s) 245
Bsp143I GATC 5 cut(s) 118, 240, 337, 694, 751
Bsp19I CCATGG 1 cut(s) 43
BspACI CCGC 6 cut(s) 295, 298, 306, 621, 629, 778
BspANI GGCC 1 cut(s) 135
BspEI TCCGGA 1 cut(s) 245
BspFNI CGCG 3 cut(s) 306, 621, 629
BspLI GGNNCC 4 cut(s) 265, 349, 350, 559
BspMAI CTGCAG 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 2 cut(s) 113, 759
BspTNI GGTCTC 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 43, 219
BssMI GATC 5 cut(s) 118, 240, 337, 694, 751
BssNI GRCGYC 3 cut(s) 195, 301, 467
BssT1I CCWWGG 1 cut(s) 43
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 730
BstACI GRCGYC 3 cut(s) 195, 301, 467
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 3 cut(s) 53, 460, 478
BstFNI CGCG 3 cut(s) 306, 621, 629
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 5 cut(s) 121, 243, 340, 697, 754
BstMAI GTCTC 2 cut(s) 46, 719
BstMBI GATC 5 cut(s) 118, 240, 337, 694, 751
BstMWI GCNNNNNNNGC 4 cut(s) 73, 76, 380, 464
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 208
BstPAI GACNNNNGTC 1 cut(s) 221
BstSCI CCNGG 5 cut(s) 209, 219, 266, 593, 610
BstSFI CTRYAG 1 cut(s) 65
BstUI CGCG 3 cut(s) 306, 621, 629
BstV1I GCAGC 6 cut(s) 79, 82, 85, 88, 347, 367
BstXI CCANNNNNNTGG 1 cut(s) 44
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 43
BtgZI GCGATG 2 cut(s) 268, 660
BtrI CACGTC 1 cut(s) 393
BtsCI GGATG 3 cut(s) 53, 460, 478
BtsI GCAGTG 2 cut(s) 420, 610
BtsIMutI CAGTG 3 cut(s) 420, 610, 735
BveI ACCTGC 1 cut(s) 72
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 264, 348, 557
CseI GACGC 5 cut(s) 143, 203, 290, 456, 635
Csp6I GTAC 2 cut(s) 385, 598
CviAII CATG 3 cut(s) 44, 205, 788
CviJI RGCY 7 cut(s) 40, 135, 214, 273, 374, 380, 439
CviKI_1 RGCY 7 cut(s) 40, 135, 214, 273, 374, 380, 439
CviQI GTAC 2 cut(s) 385, 598
DdeI CTNAG 1 cut(s) 288
DpnI GATC 5 cut(s) 120, 242, 339, 696, 753
DpnII GATC 5 cut(s) 118, 240, 337, 694, 751
EciI GGCGGA 1 cut(s) 793
Eco130I CCWWGG 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 3 cut(s) 264, 348, 557
Eco88I CYCGRG 2 cut(s) 427, 675
EcoO109I RGGNCCY 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 43
EcoT22I ATGCAT 1 cut(s) 811
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 3 cut(s) 47, 208, 791
FaiI YATR 6 cut(s) 45, 206, 258, 578, 642, 789
FaqI GGGAC 9 cut(s) 141, 193, 277, 361, 403, 433, 543, 638, 745
FatI CATG 3 cut(s) 43, 204, 787
FauI CCCGC 2 cut(s) 299, 614
FblI GTMKAC 2 cut(s) 192, 225
Fnu4HI GCNGC 8 cut(s) 68, 71, 74, 77, 296, 299, 361, 381
FokI GGATG 3 cut(s) 60, 467, 485
Fsp4HI GCNGC 8 cut(s) 68, 71, 74, 77, 296, 299, 361, 381
FspBI CTAG 2 cut(s) 17, 107
GlaI GCGC 1 cut(s) 29
GluI GCNGC 8 cut(s) 68, 71, 74, 77, 296, 299, 361, 381
GsaI CCCAGC 1 cut(s) 552
HaeIII GGCC 1 cut(s) 135
HapII CCGG 6 cut(s) 211, 246, 267, 407, 594, 611
HgaI GACGC 5 cut(s) 143, 203, 290, 456, 635
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 3 cut(s) 195, 301, 467
Hin1II CATG 3 cut(s) 47, 208, 791
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 2 cut(s) 193, 226
HindII GTYRAC 2 cut(s) 193, 226
HinfI GANTC 5 cut(s) 20, 110, 425, 590, 713
HpaII CCGG 6 cut(s) 211, 246, 267, 407, 594, 611
Hpy166II GTNNAC 4 cut(s) 145, 193, 226, 608
Hpy188I TCNGA 5 cut(s) 118, 342, 400, 472, 720
Hpy188III TCNNGA 1 cut(s) 246
Hpy8I GTNNAC 4 cut(s) 145, 193, 226, 608
Hpy99I CGWCG 5 cut(s) 197, 230, 233, 306, 472
HpyAV CCTTC 2 cut(s) 493, 526
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 3 cut(s) 67, 795, 809
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 76, 380, 464
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 392
Hsp92I GRCGYC 3 cut(s) 195, 301, 467
Hsp92II CATG 3 cut(s) 47, 208, 791
HspAI GCGC 1 cut(s) 28
KflI GGGWCCC 1 cut(s) 348
Kpn2I TCCGGA 1 cut(s) 245
Kzo9I GATC 5 cut(s) 118, 240, 337, 694, 751
LmnI GCTCC 3 cut(s) 45, 270, 436
Lsp1109I GCAGC 6 cut(s) 79, 82, 85, 88, 347, 367
LweI GCATC 3 cut(s) 445, 697, 796
MaeI CTAG 2 cut(s) 17, 107
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 1 cut(s) 3
MalI GATC 5 cut(s) 120, 242, 339, 696, 753
MboI GATC 5 cut(s) 118, 240, 337, 694, 751
MboII GAAGA 1 cut(s) 793
MluCI AATT 2 cut(s) 460, 490
MlyI GAGTC 3 cut(s) 104, 419, 599
MmeI TCCRAC 1 cut(s) 450
Mph1103I ATGCAT 1 cut(s) 811
MroI TCCGGA 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 1 cut(s) 812
MslI CAYNNNNRTG 2 cut(s) 203, 258
MspI CCGG 6 cut(s) 211, 246, 267, 407, 594, 611
MspR9I CCNGG 5 cut(s) 211, 221, 268, 595, 612
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 3 cut(s) 306, 621, 629
MwoI GCNNNNNNNGC 4 cut(s) 73, 76, 380, 464
NciI CCSGG 4 cut(s) 211, 268, 595, 612
NcoI CCATGG 1 cut(s) 43
NdeII GATC 5 cut(s) 118, 240, 337, 694, 751
NlaIII CATG 3 cut(s) 47, 208, 791
NlaIV GGNNCC 4 cut(s) 265, 349, 350, 559
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 1 cut(s) 811
NspI RCATGY 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 427
PaqCI CACCTGC 1 cut(s) 72
PciI ACATGT 1 cut(s) 204
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 642
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 2 cut(s) 20, 713
Pfl23II CGTACG 1 cut(s) 384
PkrI GCNGC 8 cut(s) 69, 72, 75, 78, 297, 300, 362, 382
PleI GAGTC 3 cut(s) 104, 419, 598
PpsI GAGTC 3 cut(s) 104, 419, 598
PpuMI RGGWCCY 1 cut(s) 348
PscI ACATGT 1 cut(s) 204
PshAI GACNNNNGTC 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 219
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 219
PspLI CGTACG 1 cut(s) 384
PspN4I GGNNCC 4 cut(s) 265, 349, 350, 559
PspPI GGNCC 3 cut(s) 264, 348, 557
PspPPI RGGWCCY 1 cut(s) 348
PspXI VCTCGAGB 1 cut(s) 427
PstI CTGCAG 1 cut(s) 69
RsaI GTAC 2 cut(s) 386, 599
RsaNI GTAC 2 cut(s) 385, 598
RseI CAYNNNNRTG 2 cut(s) 203, 258
SalI GTCGAC 2 cut(s) 191, 224
SaqAI TTAA 1 cut(s) 812
SatI GCNGC 8 cut(s) 68, 71, 74, 77, 296, 299, 361, 381
Sau3AI GATC 5 cut(s) 118, 240, 337, 694, 751
Sau96I GGNCC 3 cut(s) 264, 348, 557
SchI GAGTC 3 cut(s) 104, 419, 599
ScrFI CCNGG 5 cut(s) 211, 221, 268, 595, 612
SfaNI GCATC 3 cut(s) 445, 697, 796
SfcI CTRYAG 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 427
SinI GGWCC 3 cut(s) 264, 348, 557
SlaI CTCGAG 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 203, 258
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 2 cut(s) 460, 490
SsiI CCGC 6 cut(s) 295, 298, 306, 621, 629, 778
SspMI CTAG 2 cut(s) 17, 107
StyD4I CCNGG 5 cut(s) 209, 219, 266, 593, 610
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 1 cut(s) 395
TaqI TCGA 7 cut(s) 172, 192, 201, 225, 311, 428, 494
TasI AATT 2 cut(s) 460, 490
TauI GCSGC 2 cut(s) 298, 301
TfiI GAWTC 2 cut(s) 20, 713
Tru1I TTAA 1 cut(s) 812
Tru9I TTAA 1 cut(s) 812
TscAI CASTG 3 cut(s) 420, 610, 735
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 6 cut(s) 67, 70, 73, 76, 360, 380
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 171, 271
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 3 cut(s) 420, 610, 735
VpaK11BI GGWCC 3 cut(s) 264, 348, 557
XapI RAATTY 1 cut(s) 490
XceI RCATGY 1 cut(s) 208
XhoI CTCGAG 1 cut(s) 427
XmiI GTMKAC 2 cut(s) 192, 225
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 2 cut(s) 17, 107
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.