Rmu_sc0036888.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0036888.1
OG0009253
Physical Location & Seq
Reverse (-)
2 .. 324
323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0036888.1_g000001.1.cds

Sequence Viewer

Length: 323 bp
atgtcacgttcccacaaagccctcgactcacggcaccccttagattcgtgtacatttcaactccatagctggagacctttccagcttcaacagcagaccaccgcacccacctccaaaaccctagactccgatcccaccaaccccaaaccctactacaccaagcgtccatgcctctccaaccgagcgacgtcgttttccatcgacgccatcgacatgtccaggctgactttggtcgacgacgaccggacgatctccggagggcaccataagcacgggagcttccgattcattgccaagaagaggcggcggcgcgggtccaggtc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

108

Amino Acids

12.43

Weight (kDa)

10.68

Isoelectric Point (pI)

83.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 191
AccB1I GGYRCC 2 cut(s) 33, 261
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 312
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 4 cut(s) 102, 304, 307, 312
AclWI GGATC 1 cut(s) 125
AcyI GRCGYC 2 cut(s) 188, 204
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 1 cut(s) 300
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 2 cut(s) 59, 89
AjnI CCWGG 2 cut(s) 218, 317
AluBI AGCT 3 cut(s) 69, 85, 279
AluI AGCT 3 cut(s) 69, 85, 279
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 1 cut(s) 125
Aor13HI TCCGGA 1 cut(s) 254
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 1 cut(s) 315
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 33, 261
BccI CCATC 2 cut(s) 206, 215
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 2 cut(s) 220, 319
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 1 cut(s) 122
BisI GCNGC 2 cut(s) 305, 308
BlsI GCNGC 2 cut(s) 306, 309
Bme1390I CCNGG 2 cut(s) 220, 319
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 3 cut(s) 35, 263, 316
BmrFI CCNGG 2 cut(s) 220, 319
BoxI GACNNNNGTC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 91
BsaHI GRCGYC 2 cut(s) 188, 204
BsaI GGTCTC 1 cut(s) 67
BsaWI WCCGGW 2 cut(s) 243, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 288
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 2 cut(s) 220, 319
BseLI CCNNNNNNNGG 1 cut(s) 300
BseMI GCAATG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 244
BshNI GGYRCC 2 cut(s) 33, 261
BsiEI CGRYCG 1 cut(s) 244
BsiSI CCGG 2 cut(s) 244, 255
BslI CCNNNNNNNGG 1 cut(s) 300
BsmAI GTCTC 1 cut(s) 67
Bso31I GGTCTC 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 50
Bsp143I GATC 2 cut(s) 130, 249
BspACI CCGC 4 cut(s) 102, 304, 307, 312
BspEI TCCGGA 1 cut(s) 254
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 3 cut(s) 35, 263, 316
BspPI GGATC 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 33, 261
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 288
BsrGI TGTACA 1 cut(s) 50
BssMI GATC 2 cut(s) 130, 249
BssNI GRCGYC 2 cut(s) 188, 204
Bst2UI CCWGG 2 cut(s) 220, 319
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 2 cut(s) 188, 204
BstAUI TGTACA 1 cut(s) 50
BstDEI CTNAG 1 cut(s) 40
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 312
BstKTI GATC 2 cut(s) 133, 252
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 2 cut(s) 130, 249
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 91, 268
BstNI CCWGG 2 cut(s) 220, 319
BstNSI RCATGY 1 cut(s) 217
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 218, 317
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 1 cut(s) 312
CfoI GCGC 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 2 cut(s) 152, 212
Csp6I GTAC 1 cut(s) 51
CviAII CATG 2 cut(s) 168, 214
CviJI RGCY 5 cut(s) 20, 69, 85, 223, 279
CviKI_1 RGCY 5 cut(s) 20, 69, 85, 223, 279
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 40
DpnI GATC 2 cut(s) 132, 251
DpnII GATC 2 cut(s) 130, 249
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 315
EcoRII CCWGG 2 cut(s) 218, 317
FaeI CATG 2 cut(s) 171, 217
FaiI YATR 4 cut(s) 66, 169, 215, 267
FatI CATG 2 cut(s) 167, 213
FauI CCCGC 1 cut(s) 305
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 2 cut(s) 305, 308
Fsp4HI GCNGC 2 cut(s) 305, 308
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 311
GluI GCNGC 2 cut(s) 305, 308
GsuI CTGGAG 1 cut(s) 91
HapII CCGG 2 cut(s) 244, 255
HgaI GACGC 2 cut(s) 152, 212
HhaI GCGC 1 cut(s) 312
Hin1I GRCGYC 2 cut(s) 188, 204
Hin1II CATG 2 cut(s) 171, 217
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 4 cut(s) 26, 44, 125, 285
HpaII CCGG 2 cut(s) 244, 255
Hpy166II GTNNAC 2 cut(s) 51, 235
Hpy188I TCNGA 2 cut(s) 130, 284
Hpy188III TCNNGA 1 cut(s) 255
Hpy8I GTNNAC 2 cut(s) 51, 235
Hpy99I CGWCG 5 cut(s) 190, 193, 206, 239, 242
HpyCH4IV ACGT 2 cut(s) 7, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 268
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 7, 188
Hsp92I GRCGYC 2 cut(s) 188, 204
Hsp92II CATG 2 cut(s) 171, 217
HspAI GCGC 1 cut(s) 310
Kpn2I TCCGGA 1 cut(s) 254
Kzo9I GATC 2 cut(s) 130, 249
LmnI GCTCC 1 cut(s) 276
LpnPI CCDG 7 cut(s) 55, 95, 205, 232, 257, 268, 304
MaeI CTAG 1 cut(s) 122
MaeII ACGT 2 cut(s) 7, 188
MaeIII GTNAC 1 cut(s) 3
MalI GATC 2 cut(s) 132, 251
MboI GATC 2 cut(s) 130, 249
MboII GAAGA 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 264
MlyI GAGTC 2 cut(s) 20, 119
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 5 cut(s) 32, 121, 182, 251, 294
MroI TCCGGA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 212
MspI CCGG 2 cut(s) 244, 255
MspR9I CCNGG 2 cut(s) 220, 319
MvaI CCWGG 2 cut(s) 220, 319
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 91, 268
NdeII GATC 2 cut(s) 130, 249
NlaIII CATG 2 cut(s) 171, 217
NlaIV GGNNCC 3 cut(s) 35, 263, 316
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 217
PciI ACATGT 1 cut(s) 213
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 2 cut(s) 44, 285
PkrI GCNGC 2 cut(s) 306, 309
PleI GAGTC 2 cut(s) 20, 119
PpsI GAGTC 2 cut(s) 20, 119
PscI ACATGT 1 cut(s) 213
PshAI GACNNNNGTC 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 218, 317
PspGI CCWGG 2 cut(s) 218, 317
PspN4I GGNNCC 3 cut(s) 35, 263, 316
PspPI GGNCC 1 cut(s) 315
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 212
SalI GTCGAC 1 cut(s) 233
SatI GCNGC 2 cut(s) 305, 308
Sau3AI GATC 2 cut(s) 130, 249
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 2 cut(s) 20, 119
ScrFI CCNGG 2 cut(s) 220, 319
SduI GDGCHC 1 cut(s) 264
SetI ASST 8 cut(s) 10, 71, 79, 87, 113, 191, 281, 323
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 212
SsiI CCGC 4 cut(s) 102, 304, 307, 312
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 218, 317
TaiI ACGT 2 cut(s) 10, 191
TaqI TCGA 4 cut(s) 24, 201, 210, 234
TatI WGTACW 1 cut(s) 50
TauI GCSGC 2 cut(s) 307, 310
TfiI GAWTC 2 cut(s) 44, 285
TseFI GTSAC 1 cut(s) 3
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 315
XceI RCATGY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 1 cut(s) 122
ZraI GACGTC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.