AT4G04480

F-box protein At4g22030

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009170
Physical Location & Seq
Forward (+)
2229649 .. 2230851
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G04480.1

Sequence Viewer

Length: 1197 bp
ATGGCTTCATTGCAGGTATCAAGATTAGTTCTTTCGTCATCTTCTTCTAGTACAAGACCTAAAGCAGCAGTTTCAATCCCGAAGCTACCGAAATTTCACGTCTCGGTTCCGAAAATACCGACTTTATCGGTTAAAGAACCGAGCTTTGAGTTTGTAGAAAGTCTTCCTCTTTCGTTGAAACAAAAGAGAGGTTCTGATTCTATCCAAAGAACTCGTCTCAATGCGGTTCTTGAAGAAGTTATGGATAGGATTGAGATGCACAAGAACATCGGAGATCAACGTAACAATTGGAACTCTTTACTGCTCAATTCTGTCAACATGATTACTCTCACTGCCGCTTTGATGGCCGGAATAGCCTCGGTTAACGGTCACGGTGTAGATTCTGTAACCGCTGTGAAGATAGCTTCAACGGTGTTGTTAACATCTGCGACGGGTTTTGCTGCCTTGATGAGCAAGATTCAACCTTCGCAGCTAGCTGAAGAACAGAGGAACGCGACGAGGCTGTTCAAGAAGCTGAGAGTCGAAATCGAAATGTTTCTGAGAGAAAACGAGGAGATCGAAGAAGAAGATGTTAAGGAAGTGATAAAGAGAGTGTTGTCTCTAGATAAAGCTTATCCTCTTCCTCTGATCGGAACAATGCTCGAGAAGTTTCCGGAAGAGTTTAAACCGGCGACTTGGTGGCCTGAAAACAAGGAGAAAAACCCTAAAACTAGTCTTGCGAATGGATGGAGTCAAGAGCTTGAGGCGGAGATGAGAGAAGTTGCTCATGTGGTGAAGAGCAGAGACGCAGAAGAGTATGAGAAATTAGGTCACGTGGCGTTGAAGCTAAACCGGTTCTTGGCTATCTCTGGACCAGTTTTAACCGGAGTTTCTGCTGTAAGCTCTGTCTTTATCGGTCAAGATTCCGGTTTAGCTGGAATCGTGGCGATGACTTGTGCTTCTTTGGCTGCGGTTGTCAACACTTTAGAGCATGGTGGTCAAGTGGGAATGGTGTTTGAGATGTATAGAAACTCAGCTGGATTCTTCTCTCTATTAGAAGAAACTATGAAATCGACGGAGAAGAGAGAGAACGGACAAGTGTTTGAGACAAGAGTTGCGGTGAAGCTAGGGAGAAGCTTATCAGAACTGAGAGATCTCGCGAAAAGATCGAATCTCTCTCAGGTTAAGGCCAAAGAATTCGCTAGCAAGCTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

398

Amino Acids

43.96

Weight (kDa)

8.92

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 72 - 377 4.7e-142 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4
AccII CGCG 2 cut(s) 494, 1139
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 6 cut(s) 224, 336, 390, 746, 950, 1097
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 92, 1175
AcuI CTGAAG 1 cut(s) 498
AcvI CACGTG 1 cut(s) 814
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 629, 838
AgeI ACCGGT 1 cut(s) 831
AgsI TTSAA 7 cut(s) 75, 178, 233, 408, 461, 508, 823
AhlI ACTAGT 1 cut(s) 710
AjiI CACGTC 1 cut(s) 100
Alw26I GTCTC 5 cut(s) 106, 221, 603, 777, 1079
Ama87I CYCGRG 1 cut(s) 641
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 3 cut(s) 345, 680, 1167
ApeKI GCWGC 4 cut(s) 65, 440, 469, 947
ApoI RAATTY 2 cut(s) 92, 1175
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AsiGI ACCGGT 1 cut(s) 831
Asp700I GAANNNNTTC 2 cut(s) 162, 534
AspS9I GGNCC 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 784, 1111
AsuNHI GCTAGC 2 cut(s) 472, 1181
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 1 cut(s) 851
BarI GAAGNNNNNNTAC 2 cut(s) 147, 179
BbrPI CACGTG 1 cut(s) 814
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 4 cut(s) 77, 427, 481, 934
BccI CCATC 2 cut(s) 337, 720
BcoDI GTCTC 5 cut(s) 106, 221, 603, 777, 1079
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 7 cut(s) 48, 473, 602, 711, 1106, 1182, 1195
BfuAI ACCTGC 1 cut(s) 4
BglII AGATCT 1 cut(s) 1132
BisI GCNGC 5 cut(s) 66, 336, 441, 470, 948
BlsI GCNGC 5 cut(s) 67, 337, 442, 471, 949
Bme18I GGWCC 1 cut(s) 851
BmeT110I CYCGRG 1 cut(s) 641
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 851
BmiI GGNNCC 1 cut(s) 108
BmsI GCATC 1 cut(s) 246
BmtI GCTAGC 2 cut(s) 476, 1185
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 761
BsaAI YACGTR 1 cut(s) 814
BsaJI CCNNGG 1 cut(s) 357
BsaWI WCCGGW 4 cut(s) 652, 831, 863, 905
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 838
Bse118I RCCGGY 2 cut(s) 667, 831
Bse1I ACTGG 1 cut(s) 854
Bse3DI GCAATG 1 cut(s) 8
BseAI TCCGGA 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 731
BseLI CCNNNNNNNGG 2 cut(s) 629, 838
BseMI GCAATG 1 cut(s) 8
BseMII CTCAG 5 cut(s) 506, 530, 1026, 1118, 1172
BseNI ACTGG 1 cut(s) 854
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 4 cut(s) 77, 427, 481, 934
Bsh1236I CGCG 2 cut(s) 494, 1139
BshFI GGCC 3 cut(s) 347, 682, 1169
BshTI ACCGGT 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 6 cut(s) 348, 653, 668, 832, 864, 906
BslI CCNNNNNNNGG 2 cut(s) 629, 838
BsmAI GTCTC 5 cut(s) 106, 221, 603, 777, 1079
BsmBI CGTCTC 3 cut(s) 106, 221, 777
BsnI GGCC 3 cut(s) 347, 682, 1169
BsoBI CYCGRG 1 cut(s) 641
Bsp13I TCCGGA 1 cut(s) 652
Bsp143I GATC 5 cut(s) 274, 555, 627, 1132, 1145
Bsp68I TCGCGA 1 cut(s) 1139
BspACI CCGC 6 cut(s) 224, 336, 390, 746, 950, 1097
BspANI GGCC 3 cut(s) 347, 682, 1169
BspCNI CTCAG 5 cut(s) 507, 531, 1025, 1119, 1171
BspEI TCCGGA 1 cut(s) 652
BspFNI CGCG 2 cut(s) 494, 1139
BspLI GGNNCC 1 cut(s) 108
BspMI ACCTGC 1 cut(s) 4
BspOI GCTAGC 2 cut(s) 476, 1185
BspQI GCTCTTC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 8
BsrFI RCCGGY 2 cut(s) 667, 831
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 2 cut(s) 667, 831
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 5 cut(s) 274, 555, 627, 1132, 1145
Bst4CI ACNGT 3 cut(s) 368, 374, 412
Bst6I CTCTTC 5 cut(s) 624, 651, 770, 786, 1055
BstBAI YACGTR 1 cut(s) 814
BstC8I GCNNGC 3 cut(s) 474, 1183, 1187
BstDEI CTNAG 5 cut(s) 515, 539, 1012, 1127, 1158
BstF5I GGATG 1 cut(s) 731
BstFNI CGCG 2 cut(s) 494, 1139
BstKTI GATC 5 cut(s) 277, 558, 630, 1135, 1148
BstMAI GTCTC 5 cut(s) 106, 221, 603, 777, 1079
BstMBI GATC 5 cut(s) 274, 555, 627, 1132, 1145
BstMWI GCNNNNNNNGC 3 cut(s) 344, 353, 944
BstUI CGCG 2 cut(s) 494, 1139
BstV1I GCAGC 4 cut(s) 77, 427, 481, 934
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 1132
BstYI RGATCY 1 cut(s) 1132
BsuRI GGCC 3 cut(s) 347, 682, 1169
BtgZI GCGATG 1 cut(s) 941
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 731
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
BtuMI TCGCGA 1 cut(s) 1139
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 3 cut(s) 474, 1183, 1187
Cfr10I RCCGGY 2 cut(s) 667, 831
Cfr13I GGNCC 1 cut(s) 851
CseI GACGC 1 cut(s) 794
Csp6I GTAC 1 cut(s) 51
CspAI ACCGGT 1 cut(s) 831
CviAII CATG 3 cut(s) 319, 767, 971
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 5 cut(s) 515, 539, 1012, 1127, 1158
DpnI GATC 5 cut(s) 276, 557, 629, 1134, 1147
DpnII GATC 5 cut(s) 274, 555, 627, 1132, 1145
DraI TTTAAA 1 cut(s) 664
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 5 cut(s) 624, 651, 770, 786, 1055
EarI CTCTTC 5 cut(s) 624, 651, 770, 786, 1055
EciI GGCGGA 1 cut(s) 761
Eco47I GGWCC 1 cut(s) 851
Eco57I CTGAAG 1 cut(s) 498
Eco72I CACGTG 1 cut(s) 814
Eco88I CYCGRG 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 1175
Esp3I CGTCTC 3 cut(s) 106, 221, 777
FaeI CATG 3 cut(s) 322, 770, 974
FaiI YATR 7 cut(s) 242, 320, 768, 798, 972, 1005, 1046
FatI CATG 3 cut(s) 318, 766, 970
Fnu4HI GCNGC 5 cut(s) 66, 336, 441, 470, 948
FokI GGATG 1 cut(s) 738
Fsp4HI GCNGC 5 cut(s) 66, 336, 441, 470, 948
FspBI CTAG 7 cut(s) 48, 473, 602, 711, 1106, 1182, 1195
GluI GCNGC 5 cut(s) 66, 336, 441, 470, 948
HaeIII GGCC 3 cut(s) 347, 682, 1169
HapII CCGG 6 cut(s) 348, 653, 668, 832, 864, 906
HgaI GACGC 1 cut(s) 794
Hin1II CATG 3 cut(s) 322, 770, 974
HincII GTYRAC 4 cut(s) 316, 364, 420, 958
HindII GTYRAC 4 cut(s) 316, 364, 420, 958
HindIII AAGCTT 3 cut(s) 609, 1114, 1187
HinfI GANTC 9 cut(s) 197, 380, 457, 519, 730, 902, 918, 1020, 1150
HpaI GTTAAC 2 cut(s) 364, 420
HpaII CCGG 6 cut(s) 348, 653, 668, 832, 864, 906
HphI GGTGA 2 cut(s) 784, 1111
Hpy166II GTNNAC 4 cut(s) 316, 364, 420, 958
Hpy188I TCNGA 7 cut(s) 111, 196, 272, 540, 627, 632, 1123
Hpy8I GTNNAC 4 cut(s) 316, 364, 420, 958
Hpy99I CGWCG 3 cut(s) 433, 499, 1057
HpyAV CCTTC 1 cut(s) 474
HpyCH4III ACNGT 3 cut(s) 368, 374, 412
HpyCH4IV ACGT 3 cut(s) 99, 280, 813
HpyCH4V TGCA 2 cut(s) 13, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 353, 944
HpyF3I CTNAG 5 cut(s) 515, 539, 1012, 1127, 1158
HpySE526I ACGT 3 cut(s) 99, 280, 813
Hsp92II CATG 3 cut(s) 322, 770, 974
Kpn2I TCCGGA 1 cut(s) 652
KspAI GTTAAC 2 cut(s) 364, 420
Kzo9I GATC 5 cut(s) 274, 555, 627, 1132, 1145
LguI GCTCTTC 1 cut(s) 770
Lsp1109I GCAGC 4 cut(s) 77, 427, 481, 934
LweI GCATC 1 cut(s) 246
MaeI CTAG 7 cut(s) 48, 473, 602, 711, 1106, 1182, 1195
MaeII ACGT 3 cut(s) 99, 280, 813
MaeIII GTNAC 4 cut(s) 281, 368, 385, 809
MalI GATC 5 cut(s) 276, 557, 629, 1134, 1147
MboI GATC 5 cut(s) 274, 555, 627, 1132, 1145
MfeI CAATTG 1 cut(s) 286
MflI RGATCY 1 cut(s) 1132
MluCI AATT 5 cut(s) 92, 286, 307, 803, 1175
MlyI GAGTC 2 cut(s) 528, 739
MnlI CCTC 9 cut(s) 177, 182, 367, 480, 492, 544, 627, 633, 736
MroI TCCGGA 1 cut(s) 652
MroXI GAANNNNTTC 2 cut(s) 162, 534
MseI TTAA 7 cut(s) 132, 363, 419, 573, 663, 860, 1164
MspA1I CMGCKG 2 cut(s) 392, 1016
MspI CCGG 6 cut(s) 348, 653, 668, 832, 864, 906
MssI GTTTAAAC 1 cut(s) 664
MunI CAATTG 1 cut(s) 286
MvnI CGCG 2 cut(s) 494, 1139
MwoI GCNNNNNNNGC 3 cut(s) 344, 353, 944
NdeII GATC 5 cut(s) 274, 555, 627, 1132, 1145
NheI GCTAGC 2 cut(s) 472, 1181
NlaIII CATG 3 cut(s) 322, 770, 974
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 2 cut(s) 368, 809
NruI TCGCGA 1 cut(s) 1139
PaeR7I CTCGAG 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 770
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PdmI GAANNNNTTC 2 cut(s) 162, 534
PfeI GAWTC 7 cut(s) 197, 380, 457, 902, 918, 1020, 1150
PinAI ACCGGT 1 cut(s) 831
PkrI GCNGC 5 cut(s) 67, 337, 442, 471, 949
PleI GAGTC 2 cut(s) 527, 738
PmaCI CACGTG 1 cut(s) 814
PmeI GTTTAAAC 1 cut(s) 664
PmlI CACGTG 1 cut(s) 814
PpsI GAGTC 2 cut(s) 527, 738
Ppu21I YACGTR 1 cut(s) 814
PspCI CACGTG 1 cut(s) 814
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 1 cut(s) 851
PsuI RGATCY 1 cut(s) 1132
PvuII CAGCTG 1 cut(s) 1016
RruI TCGCGA 1 cut(s) 1139
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 770
SaqAI TTAA 7 cut(s) 132, 363, 419, 573, 663, 860, 1164
SatI GCNGC 5 cut(s) 66, 336, 441, 470, 948
Sau3AI GATC 5 cut(s) 274, 555, 627, 1132, 1145
Sau96I GGNCC 1 cut(s) 851
SchI GAGTC 2 cut(s) 528, 739
SfaNI GCATC 1 cut(s) 246
Sfr274I CTCGAG 1 cut(s) 641
SinI GGWCC 1 cut(s) 851
SlaI CTCGAG 1 cut(s) 641
SmlI CTYRAG 2 cut(s) 641, 740
SmoI CTYRAG 2 cut(s) 641, 740
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 5 cut(s) 92, 286, 307, 803, 1175
SsiI CCGC 6 cut(s) 224, 336, 390, 746, 950, 1097
SspMI CTAG 7 cut(s) 48, 473, 602, 711, 1106, 1182, 1195
TaaI ACNGT 3 cut(s) 368, 374, 412
TaiI ACGT 3 cut(s) 102, 283, 816
TaqI TCGA 6 cut(s) 522, 528, 558, 642, 1052, 1148
TaqII GACCGA 1 cut(s) 884
TasI AATT 5 cut(s) 92, 286, 307, 803, 1175
TatI WGTACW 1 cut(s) 50
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 7 cut(s) 197, 380, 457, 902, 918, 1020, 1150
Tru1I TTAA 7 cut(s) 132, 363, 419, 573, 663, 860, 1164
Tru9I TTAA 7 cut(s) 132, 363, 419, 573, 663, 860, 1164
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 2 cut(s) 368, 809
TseI GCWGC 4 cut(s) 65, 440, 469, 947
Tsp45I GTSAC 2 cut(s) 368, 809
TspDTI ATGAA 1 cut(s) 1061
TspGWI ACGGA 2 cut(s) 1070, 1086
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 851
XapI RAATTY 2 cut(s) 92, 1175
XbaI TCTAGA 1 cut(s) 601
XhoI CTCGAG 1 cut(s) 641
XmnI GAANNNNTTC 2 cut(s) 162, 534
XspI CTAG 7 cut(s) 48, 473, 602, 711, 1106, 1182, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.