MD05G1349200.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009170
Physical Location & Seq
Reverse (-)
46722075 .. 46723343
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1349200.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGCTTCCCTCCAAACTTCATCTCTTCTCTTCTCTTCTCCCTCACGTTGTTCTTCCTCAAACAGAACCATAAATGCCGCCATTCATGTCCGCAAGTTCCCAAGAGTCCGCTTCGCTGACCCGAAACAAACCACGAAGCTCGTCCTCGAAGGCCTCCAGTTGAAGGAACAAGTTCTGAAACACGAAAACACCGATTTGTATAACAACAATCACTCTGCAGCAGCAGCTCCTACAGCTTCCACAACTCAGCTCTACGCGCTCTTAGAGGCGGTGGCGGACAGAGTGGAGATGCACGCAAACATCGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCACTCTCTCCGCTTCCCTAATGGCCGGAGTTAATGCAGCCGCTGGAACTTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCTACCGGAATGCTGCTTATCATGAACAAGATCCAGCCTTCGCAGCTCGCCGAGGAGCAGCGCAATGCTACGAGGTTGTTCAGGAAGCTCCAGAGCGAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGGTTTTGGCCCTTGACAAAGCTTACCCACTTCCGTTGCTCGGTGCCATGCTCGAAAAATTCCCCAAAAAATTTGAGCCGGCTGTTTGGTGGCCTAGAAATGTTGTTCAATCTCAAAATCCAAGGAAACCGAAATCCCAGATGGGAAATAATGTGAATGGATGGAGTGAAGAACTTGAAAGCGAAATGGGAGAAATTATTCAAGTTGTGAAGAACAAGGACATTGAGGACTATGAGAGATTAGGAAGCTTGGCATTAAAGACCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGCGATCGCGGCTGTTGGATCCGCTTTTGTAGGCCATGGATCAGTCATTGCCACGGTTGCAGCAGTGGCTGCAGGCTCTTTGGCTAGCACAGTGAATGCTTTTCAGCATGGCGGGCAAGTAGGGATGGTGTTTGAGATGTACAGGAACTGCGCTGGCTTCTTCAACAAGTTGGAGGATTCAATTGAAGCAACACTTGGAGAACGAGACTGGGAGAAGAGAGAAAATGGGGTGGTTTTTGAAATGAAGGTTGCAATGCAGTTGGGAAGAAGCTTGGAGCAGCTCAGAGAACTTGCATCGAAATCAGCTTTGTATCGTTCGAACGGAGAACCCATAGACGAATTCGGTTGCAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

423

Amino Acids

46.22

Weight (kDa)

7.7

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 83 - 398 1.6e-152 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 653
AccII CGCG 2 cut(s) 257, 917
AclWI GGATC 5 cut(s) 475, 569, 921, 934, 955
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 3 cut(s) 668, 680, 1247
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 2 cut(s) 163, 650
AgsI TTSAA 9 cut(s) 163, 421, 719, 788, 812, 1072, 1089, 1094, 1148
AjuI GAANNNNNNNTTGG 2 cut(s) 1086, 1118
Alw26I GTCTC 3 cut(s) 548, 593, 1107
AlwI GGATC 5 cut(s) 475, 569, 921, 934, 955
AlwNI CAGNNNCTG 3 cut(s) 398, 977, 1056
AoxI GGCC 6 cut(s) 151, 378, 618, 701, 895, 940
ApaI GGGCCC 1 cut(s) 899
ApoI RAATTY 3 cut(s) 668, 680, 1247
AsiSI GCGATCGC 1 cut(s) 915
Asp700I GAANNNNTTC 2 cut(s) 171, 807
AspLEI GCGC 4 cut(s) 259, 315, 513, 1061
AspS9I GGNCC 3 cut(s) 619, 895, 896
AsuII TTCGAA 1 cut(s) 1226
AsuNHI GCTAGC 1 cut(s) 992
BaeGI GKGCMC 1 cut(s) 899
BamHI GGATCC 1 cut(s) 926
BanI GGYRCC 1 cut(s) 653
BanII GRGCYC 1 cut(s) 899
BccI CCATC 3 cut(s) 745, 765, 1027
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 3 cut(s) 548, 593, 1107
BfaI CTAG 2 cut(s) 705, 993
BfmI CTRYAG 3 cut(s) 216, 231, 978
BmgT120I GGNCC 3 cut(s) 619, 895, 896
BmiI GGNNCC 3 cut(s) 655, 897, 928
BmrI ACTGGG 1 cut(s) 1126
BmsI GCATC 2 cut(s) 279, 1211
BmtI GCTAGC 1 cut(s) 996
BmuI ACTGGG 1 cut(s) 1126
BpmI CTGGAG 2 cut(s) 140, 524
Bpu14I TTCGAA 1 cut(s) 1226
BsaJI CCNNGG 4 cut(s) 501, 731, 943, 960
BsaWI WCCGGW 1 cut(s) 455
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 650
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 3 cut(s) 157, 326, 1121
Bse3DI GCAATG 3 cut(s) 520, 954, 1167
BseDI CCNNGG 4 cut(s) 501, 731, 943, 960
BseGI GGATG 2 cut(s) 776, 1038
BseLI CCNNNNNNNGG 2 cut(s) 163, 650
BseMI GCAATG 3 cut(s) 520, 954, 1167
BseMII CTCAG 3 cut(s) 260, 573, 1204
BseNI ACTGG 3 cut(s) 157, 326, 1121
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 899
Bsh1236I CGCG 2 cut(s) 257, 917
Bsh1285I CGRYCG 1 cut(s) 915
BshFI GGCC 6 cut(s) 153, 380, 620, 703, 897, 942
BshNI GGYRCC 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 915
BsiSI CCGG 3 cut(s) 381, 456, 689
BslI CCNNNNNNNGG 2 cut(s) 163, 650
BsmAI GTCTC 3 cut(s) 548, 593, 1107
BsmBI CGTCTC 1 cut(s) 548
BsmI GAATGC 2 cut(s) 465, 1009
BsnI GGCC 6 cut(s) 153, 380, 620, 703, 897, 942
Bsp119I TTCGAA 1 cut(s) 1226
Bsp120I GGGCCC 1 cut(s) 895
Bsp1286I GDGCHC 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 1047
Bsp143I GATC 6 cut(s) 354, 480, 574, 912, 926, 947
Bsp19I CCATGG 1 cut(s) 943
BspANI GGCC 6 cut(s) 153, 380, 620, 703, 897, 942
BspCNI CTCAG 3 cut(s) 259, 574, 1203
BspFNI CGCG 2 cut(s) 257, 917
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 655, 897, 928
BspMAI CTGCAG 2 cut(s) 220, 982
BspOI GCTAGC 1 cut(s) 996
BspPI GGATC 5 cut(s) 475, 569, 921, 934, 955
BspQI GCTCTTC 1 cut(s) 573
BspT104I TTCGAA 1 cut(s) 1226
BspT107I GGYRCC 1 cut(s) 653
BsrDI GCAATG 3 cut(s) 520, 954, 1167
BsrFI RCCGGY 1 cut(s) 688
BsrGI TGTACA 1 cut(s) 1047
BsrI ACTGG 3 cut(s) 157, 326, 1121
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 4 cut(s) 501, 731, 943, 960
BssMI GATC 6 cut(s) 354, 480, 574, 912, 926, 947
BssT1I CCWWGG 2 cut(s) 731, 943
Bst4CI ACNGT 3 cut(s) 559, 964, 1000
Bst6I CTCTTC 5 cut(s) 30, 35, 40, 573, 1118
BstAPI GCANNNNNTGC 1 cut(s) 977
BstAUI TGTACA 1 cut(s) 1047
BstBI TTCGAA 1 cut(s) 1226
BstC8I GCNNGC 9 cut(s) 294, 308, 498, 690, 982, 994, 1022, 1063, 1259
BstDEI CTNAG 5 cut(s) 246, 262, 338, 582, 1190
BstDSI CCRYGG 2 cut(s) 943, 960
BstF5I GGATG 2 cut(s) 776, 1038
BstFNI CGCG 2 cut(s) 257, 917
BstHHI GCGC 4 cut(s) 259, 315, 513, 1061
BstKTI GATC 6 cut(s) 357, 483, 577, 915, 929, 950
BstMAI GTCTC 3 cut(s) 548, 593, 1107
BstMBI GATC 6 cut(s) 354, 480, 574, 912, 926, 947
BstMCI CGRYCG 1 cut(s) 915
BstSFI CTRYAG 3 cut(s) 216, 231, 978
BstSLI GKGCMC 1 cut(s) 899
BstUI CGCG 2 cut(s) 257, 917
BstX2I RGATCY 2 cut(s) 480, 926
BstYI RGATCY 2 cut(s) 480, 926
BsuRI GGCC 6 cut(s) 153, 380, 620, 703, 897, 942
BtgI CCRYGG 2 cut(s) 943, 960
BtsCI GGATG 2 cut(s) 776, 1038
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 2 cut(s) 978, 1005
Cac8I GCNNGC 9 cut(s) 294, 308, 498, 690, 982, 994, 1022, 1063, 1259
CaiI CAGNNNCTG 3 cut(s) 398, 977, 1056
CciI TCATGA 1 cut(s) 471
CfoI GCGC 4 cut(s) 259, 315, 513, 1061
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 3 cut(s) 619, 895, 896
Csp6I GTAC 1 cut(s) 1048
CspCI CAANNNNNGTGG 2 cut(s) 627, 662
CviAII CATG 6 cut(s) 86, 352, 472, 658, 944, 1016
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 5 cut(s) 246, 262, 338, 582, 1190
DpnI GATC 6 cut(s) 356, 482, 576, 914, 928, 949
DpnII GATC 6 cut(s) 354, 480, 574, 912, 926, 947
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 5 cut(s) 30, 35, 40, 573, 1118
EarI CTCTTC 5 cut(s) 30, 35, 40, 573, 1118
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 2 cut(s) 731, 943
Eco147I AGGCCT 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 899
EcoO109I RGGNCCY 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 1247
EcoT14I CCWWGG 2 cut(s) 731, 943
EcoT38I GRGCYC 1 cut(s) 899
ErhI CCWWGG 2 cut(s) 731, 943
Esp3I CGTCTC 1 cut(s) 548
FaeI CATG 6 cut(s) 89, 355, 475, 661, 947, 1019
FalI AAGNNNNNCTT 2 cut(s) 1086, 1118
FatI CATG 6 cut(s) 85, 351, 471, 657, 943, 1015
FauI CCCGC 1 cut(s) 1013
FbaI TGATCA 1 cut(s) 354
FokI GGATG 2 cut(s) 783, 1045
FriOI GRGCYC 1 cut(s) 899
FspBI CTAG 2 cut(s) 705, 993
GlaI GCGC 4 cut(s) 258, 314, 512, 1060
GsuI CTGGAG 2 cut(s) 140, 524
HaeIII GGCC 6 cut(s) 153, 380, 620, 703, 897, 942
HapII CCGG 3 cut(s) 381, 456, 689
HhaI GCGC 4 cut(s) 259, 315, 513, 1061
Hin1II CATG 6 cut(s) 89, 355, 475, 661, 947, 1019
Hin6I GCGC 4 cut(s) 257, 313, 511, 1059
HinP1I GCGC 4 cut(s) 257, 313, 511, 1059
HindIII AAGCTT 4 cut(s) 630, 856, 1177, 1259
HinfI GANTC 2 cut(s) 105, 1085
HpaII CCGG 3 cut(s) 381, 456, 689
Hpy188I TCNGA 2 cut(s) 177, 1193
Hpy188III TCNNGA 4 cut(s) 472, 532, 541, 572
HpyAV CCTTC 6 cut(s) 143, 157, 498, 589, 604, 1147
HpyCH4III ACNGT 3 cut(s) 559, 964, 1000
HpyCH4IV ACGT 1 cut(s) 46
HpyCH4V TGCA 9 cut(s) 218, 292, 392, 968, 980, 1160, 1165, 1202, 1257
HpyF3I CTNAG 5 cut(s) 246, 262, 338, 582, 1190
HpySE526I ACGT 1 cut(s) 46
Hsp92II CATG 6 cut(s) 89, 355, 475, 661, 947, 1019
HspAI GCGC 4 cut(s) 257, 313, 511, 1059
KroI GCCGGC 1 cut(s) 688
KroNI GCCGGC 1 cut(s) 690
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 6 cut(s) 354, 480, 574, 912, 926, 947
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 4 cut(s) 232, 505, 543, 1183
LweI GCATC 2 cut(s) 279, 1211
MaeI CTAG 2 cut(s) 705, 993
MaeII ACGT 1 cut(s) 46
MalI GATC 6 cut(s) 356, 482, 576, 914, 928, 949
MboI GATC 6 cut(s) 354, 480, 574, 912, 926, 947
MfeI CAATTG 1 cut(s) 1089
MflI RGATCY 2 cut(s) 480, 926
MhlI GDGCHC 1 cut(s) 899
MluCI AATT 6 cut(s) 549, 668, 680, 804, 1089, 1247
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 904, 1059
MroNI GCCGGC 1 cut(s) 688
MroXI GAANNNNTTC 2 cut(s) 171, 807
MseI TTAA 2 cut(s) 387, 866
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 3 cut(s) 381, 456, 689
MunI CAATTG 1 cut(s) 1089
Mva1269I GAATGC 2 cut(s) 465, 1009
MvnI CGCG 2 cut(s) 257, 917
NaeI GCCGGC 1 cut(s) 690
NcoI CCATGG 1 cut(s) 943
NdeII GATC 6 cut(s) 354, 480, 574, 912, 926, 947
NgoMIV GCCGGC 1 cut(s) 688
NheI GCTAGC 1 cut(s) 992
NlaIII CATG 6 cut(s) 89, 355, 475, 661, 947, 1019
NlaIV GGNNCC 3 cut(s) 655, 897, 928
NmeAIII GCCGAG 1 cut(s) 526
NspV TTCGAA 1 cut(s) 1226
PagI TCATGA 1 cut(s) 471
PceI AGGCCT 1 cut(s) 153
PciSI GCTCTTC 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 2 cut(s) 465, 1009
PdiI GCCGGC 1 cut(s) 690
PdmI GAANNNNTTC 2 cut(s) 171, 807
PfeI GAWTC 1 cut(s) 1085
Ple19I CGATCG 1 cut(s) 915
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PspN4I GGNNCC 3 cut(s) 655, 897, 928
PspOMI GGGCCC 1 cut(s) 895
PspPI GGNCC 3 cut(s) 619, 895, 896
PstI CTGCAG 2 cut(s) 220, 982
PstNI CAGNNNCTG 3 cut(s) 398, 977, 1056
PsuI RGATCY 2 cut(s) 480, 926
PvuI CGATCG 1 cut(s) 915
RgaI GCGATCGC 1 cut(s) 915
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 387, 866
Sau3AI GATC 6 cut(s) 354, 480, 574, 912, 926, 947
Sau96I GGNCC 3 cut(s) 619, 895, 896
SchI GAGTC 1 cut(s) 114
SduI GDGCHC 1 cut(s) 899
SfaAI GCGATCGC 1 cut(s) 915
SfaNI GCATC 2 cut(s) 279, 1211
SfcI CTRYAG 3 cut(s) 216, 231, 978
SfuI TTCGAA 1 cut(s) 1226
SgfI GCGATCGC 1 cut(s) 915
Sse9I AATT 6 cut(s) 549, 668, 680, 804, 1089, 1247
SseBI AGGCCT 1 cut(s) 153
SspMI CTAG 2 cut(s) 705, 993
StuI AGGCCT 1 cut(s) 153
StyI CCWWGG 2 cut(s) 731, 943
TaaI ACNGT 3 cut(s) 559, 964, 1000
TaiI ACGT 1 cut(s) 49
TaqI TCGA 4 cut(s) 147, 663, 1205, 1226
TasI AATT 6 cut(s) 549, 668, 680, 804, 1089, 1247
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 3 cut(s) 80, 398, 920
TfiI GAWTC 1 cut(s) 1085
Tru1I TTAA 2 cut(s) 387, 866
Tru9I TTAA 2 cut(s) 387, 866
TscAI CASTG 2 cut(s) 978, 1005
TspDTI ATGAA 5 cut(s) 9, 74, 417, 488, 1166
TspGWI ACGGA 3 cut(s) 397, 633, 1245
TspRI CASTG 2 cut(s) 978, 1005
XapI RAATTY 3 cut(s) 668, 680, 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmnI GAANNNNTTC 2 cut(s) 171, 807
XspI CTAG 2 cut(s) 705, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.