pycom10g27420

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009170
Physical Location & Seq
Reverse (-)
28382957 .. 28384213
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27420.1

Sequence Viewer

Length: 1257 bp
ATGGCATCTCTCCTCTTCTCTTCTCCCTCACGTTGTTCTTCCTCCAACAGAACCATAAATGCGGCCATTCATGTCCCCAAGCTCCCAAGAGTCCGCTTCACTGTCCCCAAACAAACGACGAAGCTCGTCCTCGAAGGCCTCCAGTTGAAGGAACAAGTTCTGAAACACGAAAACATCGATTTGTATAATAACAATCAGTCTGCAGCAGCAGCAACTCCTACAGCTTCCACAGCTCAGCTCTACGCGCTCTTAGAGGCGGTGGCGGACAGAGTGGAGATGCACGCAAACATTGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCACTCTCTCCGCTTCCCTCATGGCGGGAGTTAATGCAGCTGCTGGAACGTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCCACCGGAATGCTGCTTATCATGAACAAGATCCAGCCTTCGCAGCTCGCCGAGGAGCAGCGCAACGCAACGAGGTTGTTCAGGCAGCTCCAGAGTGAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGGTTTTGGTTCTTGACAAAGCTTACCCTCTTCCGTTACTTGGTGCCATGCTCGAAAAATTCCCCAAAAAATTTGAGCCGGCTGTTTGGTGGCCTAGAAATGTTGTTCAATCCCAAAATCCAAGGAAACTGAAATCCCAGAAGGGAAAGAATGTGAATGGATGGAGTGAAGAACTTGAAAGTGAAATGAGAGAAATTATTCAAGTTGTGAAGAGCAAGGACATTGAAGACTATGAGAGATTAGGGAGCTTGGTTCTAAAGATCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGGGATTGCGGCTGTTGGATCCACTTTTGTAGGCCATGGATCAGCCACTGCCGCGGTTGCAGCAGTGGCTGCAGGCTCGTTGGCTAGCGCAGTGAATGCTTTTCAGCACGGCGGGCAAGTAGGGATGGTATTTGAGATGTATAGAAACTGCGCCGGCTTCTTCAACAAGTTAGAAGATTCAATTGAAGCAGCACTTGGAGAACAAGACTTGGAGAAGAGAGAAAATGGGGGGATTTTCGAAATGAAGGTTGCAATGCAGTTGGGAAGAAGCTTGGAGCAGCTCAGAGAACTTGCATCGAAATCAGCTTTGTATCGTTCGAATGGAGAACCCATAGACGAATTCGGCTGCAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

419

Amino Acids

45.62

Weight (kDa)

8.28

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 79 - 394 6.2e-155 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 641
AccII CGCG 2 cut(s) 245, 950
AclWI GGATC 5 cut(s) 463, 557, 909, 922, 943
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 3 cut(s) 656, 668, 1235
AfiI CCNNNNNNNGG 3 cut(s) 148, 367, 638
AgsI TTSAA 9 cut(s) 148, 409, 707, 776, 800, 824, 1060, 1077, 1082
AjuI GAANNNNNNNTTGG 2 cut(s) 1074, 1106
Alw26I GTCTC 2 cut(s) 536, 581
AlwI GGATC 5 cut(s) 463, 557, 909, 922, 943
AlwNI CAGNNNCTG 3 cut(s) 386, 944, 965
AoxI GGCC 5 cut(s) 63, 136, 689, 883, 928
ApaI GGGCCC 1 cut(s) 887
ApoI RAATTY 3 cut(s) 656, 668, 1235
Asp700I GAANNNNTTC 2 cut(s) 156, 795
AspLEI GCGC 5 cut(s) 247, 303, 501, 986, 1049
AspS9I GGNCC 2 cut(s) 883, 884
AsuII TTCGAA 2 cut(s) 1134, 1214
AsuNHI GCTAGC 1 cut(s) 980
BaeGI GKGCMC 1 cut(s) 887
BamHI GGATCC 1 cut(s) 914
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 887
BbsI GAAGAC 1 cut(s) 831
BccI CCATC 2 cut(s) 753, 1015
BceAI ACGGC 1 cut(s) 1021
BclI TGATCA 1 cut(s) 342
BcoDI GTCTC 2 cut(s) 536, 581
BfaI CTAG 2 cut(s) 693, 981
BfmI CTRYAG 3 cut(s) 201, 219, 966
BlpI GCTNAGC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 883, 884
BmiI GGNNCC 3 cut(s) 643, 885, 916
BmrI ACTGGG 1 cut(s) 906
BmsI GCATC 3 cut(s) 14, 267, 1199
BmtI GCTAGC 1 cut(s) 984
BmuI ACTGGG 1 cut(s) 906
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 2 cut(s) 125, 512
Bpu1102I GCTNAGC 1 cut(s) 234
Bpu14I TTCGAA 2 cut(s) 1134, 1214
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 489, 719, 931, 948
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 367, 638
Bse118I RCCGGY 2 cut(s) 676, 1049
Bse1I ACTGG 3 cut(s) 142, 314, 901
Bse3DI GCAATG 1 cut(s) 1155
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 4 cut(s) 489, 719, 931, 948
BseGI GGATG 2 cut(s) 764, 1026
BseLI CCNNNNNNNGG 3 cut(s) 148, 367, 638
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 3 cut(s) 248, 561, 1192
BseNI ACTGG 3 cut(s) 142, 314, 901
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 887
Bsh1236I CGCG 2 cut(s) 245, 950
BshFI GGCC 5 cut(s) 65, 138, 691, 885, 930
BshNI GGYRCC 1 cut(s) 641
BshVI ATCGAT 1 cut(s) 177
BsiSI CCGG 3 cut(s) 444, 677, 1050
BslFI GGGAC 2 cut(s) 59, 89
BslI CCNNNNNNNGG 3 cut(s) 148, 367, 638
BsmAI GTCTC 2 cut(s) 536, 581
BsmBI CGTCTC 1 cut(s) 536
BsmFI GGGAC 2 cut(s) 59, 89
BsmI GAATGC 2 cut(s) 453, 997
BsnI GGCC 5 cut(s) 65, 138, 691, 885, 930
Bsp119I TTCGAA 2 cut(s) 1134, 1214
Bsp120I GGGCCC 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 887
Bsp143I GATC 6 cut(s) 342, 468, 562, 858, 914, 935
Bsp1720I GCTNAGC 1 cut(s) 234
Bsp19I CCATGG 1 cut(s) 931
BspANI GGCC 5 cut(s) 65, 138, 691, 885, 930
BspCNI CTCAG 3 cut(s) 247, 562, 1191
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 2 cut(s) 245, 950
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 3 cut(s) 643, 885, 916
BspMAI CTGCAG 2 cut(s) 205, 970
BspOI GCTAGC 1 cut(s) 984
BspPI GGATC 5 cut(s) 463, 557, 909, 922, 943
BspQI GCTCTTC 2 cut(s) 561, 803
BspT104I TTCGAA 2 cut(s) 1134, 1214
BspT107I GGYRCC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 1155
BsrFI RCCGGY 2 cut(s) 676, 1049
BsrI ACTGG 3 cut(s) 142, 314, 901
BssAI RCCGGY 2 cut(s) 676, 1049
BssECI CCNNGG 4 cut(s) 489, 719, 931, 948
BssMI GATC 6 cut(s) 342, 468, 562, 858, 914, 935
BssT1I CCWWGG 2 cut(s) 719, 931
Bst4CI ACNGT 2 cut(s) 103, 547
Bst6I CTCTTC 6 cut(s) 20, 25, 561, 633, 803, 1106
BstAPI GCANNNNNTGC 2 cut(s) 965, 992
BstBI TTCGAA 2 cut(s) 1134, 1214
BstC8I GCNNGC 9 cut(s) 282, 296, 486, 678, 970, 982, 1010, 1051, 1247
BstDEI CTNAG 5 cut(s) 234, 250, 326, 570, 1178
BstDSI CCRYGG 2 cut(s) 931, 948
BstF5I GGATG 2 cut(s) 764, 1026
BstFNI CGCG 2 cut(s) 245, 950
BstHHI GCGC 5 cut(s) 247, 303, 501, 986, 1049
BstKTI GATC 6 cut(s) 345, 471, 565, 861, 917, 938
BstMAI GTCTC 2 cut(s) 536, 581
BstMBI GATC 6 cut(s) 342, 468, 562, 858, 914, 935
BstSFI CTRYAG 3 cut(s) 201, 219, 966
BstSLI GKGCMC 1 cut(s) 887
BstUI CGCG 2 cut(s) 245, 950
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 2 cut(s) 468, 914
BstYI RGATCY 2 cut(s) 468, 914
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 5 cut(s) 65, 138, 691, 885, 930
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 931, 948
BtsCI GGATG 2 cut(s) 764, 1026
BtsI GCAGTG 3 cut(s) 942, 966, 993
BtsIMutI CAGTG 4 cut(s) 99, 942, 966, 993
Cac8I GCNNGC 9 cut(s) 282, 296, 486, 678, 970, 982, 1010, 1051, 1247
CaiI CAGNNNCTG 3 cut(s) 386, 944, 965
CciI TCATGA 1 cut(s) 459
CfoI GCGC 5 cut(s) 247, 303, 501, 986, 1049
Cfr10I RCCGGY 2 cut(s) 676, 1049
Cfr13I GGNCC 2 cut(s) 883, 884
Cfr42I CCGCGG 1 cut(s) 951
ClaI ATCGAT 1 cut(s) 177
CviAII CATG 6 cut(s) 71, 340, 364, 460, 646, 932
DdeI CTNAG 5 cut(s) 234, 250, 326, 570, 1178
DpnI GATC 6 cut(s) 344, 470, 564, 860, 916, 937
DpnII GATC 6 cut(s) 342, 468, 562, 858, 914, 935
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 6 cut(s) 20, 25, 561, 633, 803, 1106
EarI CTCTTC 6 cut(s) 20, 25, 561, 633, 803, 1106
EciI GGCGGA 1 cut(s) 278
Eco130I CCWWGG 2 cut(s) 719, 931
Eco147I AGGCCT 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 887
EcoO109I RGGNCCY 1 cut(s) 884
EcoRI GAATTC 1 cut(s) 1235
EcoT14I CCWWGG 2 cut(s) 719, 931
EcoT38I GRGCYC 1 cut(s) 887
ErhI CCWWGG 2 cut(s) 719, 931
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 6 cut(s) 74, 343, 367, 463, 649, 935
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FaqI GGGAC 2 cut(s) 59, 89
FatI CATG 6 cut(s) 70, 339, 363, 459, 645, 931
FauI CCCGC 2 cut(s) 361, 1001
FbaI TGATCA 1 cut(s) 342
FokI GGATG 2 cut(s) 771, 1033
FriOI GRGCYC 1 cut(s) 887
FspBI CTAG 2 cut(s) 693, 981
GlaI GCGC 5 cut(s) 246, 302, 500, 985, 1048
GsuI CTGGAG 2 cut(s) 125, 512
HaeIII GGCC 5 cut(s) 65, 138, 691, 885, 930
HapII CCGG 3 cut(s) 444, 677, 1050
HhaI GCGC 5 cut(s) 247, 303, 501, 986, 1049
Hin1II CATG 6 cut(s) 74, 343, 367, 463, 649, 935
Hin6I GCGC 5 cut(s) 245, 301, 499, 984, 1047
HinP1I GCGC 5 cut(s) 245, 301, 499, 984, 1047
HindIII AAGCTT 3 cut(s) 618, 1165, 1247
HinfI GANTC 2 cut(s) 90, 1073
HpaII CCGG 3 cut(s) 444, 677, 1050
Hpy188I TCNGA 2 cut(s) 162, 1181
Hpy188III TCNNGA 4 cut(s) 460, 529, 560, 611
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 7 cut(s) 128, 142, 486, 577, 592, 733, 1135
HpyCH4III ACNGT 2 cut(s) 103, 547
HpyCH4IV ACGT 2 cut(s) 31, 392
HpyCH4V TGCA 9 cut(s) 203, 280, 380, 956, 968, 1148, 1153, 1190, 1245
HpyF3I CTNAG 5 cut(s) 234, 250, 326, 570, 1178
HpySE526I ACGT 2 cut(s) 31, 392
Hsp92II CATG 6 cut(s) 74, 343, 367, 463, 649, 935
HspAI GCGC 5 cut(s) 245, 301, 499, 984, 1047
KroI GCCGGC 2 cut(s) 676, 1049
KroNI GCCGGC 2 cut(s) 678, 1051
Ksp22I TGATCA 1 cut(s) 342
KspI CCGCGG 1 cut(s) 951
Kzo9I GATC 6 cut(s) 342, 468, 562, 858, 914, 935
LguI GCTCTTC 2 cut(s) 561, 803
LmnI GCTCC 5 cut(s) 87, 493, 531, 843, 1171
LweI GCATC 3 cut(s) 14, 267, 1199
MaeI CTAG 2 cut(s) 693, 981
MaeII ACGT 2 cut(s) 31, 392
MaeIII GTNAC 1 cut(s) 633
MalI GATC 6 cut(s) 344, 470, 564, 860, 916, 937
MboI GATC 6 cut(s) 342, 468, 562, 858, 914, 935
MfeI CAATTG 1 cut(s) 1077
MflI RGATCY 2 cut(s) 468, 914
MhlI GDGCHC 1 cut(s) 887
MluCI AATT 6 cut(s) 537, 656, 668, 792, 1077, 1235
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 69, 892
MroNI GCCGGC 2 cut(s) 676, 1049
MroXI GAANNNNTTC 2 cut(s) 156, 795
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 446
MspA1I CMGCKG 2 cut(s) 383, 950
MspI CCGG 3 cut(s) 444, 677, 1050
MunI CAATTG 1 cut(s) 1077
Mva1269I GAATGC 2 cut(s) 453, 997
MvnI CGCG 2 cut(s) 245, 950
NaeI GCCGGC 2 cut(s) 678, 1051
NcoI CCATGG 1 cut(s) 931
NdeII GATC 6 cut(s) 342, 468, 562, 858, 914, 935
NgoMIV GCCGGC 2 cut(s) 676, 1049
NheI GCTAGC 1 cut(s) 980
NlaIII CATG 6 cut(s) 74, 343, 367, 463, 649, 935
NlaIV GGNNCC 3 cut(s) 643, 885, 916
NmeAIII GCCGAG 1 cut(s) 514
NspV TTCGAA 2 cut(s) 1134, 1214
PagI TCATGA 1 cut(s) 459
PceI AGGCCT 1 cut(s) 138
PciSI GCTCTTC 2 cut(s) 561, 803
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 2 cut(s) 453, 997
PdiI GCCGGC 2 cut(s) 678, 1051
PdmI GAANNNNTTC 2 cut(s) 156, 795
PfeI GAWTC 1 cut(s) 1073
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 643, 885, 916
PspOMI GGGCCC 1 cut(s) 883
PspPI GGNCC 2 cut(s) 883, 884
PstI CTGCAG 2 cut(s) 205, 970
PstNI CAGNNNCTG 3 cut(s) 386, 944, 965
PsuI RGATCY 2 cut(s) 468, 914
PvuII CAGCTG 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 446
SacII CCGCGG 1 cut(s) 951
SapI GCTCTTC 2 cut(s) 561, 803
SaqAI TTAA 1 cut(s) 375
Sau3AI GATC 6 cut(s) 342, 468, 562, 858, 914, 935
Sau96I GGNCC 2 cut(s) 883, 884
SchI GAGTC 1 cut(s) 99
SduI GDGCHC 1 cut(s) 887
SfaNI GCATC 3 cut(s) 14, 267, 1199
SfcI CTRYAG 3 cut(s) 201, 219, 966
Sfr303I CCGCGG 1 cut(s) 951
SfuI TTCGAA 2 cut(s) 1134, 1214
SgrBI CCGCGG 1 cut(s) 951
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 6 cut(s) 537, 656, 668, 792, 1077, 1235
SseBI AGGCCT 1 cut(s) 138
SspMI CTAG 2 cut(s) 693, 981
StuI AGGCCT 1 cut(s) 138
StyI CCWWGG 2 cut(s) 719, 931
TaaI ACNGT 2 cut(s) 103, 547
TaiI ACGT 2 cut(s) 34, 395
TaqI TCGA 6 cut(s) 132, 177, 651, 1134, 1193, 1214
TasI AATT 6 cut(s) 537, 656, 668, 792, 1077, 1235
TauI GCSGC 3 cut(s) 65, 908, 950
TfiI GAWTC 1 cut(s) 1073
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 4 cut(s) 106, 949, 966, 993
TspDTI ATGAA 4 cut(s) 59, 405, 476, 1154
TspGWI ACGGA 2 cut(s) 385, 621
TspRI CASTG 4 cut(s) 106, 949, 966, 993
XapI RAATTY 3 cut(s) 656, 668, 1235
XmnI GAANNNNTTC 2 cut(s) 156, 795
XspI CTAG 2 cut(s) 693, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.