pycom05g31650

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009170
Physical Location & Seq
Reverse (-)
31312086 .. 31313066
981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31650.1

Sequence Viewer

Length: 981 bp
ATGCACGCAAACATCGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCACTCTCTCCGCTTCCCTAATGGCCGGAGTTAATGCAGCTGCTGGAACGTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCTACCGGAATGCTGCTTATCATGAACAAGATCCAGCCTTCGCAGCTCGCCGAGGAGCAGCGCAATGCTACGAGGTTGTTCAGGCAGCTCCAGAGCGAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGGTTTTGGCTCTTGACAAAGCTTACCCACTTCCGTTGCTCGGTGCCATGCTCGAAAAATTCCCCAAAAAATTTGAGCCGGCCGTTTGGTGGCCTAGAAATGTTGTTCAATCTCAAAATCCAAGGAAACCGAAATCCCAGATGGGAAATAATGTGAATGGATGGAGTGAAGAACTTGAAAGCGAAATGAGAGAAATTATTCAAGTTGTGAAGAACAAGGACATTGAAGACTATGAGAGATTAGGAAGCTTGGCATTAAGGACCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGGGATCGCGGCTGTTGGATCCGCTTTTGTAGGCCATGGATCAGCCACTGCCGCGGTTGCAGCAGTGGCTGCAGGCTCTTTGGCTAGCGCAGTGAATGCTTTTCAGCATGGCGGGCAAGTAGGGATGGTGTTTGAGATGTATAGGAACTGCGCCGGCTTCTTCAACAGGTTGGAGGATTCAATTGAAGCAACACTTGGAGAACAAGACTGGGAGAAGAGAGAAAATGGGGTGGTTTTTGAAATGAAGGTTGCAATGCGGTTGGGAAGAAGCTTGGAGCAGCTCAGAGAACTTGCATCGAAATCAGCTTTGTGTCGTTCGAACGGAGAACCCATAGAGGAATTTGGTTGCAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

327

Amino Acids

35.66

Weight (kDa)

6.07

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 1 - 302 5.6e-147 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 2 cut(s) 629, 674
AciI CCGC 8 cut(s) 78, 159, 629, 642, 672, 674, 732, 877
AclWI GGATC 6 cut(s) 187, 281, 632, 633, 646, 667
AcoI YGGCCR 2 cut(s) 90, 402
AcsI RAATTY 3 cut(s) 380, 392, 959
AfiI CCNNNNNNNGG 2 cut(s) 362, 411
AgsI TTSAA 9 cut(s) 133, 431, 500, 524, 548, 784, 801, 806, 860
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
Alw26I GTCTC 2 cut(s) 260, 305
AlwI GGATC 6 cut(s) 187, 281, 632, 633, 646, 667
AlwNI CAGNNNCTG 3 cut(s) 110, 668, 689
AoxI GGCC 5 cut(s) 90, 402, 413, 607, 652
ApaI GGGCCC 1 cut(s) 611
ApoI RAATTY 3 cut(s) 380, 392, 959
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 4 cut(s) 27, 225, 710, 773
AspS9I GGNCC 3 cut(s) 582, 607, 608
AsuII TTCGAA 1 cut(s) 938
AsuNHI GCTAGC 1 cut(s) 704
AvaII GGWCC 1 cut(s) 582
BaeGI GKGCMC 1 cut(s) 611
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 555
BccI CCATC 3 cut(s) 457, 477, 739
BceAI ACGGC 1 cut(s) 389
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 260, 305
BfaI CTAG 2 cut(s) 417, 705
BfmI CTRYAG 1 cut(s) 690
Bme18I GGWCC 1 cut(s) 582
BmgT120I GGNCC 3 cut(s) 582, 607, 608
BmiI GGNNCC 3 cut(s) 367, 609, 640
BmrI ACTGGG 2 cut(s) 630, 838
BmsI GCATC 1 cut(s) 923
BmtI GCTAGC 1 cut(s) 708
BmuI ACTGGG 2 cut(s) 630, 838
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 938
BsaJI CCNNGG 4 cut(s) 213, 443, 655, 672
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 411
Bse118I RCCGGY 2 cut(s) 400, 773
Bse1I ACTGG 3 cut(s) 38, 625, 833
Bse3DI GCAATG 2 cut(s) 232, 879
BseDI CCNNGG 4 cut(s) 213, 443, 655, 672
BseGI GGATG 2 cut(s) 488, 750
BseLI CCNNNNNNNGG 2 cut(s) 362, 411
BseMI GCAATG 2 cut(s) 232, 879
BseMII CTCAG 2 cut(s) 285, 916
BseNI ACTGG 3 cut(s) 38, 625, 833
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 1 cut(s) 611
BseX3I CGGCCG 1 cut(s) 402
Bsh1236I CGCG 2 cut(s) 629, 674
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 5 cut(s) 92, 404, 415, 609, 654
BshNI GGYRCC 1 cut(s) 365
BsiEI CGRYCG 1 cut(s) 405
BsiSI CCGG 4 cut(s) 93, 168, 401, 774
BslI CCNNNNNNNGG 2 cut(s) 362, 411
BsmAI GTCTC 2 cut(s) 260, 305
BsmBI CGTCTC 1 cut(s) 260
BsmI GAATGC 2 cut(s) 177, 721
BsnI GGCC 5 cut(s) 92, 404, 415, 609, 654
Bsp119I TTCGAA 1 cut(s) 938
Bsp120I GGGCCC 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 6 cut(s) 66, 192, 286, 624, 638, 659
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 8 cut(s) 78, 159, 629, 642, 672, 674, 732, 877
BspANI GGCC 5 cut(s) 92, 404, 415, 609, 654
BspCNI CTCAG 2 cut(s) 286, 915
BspFNI CGCG 2 cut(s) 629, 674
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 367, 609, 640
BspMAI CTGCAG 1 cut(s) 694
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 6 cut(s) 187, 281, 632, 633, 646, 667
BspQI GCTCTTC 1 cut(s) 285
BspT104I TTCGAA 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 232, 879
BsrFI RCCGGY 2 cut(s) 400, 773
BsrI ACTGG 3 cut(s) 38, 625, 833
BssAI RCCGGY 2 cut(s) 400, 773
BssECI CCNNGG 4 cut(s) 213, 443, 655, 672
BssMI GATC 6 cut(s) 66, 192, 286, 624, 638, 659
BssT1I CCWWGG 2 cut(s) 443, 655
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 2 cut(s) 285, 830
BstAPI GCANNNNNTGC 2 cut(s) 689, 716
BstBI TTCGAA 1 cut(s) 938
BstC8I GCNNGC 9 cut(s) 6, 20, 210, 402, 694, 706, 734, 775, 971
BstDEI CTNAG 3 cut(s) 50, 294, 902
BstDSI CCRYGG 2 cut(s) 655, 672
BstF5I GGATG 2 cut(s) 488, 750
BstFNI CGCG 2 cut(s) 629, 674
BstHHI GCGC 4 cut(s) 27, 225, 710, 773
BstKTI GATC 6 cut(s) 69, 195, 289, 627, 641, 662
BstMAI GTCTC 2 cut(s) 260, 305
BstMBI GATC 6 cut(s) 66, 192, 286, 624, 638, 659
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 9 cut(s) 24, 205, 671, 677, 680, 686, 689, 716, 733
BstSFI CTRYAG 1 cut(s) 690
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 2 cut(s) 629, 674
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 2 cut(s) 192, 638
BstYI RGATCY 2 cut(s) 192, 638
BstZI CGGCCG 1 cut(s) 402
BsuRI GGCC 5 cut(s) 92, 404, 415, 609, 654
BtgI CCRYGG 2 cut(s) 655, 672
BtsCI GGATG 2 cut(s) 488, 750
BtsI GCAGTG 3 cut(s) 666, 690, 717
BtsIMutI CAGTG 3 cut(s) 666, 690, 717
Cac8I GCNNGC 9 cut(s) 6, 20, 210, 402, 694, 706, 734, 775, 971
CaiI CAGNNNCTG 3 cut(s) 110, 668, 689
CciI TCATGA 1 cut(s) 183
CfoI GCGC 4 cut(s) 27, 225, 710, 773
Cfr10I RCCGGY 2 cut(s) 400, 773
Cfr13I GGNCC 3 cut(s) 582, 607, 608
Cfr42I CCGCGG 1 cut(s) 675
CspCI CAANNNNNGTGG 2 cut(s) 339, 374
CviAII CATG 5 cut(s) 64, 184, 370, 656, 728
DdeI CTNAG 3 cut(s) 50, 294, 902
DpnI GATC 6 cut(s) 68, 194, 288, 626, 640, 661
DpnII GATC 6 cut(s) 66, 192, 286, 624, 638, 659
EaeI YGGCCR 2 cut(s) 90, 402
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 285, 830
EarI CTCTTC 2 cut(s) 285, 830
EclXI CGGCCG 1 cut(s) 402
Eco130I CCWWGG 2 cut(s) 443, 655
Eco24I GRGCYC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 582
Eco52I CGGCCG 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 608
EcoT14I CCWWGG 2 cut(s) 443, 655
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 2 cut(s) 443, 655
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 5 cut(s) 67, 187, 373, 659, 731
FaiI YATR 8 cut(s) 65, 185, 371, 555, 657, 729, 762, 953
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FatI CATG 5 cut(s) 63, 183, 369, 655, 727
FauI CCCGC 1 cut(s) 725
FbaI TGATCA 1 cut(s) 66
FokI GGATG 2 cut(s) 495, 757
FriOI GRGCYC 1 cut(s) 611
FspBI CTAG 2 cut(s) 417, 705
GlaI GCGC 4 cut(s) 26, 224, 709, 772
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 5 cut(s) 92, 404, 415, 609, 654
HapII CCGG 4 cut(s) 93, 168, 401, 774
HhaI GCGC 4 cut(s) 27, 225, 710, 773
Hin1II CATG 5 cut(s) 67, 187, 373, 659, 731
Hin6I GCGC 4 cut(s) 25, 223, 708, 771
HinP1I GCGC 4 cut(s) 25, 223, 708, 771
HindIII AAGCTT 4 cut(s) 342, 568, 889, 971
HinfI GANTC 1 cut(s) 797
HpaII CCGG 4 cut(s) 93, 168, 401, 774
Hpy188I TCNGA 1 cut(s) 905
Hpy188III TCNNGA 4 cut(s) 184, 253, 284, 335
HpyAV CCTTC 4 cut(s) 210, 301, 316, 859
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 7 cut(s) 4, 104, 680, 692, 872, 914, 969
HpyF10VI GCNNNNNNNGC 9 cut(s) 24, 205, 671, 677, 680, 686, 689, 716, 733
HpyF3I CTNAG 3 cut(s) 50, 294, 902
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 5 cut(s) 67, 187, 373, 659, 731
HspAI GCGC 4 cut(s) 25, 223, 708, 771
KroI GCCGGC 2 cut(s) 400, 773
KroNI GCCGGC 2 cut(s) 402, 775
Ksp22I TGATCA 1 cut(s) 66
KspI CCGCGG 1 cut(s) 675
Kzo9I GATC 6 cut(s) 66, 192, 286, 624, 638, 659
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 3 cut(s) 217, 255, 895
LweI GCATC 1 cut(s) 923
MaeI CTAG 2 cut(s) 417, 705
MaeII ACGT 1 cut(s) 116
MalI GATC 6 cut(s) 68, 194, 288, 626, 640, 661
MboI GATC 6 cut(s) 66, 192, 286, 624, 638, 659
MboII GAAGA 7 cut(s) 272, 503, 544, 560, 772, 847, 897
MfeI CAATTG 1 cut(s) 801
MflI RGATCY 2 cut(s) 192, 638
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 6 cut(s) 261, 380, 392, 516, 801, 959
MmeI TCCRAC 2 cut(s) 616, 771
MnlI CCTC 4 cut(s) 208, 228, 787, 949
MroNI GCCGGC 2 cut(s) 400, 773
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 2 cut(s) 99, 578
MspA1I CMGCKG 2 cut(s) 107, 674
MspI CCGG 4 cut(s) 93, 168, 401, 774
MunI CAATTG 1 cut(s) 801
Mva1269I GAATGC 2 cut(s) 177, 721
MvnI CGCG 2 cut(s) 629, 674
MwoI GCNNNNNNNGC 9 cut(s) 24, 205, 671, 677, 680, 686, 689, 716, 733
NaeI GCCGGC 2 cut(s) 402, 775
NcoI CCATGG 1 cut(s) 655
NdeII GATC 6 cut(s) 66, 192, 286, 624, 638, 659
NgoMIV GCCGGC 2 cut(s) 400, 773
NheI GCTAGC 1 cut(s) 704
NlaIII CATG 5 cut(s) 67, 187, 373, 659, 731
NlaIV GGNNCC 3 cut(s) 367, 609, 640
NmeAIII GCCGAG 1 cut(s) 238
NspV TTCGAA 1 cut(s) 938
PagI TCATGA 1 cut(s) 183
PciSI GCTCTTC 1 cut(s) 285
PctI GAATGC 2 cut(s) 177, 721
PdiI GCCGGC 2 cut(s) 402, 775
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 797
PspN4I GGNNCC 3 cut(s) 367, 609, 640
PspOMI GGGCCC 1 cut(s) 607
PspPI GGNCC 3 cut(s) 582, 607, 608
PstI CTGCAG 1 cut(s) 694
PstNI CAGNNNCTG 3 cut(s) 110, 668, 689
PsuI RGATCY 2 cut(s) 192, 638
PvuII CAGCTG 1 cut(s) 107
SacII CCGCGG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 2 cut(s) 99, 578
Sau3AI GATC 6 cut(s) 66, 192, 286, 624, 638, 659
Sau96I GGNCC 3 cut(s) 582, 607, 608
SduI GDGCHC 1 cut(s) 611
SfaNI GCATC 1 cut(s) 923
SfcI CTRYAG 1 cut(s) 690
Sfr303I CCGCGG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 938
SgrBI CCGCGG 1 cut(s) 675
SinI GGWCC 1 cut(s) 582
Sse9I AATT 6 cut(s) 261, 380, 392, 516, 801, 959
SsiI CCGC 8 cut(s) 78, 159, 629, 642, 672, 674, 732, 877
SspMI CTAG 2 cut(s) 417, 705
StyI CCWWGG 2 cut(s) 443, 655
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 1 cut(s) 119
TaqI TCGA 3 cut(s) 375, 917, 938
TasI AATT 6 cut(s) 261, 380, 392, 516, 801, 959
TauI GCSGC 2 cut(s) 632, 674
TfiI GAWTC 1 cut(s) 797
Tru1I TTAA 2 cut(s) 99, 578
Tru9I TTAA 2 cut(s) 99, 578
TscAI CASTG 3 cut(s) 673, 690, 717
TspDTI ATGAA 3 cut(s) 129, 200, 878
TspGWI ACGGA 3 cut(s) 109, 345, 957
TspRI CASTG 3 cut(s) 673, 690, 717
VpaK11BI GGWCC 1 cut(s) 582
XapI RAATTY 3 cut(s) 380, 392, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 417, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.