AT4G22030

F-box protein At4g22030

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009170
Physical Location & Seq
Forward (+)
11672349 .. 11673748
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22030.1

Sequence Viewer

Length: 1257 bp
ATGGCGTCATTACAGGCTTCAAGATTTATGCTTACTACTTCTTGTTCTTCTTGCACAAGAACAAGATCCGCAATCTCAATCCCAAAGTTACCAAAATTTAATGTCTCGATTCCGCAAATACCGATCAAGAACCGGAATTTCAGAGTCGAAGAACCGATTCTTCGGTTGGATGGGTTTGCTGAAGATCTTGATCTTTCGTTGTCAGAGAGACAAAGGCAAAAGAAGAGAGGGTGCGATGATCAGGTTTTGCAAAGGTCGCGGCTCATGGCGGTTCTTGAAGAAGTTATAGATAGAGTGGAGATGCACAAGAACATTGGAGAGCAACGCAATAACTGGAACTCTCTCTTGCTCAATTCCGTCAATATGATCACTCTCACTGCCGCTTTGATGGCCGGAATAGCCTCTATGAACGCCAGTGGAATAGATTCCGTTTCCGCGGTGAGTATAGCTTCTACGGTGTTGCTGGCTTCTGCTACAGGTTTGGCTGCCTTGATGAACAAGATTCAGCCTTCACAGCTTGTGGAGGAGCAGAGGAACGCAACGAGGCTGTTCAAGCAGCTAAGAAACAGAATCGAGATGGTTCTGAGGGAGAAAAGTGAAGATGGAATCAGTGAAGAAGACGTGAAAGAAGCGATAAAGAGAGTGCTCTGTTTGGACAAAGCTTACCCACTTCCTCTAGTTGGCACCATGCTCGAGAAGTTCCCTCAAGAATTCAAGCCAGCGACTTGGTGGCCTGAAACAAAACCCGAACCCACTCATTCCAGAACGGAAGCTAACGGGTGGAACTCGGAGCTAGAGATGGAGATGAGAGAGGTGGTTGAGGTAATTAAGAGTAGAGATGCAGAGGAGTACGAGAAGTTAGGTAACGTGGCCTTGAAGTTAAACCGGGTTTTGGCAATCTCTGGACCAGTGTTGACCGGAATTGCTGCCGTAAGTTCCGGTTTTATAGGTCACGGTTCAGGTTTAGCTGGAGTAGTAGCAACAACGTGTGCTTCATTGGCTGCCGTAGTGAACACGTTAGAGCACGGAGGTCAAGTAGGGATGGTGGTTGAGATGTATAGAAACTCCGCCGGATTCTTCTCTCTTATGGAAGATACGATCAAAACAGAGAGACGAGAGAATGGACAAGTTTTCGAGACCAAAGTAGCGTTGAAACTTGGGAGAAGCTTGTCTGAATTGAGAGATCTGGCCAGAAGATCTAGCTTGTCTAGGTTACAAGGATCAGCCATAGATGAATGCGCAAGCAAGCTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

418

Amino Acids

46.18

Weight (kDa)

8.15

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 87 - 394 5.1e-139 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1240
AccB1I GGYRCC 1 cut(s) 683
AccII CGCG 2 cut(s) 259, 437
AciI CCGC 8 cut(s) 69, 113, 259, 269, 381, 435, 437, 1068
AclWI GGATC 2 cut(s) 60, 1228
AcoI YGGCCR 2 cut(s) 390, 1188
AcsI RAATTY 3 cut(s) 95, 136, 710
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 851
AfiI CCNNNNNNNGG 2 cut(s) 680, 892
AflIII ACRYGT 2 cut(s) 986, 1014
AgsI TTSAA 6 cut(s) 21, 278, 553, 715, 877, 1153
AjiI CACGTC 1 cut(s) 622
Alw21I GWGCWC 2 cut(s) 648, 1026
Alw26I GTCTC 4 cut(s) 109, 202, 1105, 1130
AlwI GGATC 2 cut(s) 60, 1228
Ama87I CYCGRG 1 cut(s) 692
AoxI GGCC 4 cut(s) 390, 731, 870, 1188
ApeKI GCWGC 4 cut(s) 485, 556, 926, 1001
ApoI RAATTY 3 cut(s) 95, 136, 710
Asp700I GAANNNNTTC 2 cut(s) 156, 424
AspLEI GCGC 1 cut(s) 1241
AspS9I GGNCC 1 cut(s) 905
AsuC2I CCSGG 1 cut(s) 887
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 692
AvaII GGWCC 1 cut(s) 905
BalI TGGCCA 1 cut(s) 1190
BanI GGYRCC 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 648, 1026
BbvI GCAGC 4 cut(s) 472, 568, 913, 988
BccI CCATC 6 cut(s) 164, 382, 571, 596, 793, 1036
BceAI ACGGC 2 cut(s) 914, 989
BcgI CGANNNNNNTGC 2 cut(s) 673, 707
BclI TGATCA 2 cut(s) 238, 366
BcnI CCSGG 1 cut(s) 887
BcoDI GTCTC 4 cut(s) 109, 202, 1105, 1130
BfaI CTAG 4 cut(s) 677, 794, 1200, 1209
BfmI CTRYAG 1 cut(s) 474
BglII AGATCT 3 cut(s) 184, 1183, 1196
BisI GCNGC 6 cut(s) 260, 381, 486, 557, 927, 1002
BlsI GCNGC 6 cut(s) 261, 382, 487, 558, 928, 1003
Bme1390I CCNGG 1 cut(s) 887
Bme18I GGWCC 1 cut(s) 905
BmeT110I CYCGRG 1 cut(s) 692
BmgBI CACGTC 1 cut(s) 622
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 1 cut(s) 887
BmsI GCATC 2 cut(s) 291, 829
BpiI GAAGAC 1 cut(s) 624
BpmI CTGGAG 1 cut(s) 990
BpuEI CTTGAG 1 cut(s) 690
BpuMI CCSGG 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1130
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 3 cut(s) 132, 917, 938
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 2 cut(s) 680, 892
Bse1I ACTGG 3 cut(s) 338, 414, 908
Bse8I GATNNNNATC 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 2 cut(s) 175, 1047
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 680, 892
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 3 cut(s) 338, 414, 908
BseRI GAGGAG 2 cut(s) 539, 860
BseXI GCAGC 4 cut(s) 472, 568, 913, 988
Bsh1236I CGCG 2 cut(s) 259, 437
BshFI GGCC 4 cut(s) 392, 733, 872, 1190
BshNI GGYRCC 1 cut(s) 683
BsiHKAI GWGCWC 2 cut(s) 648, 1026
BsiHKCI CYCGRG 1 cut(s) 692
BsiSI CCGG 6 cut(s) 133, 393, 886, 918, 939, 1071
BslI CCNNNNNNNGG 2 cut(s) 680, 892
BsmAI GTCTC 4 cut(s) 109, 202, 1105, 1130
BsmBI CGTCTC 1 cut(s) 1105
BsmI GAATGC 1 cut(s) 1241
BsnI GGCC 4 cut(s) 392, 733, 872, 1190
Bso31I GGTCTC 1 cut(s) 1130
BsoBI CYCGRG 1 cut(s) 692
Bsp1286I GDGCHC 2 cut(s) 648, 1026
BspACI CCGC 8 cut(s) 69, 113, 259, 269, 381, 435, 437, 1068
BspANI GGCC 4 cut(s) 392, 733, 872, 1190
BspCNI CTCAG 1 cut(s) 576
BspFNI CGCG 2 cut(s) 259, 437
BspLI GGNNCC 1 cut(s) 685
BspPI GGATC 2 cut(s) 60, 1228
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 1130
BsrI ACTGG 3 cut(s) 338, 414, 908
BssECI CCNNGG 1 cut(s) 435
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 2 cut(s) 457, 956
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 465, 720, 1243, 1247
BstDEI CTNAG 2 cut(s) 560, 584
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 2 cut(s) 175, 1047
BstFNI CGCG 2 cut(s) 259, 437
BstHHI GCGC 1 cut(s) 1241
BstMAI GTCTC 4 cut(s) 109, 202, 1105, 1130
BstMWI GCNNNNNNNGC 6 cut(s) 256, 389, 398, 514, 553, 998
BstSCI CCNGG 1 cut(s) 885
BstSFI CTRYAG 1 cut(s) 474
BstUI CGCG 2 cut(s) 259, 437
BstV1I GCAGC 4 cut(s) 472, 568, 913, 988
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 4 cut(s) 65, 184, 1183, 1196
BstXI CCANNNNNNTGG 1 cut(s) 726
BstYI RGATCY 4 cut(s) 65, 184, 1183, 1196
BsuRI GGCC 4 cut(s) 392, 733, 872, 1190
BtgI CCRYGG 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 249
BtrI CACGTC 1 cut(s) 622
BtsCI GGATG 2 cut(s) 175, 1047
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 4 cut(s) 375, 421, 616, 915
Cac8I GCNNGC 4 cut(s) 465, 720, 1243, 1247
CfoI GCGC 1 cut(s) 1241
Cfr13I GGNCC 1 cut(s) 905
Cfr42I CCGCGG 1 cut(s) 438
Csp6I GTAC 1 cut(s) 850
CviAII CATG 2 cut(s) 265, 688
CviQI GTAC 1 cut(s) 850
DdeI CTNAG 2 cut(s) 560, 584
EaeI YGGCCR 2 cut(s) 390, 1188
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 1057
Eco31I GGTCTC 1 cut(s) 1130
Eco47I GGWCC 1 cut(s) 905
Eco57I CTGAAG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 1105
FaeI CATG 2 cut(s) 268, 691
FatI CATG 2 cut(s) 264, 687
FbaI TGATCA 2 cut(s) 238, 366
Fnu4HI GCNGC 6 cut(s) 260, 381, 486, 557, 927, 1002
FokI GGATG 2 cut(s) 182, 1054
Fsp4HI GCNGC 6 cut(s) 260, 381, 486, 557, 927, 1002
FspBI CTAG 4 cut(s) 677, 794, 1200, 1209
FspI TGCGCA 1 cut(s) 1240
GlaI GCGC 1 cut(s) 1240
GluI GCNGC 6 cut(s) 260, 381, 486, 557, 927, 1002
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 4 cut(s) 392, 733, 872, 1190
HapII CCGG 6 cut(s) 133, 393, 886, 918, 939, 1071
HhaI GCGC 1 cut(s) 1241
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 268, 691
Hin6I GCGC 1 cut(s) 1239
HinP1I GCGC 1 cut(s) 1239
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 2 cut(s) 660, 1165
HinfI GANTC 8 cut(s) 109, 144, 157, 425, 502, 570, 606, 1074
HpaII CCGG 6 cut(s) 133, 393, 886, 918, 939, 1071
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 915, 1012
Hpy188I TCNGA 5 cut(s) 143, 205, 585, 790, 1174
Hpy8I GTNNAC 2 cut(s) 915, 1012
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 2 cut(s) 457, 956
HpyCH4IV ACGT 4 cut(s) 621, 867, 986, 1016
HpyCH4V TGCA 4 cut(s) 54, 250, 304, 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 256, 389, 398, 514, 553, 998
HpyF3I CTNAG 2 cut(s) 560, 584
HpySE526I ACGT 4 cut(s) 621, 867, 986, 1016
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 268, 691
HspAI GCGC 1 cut(s) 1239
Ksp22I TGATCA 2 cut(s) 238, 366
KspI CCGCGG 1 cut(s) 438
LmnI GCTCC 2 cut(s) 526, 790
Lsp1109I GCAGC 4 cut(s) 472, 568, 913, 988
LweI GCATC 2 cut(s) 291, 829
MaeI CTAG 4 cut(s) 677, 794, 1200, 1209
MaeII ACGT 4 cut(s) 621, 867, 986, 1016
MaeIII GTNAC 4 cut(s) 87, 863, 950, 1212
MflI RGATCY 4 cut(s) 65, 184, 1183, 1196
MhlI GDGCHC 2 cut(s) 648, 1026
MlsI TGGCCA 1 cut(s) 1190
MluCI AATT 7 cut(s) 95, 136, 352, 710, 825, 921, 1175
MluNI TGGCCA 1 cut(s) 1190
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 147
Mox20I TGGCCA 1 cut(s) 1190
MroXI GAANNNNTTC 2 cut(s) 156, 424
MscI TGGCCA 1 cut(s) 1190
MseI TTAA 4 cut(s) 99, 828, 881, 1255
Msp20I TGGCCA 1 cut(s) 1190
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 6 cut(s) 133, 393, 886, 918, 939, 1071
MspR9I CCNGG 1 cut(s) 887
Mva1269I GAATGC 1 cut(s) 1241
MvnI CGCG 2 cut(s) 259, 437
MwoI GCNNNNNNNGC 6 cut(s) 256, 389, 398, 514, 553, 998
NciI CCSGG 1 cut(s) 887
NlaIII CATG 2 cut(s) 268, 691
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 1 cut(s) 950
NsbI TGCGCA 1 cut(s) 1240
PaeR7I CTCGAG 1 cut(s) 692
PctI GAATGC 1 cut(s) 1241
PdmI GAANNNNTTC 2 cut(s) 156, 424
PfeI GAWTC 7 cut(s) 109, 157, 425, 502, 570, 606, 1074
PkrI GCNGC 6 cut(s) 261, 382, 487, 558, 928, 1003
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 1 cut(s) 905
PsuI RGATCY 4 cut(s) 65, 184, 1183, 1196
RsaI GTAC 1 cut(s) 851
RsaNI GTAC 1 cut(s) 850
SacII CCGCGG 1 cut(s) 438
SaqAI TTAA 4 cut(s) 99, 828, 881, 1255
SatI GCNGC 6 cut(s) 260, 381, 486, 557, 927, 1002
Sau96I GGNCC 1 cut(s) 905
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 1 cut(s) 887
SduI GDGCHC 2 cut(s) 648, 1026
SfaNI GCATC 2 cut(s) 291, 829
SfcI CTRYAG 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 692
Sfr303I CCGCGG 1 cut(s) 438
SgrBI CCGCGG 1 cut(s) 438
SinI GGWCC 1 cut(s) 905
SlaI CTCGAG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 692, 705
SmoI CTYRAG 2 cut(s) 692, 705
Sse9I AATT 7 cut(s) 95, 136, 352, 710, 825, 921, 1175
SsiI CCGC 8 cut(s) 69, 113, 259, 269, 381, 435, 437, 1068
SspMI CTAG 4 cut(s) 677, 794, 1200, 1209
StyD4I CCNGG 1 cut(s) 885
TaaI ACNGT 2 cut(s) 457, 956
TaiI ACGT 4 cut(s) 624, 870, 989, 1019
TaqI TCGA 5 cut(s) 107, 147, 573, 693, 1134
TasI AATT 7 cut(s) 95, 136, 352, 710, 825, 921, 1175
TauI GCSGC 2 cut(s) 262, 383
TfiI GAWTC 7 cut(s) 109, 157, 425, 502, 570, 606, 1074
Tru1I TTAA 4 cut(s) 99, 828, 881, 1255
Tru9I TTAA 4 cut(s) 99, 828, 881, 1255
TscAI CASTG 4 cut(s) 382, 421, 616, 915
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 4 cut(s) 485, 556, 926, 1001
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 4 cut(s) 422, 509, 984, 1248
TspGWI ACGGA 4 cut(s) 346, 418, 782, 1041
TspRI CASTG 4 cut(s) 382, 421, 616, 915
VpaK11BI GGWCC 1 cut(s) 905
XapI RAATTY 3 cut(s) 95, 136, 710
XcmI CCANNNNNNNNNTGG 1 cut(s) 726
XhoI CTCGAG 1 cut(s) 692
XmnI GAANNNNTTC 2 cut(s) 156, 424
XspI CTAG 4 cut(s) 677, 794, 1200, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.