pycom05g31680

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009170
Physical Location & Seq
Reverse (-)
31323763 .. 31325052
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31680.1

Sequence Viewer

Length: 1290 bp
ATGGCTTCCCTCCAAACTTCATCTCCTGTCTTCTCTTCTCCCTCACGTCGTTCTTCCTCAAACAGAACCATAAATGCCGCCATTCATGCCCGCAAGCTCCCAAGAGTCCGCTTCGCTGACCTGAAACAAACCACGAAGCTCGTCCTCCAAGGTCTCCAGTTGAAGGAACAAGTTCTGCAACAGGAAAACACCGATTTGTATAGCAACAATCACTCTGCAGCAGCAGCAGCAACAGCAGCAGCAGCTCCTACAGCTTCCACAACTCAGCTCTACGCGCTCTTAGAGGCTGTGGTGGACAGAGTGGAGATGCACGCGAACATCGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCGATCTCTCCGCTTCCCTAATGGCCGGAGTTAATGCAGCTGCTGGAACGTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCTACCGGAATGCTGCATATCATGAACAAGATCCAGCCTTCGCAGCTCGCTGAGGAGCAGCGCAATGCTACGAGGTTGTTCAGGCAGCTCCAGAGCAAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGGTTTTGGCTCTTGACAAAGCTTACCCTCTTGCGTTGCTCGGTGCCATGCTCGAAAAATTCCCCAAAAAATTGGAGCCGACTGTTTGGTGGCCTAGAAATGTGGTTCAATCTCAAAATCCAAGGAAACCGAAATCCCAGATGGGAAATAATGTGAATGGATGGAGTGAAGAACTTGAAAGGGAAATGAGAGAAATTATTCAAGTTGTGAAGAACAAGGACCTTGAAAACTATGTGAGATTAGGAAGCTCGGTAGTATTAAAGACCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGGGATCGATCCTGTTGGATCCGCTTTTGTAGGCCATGGATCAGCCACTGTCGCGGTTGCAACAGTGGCTGCAGGCTCTTTGGCTAGCGCAGTGAATGCTTTTCAGCATGGCGGGCAAGTAGGGATGGTGTTTGAGATGAATAGGAACTGCGCCGGCTTCTTCAACAAGTTGGAGGATTCAATTGAAGCAACACTTGGAGAACAAGACTGGGAGAAGAGAGAAAATGGGGTGGTTTTTGAAATGAAGGTTGCAATGCGGTTGGGAAGAAGCTTGGAACAGCTCAGAGAACTTGCATCGAAATCAGCTTTGTATCGTTCGAACGGAGAACCCATAGACGAATTCGGTTGCAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

430

Amino Acids

46.85

Weight (kDa)

9.05

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 89 - 405 1.3e-145 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 671
AccII CGCG 3 cut(s) 275, 314, 983
AciI CCGC 9 cut(s) 78, 91, 109, 384, 465, 951, 983, 1041, 1186
AclWI GGATC 7 cut(s) 493, 587, 932, 941, 942, 955, 976
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 686, 1268
AfiI CCNNNNNNNGG 1 cut(s) 163
AjiI CACGTC 1 cut(s) 47
AjuI GAANNNNNNNTTGG 2 cut(s) 1107, 1139
Alw26I GTCTC 3 cut(s) 158, 566, 611
AlwI GGATC 7 cut(s) 493, 587, 932, 941, 942, 955, 976
AlwNI CAGNNNCTG 3 cut(s) 416, 977, 998
AoxI GGCC 4 cut(s) 396, 719, 916, 961
ApaI GGGCCC 1 cut(s) 920
ApoI RAATTY 2 cut(s) 686, 1268
Asp700I GAANNNNTTC 2 cut(s) 171, 825
AspLEI GCGC 5 cut(s) 277, 333, 531, 1019, 1082
AspS9I GGNCC 3 cut(s) 847, 916, 917
AsuII TTCGAA 1 cut(s) 1247
AsuNHI GCTAGC 1 cut(s) 1013
AvaII GGWCC 1 cut(s) 847
BaeGI GKGCMC 1 cut(s) 920
BamHI GGATCC 1 cut(s) 947
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 1 cut(s) 920
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 519
BccI CCATC 3 cut(s) 763, 783, 1048
BcoDI GTCTC 3 cut(s) 158, 566, 611
BfaI CTAG 2 cut(s) 723, 1014
BfmI CTRYAG 3 cut(s) 216, 249, 999
Bme18I GGWCC 1 cut(s) 847
BmgBI CACGTC 1 cut(s) 47
BmgT120I GGNCC 3 cut(s) 847, 916, 917
BmiI GGNNCC 4 cut(s) 673, 705, 918, 949
BmrI ACTGGG 2 cut(s) 939, 1147
BmsI GCATC 2 cut(s) 297, 1232
BmtI GCTAGC 1 cut(s) 1017
BmuI ACTGGG 2 cut(s) 939, 1147
BpiI GAAGAC 1 cut(s) 22
BpmI CTGGAG 2 cut(s) 140, 542
Bpu10I CCTNAGC 1 cut(s) 519
Bpu14I TTCGAA 1 cut(s) 1247
Bsa29I ATCGAT 2 cut(s) 375, 936
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 3 cut(s) 148, 749, 964
BsaWI WCCGGW 1 cut(s) 473
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 4 cut(s) 157, 344, 934, 1142
Bse3DI GCAATG 2 cut(s) 538, 1188
BseCI ATCGAT 2 cut(s) 375, 936
BseDI CCNNGG 3 cut(s) 148, 749, 964
BseGI GGATG 2 cut(s) 794, 1059
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMI GCAATG 2 cut(s) 538, 1188
BseMII CTCAG 4 cut(s) 278, 510, 591, 1225
BseNI ACTGG 4 cut(s) 157, 344, 934, 1142
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 920
Bsh1236I CGCG 3 cut(s) 275, 314, 983
BshFI GGCC 4 cut(s) 398, 721, 918, 963
BshNI GGYRCC 1 cut(s) 671
BshVI ATCGAT 2 cut(s) 375, 936
BsiSI CCGG 3 cut(s) 399, 474, 1083
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 3 cut(s) 158, 566, 611
BsmBI CGTCTC 1 cut(s) 566
BsmI GAATGC 2 cut(s) 483, 1030
BsnI GGCC 4 cut(s) 398, 721, 918, 963
Bso31I GGTCTC 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 1247
Bsp120I GGGCCC 1 cut(s) 916
Bsp1286I GDGCHC 1 cut(s) 920
Bsp143I GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
Bsp19I CCATGG 1 cut(s) 964
BspACI CCGC 9 cut(s) 78, 91, 109, 384, 465, 951, 983, 1041, 1186
BspANI GGCC 4 cut(s) 398, 721, 918, 963
BspCNI CTCAG 4 cut(s) 277, 511, 592, 1224
BspDI ATCGAT 2 cut(s) 375, 936
BspFNI CGCG 3 cut(s) 275, 314, 983
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 4 cut(s) 673, 705, 918, 949
BspMAI CTGCAG 2 cut(s) 220, 1003
BspOI GCTAGC 1 cut(s) 1017
BspPI GGATC 7 cut(s) 493, 587, 932, 941, 942, 955, 976
BspQI GCTCTTC 1 cut(s) 591
BspT104I TTCGAA 1 cut(s) 1247
BspT107I GGYRCC 1 cut(s) 671
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 538, 1188
BsrFI RCCGGY 1 cut(s) 1082
BsrI ACTGG 4 cut(s) 157, 344, 934, 1142
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 3 cut(s) 148, 749, 964
BssMI GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
BssT1I CCWWGG 3 cut(s) 148, 749, 964
Bst4CI ACNGT 4 cut(s) 577, 712, 979, 994
Bst6I CTCTTC 3 cut(s) 40, 591, 1139
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstBI TTCGAA 1 cut(s) 1247
BstDEI CTNAG 6 cut(s) 264, 280, 356, 519, 600, 1211
BstDSI CCRYGG 1 cut(s) 964
BstF5I GGATG 2 cut(s) 794, 1059
BstFNI CGCG 3 cut(s) 275, 314, 983
BstHHI GCGC 5 cut(s) 277, 333, 531, 1019, 1082
BstKTI GATC 8 cut(s) 375, 379, 501, 595, 936, 940, 950, 971
BstMAI GTCTC 3 cut(s) 158, 566, 611
BstMBI GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
BstSFI CTRYAG 3 cut(s) 216, 249, 999
BstSLI GKGCMC 1 cut(s) 920
BstUI CGCG 3 cut(s) 275, 314, 983
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 498, 947
BstXI CCANNNNNNTGG 1 cut(s) 700
BstYI RGATCY 2 cut(s) 498, 947
Bsu15I ATCGAT 2 cut(s) 375, 936
BsuRI GGCC 4 cut(s) 398, 721, 918, 963
BsuTUI ATCGAT 2 cut(s) 375, 936
BtgI CCRYGG 1 cut(s) 964
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 2 cut(s) 794, 1059
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 3 cut(s) 975, 999, 1026
CaiI CAGNNNCTG 3 cut(s) 416, 977, 998
CciI TCATGA 1 cut(s) 489
CfoI GCGC 5 cut(s) 277, 333, 531, 1019, 1082
Cfr10I RCCGGY 1 cut(s) 1082
Cfr13I GGNCC 3 cut(s) 847, 916, 917
ClaI ATCGAT 2 cut(s) 375, 936
CviAII CATG 6 cut(s) 86, 370, 490, 676, 965, 1037
DdeI CTNAG 6 cut(s) 264, 280, 356, 519, 600, 1211
DpnI GATC 8 cut(s) 374, 378, 500, 594, 935, 939, 949, 970
DpnII GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 40, 591, 1139
EarI CTCTTC 3 cut(s) 40, 591, 1139
Eco130I CCWWGG 3 cut(s) 148, 749, 964
Eco24I GRGCYC 1 cut(s) 920
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 847
EcoO109I RGGNCCY 2 cut(s) 847, 917
EcoRI GAATTC 1 cut(s) 1268
EcoT14I CCWWGG 3 cut(s) 148, 749, 964
EcoT38I GRGCYC 1 cut(s) 920
ErhI CCWWGG 3 cut(s) 148, 749, 964
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 6 cut(s) 89, 373, 493, 679, 968, 1040
FalI AAGNNNNNCTT 2 cut(s) 1107, 1139
FatI CATG 6 cut(s) 85, 369, 489, 675, 964, 1036
FauI CCCGC 2 cut(s) 98, 1034
FokI GGATG 2 cut(s) 801, 1066
FriOI GRGCYC 1 cut(s) 920
FspBI CTAG 2 cut(s) 723, 1014
GlaI GCGC 5 cut(s) 276, 332, 530, 1018, 1081
GsuI CTGGAG 2 cut(s) 140, 542
HaeIII GGCC 4 cut(s) 398, 721, 918, 963
HapII CCGG 3 cut(s) 399, 474, 1083
HhaI GCGC 5 cut(s) 277, 333, 531, 1019, 1082
Hin1II CATG 6 cut(s) 89, 373, 493, 679, 968, 1040
Hin6I GCGC 5 cut(s) 275, 331, 529, 1017, 1080
HinP1I GCGC 5 cut(s) 275, 331, 529, 1017, 1080
HindIII AAGCTT 3 cut(s) 648, 1198, 1280
HinfI GANTC 2 cut(s) 105, 1106
HpaII CCGG 3 cut(s) 399, 474, 1083
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 1 cut(s) 1214
Hpy188III TCNNGA 4 cut(s) 490, 559, 590, 641
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 5 cut(s) 157, 516, 607, 622, 1168
HpyCH4III ACNGT 4 cut(s) 577, 712, 979, 994
HpyCH4IV ACGT 2 cut(s) 46, 422
HpyF3I CTNAG 6 cut(s) 264, 280, 356, 519, 600, 1211
HpySE526I ACGT 2 cut(s) 46, 422
Hsp92II CATG 6 cut(s) 89, 373, 493, 679, 968, 1040
HspAI GCGC 5 cut(s) 275, 331, 529, 1017, 1080
KroI GCCGGC 1 cut(s) 1082
KroNI GCCGGC 1 cut(s) 1084
Kzo9I GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 5 cut(s) 102, 250, 523, 561, 703
LweI GCATC 2 cut(s) 297, 1232
MaeI CTAG 2 cut(s) 723, 1014
MaeII ACGT 2 cut(s) 46, 422
MalI GATC 8 cut(s) 374, 378, 500, 594, 935, 939, 949, 970
MboI GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
MboII GAAGA 9 cut(s) 22, 27, 45, 578, 809, 850, 1081, 1156, 1206
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 2 cut(s) 498, 947
MhlI GDGCHC 1 cut(s) 920
MluCI AATT 6 cut(s) 567, 686, 698, 822, 1110, 1268
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 925, 1080
MnlI CCTC 9 cut(s) 20, 52, 67, 155, 277, 514, 534, 666, 1096
MroNI GCCGGC 1 cut(s) 1082
MroXI GAANNNNTTC 2 cut(s) 171, 825
MseI TTAA 2 cut(s) 405, 887
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 3 cut(s) 399, 474, 1083
MunI CAATTG 1 cut(s) 1110
Mva1269I GAATGC 2 cut(s) 483, 1030
MvnI CGCG 3 cut(s) 275, 314, 983
NaeI GCCGGC 1 cut(s) 1084
NcoI CCATGG 1 cut(s) 964
NdeII GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
NgoMIV GCCGGC 1 cut(s) 1082
NheI GCTAGC 1 cut(s) 1013
NlaIII CATG 6 cut(s) 89, 373, 493, 679, 968, 1040
NlaIV GGNNCC 4 cut(s) 673, 705, 918, 949
NspV TTCGAA 1 cut(s) 1247
PagI TCATGA 1 cut(s) 489
PciSI GCTCTTC 1 cut(s) 591
PctI GAATGC 2 cut(s) 483, 1030
PdiI GCCGGC 1 cut(s) 1084
PdmI GAANNNNTTC 2 cut(s) 171, 825
PfeI GAWTC 1 cut(s) 1106
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 847
PspN4I GGNNCC 4 cut(s) 673, 705, 918, 949
PspOMI GGGCCC 1 cut(s) 916
PspPI GGNCC 3 cut(s) 847, 916, 917
PspPPI RGGWCCY 1 cut(s) 847
PstI CTGCAG 2 cut(s) 220, 1003
PstNI CAGNNNCTG 3 cut(s) 416, 977, 998
PsuI RGATCY 2 cut(s) 498, 947
PvuII CAGCTG 1 cut(s) 413
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 2 cut(s) 405, 887
Sau3AI GATC 8 cut(s) 372, 376, 498, 592, 933, 937, 947, 968
Sau96I GGNCC 3 cut(s) 847, 916, 917
SchI GAGTC 1 cut(s) 114
SduI GDGCHC 1 cut(s) 920
SfaNI GCATC 2 cut(s) 297, 1232
SfcI CTRYAG 3 cut(s) 216, 249, 999
SfuI TTCGAA 1 cut(s) 1247
SinI GGWCC 1 cut(s) 847
Sse9I AATT 6 cut(s) 567, 686, 698, 822, 1110, 1268
SsiI CCGC 9 cut(s) 78, 91, 109, 384, 465, 951, 983, 1041, 1186
SspMI CTAG 2 cut(s) 723, 1014
StyI CCWWGG 3 cut(s) 148, 749, 964
TaaI ACNGT 4 cut(s) 577, 712, 979, 994
TaiI ACGT 2 cut(s) 49, 425
TaqI TCGA 5 cut(s) 375, 681, 936, 1226, 1247
TasI AATT 6 cut(s) 567, 686, 698, 822, 1110, 1268
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 1106
Tru1I TTAA 2 cut(s) 405, 887
Tru9I TTAA 2 cut(s) 405, 887
TscAI CASTG 3 cut(s) 982, 999, 1026
TspDTI ATGAA 6 cut(s) 9, 74, 435, 506, 1082, 1187
TspGWI ACGGA 2 cut(s) 415, 1266
TspRI CASTG 3 cut(s) 982, 999, 1026
VpaK11BI GGWCC 1 cut(s) 847
XapI RAATTY 2 cut(s) 686, 1268
XcmI CCANNNNNNNNNTGG 1 cut(s) 901
XmnI GAANNNNTTC 2 cut(s) 171, 825
XspI CTAG 2 cut(s) 723, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.