MD05G1348900.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009170
Physical Location & Seq
Reverse (-)
46704498 .. 46705475
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1348900.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCTTCCCTCCAAACTTCATCTCTTGTCTCACGTTGTTCTTCCTCAAACAGAACCATAAATGCCGCCATTCATGTCCGCACGCTCCCAAGGGTCCGCTTCGCTGACCCAAAACAAACCACGAAGCTCGTCCTCGAAGGCCTCCAGTTGAAGGAACAAGTTCTGAAACACGAAAACACCGATTTGTATAACAACAATCACTCTGCAGCAGCAGCAGCCGCGCCTACAGCTTCCACAACTCAGCTCTACGCGCTCTTAGAGGCGGTGGCGGACAGAGTGGAGATGCACGCAAACATCGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCACTCTCTCCGCTTCCCTAATGGCCGGAGTTAATGCAGCTGCTGGAACGTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCTACCGGAATGCTGCTTATCATGAACAAGATCCAGCCTTCGCAGCTCGCCGAGGAGCAGCGCAATGCTACGAGGTTGTTCAGGCAGCTCCAGAGCGAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGGTTTTGGCTCTTGACAAAGCTTACCCACTTCCGTTGCTCGGTGCCATGCTCGAAAAGTTCCCCAAAAAAATTGAGCCGGCTGTTTGGTGGCCTAGAAATGTTGTTCAATCTCAAAATCCAAGGAAACCGAAATCCCAGATGGGAAATAATGTGAATGGATGGAGTGAAGAACTTGAAAGCGAAATGAGAGAAATTATTCAAGTTGTGAAGAACAAGGACATTGAAGACTATGAGAGATTAGGAAGCTTGGCGTTAAAGACCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGCGATCGCGGCTGTTGGATCTGCTTTTGTAGGCCATGGATCAGCCAATCAGCCACTGCCAGCCGGTTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

326

Amino Acids

35.35

Weight (kDa)

8.78

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 81 - 316 1.5e-113 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 647
AccII CGCG 3 cut(s) 221, 251, 911
AciI CCGC 9 cut(s) 66, 79, 97, 219, 263, 269, 360, 441, 911
AclWI GGATC 4 cut(s) 469, 563, 928, 949
AcoI YGGCCR 1 cut(s) 372
AfiI CCNNNNNNNGG 2 cut(s) 151, 644
AgsI TTSAA 6 cut(s) 151, 415, 713, 782, 806, 830
Alw26I GTCTC 3 cut(s) 34, 542, 587
AlwI GGATC 4 cut(s) 469, 563, 928, 949
AlwNI CAGNNNCTG 2 cut(s) 392, 958
AoxI GGCC 5 cut(s) 139, 372, 695, 889, 934
ApaI GGGCCC 1 cut(s) 893
AsiSI GCGATCGC 1 cut(s) 909
Asp700I GAANNNNTTC 2 cut(s) 159, 801
AspLEI GCGC 4 cut(s) 223, 253, 309, 507
AspS9I GGNCC 3 cut(s) 94, 889, 890
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 893
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 893
BbsI GAAGAC 1 cut(s) 837
BccI CCATC 2 cut(s) 739, 759
BclI TGATCA 1 cut(s) 348
BcoDI GTCTC 3 cut(s) 34, 542, 587
BfaI CTAG 2 cut(s) 699, 976
BfmI CTRYAG 2 cut(s) 204, 225
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 3 cut(s) 94, 889, 890
BmiI GGNNCC 3 cut(s) 95, 649, 891
BmsI GCATC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 2 cut(s) 128, 518
BsaJI CCNNGG 4 cut(s) 89, 495, 725, 937
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 644
Bse118I RCCGGY 2 cut(s) 682, 965
Bse1I ACTGG 2 cut(s) 145, 320
Bse3DI GCAATG 1 cut(s) 514
BseDI CCNNGG 4 cut(s) 89, 495, 725, 937
BseGI GGATG 1 cut(s) 770
BseLI CCNNNNNNNGG 2 cut(s) 151, 644
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 2 cut(s) 254, 567
BseNI ACTGG 2 cut(s) 145, 320
BseRI GAGGAG 1 cut(s) 512
BseSI GKGCMC 1 cut(s) 893
Bsh1236I CGCG 3 cut(s) 221, 251, 911
Bsh1285I CGRYCG 1 cut(s) 909
BshFI GGCC 5 cut(s) 141, 374, 697, 891, 936
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 909
BsiSI CCGG 4 cut(s) 375, 450, 683, 966
BslI CCNNNNNNNGG 2 cut(s) 151, 644
BsmAI GTCTC 3 cut(s) 34, 542, 587
BsmBI CGTCTC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 5 cut(s) 141, 374, 697, 891, 936
Bsp120I GGGCCC 1 cut(s) 889
Bsp1286I GDGCHC 1 cut(s) 893
Bsp143I GATC 6 cut(s) 348, 474, 568, 906, 920, 941
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 9 cut(s) 66, 79, 97, 219, 263, 269, 360, 441, 911
BspANI GGCC 5 cut(s) 141, 374, 697, 891, 936
BspCNI CTCAG 2 cut(s) 253, 568
BspFNI CGCG 3 cut(s) 221, 251, 911
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 3 cut(s) 95, 649, 891
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 4 cut(s) 469, 563, 928, 949
BspQI GCTCTTC 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 2 cut(s) 682, 965
BsrI ACTGG 2 cut(s) 145, 320
BssAI RCCGGY 2 cut(s) 682, 965
BssECI CCNNGG 4 cut(s) 89, 495, 725, 937
BssMI GATC 6 cut(s) 348, 474, 568, 906, 920, 941
BssT1I CCWWGG 3 cut(s) 89, 725, 937
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 1 cut(s) 567
BstC8I GCNNGC 6 cut(s) 83, 288, 302, 492, 684, 963
BstDEI CTNAG 4 cut(s) 240, 256, 332, 576
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 1 cut(s) 770
BstFNI CGCG 3 cut(s) 221, 251, 911
BstHHI GCGC 4 cut(s) 223, 253, 309, 507
BstKTI GATC 6 cut(s) 351, 477, 571, 909, 923, 944
BstMAI GTCTC 3 cut(s) 34, 542, 587
BstMBI GATC 6 cut(s) 348, 474, 568, 906, 920, 941
BstMCI CGRYCG 1 cut(s) 909
BstMWI GCNNNNNNNGC 8 cut(s) 212, 215, 218, 227, 250, 306, 487, 911
BstSFI CTRYAG 2 cut(s) 204, 225
BstSLI GKGCMC 1 cut(s) 893
BstUI CGCG 3 cut(s) 221, 251, 911
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 2 cut(s) 474, 920
BstYI RGATCY 2 cut(s) 474, 920
BsuRI GGCC 5 cut(s) 141, 374, 697, 891, 936
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 1 cut(s) 770
BtsI GCAGTG 1 cut(s) 956
BtsIMutI CAGTG 1 cut(s) 956
Cac8I GCNNGC 6 cut(s) 83, 288, 302, 492, 684, 963
CaiI CAGNNNCTG 2 cut(s) 392, 958
CciI TCATGA 1 cut(s) 465
CfoI GCGC 4 cut(s) 223, 253, 309, 507
Cfr10I RCCGGY 2 cut(s) 682, 965
Cfr13I GGNCC 3 cut(s) 94, 889, 890
CspCI CAANNNNNGTGG 2 cut(s) 621, 656
CviAII CATG 5 cut(s) 74, 346, 466, 652, 938
DdeI CTNAG 4 cut(s) 240, 256, 332, 576
DpnI GATC 6 cut(s) 350, 476, 570, 908, 922, 943
DpnII GATC 6 cut(s) 348, 474, 568, 906, 920, 941
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
EciI GGCGGA 1 cut(s) 284
Eco130I CCWWGG 3 cut(s) 89, 725, 937
Eco147I AGGCCT 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 893
Eco47I GGWCC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 890
EcoT14I CCWWGG 3 cut(s) 89, 725, 937
EcoT38I GRGCYC 1 cut(s) 893
ErhI CCWWGG 3 cut(s) 89, 725, 937
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 5 cut(s) 77, 349, 469, 655, 941
FaiI YATR 8 cut(s) 59, 75, 189, 347, 467, 653, 837, 939
FatI CATG 5 cut(s) 73, 345, 465, 651, 937
FbaI TGATCA 1 cut(s) 348
FokI GGATG 1 cut(s) 777
FriOI GRGCYC 1 cut(s) 893
FspBI CTAG 2 cut(s) 699, 976
GlaI GCGC 4 cut(s) 222, 252, 308, 506
GsuI CTGGAG 2 cut(s) 128, 518
HaeIII GGCC 5 cut(s) 141, 374, 697, 891, 936
HapII CCGG 4 cut(s) 375, 450, 683, 966
HhaI GCGC 4 cut(s) 223, 253, 309, 507
Hin1II CATG 5 cut(s) 77, 349, 469, 655, 941
Hin6I GCGC 4 cut(s) 221, 251, 307, 505
HinP1I GCGC 4 cut(s) 221, 251, 307, 505
HindIII AAGCTT 2 cut(s) 624, 850
HpaII CCGG 4 cut(s) 375, 450, 683, 966
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 4 cut(s) 466, 535, 566, 617
HpyAV CCTTC 5 cut(s) 131, 145, 492, 583, 598
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 2 cut(s) 34, 398
HpyCH4V TGCA 4 cut(s) 206, 286, 386, 972
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 215, 218, 227, 250, 306, 487, 911
HpyF3I CTNAG 4 cut(s) 240, 256, 332, 576
HpySE526I ACGT 2 cut(s) 34, 398
Hsp92II CATG 5 cut(s) 77, 349, 469, 655, 941
HspAI GCGC 4 cut(s) 221, 251, 307, 505
KroI GCCGGC 1 cut(s) 682
KroNI GCCGGC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 6 cut(s) 348, 474, 568, 906, 920, 941
LguI GCTCTTC 1 cut(s) 567
LmnI GCTCC 3 cut(s) 90, 499, 537
LweI GCATC 1 cut(s) 273
MaeI CTAG 2 cut(s) 699, 976
MaeII ACGT 2 cut(s) 34, 398
MalI GATC 6 cut(s) 350, 476, 570, 908, 922, 943
MboI GATC 6 cut(s) 348, 474, 568, 906, 920, 941
MboII GAAGA 5 cut(s) 33, 554, 785, 826, 842
MflI RGATCY 2 cut(s) 474, 920
MhlI GDGCHC 1 cut(s) 893
MluCI AATT 3 cut(s) 543, 675, 798
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 7 cut(s) 20, 55, 143, 152, 253, 490, 510
MroNI GCCGGC 1 cut(s) 682
MroXI GAANNNNTTC 2 cut(s) 159, 801
MseI TTAA 2 cut(s) 381, 860
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 4 cut(s) 375, 450, 683, 966
Mva1269I GAATGC 1 cut(s) 459
MvnI CGCG 3 cut(s) 221, 251, 911
MwoI GCNNNNNNNGC 8 cut(s) 212, 215, 218, 227, 250, 306, 487, 911
NaeI GCCGGC 1 cut(s) 684
NcoI CCATGG 1 cut(s) 937
NdeII GATC 6 cut(s) 348, 474, 568, 906, 920, 941
NgoMIV GCCGGC 1 cut(s) 682
NlaIII CATG 5 cut(s) 77, 349, 469, 655, 941
NlaIV GGNNCC 3 cut(s) 95, 649, 891
NmeAIII GCCGAG 1 cut(s) 520
PagI TCATGA 1 cut(s) 465
PceI AGGCCT 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 567
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 684
PdmI GAANNNNTTC 2 cut(s) 159, 801
Ple19I CGATCG 1 cut(s) 909
PspN4I GGNNCC 3 cut(s) 95, 649, 891
PspOMI GGGCCC 1 cut(s) 889
PspPI GGNCC 3 cut(s) 94, 889, 890
PstI CTGCAG 1 cut(s) 208
PstNI CAGNNNCTG 2 cut(s) 392, 958
PsuI RGATCY 2 cut(s) 474, 920
PvuI CGATCG 1 cut(s) 909
PvuII CAGCTG 1 cut(s) 389
RgaI GCGATCGC 1 cut(s) 909
SapI GCTCTTC 1 cut(s) 567
SaqAI TTAA 2 cut(s) 381, 860
Sau3AI GATC 6 cut(s) 348, 474, 568, 906, 920, 941
Sau96I GGNCC 3 cut(s) 94, 889, 890
SduI GDGCHC 1 cut(s) 893
SfaAI GCGATCGC 1 cut(s) 909
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 2 cut(s) 204, 225
SgfI GCGATCGC 1 cut(s) 909
SinI GGWCC 1 cut(s) 94
Sse9I AATT 3 cut(s) 543, 675, 798
SseBI AGGCCT 1 cut(s) 141
SsiI CCGC 9 cut(s) 66, 79, 97, 219, 263, 269, 360, 441, 911
SspMI CTAG 2 cut(s) 699, 976
StuI AGGCCT 1 cut(s) 141
StyI CCWWGG 3 cut(s) 89, 725, 937
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 2 cut(s) 37, 401
TaqI TCGA 2 cut(s) 135, 657
TasI AATT 3 cut(s) 543, 675, 798
TauI GCSGC 3 cut(s) 68, 221, 914
Tru1I TTAA 2 cut(s) 381, 860
Tru9I TTAA 2 cut(s) 381, 860
TscAI CASTG 1 cut(s) 963
TspDTI ATGAA 4 cut(s) 9, 62, 411, 482
TspGWI ACGGA 2 cut(s) 391, 627
TspRI CASTG 1 cut(s) 963
VpaK11BI GGWCC 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XmnI GAANNNNTTC 2 cut(s) 159, 801
XspI CTAG 2 cut(s) 699, 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.