Rh5DG022500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009170
Physical Location & Seq
Forward (+)
1601741 .. 1603487
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG022500.1

Sequence Viewer

Length: 1287 bp
ATGGCTTGTTTGCAAACTTCAAGTCTCTTATTCCCCACATCTTCAACTCTTTCTAGTTCCTCAAGGAGAATGATCAATGCTGCAATCCAAGTCCCCAAAGTTCCAAGACTCCGCTTCACCAACCCGAAAACCGCAACCAAGTTGGTACTTGAAGGGCTACAATTGAGTGATGGGATCTCAAACACGTACCAAATAGCTCAACCAAAGCATCAAGAATCATCAAAGCACTTTCATTCTACTACAACTAGTACTTCTACAGCTACCATTCAGCTCTATGCAATCCTAGAGGAAGTAGCAGATAGAATTGAGATGCACATCAATATCGGCGAGCAGCGCAACAACTGGAACGTTCTTCTGCTCAACTCCATCAACATGATCACTCTCACAGCCTCAATCATGGCTGTAATTGCAGGCACTGTTGGTGCTGGAATGCCCATTTTGGCCTTGAAGTTGTCTTCCGCTCTTTTATTTACTGCAGCCTCAGGAATGTTGCTTATCATGAACAAGATCCAACCTTCACAACTAGCTGAAGAACAGCGCAATGCCACAAGATTGTTCAAGCAGATCCAAACTCAAATCCAAACCTTACTAGCACTTCGTGAGCCGACAGAACAAGATGTGAAGGAAACAATGGAGAAAGTTTTAGCTCTTGACAAAGCGTACCCACTTCCATTGCTTGGAGCTATGCTTGAAAAGTGCCCTAAAAAGTTTGAGGCAGCTGCTTGGTGGCCTAAAAGTCATGGAGTTCAAAATCCAAGCGAAACGAAATCTAGGATGGGAAAGACTAATGGGTGGAGTGAAGATCTTGAAGATGAAATGAGAGAAATTATTTCAGTGGTGAAAAGCAAAGACTTCGAAGACTACGAGAGATTAGGTAACTTGGCACTGAAGATCAACAAGGTTTTGGCTATTTCAGGTCCTTTGCTTACTGGTGTTGCAGCCATTGGGTCTGCTTTTGTGGGTCACAATGATGTTGCAGCCATTGCTGCAGTGGCTGCCGGTTCATTGAGCACTGCAGTCAATGTTTTTCAACATGGTGGTCAAGTTGGGATGGTGTTTGAGATGTATAGGAACTGTGCTGGCTTCTTCAACAAATTGGAGGATACAATTGAAGCCACACTTGGAGAGCAAGATTTGGAGAAAAGAGAAAATGGTGAATTGTTTGAGATGAAGATTGCTTTGGAATTGGGAAGAAGCCTGCCTCAGCTTAGAGAACTTGCATCAAAATCAGCTTCATGTAGGAGAAATGGAACAGTTATTGACGAAATGGGTTCGAAACTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

428

Amino Acids

46.85

Weight (kDa)

7.07

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 90 - 404 1.8e-153 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 677
AccBSI CCGCTC 1 cut(s) 461
AciI CCGC 3 cut(s) 112, 132, 459
AclI AACGTT 1 cut(s) 348
AclWI GGATC 3 cut(s) 182, 502, 559
AcuI CTGAAG 2 cut(s) 549, 908
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 4 cut(s) 147, 188, 250, 662
AfiI CCNNNNNNNGG 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 183
AhlI ACTAGT 1 cut(s) 245
AjuI GAANNNNNNNTTGG 4 cut(s) 1104, 1136, 1163, 1195
AluBI AGCT 9 cut(s) 197, 260, 271, 527, 647, 683, 719, 1207, 1232
AluI AGCT 9 cut(s) 197, 260, 271, 527, 647, 683, 719, 1207, 1232
Alw21I GWGCWC 1 cut(s) 1013
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 3 cut(s) 182, 502, 559
AlwNI CAGNNNCTG 2 cut(s) 416, 995
AoxI GGCC 2 cut(s) 441, 728
ApeKI GCWGC 9 cut(s) 80, 331, 476, 716, 719, 938, 977, 986, 995
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
Asp700I GAANNNNTTC 1 cut(s) 1280
AspLEI GCGC 2 cut(s) 336, 540
AspS9I GGNCC 1 cut(s) 917
AsuHPI GGTGA 3 cut(s) 109, 850, 1166
AsuII TTCGAA 2 cut(s) 855, 1274
AvaII GGWCC 1 cut(s) 917
AxyI CCTNAGG 1 cut(s) 481
BaeGI GKGCMC 1 cut(s) 701
BbsI GAAGAC 2 cut(s) 447, 864
Bbv12I GWGCWC 1 cut(s) 1013
BbvCI CCTCAGC 1 cut(s) 1203
BbvI GCAGC 9 cut(s) 67, 343, 488, 706, 728, 950, 973, 982, 989
BccI CCATC 4 cut(s) 164, 374, 769, 1045
BcgI CGANNNNNNTGC 2 cut(s) 835, 869
BciVI GTATCC 1 cut(s) 1096
BclI TGATCA 2 cut(s) 72, 375
BcoDI GTCTC 1 cut(s) 29
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 6 cut(s) 54, 246, 284, 524, 590, 771
BfmI CTRYAG 4 cut(s) 255, 474, 987, 1014
BfuI GTATCC 1 cut(s) 1096
BglII AGATCT 1 cut(s) 802
BisI GCNGC 9 cut(s) 81, 332, 477, 717, 720, 939, 978, 987, 996
BlsI GCNGC 9 cut(s) 82, 333, 478, 718, 721, 940, 979, 988, 997
BmcAI AGTACT 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 917
BmgT120I GGNCC 1 cut(s) 917
BmsI GCATC 3 cut(s) 217, 300, 1229
BpiI GAAGAC 2 cut(s) 447, 864
Bpu10I CCTNAGC 1 cut(s) 1203
Bpu14I TTCGAA 2 cut(s) 855, 1274
BpuEI CTTGAG 1 cut(s) 46
BsaAI YACGTR 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 314
Bsc4I CCNNNNNNNGG 1 cut(s) 677
Bse118I RCCGGY 1 cut(s) 998
Bse1I ACTGG 2 cut(s) 347, 934
Bse21I CCTNAGG 1 cut(s) 481
Bse3DI GCAATG 3 cut(s) 547, 671, 981
Bse8I GATNNNNATC 1 cut(s) 314
BseGI GGATG 2 cut(s) 780, 1056
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 677
BseMI GCAATG 3 cut(s) 547, 671, 981
BseMII CTCAG 2 cut(s) 495, 1217
BseNI ACTGG 2 cut(s) 347, 934
BseSI GKGCMC 1 cut(s) 701
BseXI GCAGC 9 cut(s) 67, 343, 488, 706, 728, 950, 973, 982, 989
BshFI GGCC 2 cut(s) 443, 730
BsiHKAI GWGCWC 1 cut(s) 1013
BsiSI CCGG 1 cut(s) 999
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 677
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 2 cut(s) 443, 730
Bsp119I TTCGAA 2 cut(s) 855, 1274
Bsp1286I GDGCHC 2 cut(s) 701, 1013
Bsp143I GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
BspACI CCGC 3 cut(s) 112, 132, 459
BspANI GGCC 2 cut(s) 443, 730
BspCNI CTCAG 2 cut(s) 494, 1216
BspHI TCATGA 1 cut(s) 498
BspMAI CTGCAG 3 cut(s) 478, 991, 1018
BspPI GGATC 3 cut(s) 182, 502, 559
BspT104I TTCGAA 2 cut(s) 855, 1274
BsrBI CCGCTC 1 cut(s) 461
BsrDI GCAATG 3 cut(s) 547, 671, 981
BsrFI RCCGGY 1 cut(s) 998
BsrI ACTGG 2 cut(s) 347, 934
BssAI RCCGGY 1 cut(s) 998
BssMI GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
Bst4CI ACNGT 4 cut(s) 418, 1076, 1255, 1281
BstAPI GCANNNNNTGC 2 cut(s) 983, 995
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 2 cut(s) 855, 1274
BstC8I GCNNGC 4 cut(s) 329, 412, 1081, 1199
BstDEI CTNAG 3 cut(s) 481, 1203, 1208
BstF5I GGATG 2 cut(s) 780, 1056
BstHHI GCGC 2 cut(s) 336, 540
BstKTI GATC 7 cut(s) 75, 177, 378, 510, 567, 805, 894
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
BstMWI GCNNNNNNNGC 6 cut(s) 333, 407, 983, 986, 992, 995
BstSFI CTRYAG 4 cut(s) 255, 474, 987, 1014
BstSLI GKGCMC 1 cut(s) 701
BstV1I GCAGC 9 cut(s) 67, 343, 488, 706, 728, 950, 973, 982, 989
BstV2I GAAGAC 2 cut(s) 447, 864
BstX2I RGATCY 4 cut(s) 174, 507, 564, 802
BstYI RGATCY 4 cut(s) 174, 507, 564, 802
Bsu36I CCTNAGG 1 cut(s) 481
BsuI GTATCC 1 cut(s) 1096
BsuRI GGCC 2 cut(s) 443, 730
BtsCI GGATG 2 cut(s) 780, 1056
BtsI GCAGTG 2 cut(s) 996, 1011
BtsIMutI CAGTG 5 cut(s) 414, 840, 884, 996, 1011
Cac8I GCNNGC 4 cut(s) 329, 412, 1081, 1199
CaiI CAGNNNCTG 2 cut(s) 416, 995
CciI TCATGA 1 cut(s) 498
CfoI GCGC 2 cut(s) 336, 540
Cfr10I RCCGGY 1 cut(s) 998
Cfr13I GGNCC 1 cut(s) 917
Csp6I GTAC 4 cut(s) 146, 187, 249, 661
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 6 cut(s) 373, 397, 499, 740, 1034, 1236
CviQI GTAC 4 cut(s) 146, 187, 249, 661
DdeI CTNAG 3 cut(s) 481, 1203, 1208
DpnI GATC 7 cut(s) 74, 176, 377, 509, 566, 804, 893
DpnII GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
DraIII CACNNNGTG 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 917
Eco57I CTGAAG 2 cut(s) 549, 908
Eco81I CCTNAGG 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 917
FaeI CATG 6 cut(s) 376, 400, 502, 743, 1037, 1239
FaiI YATR 9 cut(s) 276, 374, 398, 500, 686, 741, 1035, 1068, 1237
FalI AAGNNNNNCTT 2 cut(s) 1104, 1136
FaqI GGGAC 1 cut(s) 77
FatI CATG 6 cut(s) 372, 396, 498, 739, 1033, 1235
FbaI TGATCA 2 cut(s) 72, 375
Fnu4HI GCNGC 9 cut(s) 81, 332, 477, 717, 720, 939, 978, 987, 996
FokI GGATG 2 cut(s) 787, 1063
Fsp4HI GCNGC 9 cut(s) 81, 332, 477, 717, 720, 939, 978, 987, 996
FspBI CTAG 6 cut(s) 54, 246, 284, 524, 590, 771
GlaI GCGC 2 cut(s) 335, 539
GluI GCNGC 9 cut(s) 81, 332, 477, 717, 720, 939, 978, 987, 996
HaeIII GGCC 2 cut(s) 443, 730
HapII CCGG 1 cut(s) 999
HhaI GCGC 2 cut(s) 336, 540
Hin1II CATG 6 cut(s) 376, 400, 502, 743, 1037, 1239
Hin6I GCGC 2 cut(s) 334, 538
HinP1I GCGC 2 cut(s) 334, 538
HinfI GANTC 2 cut(s) 108, 215
HpaII CCGG 1 cut(s) 999
HphI GGTGA 3 cut(s) 109, 850, 1166
Hpy188I TCNGA 1 cut(s) 1286
Hpy188III TCNNGA 6 cut(s) 212, 483, 499, 599, 650, 806
HpyAV CCTTC 3 cut(s) 146, 525, 616
HpyCH4III ACNGT 4 cut(s) 418, 1076, 1255, 1281
HpyCH4IV ACGT 2 cut(s) 185, 348
HpyF10VI GCNNNNNNNGC 6 cut(s) 333, 407, 983, 986, 992, 995
HpyF3I CTNAG 3 cut(s) 481, 1203, 1208
HpySE526I ACGT 2 cut(s) 185, 348
Hsp92II CATG 6 cut(s) 376, 400, 502, 743, 1037, 1239
HspAI GCGC 2 cut(s) 334, 538
Ksp22I TGATCA 2 cut(s) 72, 375
Kzo9I GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
LmnI GCTCC 1 cut(s) 680
LpnPI CCDG 9 cut(s) 328, 396, 411, 468, 900, 915, 1012, 1065, 1211
Lsp1109I GCAGC 9 cut(s) 67, 343, 488, 706, 728, 950, 973, 982, 989
LweI GCATC 3 cut(s) 217, 300, 1229
MaeI CTAG 6 cut(s) 54, 246, 284, 524, 590, 771
MaeII ACGT 2 cut(s) 185, 348
MaeIII GTNAC 2 cut(s) 875, 962
MalI GATC 7 cut(s) 74, 176, 377, 509, 566, 804, 893
MbiI CCGCTC 1 cut(s) 461
MboI GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
MfeI CAATTG 2 cut(s) 161, 1107
MflI RGATCY 4 cut(s) 174, 507, 564, 802
MhlI GDGCHC 2 cut(s) 701, 1013
MluCI AATT 8 cut(s) 161, 303, 405, 825, 1094, 1107, 1157, 1184
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 7 cut(s) 70, 280, 400, 490, 706, 1093, 1212
MroXI GAANNNNTTC 1 cut(s) 1280
MslI CAYNNNNRTG 2 cut(s) 371, 969
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 1 cut(s) 999
MunI CAATTG 2 cut(s) 161, 1107
Mva1269I GAATGC 1 cut(s) 435
MwoI GCNNNNNNNGC 6 cut(s) 333, 407, 983, 986, 992, 995
NdeII GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
NlaIII CATG 6 cut(s) 376, 400, 502, 743, 1037, 1239
NmuCI GTSAC 1 cut(s) 962
NspV TTCGAA 2 cut(s) 855, 1274
PagI TCATGA 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 861
PctI GAATGC 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 1280
PfeI GAWTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 9 cut(s) 82, 333, 478, 718, 721, 940, 979, 988, 997
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
Ppu21I YACGTR 1 cut(s) 186
PpuMI RGGWCCY 1 cut(s) 917
Psp1406I AACGTT 1 cut(s) 348
Psp5II RGGWCCY 1 cut(s) 917
PspPI GGNCC 1 cut(s) 917
PspPPI RGGWCCY 1 cut(s) 917
PstI CTGCAG 3 cut(s) 478, 991, 1018
PstNI CAGNNNCTG 2 cut(s) 416, 995
PsuI RGATCY 4 cut(s) 174, 507, 564, 802
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 4 cut(s) 147, 188, 250, 662
RsaNI GTAC 4 cut(s) 146, 187, 249, 661
RseI CAYNNNNRTG 2 cut(s) 371, 969
SatI GCNGC 9 cut(s) 81, 332, 477, 717, 720, 939, 978, 987, 996
Sau3AI GATC 7 cut(s) 72, 174, 375, 507, 564, 802, 891
Sau96I GGNCC 1 cut(s) 917
ScaI AGTACT 1 cut(s) 250
SchI GAGTC 1 cut(s) 102
SduI GDGCHC 2 cut(s) 701, 1013
SfaNI GCATC 3 cut(s) 217, 300, 1229
SfcI CTRYAG 4 cut(s) 255, 474, 987, 1014
SfuI TTCGAA 2 cut(s) 855, 1274
SinI GGWCC 1 cut(s) 917
SmiMI CAYNNNNRTG 2 cut(s) 371, 969
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SpeI ACTAGT 1 cut(s) 245
Sse9I AATT 8 cut(s) 161, 303, 405, 825, 1094, 1107, 1157, 1184
SsiI CCGC 3 cut(s) 112, 132, 459
SspMI CTAG 6 cut(s) 54, 246, 284, 524, 590, 771
TaaI ACNGT 4 cut(s) 418, 1076, 1255, 1281
TaiI ACGT 2 cut(s) 188, 351
TaqI TCGA 2 cut(s) 855, 1274
TasI AATT 8 cut(s) 161, 303, 405, 825, 1094, 1107, 1157, 1184
TatI WGTACW 1 cut(s) 248
TfiI GAWTC 1 cut(s) 215
TscAI CASTG 5 cut(s) 421, 840, 891, 996, 1018
TseFI GTSAC 1 cut(s) 962
TseI GCWGC 9 cut(s) 80, 331, 476, 716, 719, 938, 977, 986, 995
Tsp45I GTSAC 1 cut(s) 962
TspDTI ATGAA 6 cut(s) 221, 515, 828, 993, 1184, 1224
TspRI CASTG 5 cut(s) 421, 840, 891, 996, 1018
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 917
XcmI CCANNNNNNNNNTGG 1 cut(s) 988
XmnI GAANNNNTTC 1 cut(s) 1280
XspI CTAG 6 cut(s) 54, 246, 284, 524, 590, 771
ZrmI AGTACT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.