FvH4_3g01810

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009170
Physical Location & Seq
Forward (+)
931371 .. 932945
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01810.t1

Sequence Viewer

Length: 1284 bp
ATGGCTTCTTTGCAAACTTCAAGTCTCTTATTCCCTGCATCTTCAACTCTTTCTTGTTCCTCAAGGAGAATGATCAATGCTGCAATCCATGTCCCCAAAGTTCCAAGACTCCGCTTCACCAACCCGAAAACCGCAACAAAGTTGGTACTTGAAGGCCTACAACTGAGTGATGGGATCTCAAACACATACCAAGTAGCTCAACCAAAGCATCAAGAATCATCAAACCACATTCATTCTACTACCACTACTTCTACAGCTACCATTCAGCTCTATGCAATCCTAGAGGAAGTAGCAGACAGAATTGAGATGCACATCAATGTCGGCGAGCAACGTAACAACTGGAACATTCTTCTGCTCAACTCCATCAACATGATCACTCTCACAGCCTCAGTCATGGCTGGAATTGCAGGCACTGTTGGTGATGGAACTCCCATTTTGGCCTTGAAGTTGTCTTCCGCTCTTTTATTCACTGCAGCCTCAGGAATGTTGCTTATCATGAACAAGATCCAACCTTCACAACTAGCTGAAGAACAGCGCAATGCTACAAGATTGTTCAAGCAAATCCAAACTCAAATCCATACCTTACTAGCACTTCGTGAGCCGACAGAACAAGATGTGAAGGAAACAATGGAGAAGGTTTTGGCCCTTGACAAAGCCTACCCTCTTCCATTGCTTGGAGCTATGCTTGAAAAGTACCCTAAAAAGTTTGAGGCAGCTGCTTGGTGGCCTAAAAGTCATGGAGTTCAAAAACCAAGCGAAACGAAATCAAGGATGGGAAAGACTAATGGGTGGAGTGAAGATCTTGAAGATGAAATGAGAGAAATTATTTCAGTGGTGAAGAGCAAAGACTTCGAAGACTACGAGAGATTAGGTAACATTGCACTCAAGATCAACAAGGTTTTGGCTATCTCTGGTCCTTTGCTTACTGGAGTTGCAGCCGTTGGGTCTGCTTTTGTGGGTCACAATGATATTGCAGCCATTGCAGCAGTGGCTGCAGGTTCATTGAGCACTGCAGTCAATGCTTTTCAACATGGTGCTCAAGTTGGGATGGTGTTTGAGATGTATAGGTACTGTGCTGGCTTCTTCAACAAATTGGAGGATACAATTGAAGCCACACTTGGGGAACAAGATTTGGAGAAAAGAGAAAATGGTGAATTGTTTGAGATGAAGATTGCTATGCAATTGGGAAGAAACTTGCCTCAGCTTAGAGAACTTGCATCAAAATCAGCTTCATGTAGGAGAAATGGAACAGTTGTAGATGAAATGGGTTCAAGACTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

428

Amino Acids

46.82

Weight (kDa)

7.14

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 89 - 403 2.3e-154 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 986
AccB7I CCANNNNNTGG 1 cut(s) 674
AccBSI CCGCTC 1 cut(s) 458
AciI CCGC 3 cut(s) 112, 132, 456
AclWI GGATC 2 cut(s) 182, 499
AcuI CTGAAG 1 cut(s) 546
AdeI CACNNNGTG 1 cut(s) 596
AfaI GTAC 3 cut(s) 147, 695, 1070
AfiI CCNNNNNNNGG 2 cut(s) 674, 1119
AjuI GAANNNNNNNTTGG 2 cut(s) 1101, 1133
AluBI AGCT 8 cut(s) 197, 257, 268, 524, 680, 716, 1204, 1229
AluI AGCT 8 cut(s) 197, 257, 268, 524, 680, 716, 1204, 1229
Alw21I GWGCWC 2 cut(s) 1010, 1039
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 2 cut(s) 182, 499
AlwNI CAGNNNCTG 2 cut(s) 413, 992
AoxI GGCC 4 cut(s) 154, 438, 642, 725
ApeKI GCWGC 8 cut(s) 80, 473, 713, 716, 935, 974, 983, 992
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 2 cut(s) 643, 914
AsuHPI GGTGA 4 cut(s) 109, 431, 847, 1163
AsuII TTCGAA 1 cut(s) 852
AvaII GGWCC 1 cut(s) 914
AxyI CCTNAGG 1 cut(s) 478
BbsI GAAGAC 2 cut(s) 444, 861
Bbv12I GWGCWC 2 cut(s) 1010, 1039
BbvCI CCTCAGC 1 cut(s) 1200
BbvI GCAGC 8 cut(s) 67, 485, 703, 725, 947, 979, 986, 995
BccI CCATC 5 cut(s) 164, 371, 416, 766, 1042
BceAI ACGGC 1 cut(s) 923
BcgI CGANNNNNNTGC 2 cut(s) 832, 866
BciVI GTATCC 1 cut(s) 1093
BclI TGATCA 2 cut(s) 72, 372
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 3 cut(s) 281, 521, 587
BfmI CTRYAG 4 cut(s) 252, 471, 993, 1011
BfuAI ACCTGC 1 cut(s) 986
BfuI GTATCC 1 cut(s) 1093
BglII AGATCT 1 cut(s) 799
BisI GCNGC 8 cut(s) 81, 474, 714, 717, 936, 975, 984, 993
BlsI GCNGC 8 cut(s) 82, 475, 715, 718, 937, 976, 985, 994
Bme18I GGWCC 1 cut(s) 914
BmgT120I GGNCC 2 cut(s) 643, 914
BmsI GCATC 4 cut(s) 47, 217, 297, 1226
BpiI GAAGAC 2 cut(s) 444, 861
BpmI CTGGAG 1 cut(s) 948
Bpu10I CCTNAGC 1 cut(s) 1200
Bpu14I TTCGAA 1 cut(s) 852
BpuEI CTTGAG 3 cut(s) 46, 869, 1023
BsaBI GATNNNNATC 1 cut(s) 311
Bsc4I CCNNNNNNNGG 2 cut(s) 674, 1119
Bse1I ACTGG 2 cut(s) 344, 931
Bse21I CCTNAGG 1 cut(s) 478
Bse3DI GCAATG 4 cut(s) 544, 668, 876, 978
Bse8I GATNNNNATC 1 cut(s) 311
BseGI GGATG 2 cut(s) 777, 1053
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 674, 1119
BseMI GCAATG 4 cut(s) 544, 668, 876, 978
BseMII CTCAG 4 cut(s) 155, 402, 492, 1214
BseNI ACTGG 2 cut(s) 344, 931
BseXI GCAGC 8 cut(s) 67, 485, 703, 725, 947, 979, 986, 995
BshFI GGCC 4 cut(s) 156, 440, 644, 727
BsiHKAI GWGCWC 2 cut(s) 1010, 1039
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 674, 1119
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 4 cut(s) 156, 440, 644, 727
Bsp119I TTCGAA 1 cut(s) 852
Bsp1286I GDGCHC 2 cut(s) 1010, 1039
Bsp143I GATC 6 cut(s) 72, 174, 372, 504, 799, 888
BspACI CCGC 3 cut(s) 112, 132, 456
BspANI GGCC 4 cut(s) 156, 440, 644, 727
BspCNI CTCAG 4 cut(s) 156, 401, 491, 1213
BspHI TCATGA 1 cut(s) 495
BspMAI CTGCAG 3 cut(s) 475, 997, 1015
BspMI ACCTGC 1 cut(s) 986
BspPI GGATC 2 cut(s) 182, 499
BspQI GCTCTTC 1 cut(s) 833
BspT104I TTCGAA 1 cut(s) 852
BsrBI CCGCTC 1 cut(s) 458
BsrDI GCAATG 4 cut(s) 544, 668, 876, 978
BsrI ACTGG 2 cut(s) 344, 931
BssMI GATC 6 cut(s) 72, 174, 372, 504, 799, 888
Bst4CI ACNGT 4 cut(s) 415, 1073, 1252, 1278
Bst6I CTCTTC 2 cut(s) 669, 833
BstAPI GCANNNNNTGC 3 cut(s) 980, 992, 1019
BstBI TTCGAA 1 cut(s) 852
BstC8I GCNNGC 3 cut(s) 326, 409, 1078
BstDEI CTNAG 5 cut(s) 164, 388, 478, 1200, 1205
BstF5I GGATG 2 cut(s) 777, 1053
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 6 cut(s) 75, 177, 375, 507, 802, 891
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 6 cut(s) 72, 174, 372, 504, 799, 888
BstMWI GCNNNNNNNGC 6 cut(s) 404, 980, 983, 989, 992, 1019
BstSFI CTRYAG 4 cut(s) 252, 471, 993, 1011
BstV1I GCAGC 8 cut(s) 67, 485, 703, 725, 947, 979, 986, 995
BstV2I GAAGAC 2 cut(s) 444, 861
BstX2I RGATCY 3 cut(s) 174, 504, 799
BstYI RGATCY 3 cut(s) 174, 504, 799
Bsu36I CCTNAGG 1 cut(s) 478
BsuI GTATCC 1 cut(s) 1093
BsuRI GGCC 4 cut(s) 156, 440, 644, 727
BtsCI GGATG 2 cut(s) 777, 1053
BtsI GCAGTG 3 cut(s) 468, 993, 1008
BtsIMutI CAGTG 5 cut(s) 411, 468, 837, 993, 1008
BveI ACCTGC 1 cut(s) 986
Cac8I GCNNGC 3 cut(s) 326, 409, 1078
CaiI CAGNNNCTG 2 cut(s) 413, 992
CciI TCATGA 1 cut(s) 495
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 2 cut(s) 643, 914
Csp6I GTAC 3 cut(s) 146, 694, 1069
CviAII CATG 7 cut(s) 89, 370, 394, 496, 737, 1031, 1233
CviQI GTAC 3 cut(s) 146, 694, 1069
DdeI CTNAG 5 cut(s) 164, 388, 478, 1200, 1205
DpnI GATC 6 cut(s) 74, 176, 374, 506, 801, 890
DpnII GATC 6 cut(s) 72, 174, 372, 504, 799, 888
DraIII CACNNNGTG 1 cut(s) 596
Eam1104I CTCTTC 2 cut(s) 669, 833
EarI CTCTTC 2 cut(s) 669, 833
Eco147I AGGCCT 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 914
Eco57I CTGAAG 1 cut(s) 546
Eco81I CCTNAGG 1 cut(s) 478
FaeI CATG 7 cut(s) 92, 373, 397, 499, 740, 1034, 1236
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 1 cut(s) 77
FatI CATG 7 cut(s) 88, 369, 393, 495, 736, 1030, 1232
FbaI TGATCA 2 cut(s) 72, 372
Fnu4HI GCNGC 8 cut(s) 81, 474, 714, 717, 936, 975, 984, 993
FokI GGATG 2 cut(s) 784, 1060
Fsp4HI GCNGC 8 cut(s) 81, 474, 714, 717, 936, 975, 984, 993
FspBI CTAG 3 cut(s) 281, 521, 587
GlaI GCGC 1 cut(s) 536
GluI GCNGC 8 cut(s) 81, 474, 714, 717, 936, 975, 984, 993
GsuI CTGGAG 1 cut(s) 948
HaeIII GGCC 4 cut(s) 156, 440, 644, 727
HhaI GCGC 1 cut(s) 537
Hin1II CATG 7 cut(s) 92, 373, 397, 499, 740, 1034, 1236
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 2 cut(s) 108, 215
HphI GGTGA 4 cut(s) 109, 431, 847, 1163
Hpy188I TCNGA 1 cut(s) 1283
Hpy188III TCNNGA 7 cut(s) 212, 480, 496, 596, 803, 886, 1272
HpyAV CCTTC 4 cut(s) 146, 522, 613, 628
HpyCH4III ACNGT 4 cut(s) 415, 1073, 1252, 1278
HpyCH4IV ACGT 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 6 cut(s) 404, 980, 983, 989, 992, 1019
HpyF3I CTNAG 5 cut(s) 164, 388, 478, 1200, 1205
HpySE526I ACGT 1 cut(s) 331
Hsp92II CATG 7 cut(s) 92, 373, 397, 499, 740, 1034, 1236
HspAI GCGC 1 cut(s) 535
Ksp22I TGATCA 2 cut(s) 72, 372
Kzo9I GATC 6 cut(s) 72, 174, 372, 504, 799, 888
LguI GCTCTTC 1 cut(s) 833
LmnI GCTCC 1 cut(s) 677
LpnPI CCDG 9 cut(s) 48, 325, 384, 393, 465, 897, 912, 981, 1062
Lsp1109I GCAGC 8 cut(s) 67, 485, 703, 725, 947, 979, 986, 995
LweI GCATC 4 cut(s) 47, 217, 297, 1226
MaeI CTAG 3 cut(s) 281, 521, 587
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 3 cut(s) 332, 872, 959
MalI GATC 6 cut(s) 74, 176, 374, 506, 801, 890
MbiI CCGCTC 1 cut(s) 458
MboI GATC 6 cut(s) 72, 174, 372, 504, 799, 888
MfeI CAATTG 2 cut(s) 1104, 1181
MflI RGATCY 3 cut(s) 174, 504, 799
MhlI GDGCHC 2 cut(s) 1010, 1039
MluCI AATT 7 cut(s) 300, 402, 822, 1091, 1104, 1154, 1181
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 8 cut(s) 70, 277, 397, 487, 672, 703, 1090, 1209
MslI CAYNNNNRTG 2 cut(s) 315, 368
MspA1I CMGCKG 1 cut(s) 716
MunI CAATTG 2 cut(s) 1104, 1181
MwoI GCNNNNNNNGC 6 cut(s) 404, 980, 983, 989, 992, 1019
NdeII GATC 6 cut(s) 72, 174, 372, 504, 799, 888
NlaIII CATG 7 cut(s) 92, 373, 397, 499, 740, 1034, 1236
NmuCI GTSAC 1 cut(s) 959
NspV TTCGAA 1 cut(s) 852
PagI TCATGA 1 cut(s) 495
PceI AGGCCT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 833
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PfeI GAWTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 8 cut(s) 82, 475, 715, 718, 937, 976, 985, 994
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 643, 914
PstI CTGCAG 3 cut(s) 475, 997, 1015
PstNI CAGNNNCTG 2 cut(s) 413, 992
PsuI RGATCY 3 cut(s) 174, 504, 799
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 3 cut(s) 147, 695, 1070
RsaNI GTAC 3 cut(s) 146, 694, 1069
RseI CAYNNNNRTG 2 cut(s) 315, 368
SapI GCTCTTC 1 cut(s) 833
SatI GCNGC 8 cut(s) 81, 474, 714, 717, 936, 975, 984, 993
Sau3AI GATC 6 cut(s) 72, 174, 372, 504, 799, 888
Sau96I GGNCC 2 cut(s) 643, 914
SchI GAGTC 1 cut(s) 102
SduI GDGCHC 2 cut(s) 1010, 1039
SfaNI GCATC 4 cut(s) 47, 217, 297, 1226
SfcI CTRYAG 4 cut(s) 252, 471, 993, 1011
SfuI TTCGAA 1 cut(s) 852
SinI GGWCC 1 cut(s) 914
SmiMI CAYNNNNRTG 2 cut(s) 315, 368
SmlI CTYRAG 3 cut(s) 61, 884, 1038
SmoI CTYRAG 3 cut(s) 61, 884, 1038
Sse9I AATT 7 cut(s) 300, 402, 822, 1091, 1104, 1154, 1181
SseBI AGGCCT 1 cut(s) 156
SsiI CCGC 3 cut(s) 112, 132, 456
SspMI CTAG 3 cut(s) 281, 521, 587
StuI AGGCCT 1 cut(s) 156
TaaI ACNGT 4 cut(s) 415, 1073, 1252, 1278
TaiI ACGT 1 cut(s) 334
TaqI TCGA 1 cut(s) 852
TasI AATT 7 cut(s) 300, 402, 822, 1091, 1104, 1154, 1181
TfiI GAWTC 1 cut(s) 215
TscAI CASTG 5 cut(s) 418, 475, 837, 993, 1015
TseFI GTSAC 1 cut(s) 959
TseI GCWGC 8 cut(s) 80, 473, 713, 716, 935, 974, 983, 992
Tsp45I GTSAC 1 cut(s) 959
TspDTI ATGAA 7 cut(s) 221, 512, 825, 990, 1181, 1221, 1275
TspRI CASTG 5 cut(s) 418, 475, 837, 993, 1015
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 914
XcmI CCANNNNNNNNNTGG 1 cut(s) 985
XspI CTAG 3 cut(s) 281, 521, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.