MD10G1323800.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009170
Physical Location & Seq
Reverse (-)
40468681 .. 40469952
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1323800.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGCTTCCCTCCAAACTTCATCTCTCCTCTTCTCTTCTCCCTCATGTTGTTCTTCCTCAAACAGAACCATAAATGCCGCCATTCATGTCCCCAAGCTCCCAAGAGTCCGCTTCACTGTCCCCAAACAAACGACGAAGCTCGTCCTCGAAGGCCTCCAGTTGAAGGAACAAGTTCTGAAACACGAAAACATCGATTTGTATAATAACAATCAGTCTGCAGCAACAGCAACTCCTACAGTTTCCACAGCTCAGCTCTACGCGCTCTTAGAGGCGGTGGCGGACAGAGTGGAGATGCACGCAAACATTGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCACTCTCTCCGCTTCCCTCATGGCCGGAGTTAATGCAGCTGCTGGAACGTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCCACCGGAATGCTGCTTATCATGAACAAGATCCAGCCTTCGCAGCTCGCCGAGGAGCAGCGCAACGCTACGAGGTTGTTCAGGCAGCTCCAGAGTGAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGGTTTTGGCTCTTGACAAAGCTTACCCTCTTCCGTTACTTGGTGCCATGCTCGAAAAATTCCCCAAAAAATTTGAGCCGGCTGTTTGGTGGCCTAGAAATGTTGTTCAATCCCAAAATCCAAGGAAACTGAAATCCCAGAAGGGAAAGAATGTGAATGGATGGAGTGAAGAACTTGAAAGTGAAATGAGAGAAATTATTCAAGTTGTGAAGAGCAAGGACATTGAAGACTATGAGAGATTAGGGAGCTTGGTTCTAAAGATCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGGGATTGCGGCTGTTGGATCCGCTTTTGTAGGCCATGGATCAGCCACTGCCGCGGTTGCAGCAGTGGCTGCAGGCTCGTTGGCTAGCATAGTGAATGCTTTTCAGCATGGCGCGCAAGTAGGGATGGTATTTGAGATGTATAGAAACTGCGCCGGCTTCTTTAACAAGTTGGAAGATTCAATTGAAGCAACACTTGGAGAACAAGACTTGGAGAAGAGAGAAAATGGGGGGATTTTCGAAATGAAGGTTGCAATGCAGTTGGGAAGAAGCTTGGAGCAACTCAGAGAACTTGCATCGAAATCAGCTTTGTATCGTTCGAACGGAGAACCAATAGACGAATTCGGTTGCAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

424

Amino Acids

46.17

Weight (kDa)

7.65

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 84 - 399 4.2e-156 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 656
AccII CGCG 3 cut(s) 260, 965, 1025
AclWI GGATC 5 cut(s) 478, 572, 924, 937, 958
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 3 cut(s) 671, 683, 1250
AfiI CCNNNNNNNGG 2 cut(s) 163, 653
AgsI TTSAA 8 cut(s) 163, 424, 722, 791, 815, 839, 1092, 1097
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
Alw26I GTCTC 2 cut(s) 551, 596
AlwI GGATC 5 cut(s) 478, 572, 924, 937, 958
AlwNI CAGNNNCTG 3 cut(s) 401, 959, 980
AoxI GGCC 5 cut(s) 151, 381, 704, 898, 943
ApaI GGGCCC 1 cut(s) 902
ApoI RAATTY 3 cut(s) 671, 683, 1250
Asp700I GAANNNNTTC 2 cut(s) 171, 810
AspLEI GCGC 6 cut(s) 262, 318, 516, 1025, 1027, 1064
AspS9I GGNCC 2 cut(s) 898, 899
AsuII TTCGAA 2 cut(s) 1149, 1229
AsuNHI GCTAGC 1 cut(s) 995
BaeGI GKGCMC 1 cut(s) 902
BamHI GGATCC 1 cut(s) 929
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 902
BbsI GAAGAC 1 cut(s) 846
BccI CCATC 2 cut(s) 768, 1030
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 551, 596
BfaI CTAG 2 cut(s) 708, 996
BfmI CTRYAG 3 cut(s) 216, 234, 981
BlpI GCTNAGC 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 898, 899
BmiI GGNNCC 3 cut(s) 658, 900, 931
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 2 cut(s) 282, 1214
BmtI GCTAGC 1 cut(s) 999
BmuI ACTGGG 1 cut(s) 921
BpiI GAAGAC 1 cut(s) 846
BpmI CTGGAG 2 cut(s) 140, 527
Bpu1102I GCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 2 cut(s) 1149, 1229
Bsa29I ATCGAT 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 504, 734, 946, 963
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 653
Bse118I RCCGGY 2 cut(s) 691, 1064
Bse1I ACTGG 3 cut(s) 157, 329, 916
Bse3DI GCAATG 1 cut(s) 1170
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 4 cut(s) 504, 734, 946, 963
BseGI GGATG 2 cut(s) 779, 1041
BseLI CCNNNNNNNGG 2 cut(s) 163, 653
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 3 cut(s) 263, 576, 1207
BseNI ACTGG 3 cut(s) 157, 329, 916
BsePI GCGCGC 1 cut(s) 1023
BseRI GAGGAG 2 cut(s) 17, 521
BseSI GKGCMC 1 cut(s) 902
Bsh1236I CGCG 3 cut(s) 260, 965, 1025
BshFI GGCC 5 cut(s) 153, 383, 706, 900, 945
BshNI GGYRCC 1 cut(s) 656
BshVI ATCGAT 1 cut(s) 192
BsiSI CCGG 4 cut(s) 384, 459, 692, 1065
BslFI GGGAC 2 cut(s) 74, 104
BslI CCNNNNNNNGG 2 cut(s) 163, 653
BsmAI GTCTC 2 cut(s) 551, 596
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 74, 104
BsmI GAATGC 2 cut(s) 468, 1012
BsnI GGCC 5 cut(s) 153, 383, 706, 900, 945
Bsp119I TTCGAA 2 cut(s) 1149, 1229
Bsp120I GGGCCC 1 cut(s) 898
Bsp1286I GDGCHC 1 cut(s) 902
Bsp143I GATC 6 cut(s) 357, 483, 577, 873, 929, 950
Bsp1720I GCTNAGC 1 cut(s) 249
Bsp19I CCATGG 1 cut(s) 946
BspANI GGCC 5 cut(s) 153, 383, 706, 900, 945
BspCNI CTCAG 3 cut(s) 262, 577, 1206
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 3 cut(s) 260, 965, 1025
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 658, 900, 931
BspMAI CTGCAG 2 cut(s) 220, 985
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 5 cut(s) 478, 572, 924, 937, 958
BspQI GCTCTTC 2 cut(s) 576, 818
BspT104I TTCGAA 2 cut(s) 1149, 1229
BspT107I GGYRCC 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 1170
BsrFI RCCGGY 2 cut(s) 691, 1064
BsrI ACTGG 3 cut(s) 157, 329, 916
BssAI RCCGGY 2 cut(s) 691, 1064
BssECI CCNNGG 4 cut(s) 504, 734, 946, 963
BssHII GCGCGC 1 cut(s) 1023
BssMI GATC 6 cut(s) 357, 483, 577, 873, 929, 950
BssT1I CCWWGG 2 cut(s) 734, 946
Bst4CI ACNGT 3 cut(s) 118, 238, 562
Bst6I CTCTTC 6 cut(s) 35, 40, 576, 648, 818, 1121
BstAPI GCANNNNNTGC 1 cut(s) 980
BstBI TTCGAA 2 cut(s) 1149, 1229
BstC8I GCNNGC 9 cut(s) 297, 311, 501, 693, 985, 997, 1025, 1066, 1262
BstDEI CTNAG 5 cut(s) 249, 265, 341, 585, 1193
BstDSI CCRYGG 2 cut(s) 946, 963
BstF5I GGATG 2 cut(s) 779, 1041
BstFNI CGCG 3 cut(s) 260, 965, 1025
BstHHI GCGC 6 cut(s) 262, 318, 516, 1025, 1027, 1064
BstKTI GATC 6 cut(s) 360, 486, 580, 876, 932, 953
BstMAI GTCTC 2 cut(s) 551, 596
BstMBI GATC 6 cut(s) 357, 483, 577, 873, 929, 950
BstSFI CTRYAG 3 cut(s) 216, 234, 981
BstSLI GKGCMC 1 cut(s) 902
BstUI CGCG 3 cut(s) 260, 965, 1025
BstV2I GAAGAC 1 cut(s) 846
BstX2I RGATCY 2 cut(s) 483, 929
BstYI RGATCY 2 cut(s) 483, 929
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 5 cut(s) 153, 383, 706, 900, 945
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 2 cut(s) 946, 963
BtsCI GGATG 2 cut(s) 779, 1041
BtsI GCAGTG 2 cut(s) 957, 981
BtsIMutI CAGTG 3 cut(s) 114, 957, 981
Cac8I GCNNGC 9 cut(s) 297, 311, 501, 693, 985, 997, 1025, 1066, 1262
CaiI CAGNNNCTG 3 cut(s) 401, 959, 980
CciI TCATGA 1 cut(s) 474
CfoI GCGC 6 cut(s) 262, 318, 516, 1025, 1027, 1064
Cfr10I RCCGGY 2 cut(s) 691, 1064
Cfr13I GGNCC 2 cut(s) 898, 899
Cfr42I CCGCGG 1 cut(s) 966
ClaI ATCGAT 1 cut(s) 192
CviAII CATG 8 cut(s) 45, 86, 355, 379, 475, 661, 947, 1019
DdeI CTNAG 5 cut(s) 249, 265, 341, 585, 1193
DpnI GATC 6 cut(s) 359, 485, 579, 875, 931, 952
DpnII GATC 6 cut(s) 357, 483, 577, 873, 929, 950
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 6 cut(s) 35, 40, 576, 648, 818, 1121
EarI CTCTTC 6 cut(s) 35, 40, 576, 648, 818, 1121
EciI GGCGGA 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 734, 946
Eco147I AGGCCT 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 902
EcoO109I RGGNCCY 1 cut(s) 899
EcoRI GAATTC 1 cut(s) 1250
EcoT14I CCWWGG 2 cut(s) 734, 946
EcoT38I GRGCYC 1 cut(s) 902
ErhI CCWWGG 2 cut(s) 734, 946
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 8 cut(s) 48, 89, 358, 382, 478, 664, 950, 1022
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 2 cut(s) 74, 104
FatI CATG 8 cut(s) 44, 85, 354, 378, 474, 660, 946, 1018
FbaI TGATCA 1 cut(s) 357
FokI GGATG 2 cut(s) 786, 1048
FriOI GRGCYC 1 cut(s) 902
FspBI CTAG 2 cut(s) 708, 996
GlaI GCGC 6 cut(s) 261, 317, 515, 1024, 1026, 1063
GsuI CTGGAG 2 cut(s) 140, 527
HaeIII GGCC 5 cut(s) 153, 383, 706, 900, 945
HapII CCGG 4 cut(s) 384, 459, 692, 1065
HhaI GCGC 6 cut(s) 262, 318, 516, 1025, 1027, 1064
Hin1II CATG 8 cut(s) 48, 89, 358, 382, 478, 664, 950, 1022
Hin6I GCGC 6 cut(s) 260, 316, 514, 1023, 1025, 1062
HinP1I GCGC 6 cut(s) 260, 316, 514, 1023, 1025, 1062
HindIII AAGCTT 3 cut(s) 633, 1180, 1262
HinfI GANTC 2 cut(s) 105, 1088
HpaII CCGG 4 cut(s) 384, 459, 692, 1065
Hpy188I TCNGA 2 cut(s) 177, 1196
Hpy188III TCNNGA 4 cut(s) 475, 544, 575, 626
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 7 cut(s) 143, 157, 501, 592, 607, 748, 1150
HpyCH4III ACNGT 3 cut(s) 118, 238, 562
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 9 cut(s) 218, 295, 395, 971, 983, 1163, 1168, 1205, 1260
HpyF3I CTNAG 5 cut(s) 249, 265, 341, 585, 1193
HpySE526I ACGT 1 cut(s) 407
Hsp92II CATG 8 cut(s) 48, 89, 358, 382, 478, 664, 950, 1022
HspAI GCGC 6 cut(s) 260, 316, 514, 1023, 1025, 1062
KroI GCCGGC 2 cut(s) 691, 1064
KroNI GCCGGC 2 cut(s) 693, 1066
Ksp22I TGATCA 1 cut(s) 357
KspI CCGCGG 1 cut(s) 966
Kzo9I GATC 6 cut(s) 357, 483, 577, 873, 929, 950
LguI GCTCTTC 2 cut(s) 576, 818
LmnI GCTCC 5 cut(s) 102, 508, 546, 858, 1186
LweI GCATC 2 cut(s) 282, 1214
MaeI CTAG 2 cut(s) 708, 996
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 648
MalI GATC 6 cut(s) 359, 485, 579, 875, 931, 952
MboI GATC 6 cut(s) 357, 483, 577, 873, 929, 950
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 2 cut(s) 483, 929
MhlI GDGCHC 1 cut(s) 902
MluCI AATT 6 cut(s) 552, 671, 683, 807, 1092, 1250
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 907, 1062
MroNI GCCGGC 2 cut(s) 691, 1064
MroXI GAANNNNTTC 2 cut(s) 171, 810
MseI TTAA 2 cut(s) 390, 1074
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 2 cut(s) 398, 965
MspI CCGG 4 cut(s) 384, 459, 692, 1065
MunI CAATTG 1 cut(s) 1092
Mva1269I GAATGC 2 cut(s) 468, 1012
MvnI CGCG 3 cut(s) 260, 965, 1025
NaeI GCCGGC 2 cut(s) 693, 1066
NcoI CCATGG 1 cut(s) 946
NdeII GATC 6 cut(s) 357, 483, 577, 873, 929, 950
NgoMIV GCCGGC 2 cut(s) 691, 1064
NheI GCTAGC 1 cut(s) 995
NlaIII CATG 8 cut(s) 48, 89, 358, 382, 478, 664, 950, 1022
NlaIV GGNNCC 3 cut(s) 658, 900, 931
NmeAIII GCCGAG 1 cut(s) 529
NspV TTCGAA 2 cut(s) 1149, 1229
PagI TCATGA 1 cut(s) 474
PauI GCGCGC 1 cut(s) 1023
PceI AGGCCT 1 cut(s) 153
PciSI GCTCTTC 2 cut(s) 576, 818
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 2 cut(s) 468, 1012
PdiI GCCGGC 2 cut(s) 693, 1066
PdmI GAANNNNTTC 2 cut(s) 171, 810
PfeI GAWTC 1 cut(s) 1088
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PspN4I GGNNCC 3 cut(s) 658, 900, 931
PspOMI GGGCCC 1 cut(s) 898
PspPI GGNCC 2 cut(s) 898, 899
PstI CTGCAG 2 cut(s) 220, 985
PstNI CAGNNNCTG 3 cut(s) 401, 959, 980
PsuI RGATCY 2 cut(s) 483, 929
PteI GCGCGC 1 cut(s) 1023
PvuII CAGCTG 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 461
SacII CCGCGG 1 cut(s) 966
SapI GCTCTTC 2 cut(s) 576, 818
SaqAI TTAA 2 cut(s) 390, 1074
Sau3AI GATC 6 cut(s) 357, 483, 577, 873, 929, 950
Sau96I GGNCC 2 cut(s) 898, 899
SchI GAGTC 1 cut(s) 114
SduI GDGCHC 1 cut(s) 902
SfaNI GCATC 2 cut(s) 282, 1214
SfcI CTRYAG 3 cut(s) 216, 234, 981
Sfr303I CCGCGG 1 cut(s) 966
SfuI TTCGAA 2 cut(s) 1149, 1229
SgrBI CCGCGG 1 cut(s) 966
SmiMI CAYNNNNRTG 1 cut(s) 461
Sse9I AATT 6 cut(s) 552, 671, 683, 807, 1092, 1250
SseBI AGGCCT 1 cut(s) 153
SspMI CTAG 2 cut(s) 708, 996
StuI AGGCCT 1 cut(s) 153
StyI CCWWGG 2 cut(s) 734, 946
TaaI ACNGT 3 cut(s) 118, 238, 562
TaiI ACGT 1 cut(s) 410
TaqI TCGA 6 cut(s) 147, 192, 666, 1149, 1208, 1229
TasI AATT 6 cut(s) 552, 671, 683, 807, 1092, 1250
TauI GCSGC 3 cut(s) 80, 923, 965
TfiI GAWTC 1 cut(s) 1088
Tru1I TTAA 2 cut(s) 390, 1074
Tru9I TTAA 2 cut(s) 390, 1074
TscAI CASTG 3 cut(s) 121, 964, 981
TspDTI ATGAA 5 cut(s) 9, 74, 420, 491, 1169
TspGWI ACGGA 3 cut(s) 400, 636, 1248
TspRI CASTG 3 cut(s) 121, 964, 981
XapI RAATTY 3 cut(s) 671, 683, 1250
XmnI GAANNNNTTC 2 cut(s) 171, 810
XspI CTAG 2 cut(s) 708, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.