MD05G1349100.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009170
Physical Location & Seq
Reverse (-)
46713491 .. 46714747
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1349100.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGGCTTCCCTCCAAACTTCATCTCTTCTCTTCTCTTCTCCCTCACGTTGTTCTTCCTCAAACAGAATTCATGTCCGCAAGCTCCCAAGAGTCCGCTTCGCTGACCTGAAACAAACCACGAAGCCCGTCCTCGAAGGCCTCCAGTTGAAGGAACAAGTTCTGAAACACGAAAACACCGATTTGTATAACAACAATCACTCTGCAGCAGCAGCTCCTACAGCTTCCACAACTCAGCTCTACGCGCTCTTAGAGGCGGTGGCGGACAGAGTGGAGATGCACGCAAACATCGGCGAGCAGCGCAATAACTGGAACGCACTCTTACTTAGCTCTATCAACATGATCACTCTCTCCGCTTCCCTAATGGCCGGAGTTAATGCAGCTGCTGGAACGTCCGTTTTGGCATTGAACATTTCATCGGCTATTTTGTTTTCCGCAGCTACCGGAATGCTGCTTATCATGAACAAGATCCAGCCTTCGCAGCTCGCCGAGGAGCAGCGCAATGCTACGAGGTTGTTCAGGCAGCTCCAGAGAGAAATTGAGACGGTGTTTGCTCTTCGTGATCCTACTGAGAAAGATGTGAAGGAGACAATGGAGAAGATTTTGGCACTTGACAAAGCTTACCCACTTCCGTTGCTTGGTGCAATGCTCGAAAAATTCCCCAAAAAATTGGAGCCGGCTGTTTGGTGGCCTAGAAATGTTGTTCAATCTCAAAATCCAAGGAAACCGAAATCCCAGATGGGAAATAATGTGAATGGATGGAGTGAAGAACTTGAAAGCGAAATGAGAGAAATTATTCAAGTTGTGAAGAACAAGGACCTTGAAAACTATGAGAGATTAGGAAGCTTGGTAGTATTAAAGACCAACAAGATTTTGGCAGTTTCGGGCCCTGTGCTTACTGGGATCGCGGCTGTTGGATCCGCTTTTGTAGGCCATGGATCAGCCACTGCCGCGGTTGCAACAGTGGCTGCAGGCTCTTTGGCTAGCGTAGTGAATGCTTTTCAGCATGGCGGGCAAGTAGGGATGGTGTTTGAGATGAATAGGAACTGCGCCGGCTTCTTCAACAAGTTGGAGGATTCAATTGAAGCAACACTTGGAGAACAAGACTGGGAGAAGAGAGAAAATGGGGTGGTTTTTGAAATGAAAGTTGCAATGCAGTTGGGAAGAAGCTTGGAGCAGCTCAGAGAACTTGCATCGAAATCAGCTTTGTATCGTTCGAACGGAGAACCCATAGACGAATTCGGTTGCAAGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

419

Amino Acids

45.85

Weight (kDa)

8.3

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 78 - 394 2.5e-151 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 242, 905, 950
AclWI GGATC 6 cut(s) 460, 554, 908, 909, 922, 943
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 66, 653, 1235
AfiI CCNNNNNNNGG 2 cut(s) 148, 635
AjuI GAANNNNNNNTTGG 2 cut(s) 1074, 1106
Alw26I GTCTC 2 cut(s) 533, 578
AlwI GGATC 6 cut(s) 460, 554, 908, 909, 922, 943
AlwNI CAGNNNCTG 3 cut(s) 383, 944, 965
AoxI GGCC 5 cut(s) 136, 363, 686, 883, 928
ApaI GGGCCC 1 cut(s) 887
ApoI RAATTY 3 cut(s) 66, 653, 1235
Asp700I GAANNNNTTC 2 cut(s) 156, 792
AspLEI GCGC 4 cut(s) 244, 300, 498, 1049
AspS9I GGNCC 3 cut(s) 814, 883, 884
AsuII TTCGAA 1 cut(s) 1214
AsuNHI GCTAGC 1 cut(s) 980
AvaII GGWCC 1 cut(s) 814
BaeGI GKGCMC 1 cut(s) 887
BamHI GGATCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 887
BccI CCATC 3 cut(s) 730, 750, 1015
BclI TGATCA 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 533, 578
BfaI CTAG 2 cut(s) 690, 981
BfmI CTRYAG 3 cut(s) 201, 216, 966
Bme18I GGWCC 1 cut(s) 814
BmgT120I GGNCC 3 cut(s) 814, 883, 884
BmiI GGNNCC 3 cut(s) 672, 885, 916
BmrI ACTGGG 2 cut(s) 906, 1114
BmsI GCATC 2 cut(s) 264, 1199
BmtI GCTAGC 1 cut(s) 984
BmuI ACTGGG 2 cut(s) 906, 1114
BpmI CTGGAG 2 cut(s) 125, 509
Bpu14I TTCGAA 1 cut(s) 1214
BsaJI CCNNGG 4 cut(s) 486, 716, 931, 948
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 635
Bse118I RCCGGY 2 cut(s) 673, 1049
Bse1I ACTGG 4 cut(s) 142, 311, 901, 1109
Bse3DI GCAATG 3 cut(s) 505, 648, 1155
BseDI CCNNGG 4 cut(s) 486, 716, 931, 948
BseGI GGATG 2 cut(s) 761, 1026
BseLI CCNNNNNNNGG 2 cut(s) 148, 635
BseMI GCAATG 3 cut(s) 505, 648, 1155
BseMII CTCAG 3 cut(s) 245, 558, 1192
BseNI ACTGG 4 cut(s) 142, 311, 901, 1109
BseRI GAGGAG 1 cut(s) 503
BseSI GKGCMC 1 cut(s) 887
Bsh1236I CGCG 3 cut(s) 242, 905, 950
BshFI GGCC 5 cut(s) 138, 365, 688, 885, 930
BsiSI CCGG 4 cut(s) 366, 441, 674, 1050
BslI CCNNNNNNNGG 2 cut(s) 148, 635
BsmAI GTCTC 2 cut(s) 533, 578
BsmBI CGTCTC 1 cut(s) 533
BsmI GAATGC 2 cut(s) 450, 997
BsnI GGCC 5 cut(s) 138, 365, 688, 885, 930
Bsp119I TTCGAA 1 cut(s) 1214
Bsp120I GGGCCC 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 887
Bsp143I GATC 6 cut(s) 339, 465, 559, 900, 914, 935
Bsp19I CCATGG 1 cut(s) 931
BspANI GGCC 5 cut(s) 138, 365, 688, 885, 930
BspCNI CTCAG 3 cut(s) 244, 559, 1191
BspFNI CGCG 3 cut(s) 242, 905, 950
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 3 cut(s) 672, 885, 916
BspMAI CTGCAG 2 cut(s) 205, 970
BspOI GCTAGC 1 cut(s) 984
BspPI GGATC 6 cut(s) 460, 554, 908, 909, 922, 943
BspQI GCTCTTC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 1214
BsrDI GCAATG 3 cut(s) 505, 648, 1155
BsrFI RCCGGY 2 cut(s) 673, 1049
BsrI ACTGG 4 cut(s) 142, 311, 901, 1109
BssAI RCCGGY 2 cut(s) 673, 1049
BssECI CCNNGG 4 cut(s) 486, 716, 931, 948
BssMI GATC 6 cut(s) 339, 465, 559, 900, 914, 935
BssT1I CCWWGG 2 cut(s) 716, 931
Bst4CI ACNGT 2 cut(s) 544, 961
Bst6I CTCTTC 5 cut(s) 30, 35, 40, 558, 1106
BstBI TTCGAA 1 cut(s) 1214
BstDEI CTNAG 5 cut(s) 231, 247, 323, 567, 1178
BstDSI CCRYGG 2 cut(s) 931, 948
BstF5I GGATG 2 cut(s) 761, 1026
BstFNI CGCG 3 cut(s) 242, 905, 950
BstHHI GCGC 4 cut(s) 244, 300, 498, 1049
BstKTI GATC 6 cut(s) 342, 468, 562, 903, 917, 938
BstMAI GTCTC 2 cut(s) 533, 578
BstMBI GATC 6 cut(s) 339, 465, 559, 900, 914, 935
BstMWI GCNNNNNNNGC 9 cut(s) 209, 218, 241, 297, 478, 947, 953, 962, 1009
BstSFI CTRYAG 3 cut(s) 201, 216, 966
BstSLI GKGCMC 1 cut(s) 887
BstUI CGCG 3 cut(s) 242, 905, 950
BstX2I RGATCY 2 cut(s) 465, 914
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 2 cut(s) 465, 914
BsuRI GGCC 5 cut(s) 138, 365, 688, 885, 930
BtgI CCRYGG 2 cut(s) 931, 948
BtsCI GGATG 2 cut(s) 761, 1026
BtsI GCAGTG 1 cut(s) 942
BtsIMutI CAGTG 2 cut(s) 942, 966
CaiI CAGNNNCTG 3 cut(s) 383, 944, 965
CciI TCATGA 1 cut(s) 456
CfoI GCGC 4 cut(s) 244, 300, 498, 1049
Cfr10I RCCGGY 2 cut(s) 673, 1049
Cfr13I GGNCC 3 cut(s) 814, 883, 884
Cfr42I CCGCGG 1 cut(s) 951
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 5 cut(s) 71, 337, 457, 932, 1004
DdeI CTNAG 5 cut(s) 231, 247, 323, 567, 1178
DpnI GATC 6 cut(s) 341, 467, 561, 902, 916, 937
DpnII GATC 6 cut(s) 339, 465, 559, 900, 914, 935
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 5 cut(s) 30, 35, 40, 558, 1106
EarI CTCTTC 5 cut(s) 30, 35, 40, 558, 1106
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 716, 931
Eco147I AGGCCT 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 887
Eco47I GGWCC 1 cut(s) 814
EcoO109I RGGNCCY 2 cut(s) 814, 884
EcoRI GAATTC 2 cut(s) 66, 1235
EcoT14I CCWWGG 2 cut(s) 716, 931
EcoT38I GRGCYC 1 cut(s) 887
ErhI CCWWGG 2 cut(s) 716, 931
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 5 cut(s) 74, 340, 460, 935, 1007
FaiI YATR 8 cut(s) 72, 186, 338, 458, 828, 933, 1005, 1229
FalI AAGNNNNNCTT 2 cut(s) 1074, 1106
FatI CATG 5 cut(s) 70, 336, 456, 931, 1003
FauI CCCGC 1 cut(s) 1001
FbaI TGATCA 1 cut(s) 339
FokI GGATG 2 cut(s) 768, 1033
FriOI GRGCYC 1 cut(s) 887
FspBI CTAG 2 cut(s) 690, 981
GlaI GCGC 4 cut(s) 243, 299, 497, 1048
GsuI CTGGAG 2 cut(s) 125, 509
HaeIII GGCC 5 cut(s) 138, 365, 688, 885, 930
HapII CCGG 4 cut(s) 366, 441, 674, 1050
HhaI GCGC 4 cut(s) 244, 300, 498, 1049
Hin1II CATG 5 cut(s) 74, 340, 460, 935, 1007
Hin6I GCGC 4 cut(s) 242, 298, 496, 1047
HinP1I GCGC 4 cut(s) 242, 298, 496, 1047
HindIII AAGCTT 4 cut(s) 615, 841, 1165, 1247
HinfI GANTC 2 cut(s) 90, 1073
HpaII CCGG 4 cut(s) 366, 441, 674, 1050
Hpy188I TCNGA 2 cut(s) 162, 1181
Hpy188III TCNNGA 3 cut(s) 457, 526, 557
HpyAV CCTTC 4 cut(s) 128, 142, 483, 574
HpyCH4III ACNGT 2 cut(s) 544, 961
HpyCH4IV ACGT 2 cut(s) 46, 389
HpyF10VI GCNNNNNNNGC 9 cut(s) 209, 218, 241, 297, 478, 947, 953, 962, 1009
HpyF3I CTNAG 5 cut(s) 231, 247, 323, 567, 1178
HpySE526I ACGT 2 cut(s) 46, 389
Hsp92II CATG 5 cut(s) 74, 340, 460, 935, 1007
HspAI GCGC 4 cut(s) 242, 298, 496, 1047
KroI GCCGGC 2 cut(s) 673, 1049
KroNI GCCGGC 2 cut(s) 675, 1051
Ksp22I TGATCA 1 cut(s) 339
KspI CCGCGG 1 cut(s) 951
Kzo9I GATC 6 cut(s) 339, 465, 559, 900, 914, 935
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 6 cut(s) 87, 217, 490, 528, 670, 1171
LweI GCATC 2 cut(s) 264, 1199
MaeI CTAG 2 cut(s) 690, 981
MaeII ACGT 2 cut(s) 46, 389
MalI GATC 6 cut(s) 341, 467, 561, 902, 916, 937
MboI GATC 6 cut(s) 339, 465, 559, 900, 914, 935
MfeI CAATTG 1 cut(s) 1077
MflI RGATCY 2 cut(s) 465, 914
MhlI GDGCHC 1 cut(s) 887
MluCI AATT 7 cut(s) 66, 534, 653, 665, 789, 1077, 1235
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 892, 1047
MnlI CCTC 9 cut(s) 20, 52, 67, 140, 149, 244, 481, 501, 1063
MroNI GCCGGC 2 cut(s) 673, 1049
MroXI GAANNNNTTC 2 cut(s) 156, 792
MseI TTAA 2 cut(s) 372, 854
MspA1I CMGCKG 2 cut(s) 380, 950
MspI CCGG 4 cut(s) 366, 441, 674, 1050
MunI CAATTG 1 cut(s) 1077
Mva1269I GAATGC 2 cut(s) 450, 997
MvnI CGCG 3 cut(s) 242, 905, 950
MwoI GCNNNNNNNGC 9 cut(s) 209, 218, 241, 297, 478, 947, 953, 962, 1009
NaeI GCCGGC 2 cut(s) 675, 1051
NcoI CCATGG 1 cut(s) 931
NdeII GATC 6 cut(s) 339, 465, 559, 900, 914, 935
NgoMIV GCCGGC 2 cut(s) 673, 1049
NheI GCTAGC 1 cut(s) 980
NlaIII CATG 5 cut(s) 74, 340, 460, 935, 1007
NlaIV GGNNCC 3 cut(s) 672, 885, 916
NmeAIII GCCGAG 1 cut(s) 511
NspV TTCGAA 1 cut(s) 1214
PagI TCATGA 1 cut(s) 456
PceI AGGCCT 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 2 cut(s) 450, 997
PdiI GCCGGC 2 cut(s) 675, 1051
PdmI GAANNNNTTC 2 cut(s) 156, 792
PfeI GAWTC 1 cut(s) 1073
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 814
PspN4I GGNNCC 3 cut(s) 672, 885, 916
PspOMI GGGCCC 1 cut(s) 883
PspPI GGNCC 3 cut(s) 814, 883, 884
PspPPI RGGWCCY 1 cut(s) 814
PstI CTGCAG 2 cut(s) 205, 970
PstNI CAGNNNCTG 3 cut(s) 383, 944, 965
PsuI RGATCY 2 cut(s) 465, 914
PvuII CAGCTG 1 cut(s) 380
SacII CCGCGG 1 cut(s) 951
SapI GCTCTTC 1 cut(s) 558
SaqAI TTAA 2 cut(s) 372, 854
Sau3AI GATC 6 cut(s) 339, 465, 559, 900, 914, 935
Sau96I GGNCC 3 cut(s) 814, 883, 884
SchI GAGTC 1 cut(s) 99
SduI GDGCHC 1 cut(s) 887
SfaNI GCATC 2 cut(s) 264, 1199
SfcI CTRYAG 3 cut(s) 201, 216, 966
Sfr303I CCGCGG 1 cut(s) 951
SfuI TTCGAA 1 cut(s) 1214
SgrBI CCGCGG 1 cut(s) 951
SinI GGWCC 1 cut(s) 814
Sse9I AATT 7 cut(s) 66, 534, 653, 665, 789, 1077, 1235
SseBI AGGCCT 1 cut(s) 138
SspMI CTAG 2 cut(s) 690, 981
StuI AGGCCT 1 cut(s) 138
StyI CCWWGG 2 cut(s) 716, 931
TaaI ACNGT 2 cut(s) 544, 961
TaiI ACGT 2 cut(s) 49, 392
TaqI TCGA 4 cut(s) 132, 648, 1193, 1214
TasI AATT 7 cut(s) 66, 534, 653, 665, 789, 1077, 1235
TauI GCSGC 2 cut(s) 908, 950
TfiI GAWTC 1 cut(s) 1073
Tru1I TTAA 2 cut(s) 372, 854
Tru9I TTAA 2 cut(s) 372, 854
TscAI CASTG 2 cut(s) 949, 966
TspDTI ATGAA 6 cut(s) 9, 59, 402, 473, 1049, 1154
TspGWI ACGGA 3 cut(s) 382, 618, 1233
TspRI CASTG 2 cut(s) 949, 966
VpaK11BI GGWCC 1 cut(s) 814
XapI RAATTY 3 cut(s) 66, 653, 1235
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmnI GAANNNNTTC 2 cut(s) 156, 792
XspI CTAG 2 cut(s) 690, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.