AT4G04670

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006279
Physical Location & Seq
Forward (+)
2367434 .. 2372220
4787 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G04670.1

Sequence Viewer

Length: 2988 bp
ATGGATTTCGAGAAGAGAAAAGCTGCGACACTTGCTTCAATTCGTTCATCAGTGACTGATAAATCTCCCAAAGGTTTTCTCGACGAACCCATCATCCCTCTCCTCGAAACCATCAATCATCACCCATCTTACTTCACTACCAGTTCCTGCTCAGGTCGAATCTCAATCCTTTCTCAACCCAAACCAAAATCGAATGATTCAACTAAGAAGAAAGCTCGTGGCGGTTCATGGCTCTATATCACACACGACCCAGCTGATTCGGATTTGGTAATTTCTCTCCTTTTCCCTTCCAAGTCAAATCAGATTGACCCAATTGACCAACCTAGCGAATTAGTCTTCCGATTCGAGCCGTTGATCATTGCTGTCGAATGTAAGGATTTGGGTTCGGCTCAGTTTCTTGTGGCGTTGGCTATATCAGCTGGGTTTAGAGAGTCTGGTATCACATCTTGTGGTGATGGCAAAAGGGTAATTATAGCTATACGATGCTCCATTCGAATGGAGGTTCCTATTGGTGATACAGAGAAGCTTATGGTGTCACCAGAGTATGTGAAGTTCCTTGTTGATATTGCTAATGAGAAGATGGATGCTAATAGGAAGCGAACCGATGGGTTTAGTGTAGCTTTGGCTAGTAATGGTTTCAAGAATCCAGATGAAAATGATGTTGATGAAGATGATAATTACGAGAATCTAGCTGCAAATCATGACTCAAGTATCAACAACGGAAACTTGTATCCTGGGGTTCAGAAAGAACTTATACCACTCGAGAAATTATCCATTGTTGGGGAACCTGTTGAGAAGCTTCACCTTTGGGGACACTCAGCTTGTACGATAGATGAATCAGACCGGAAAGAGGTTATCGTGTTTGGTGGGTTTGGAGGTTTTGGTAGACATGCTCGAAGAAACGAGTCTTTGTTGCTCAATCCTTCATGTGGCACCTTAAAGTTGATTGCTGTCAATGAATCTCCATCGGCACGACTTGGCCACACAGCTTCAATGGTTGGCGATTTCATGTTTGTAATTGGAGGAAGGGCTGATCCCTTGAACATTTTGAATGATGTGTGGAGGCTCGATATATCCACGGGTGAATGGAGCTCACAGAGGTGTGTTGGAAGTGAATTTCCTCCAAGGCATCGACATGCAGCAGCCAGTGTTGGCACAAAAGTATACATATTCGGTGGGCTTTACAATGATAAGATAGTTTCCTCTATGCACATTTTGGATACAAAGGACCTTCAGTGGAAAGAAGTTGAGCAACAAGGTCAATGGCCCTGTGCGCGCCATTCGCATGCTATGGTTGCATATGGGTCGCAGTCATTCATGTTTGGAGGATACAATGGGGAAAATGTACTCAATGATCTATACAGCTTTGATGTCCAAAGCTGTTCTTGGAAACTTGAAGTGATTTCTGGAAAATGGCCTCATGCCAGGTTTTCACACTCAATGTTTGTTTATAAGCATACCATTGGCATAATTGGAGGTTGTCCTGTCTCACAAAATTGTCAAGAGTTGACTTTGCTAGATCTGAAGCACCGATTGTGGAGGAGTGTGAGATTGGAATTTATGAACAAAGAGTTGTTTGTTAGAAGTACAGCCAGCATTCTTGGTGATGATCTTATCGTCATTGGGGGTGGGGCTGCTTGCTATGCTTTTGGGACAAAGTTTAGTGAACCAGTGAAAATCAACTTGGTTCAATCTGTGACTATGAGTGAAAATCATCTTCCTCCCCAACCTGAAGATGTTTCTCTAGAATCGAATAAAAACAATGCAGATTTGAAGACTGAGACGTCTCTGTCTCAACCATGGGTGATACAGCTAGAAAGAAAGTATGCAAAGTTCGGCAAGGACATACTTAAAAGTTTCGGATGGTTAGACCTTGAGAGAAAGGTTTACTCAAACGAAAAAGGTCTTTGTATATGCTTTCCTGTGACTGAAAACTTTTCTGAACTTTTCCATGAGAAGCAGCTATTGGGCAAGGACTTTGAAAGGTCAGAGGAAAATAATCTAACAAAGGGTCTATCGTTAAAAGATATATCCTGCTCAGCGGCTTTGAATCTATTGAAGGAACATGGTGCCAAAAAACTCATTAATGTAGCTTTTGAAGCCAAAAAAGTTGCAAAATCCCCCTTACAGAGAATGAGAGAAGATATCACATCTATACTTAAACAGAAAGGCCTACCAGAAGAGCTTTTGGATGAGCTCCCACAGAAATGGGAACGGCTTGGGGATATTGTTGTGGTTCCTGCAACATCCTTTAAAGATCCAACTTGGAGCTCTATCAATGATGAAGTTTGGTGTGCTGTTTCTAAATCGCTTAGTGCCAACCGCCTTGCACGCCAAGGACGAGTTGAACCTAATGGTACAAGGGATAGTACATTGGAGATTCTTGTGGGAGACAATGGATGGGTTAATCACCGTGAAAATGGGATCTTGTACTCTTTCGATGCTACGAAGTGTATGTTCTCATGGGGTAATCTCTCAGAAAAGCTTCGTATGGGTAACATGGCCTGTGAAAATGAAGTTGTCGTGGACCTGTTTGCTGGAATTGGATATTTTGTTCTCCCATTTCTTGTGAGGGCAAAAGCAAAGCTAGTATATGCTTGTGAGTGGAATCCCCATGCTATTGAAGCTCTTCGACGTAATGTTGAAGCAAATTCTGTTTCTGAGCGTTGTATCATTCTTGAAGGGGATAACCGGATCACCGCACCAAAAGGAGTAGCTGACAGAGTCAATCTTGGTCTCATCCCGAGTAGCGAAGGAAGCTGGGTCACAGCTATTCAGGCATTAAGGCCTGAGGGAGGAATACTCCATGTGCACGGAAACGTCAAGGACTCAGATGAGAGCAGTTGGGGCGAGCATGTCACCAAAACATTAAGTGATATCGCCAGAGCCGAAGGTCGTAGCTGGGAAGTTACAGTGGAACATATAGAGAAGGTGAAATGGTACGCTCCTCGGATTCGCCATCTTGTTGCCGATGTGAGATGCAGATGA

Protein Analysis

995

Amino Acids

110.85

Weight (kDa)

6.23

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 209 1.4e-65 Methyltransferase TYW3
Beta-prop_ATRN-LZTR1 PF24981 268 - 344 1.8e-06 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 270 - 443 1.6e-37 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 282 - 413 3.6e-22 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 310 - 419 4.7e-07 FKB95, Kelch-repeats domain
Beta-prop_TYW4 PF13418 322 - 554 4.5e-26 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 324 - 365 8.2e-07 Kelch motif
Kelch_HCF PF13854 350 - 396 2e-06 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 351 - 418 5e-09 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 375 - 418 1e-05 Kelch motif
Kelch_1 PF01344 375 - 416 8e-08 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 379 - 548 1.5e-35 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 397 - 513 2e-20 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 406 - 465 3.7e-12 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 409 - 499 8.1e-10 Host cell factor, Kelch-repeats domain
TYW2_N_2 PF25133 718 - 799 5.1e-16 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 804 - 943 1.8e-33 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1452
AasI GACNNNNNNGTC 2 cut(s) 1783, 1789
AatII GACGTC 1 cut(s) 1787
AccB1I GGYRCC 2 cut(s) 932, 2069
AccI GTMKAC 2 cut(s) 886, 1164
AccII CGCG 1 cut(s) 1276
AciI CCGC 4 cut(s) 222, 2042, 2323, 2700
AclWI GGATC 4 cut(s) 1028, 2252, 2432, 2702
AcoI YGGCCR 1 cut(s) 979
AcsI RAATTY 3 cut(s) 1115, 1556, 2650
AcuI CTGAAG 3 cut(s) 1217, 1544, 1752
AcyI GRCGYC 1 cut(s) 1784
AfaI GTAC 7 cut(s) 826, 1347, 1588, 2359, 2371, 2432, 2942
AfiI CCNNNNNNNGG 4 cut(s) 780, 850, 929, 2795
AjnI CCWGG 2 cut(s) 733, 1424
AjuI GAANNNNNNNTTGG 4 cut(s) 1667, 1699, 1949, 1981
AleI CACNNNNGTG 1 cut(s) 1099
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 2538, 2570
Alw21I GWGCWC 4 cut(s) 1094, 2199, 2273, 2814
Alw26I GTCTC 6 cut(s) 1492, 1775, 1791, 1797, 2385, 2741
Alw44I GTGCAC 1 cut(s) 2810
AlwI GGATC 4 cut(s) 1028, 2252, 2432, 2702
AlwNI CAGNNNCTG 2 cut(s) 56, 147
Ama87I CYCGRG 2 cut(s) 761, 2743
AoxI GGCC 6 cut(s) 979, 1265, 1415, 2170, 2502, 2786
ApaLI GTGCAC 1 cut(s) 2810
ApeKI GCWGC 6 cut(s) 23, 692, 1139, 1142, 1634, 1960
ApoI RAATTY 3 cut(s) 1115, 1556, 2650
AseI ATTAAT 1 cut(s) 2085
Asp700I GAANNNNTTC 2 cut(s) 2484, 2628
AspLEI GCGC 2 cut(s) 1276, 1278
AspS9I GGNCC 3 cut(s) 1228, 1266, 2527
AsuII TTCGAA 1 cut(s) 493
AvaI CYCGRG 2 cut(s) 761, 2743
AvaII GGWCC 2 cut(s) 1228, 2527
AxyI CCTNAGG 1 cut(s) 2790
BaeGI GKGCMC 1 cut(s) 2814
BaeI ACNNNNGTAYC 2 cut(s) 694, 727
BalI TGGCCA 1 cut(s) 981
BanI GGYRCC 2 cut(s) 932, 2069
BanII GRGCYC 3 cut(s) 1094, 2199, 2273
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 2 cut(s) 328, 1781
Bbv12I GWGCWC 4 cut(s) 1094, 2199, 2273, 2814
BbvI GCAGC 6 cut(s) 10, 679, 1151, 1154, 1621, 1972
BceAI ACGGC 2 cut(s) 334, 2231
BcgI CGANNNNNNTGC 2 cut(s) 1287, 1321
BciT130I CCWGG 2 cut(s) 735, 1426
BciVI GTATCC 3 cut(s) 741, 1213, 1322
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 6 cut(s) 1492, 1775, 1791, 1797, 2385, 2741
BfaI CTAG 7 cut(s) 324, 627, 689, 1517, 1745, 1814, 2588
BfuI GTATCC 3 cut(s) 741, 1213, 1322
BglII AGATCT 1 cut(s) 1519
BisI GCNGC 7 cut(s) 24, 693, 1140, 1143, 1635, 1961, 2043
BlpI GCTNAGC 1 cut(s) 2038
BlsI GCNGC 7 cut(s) 25, 694, 1141, 1144, 1636, 1962, 2044
Bme1390I CCNGG 2 cut(s) 735, 1426
Bme18I GGWCC 2 cut(s) 1228, 2527
BmeT110I CYCGRG 2 cut(s) 761, 2743
BmgT120I GGNCC 3 cut(s) 1228, 1266, 2527
BmiI GGNNCC 5 cut(s) 504, 786, 934, 2071, 2238
BmrFI CCNGG 2 cut(s) 735, 1426
BmsI GCATC 5 cut(s) 473, 574, 1138, 2431, 2969
BpiI GAAGAC 2 cut(s) 328, 1781
BplI GAGNNNNNCTC 2 cut(s) 2787, 2819
Bpu10I CCTNAGC 1 cut(s) 151
Bpu1102I GCTNAGC 1 cut(s) 2038
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 2 cut(s) 691, 1895
BsaHI GRCGYC 1 cut(s) 1784
BsaI GGTCTC 1 cut(s) 2741
BsaJI CCNNGG 6 cut(s) 734, 1077, 1124, 1799, 2335, 2948
BsaWI WCCGGW 2 cut(s) 843, 2691
Bsc4I CCNNNNNNNGG 4 cut(s) 780, 850, 929, 2795
Bse1I ACTGG 3 cut(s) 141, 1146, 1670
Bse21I CCTNAGG 1 cut(s) 2790
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 2 cut(s) 735, 1426
BseDI CCNNGG 6 cut(s) 734, 1077, 1124, 1799, 2335, 2948
BseGI GGATG 7 cut(s) 93, 589, 1868, 2197, 2246, 2405, 2739
BseLI CCNNNNNNNGG 4 cut(s) 780, 850, 929, 2795
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 9 cut(s) 165, 404, 831, 1770, 2052, 2490, 2652, 2781, 2844
BseNI ACTGG 3 cut(s) 141, 1146, 1670
BsePI GCGCGC 1 cut(s) 1274
BseRI GAGGAG 3 cut(s) 92, 1555, 2937
BseSI GKGCMC 1 cut(s) 2814
BseXI GCAGC 6 cut(s) 10, 679, 1151, 1154, 1621, 1972
BseYI CCCAGC 4 cut(s) 250, 419, 2760, 2901
Bsh1236I CGCG 1 cut(s) 1276
BshFI GGCC 6 cut(s) 981, 1267, 1417, 2172, 2504, 2788
BshNI GGYRCC 2 cut(s) 932, 2069
BsiHKAI GWGCWC 4 cut(s) 1094, 2199, 2273, 2814
BsiHKCI CYCGRG 2 cut(s) 761, 2743
BsiSI CCGG 2 cut(s) 844, 2692
BslFI GGGAC 2 cut(s) 825, 1666
BslI CCNNNNNNNGG 4 cut(s) 780, 850, 929, 2795
BsmAI GTCTC 6 cut(s) 1492, 1775, 1791, 1797, 2385, 2741
BsmBI CGTCTC 2 cut(s) 1775, 1791
BsmFI GGGAC 2 cut(s) 825, 1666
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 6 cut(s) 981, 1267, 1417, 2172, 2504, 2788
Bso31I GGTCTC 1 cut(s) 2741
BsoBI CYCGRG 2 cut(s) 761, 2743
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 4 cut(s) 1094, 2199, 2273, 2814
Bsp143I GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
Bsp1720I GCTNAGC 1 cut(s) 2038
Bsp19I CCATGG 1 cut(s) 1799
BspACI CCGC 4 cut(s) 222, 2042, 2323, 2700
BspANI GGCC 6 cut(s) 981, 1267, 1417, 2172, 2504, 2788
BspCNI CTCAG 9 cut(s) 164, 403, 830, 1771, 2051, 2489, 2653, 2782, 2843
BspFNI CGCG 1 cut(s) 1276
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 5 cut(s) 504, 786, 934, 2071, 2238
BspPI GGATC 4 cut(s) 1028, 2252, 2432, 2702
BspQI GCTCTTC 2 cut(s) 2175, 2634
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 2 cut(s) 932, 2069
BspTNI GGTCTC 1 cut(s) 2741
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 3 cut(s) 141, 1146, 1670
BssECI CCNNGG 6 cut(s) 734, 1077, 1124, 1799, 2335, 2948
BssHII GCGCGC 1 cut(s) 1274
BssMI GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
BssNAI GTATAC 1 cut(s) 1165
BssNI GRCGYC 1 cut(s) 1784
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 3 cut(s) 1124, 1799, 2335
Bst1107I GTATAC 1 cut(s) 1165
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 735, 1426
Bst4CI ACNGT 2 cut(s) 2414, 2914
Bst6I CTCTTC 3 cut(s) 8, 2175, 2634
BstACI GRCGYC 1 cut(s) 1784
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 6 cut(s) 1276, 1287, 1594, 1639, 2332, 2852
BstDSI CCRYGG 2 cut(s) 1077, 1799
BstENI CCTNNNNNAGG 1 cut(s) 2793
BstF5I GGATG 7 cut(s) 93, 589, 1868, 2197, 2246, 2405, 2739
BstFNI CGCG 1 cut(s) 1276
BstHHI GCGC 2 cut(s) 1276, 1278
BstKTI GATC 8 cut(s) 357, 1036, 1357, 1522, 1612, 2260, 2427, 2697
BstMAI GTCTC 6 cut(s) 1492, 1775, 1791, 1797, 2385, 2741
BstMBI GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
BstNI CCWGG 2 cut(s) 735, 1426
BstNSI RCATGY 4 cut(s) 893, 1139, 1289, 2858
BstSCI CCNGG 2 cut(s) 733, 1424
BstSLI GKGCMC 1 cut(s) 2814
BstUI CGCG 1 cut(s) 1276
BstV1I GCAGC 6 cut(s) 10, 679, 1151, 1154, 1621, 1972
BstV2I GAAGAC 2 cut(s) 328, 1781
BstX2I RGATCY 3 cut(s) 1519, 2257, 2424
BstXI CCANNNNNNTGG 2 cut(s) 496, 2208
BstYI RGATCY 3 cut(s) 1519, 2257, 2424
BstZ17I GTATAC 1 cut(s) 1165
Bsu36I CCTNAGG 1 cut(s) 2790
BsuI GTATCC 3 cut(s) 741, 1213, 1322
BsuRI GGCC 6 cut(s) 981, 1267, 1417, 2172, 2504, 2788
BtgI CCRYGG 2 cut(s) 1077, 1799
BtsCI GGATG 7 cut(s) 93, 589, 1868, 2197, 2246, 2405, 2739
BtsIMutI CAGTG 5 cut(s) 57, 1153, 1241, 1677, 2919
Cac8I GCNNGC 6 cut(s) 1276, 1287, 1594, 1639, 2332, 2852
CaiI CAGNNNCTG 2 cut(s) 56, 147
CciI TCATGA 1 cut(s) 700
CfoI GCGC 2 cut(s) 1276, 1278
Cfr13I GGNCC 3 cut(s) 1228, 1266, 2527
Csp6I GTAC 7 cut(s) 825, 1346, 1587, 2358, 2370, 2431, 2941
CviQI GTAC 7 cut(s) 825, 1346, 1587, 2358, 2370, 2431, 2941
DpnI GATC 8 cut(s) 356, 1035, 1356, 1521, 1611, 2259, 2426, 2696
DpnII GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
DraI TTTAAA 1 cut(s) 2254
DrdI GACNNNNNNGTC 2 cut(s) 1783, 1789
DseDI GACNNNNNNGTC 2 cut(s) 1783, 1789
EaeI YGGCCR 1 cut(s) 979
Eam1104I CTCTTC 3 cut(s) 8, 2175, 2634
EarI CTCTTC 3 cut(s) 8, 2175, 2634
Ecl136II GAGCTC 3 cut(s) 1092, 2197, 2271
Eco130I CCWWGG 3 cut(s) 1124, 1799, 2335
Eco147I AGGCCT 2 cut(s) 2172, 2788
Eco24I GRGCYC 3 cut(s) 1094, 2199, 2273
Eco31I GGTCTC 1 cut(s) 2741
Eco32I GATATC 2 cut(s) 2146, 2878
Eco47I GGWCC 2 cut(s) 1228, 2527
Eco53kI GAGCTC 3 cut(s) 1092, 2197, 2271
Eco57I CTGAAG 3 cut(s) 1217, 1544, 1752
Eco81I CCTNAGG 1 cut(s) 2790
Eco88I CYCGRG 2 cut(s) 761, 2743
EcoICRI GAGCTC 3 cut(s) 1092, 2197, 2271
EcoNI CCTNNNNNAGG 1 cut(s) 2793
EcoO109I RGGNCCY 1 cut(s) 1228
EcoRII CCWGG 2 cut(s) 733, 1424
EcoRV GATATC 2 cut(s) 2146, 2878
EcoT14I CCWWGG 3 cut(s) 1124, 1799, 2335
EcoT38I GRGCYC 3 cut(s) 1094, 2199, 2273
ErhI CCWWGG 3 cut(s) 1124, 1799, 2335
Esp3I CGTCTC 2 cut(s) 1775, 1791
FalI AAGNNNNNCTT 2 cut(s) 1369, 1401
FaqI GGGAC 2 cut(s) 825, 1666
FauNDI CATATG 1 cut(s) 1300
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 2 cut(s) 886, 1164
Fnu4HI GCNGC 7 cut(s) 24, 693, 1140, 1143, 1635, 1961, 2043
FokI GGATG 7 cut(s) 80, 596, 1875, 2204, 2233, 2412, 2726
FriOI GRGCYC 3 cut(s) 1094, 2199, 2273
Fsp4HI GCNGC 7 cut(s) 24, 693, 1140, 1143, 1635, 1961, 2043
FspBI CTAG 7 cut(s) 324, 627, 689, 1517, 1745, 1814, 2588
GlaI GCGC 2 cut(s) 1275, 1277
GluI GCNGC 7 cut(s) 24, 693, 1140, 1143, 1635, 1961, 2043
GsaI CCCAGC 4 cut(s) 254, 423, 2764, 2905
HaeIII GGCC 6 cut(s) 981, 1267, 1417, 2172, 2504, 2788
HapII CCGG 2 cut(s) 844, 2692
HhaI GCGC 2 cut(s) 1276, 1278
Hin1I GRCGYC 1 cut(s) 1784
Hin6I GCGC 2 cut(s) 1274, 1276
HinP1I GCGC 2 cut(s) 1274, 1276
HincII GTYRAC 1 cut(s) 1509
HindII GTYRAC 1 cut(s) 1509
HindIII AAGCTT 3 cut(s) 524, 797, 2483
HpaII CCGG 2 cut(s) 844, 2692
Hpy166II GTNNAC 7 cut(s) 887, 1165, 1509, 1667, 1888, 2527, 2812
Hpy8I GTNNAC 7 cut(s) 887, 1165, 1509, 1667, 1888, 2527, 2812
Hpy99I CGWCG 2 cut(s) 86, 2637
HpyAV CCTTC 9 cut(s) 297, 933, 1020, 1241, 2053, 2675, 2747, 2885, 2923
HpyCH4III ACNGT 2 cut(s) 2414, 2914
HpyCH4IV ACGT 3 cut(s) 1784, 2635, 2820
HpySE526I ACGT 3 cut(s) 1784, 2635, 2820
Hsp92I GRCGYC 1 cut(s) 1784
HspAI GCGC 2 cut(s) 1274, 1276
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
LguI GCTCTTC 2 cut(s) 2175, 2634
LmnI GCTCC 5 cut(s) 491, 1089, 2202, 2268, 2950
Lsp1109I GCAGC 6 cut(s) 10, 679, 1151, 1154, 1621, 1972
LweI GCATC 5 cut(s) 473, 574, 1138, 2431, 2969
MaeI CTAG 7 cut(s) 324, 627, 689, 1517, 1745, 1814, 2588
MaeII ACGT 3 cut(s) 1784, 2635, 2820
MaeIII GTNAC 8 cut(s) 52, 534, 1696, 1924, 2495, 2764, 2857, 2908
MalI GATC 8 cut(s) 356, 1035, 1356, 1521, 1611, 2259, 2426, 2696
MboI GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 3 cut(s) 1519, 2257, 2424
MhlI GDGCHC 4 cut(s) 1094, 2199, 2273, 2814
MlsI TGGCCA 1 cut(s) 981
MluNI TGGCCA 1 cut(s) 981
MlyI GAGTC 5 cut(s) 440, 698, 914, 2733, 2822
MmeI TCCRAC 2 cut(s) 1087, 2285
Mox20I TGGCCA 1 cut(s) 981
MroXI GAANNNNTTC 2 cut(s) 2484, 2628
MscI TGGCCA 1 cut(s) 981
MseI TTAA 9 cut(s) 938, 1851, 2021, 2085, 2160, 2253, 2406, 2783, 2870
MslI CAYNNNNRTG 5 cut(s) 494, 1099, 1134, 1284, 2206
Msp20I TGGCCA 1 cut(s) 981
MspA1I CMGCKG 3 cut(s) 254, 419, 2042
MspI CCGG 2 cut(s) 844, 2692
MspR9I CCNGG 2 cut(s) 735, 1426
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 2 cut(s) 735, 1426
MvnI CGCG 1 cut(s) 1276
NcoI CCATGG 1 cut(s) 1799
NdeI CATATG 1 cut(s) 1300
NdeII GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
NlaIV GGNNCC 5 cut(s) 504, 786, 934, 2071, 2238
NmuCI GTSAC 6 cut(s) 52, 534, 1696, 1924, 2764, 2857
NspI RCATGY 4 cut(s) 893, 1139, 1289, 2858
NspV TTCGAA 1 cut(s) 493
OliI CACNNNNGTG 1 cut(s) 1099
PaeI GCATGC 1 cut(s) 1289
PaeR7I CTCGAG 1 cut(s) 761
PagI TCATGA 1 cut(s) 700
PauI GCGCGC 1 cut(s) 1274
PceI AGGCCT 2 cut(s) 2172, 2788
PciSI GCTCTTC 2 cut(s) 2175, 2634
PcsI WCGNNNNNNNCGW 2 cut(s) 2338, 2886
PctI GAATGC 1 cut(s) 1596
PdmI GAANNNNTTC 2 cut(s) 2484, 2628
PflFI GACNNNGTC 1 cut(s) 2723
PkrI GCNGC 7 cut(s) 25, 694, 1141, 1144, 1636, 1962, 2044
PleI GAGTC 5 cut(s) 439, 698, 913, 2732, 2822
PpsI GAGTC 5 cut(s) 439, 698, 913, 2732, 2822
PpuMI RGGWCCY 1 cut(s) 1228
PshBI ATTAAT 1 cut(s) 2085
PsiI TTATAA 1 cut(s) 1452
Psp124BI GAGCTC 3 cut(s) 1094, 2199, 2273
Psp5II RGGWCCY 1 cut(s) 1228
Psp6I CCWGG 2 cut(s) 733, 1424
PspFI CCCAGC 4 cut(s) 250, 419, 2760, 2901
PspGI CCWGG 2 cut(s) 733, 1424
PspN4I GGNNCC 5 cut(s) 504, 786, 934, 2071, 2238
PspPI GGNCC 3 cut(s) 1228, 1266, 2527
PspPPI RGGWCCY 1 cut(s) 1228
PsrI GAACNNNNNNTAC 4 cut(s) 536, 568, 1817, 1849
PstNI CAGNNNCTG 2 cut(s) 56, 147
PsuI RGATCY 3 cut(s) 1519, 2257, 2424
PsyI GACNNNGTC 1 cut(s) 2723
PteI GCGCGC 1 cut(s) 1274
PvuII CAGCTG 2 cut(s) 254, 419
RsaI GTAC 7 cut(s) 826, 1347, 1588, 2359, 2371, 2432, 2942
RsaNI GTAC 7 cut(s) 825, 1346, 1587, 2358, 2370, 2431, 2941
RseI CAYNNNNRTG 5 cut(s) 494, 1099, 1134, 1284, 2206
SacI GAGCTC 3 cut(s) 1094, 2199, 2273
SapI GCTCTTC 2 cut(s) 2175, 2634
SaqAI TTAA 9 cut(s) 938, 1851, 2021, 2085, 2160, 2253, 2406, 2783, 2870
SatI GCNGC 7 cut(s) 24, 693, 1140, 1143, 1635, 1961, 2043
Sau3AI GATC 8 cut(s) 354, 1033, 1354, 1519, 1609, 2257, 2424, 2694
Sau96I GGNCC 3 cut(s) 1228, 1266, 2527
SchI GAGTC 5 cut(s) 440, 698, 914, 2733, 2822
ScrFI CCNGG 2 cut(s) 735, 1426
SduI GDGCHC 4 cut(s) 1094, 2199, 2273, 2814
SfaNI GCATC 5 cut(s) 473, 574, 1138, 2431, 2969
Sfr274I CTCGAG 1 cut(s) 761
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 2 cut(s) 1228, 2527
SlaI CTCGAG 1 cut(s) 761
SmiMI CAYNNNNRTG 5 cut(s) 494, 1099, 1134, 1284, 2206
SmlI CTYRAG 3 cut(s) 706, 761, 1874
SmoI CTYRAG 3 cut(s) 706, 761, 1874
SphI GCATGC 1 cut(s) 1289
SseBI AGGCCT 2 cut(s) 2172, 2788
SsiI CCGC 4 cut(s) 222, 2042, 2323, 2700
SspMI CTAG 7 cut(s) 324, 627, 689, 1517, 1745, 1814, 2588
SstI GAGCTC 3 cut(s) 1094, 2199, 2273
StuI AGGCCT 2 cut(s) 2172, 2788
StyD4I CCNGG 2 cut(s) 733, 1424
StyI CCWWGG 3 cut(s) 1124, 1799, 2335
TaaI ACNGT 2 cut(s) 2414, 2914
TaiI ACGT 3 cut(s) 1787, 2638, 2823
TatI WGTACW 4 cut(s) 1345, 1586, 2369, 2430
TauI GCSGC 1 cut(s) 2045
Tru1I TTAA 9 cut(s) 938, 1851, 2021, 2085, 2160, 2253, 2406, 2783, 2870
Tru9I TTAA 9 cut(s) 938, 1851, 2021, 2085, 2160, 2253, 2406, 2783, 2870
TscAI CASTG 5 cut(s) 57, 1153, 1241, 1677, 2919
TseFI GTSAC 6 cut(s) 52, 534, 1696, 1924, 2764, 2857
TseI GCWGC 6 cut(s) 23, 692, 1139, 1142, 1634, 1960
Tsp45I GTSAC 6 cut(s) 52, 534, 1696, 1924, 2764, 2857
TspGWI ACGGA 2 cut(s) 735, 2829
TspRI CASTG 5 cut(s) 57, 1153, 1241, 1677, 2919
Tth111I GACNNNGTC 1 cut(s) 2723
VneI GTGCAC 1 cut(s) 2810
VpaK11BI GGWCC 2 cut(s) 1228, 2527
VspI ATTAAT 1 cut(s) 2085
XagI CCTNNNNNAGG 1 cut(s) 2793
XapI RAATTY 3 cut(s) 1115, 1556, 2650
XbaI TCTAGA 1 cut(s) 1744
XceI RCATGY 4 cut(s) 893, 1139, 1289, 2858
XhoI CTCGAG 1 cut(s) 761
XmiI GTMKAC 2 cut(s) 886, 1164
XmnI GAANNNNTTC 2 cut(s) 2484, 2628
XspI CTAG 7 cut(s) 324, 627, 689, 1517, 1745, 1814, 2588
ZraI GACGTC 1 cut(s) 1785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.