Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006279

Physical Location & Seq

Genomic Coordinates Reverse (-)

28239369 .. 28244079

Gene Structure 4711 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g27200.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3246 bp

ATGCGAAAAAGAGAAACAAAAAATAATATTACCCAGACAGGCCTGACCGGACTTATTCCCAAACGACCCCTCCCTCCTTGCTCCCGAAACTGCTCTTCTGCTTCAGGAGCTGGGCCGAGACTTTTGGAGGGTTTTACAGAAGCTGCAAAAGAGATGGAGTTCGAGAAACGAAAGGCGGCGACGCTAGCGTCACTGCGGTCGGAGGAGACAGACAAATCACCGAAGGGCACGGTGGACGCCCCGATCATACCCCTCCTCGACGCCATCAACAGCCACCCCAATTATTTCACCACCAGCTCCTGCTCCGGCCGTATCTCTATCCTCTCCCAGCCCACGCACGTGACAAATCAGCCCAAGACCAAGAAGAAAGCTCTAGGTGGCACGTGGATCTTCATCTCCCACGACCCGGCGGATGCCGACTCTATCCTCGGCCTCCTCTTCCGCCCCGACCCGAATGACCAGAACGACGCACAGGACCAAAACGACCTCGTTTTTAGATTTGAGCCGCTTATTGTGGCGGTGGAGTGCAGGGACGTGGCGTCGGCGCAGTCGTTGGTTTCGAAGGCGATTGCTTGCGGGTTCAGAGAGTCCGGCATTACGAATTCGAACCGGCGCGTGATTATCGCGATTCGGTGCTCAATTCGGATGGAGGTGCCGCTGGGCAGCAGCCGGGAGGTGATGGTGTCGAGAGAGTACGTGAGGTTCCTTGTCGGGGTGGCGAACGAGAAAATGGAGGCCAACAGGAAGAGGACTGAGGCATTTTTCCAGGCTCTGCAGAGTGAGGGTGGAGGGTTTGTGGGTCCCACCGTTGCTAATGGCGAGATTGCGGCAGATGGGCTTTCGGATTCTGGGTCCAAGGAAGTTCCTGGTGGTTGTAGTCTGTCTGTTGTTGAGATGGAAATTGCCGGTGAACCCGAAGAGAATCTTTTCCTCTGGGGTCATTCGTCTTGTGCATTGGAAATCAAAAACCGGAATGGTGTTCTTGTGTTTGGTGGCTTTGGAGGCATGGGAAGACATGGGAGAAGAAGTAGTTCTTGGCTGGTTGATCCATTGTTTGGCACTTTGAGAGCAATTGATGCAGCCAGCACTCCGTCTCCACGGTTGGGTCACACTTCGTCTCTGGTTGGAGATTGTGTGTTTGTCATTGGAGGCAGGGCTGATCCTGAGAAAATTTTGAGTGATGTTTGGGTCCTCAATACGGCAAAGAATGAATGGAAGTTATTGGATTGTTCTGGGGACAAATTTCCTCCCAGGCATCGGCATGCTGCAGCTGTTGTAGGCTCAAAGATATATGTGTTTGGTGGACTTAACAACGATACCATCAGTTCTTCCTTACATGTTCTCGATACCGATAATCTGCAGTGGAAAGAGTTGATTGTCAGTGGCGAGCATCCATGTGCCAGACATTCTCACTCAATGGTGGCATATAGATCTCAACTTTATATTTTTGGAGGATATAGTGGTGAGAAAACACTTGCAGACTTGTATGTTTTTGATATCCAAACGTGTAAATGGAAGAATGAAAAGGCAGCTGGGAGAAGTCCACATGCAAGGTTCTCCCACTCAATGTTTGTCTACAAGAATTATCTTGGGGTTGTTGGTGGTTGTCCAGTTAAACAACGTTGCCAGGAGTTAGCACTACTTGATTTGAAGGTCCGTGTGTGGAGGCATGTGAAACTTGAATCCAATGGTGAAGATTTATTTGTGCGTGGCACAGCCAATGTTGTTGGCGACAATCTTATTATGATTGGTGGTGGGGCATCTTGTTATGCATTTGGGACAAAGTTTAGCAAGCCCATGAAAATCAACTTGCTTCCTCTTATGAGTTTAGATGATAATATTAAGCCAGATGACAGAGAAATGCATGCTCAACATTATGAAATGGTGAATAATGAGAAAGGTAGACAATTCCAGGATCCACGGGCTGAAGGTGTGCAGAGCTTAAGTGAACCTTGTGATCTGAATTTTGAGAGTGACTTGCCCATGGAAAATGGCATAAGTCAGCAGGTAGAATCATATTGGATTCTACAACTTGAAAGAAAATATGCAAAAGTAGGCAAGGACATATTGAAGAAGTTTGGATGGTTAGATCTTGGAAGGAAGGTGTACTCCAGGAAGAGTGGTTCACATATATGTTTTCCTGTGAACGAAAAATTTTCATGCGTGTTTAAAGAAAACATGCATTCTTTGACAGATTTATCTGAAGGACAGAGTAATCACTTTGTTAAACCAGTTATAGGAGAAGAATTCTCAGTTAATGAGGTGAACTGTTCAAAAGCCTTGGACATTCTAAAGGAATGTGGTGCTACTAAGCTGGCAGCTGAAGAAGTTGAAGTCAAGAGAGCTGCCAAATCTCCCGCGAAAGTAATGAATGAAATTGTGGGCTTGCTGATAAAGGAGAAGGGACTGCCTGCAGAACTCTTAGAGGAGCTACCTGCAAGATGGGAGCGACTTGGAGATATAGTTGTCCTTCCGGCGACATCGTTCAAGGATCCAATATGGGACTCACTAGGGATGGAGCTTTGGCATGTTATTTCCAAATCACTTAACGCTCATCGCCTTGCACGCCAAGGCCGAGTTGCGTCAACCGCTACAAGGGACAGTACATTGGAGATTCTTGTAGGAGATAATGGATGGGTTGAGCATCGCGAAAATGGCATTCTCTATTCTTTCGATGCTACTAAATGTATGTTCTCCTGGGGCAACCTATCTGAGAAGCTCCGCATGGCCTCAATGAATTGCAGAAATGAGATTATTGTGGATTTGTTTGCTGGAATTGGATATTTTGTTCTGCCATTTCTTGTCAGGGCCAACGCAAAACTAGTTTATGCTTGTGAATGGAATCCCCATGCTCTTGAGGCACTCCGGCGAAATGTGCAAGCCAATTCAGTTAGTGACCGATGCATTGTTCTTGAAGGAGATAACCGGATAGTGGCACCCAAAGGAGTGGCTGATCGGGTCTGTCTCGGTCTCATTCCAACAAGTGAAGGTAGCTGGGTCACTGCTGTTAGGGCATTAAGGAGCGAGGGCGGGATGCTACACGTGCATGGGAACGTCAAGGACTCAGAGGAGGATTCATGGGCTAAACATGTATCAGAATCAATAAGTGAAATTGCCAAATCTGATGGTCATTGTTGGGAAGTAAGAGTAGAACATGTCGAGAGGGTGAAATGGTACGCCCCACACATCCGTCATCTTGTTGCAGACGTTAGATGTCGACAGAGTATCCGAACATGGCCAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0031590 GO:0031591 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_1 Kelch_3 Kelch_4 Kelch_5 Kelch_6 Met_10 TYW3

Protein Analysis

1082

Amino Acids

119.67

Weight (kDa)

7.34

Isoelectric Point (pI)

41.12

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04670
fragaria_vesca	FvH4_3g02080 FvH4_3g02080 FvH4_3g02080 FvH4_3g02080 FvH4_3g02080 FvH4_3g02080
malus_domestica	MD10G1321300.v1.1
prunus_persica	Prupe.4G021000_v2.0.a1 Prupe.4G021000_v2.0.a1 Prupe.4G021000_v2.0.a1 Prupe.4G021000_v2.0.a1 Prupe.4G021000_v2.0.a1
pyrus_communis	pycom10g27200
rosa_chinensis	RchiOBHm_Chr5g0003171
rosa_laevigata	RLG00000031082
rosa_multiflora	Rmu_sc0012288.1_g000001
rosa_roxburghii	Rroxscaffold_1G00072840
rosa_rugosa	Rorug04G0401000 Rorug04G0401100
rosa_samantha	Rh5AG025900 Rh5BG027100 Rh5CG028700 Rh5DG025500
rosa_wichuraiana	Rw0G010910 Rw5G002390 Rw5G002400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	187
Acc36I	ACCTGC	2 cut(s)	1996, 2443
AccB1I	GGYRCC	2 cut(s)	652, 2935
AccB7I	CCANNNNNTGG	1 cut(s)	1055
AccI	GTMKAC	3 cut(s)	1575, 1903, 3217
AccII	CGCG	4 cut(s)	615, 626, 2360, 2649
AclI	AACGTT	1 cut(s)	1621
AclWI	GGATC	7 cut(s)	395, 1040, 1154, 1910, 1923, 2486, 2499
AcoI	YGGCCR	2 cut(s)	307, 3236
AcsI	RAATTY	6 cut(s)	601, 1170, 1241, 1963, 2153, 2246
AcuI	CTGAAG	4 cut(s)	87, 1947, 2223, 2343
AcvI	CACGTG	3 cut(s)	340, 384, 3043
AcyI	GRCGYC	3 cut(s)	237, 261, 539
AfaI	GTAC	4 cut(s)	695, 2108, 2605, 3176
AfiI	CCNNNNNNNGG	9 cut(s)	406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
AflII	CTTAAG	1 cut(s)	1942
AflIII	ACRYGT	5 cut(s)	1336, 1505, 3040, 3088, 3154
AgsI	TTSAA	8 cut(s)	1651, 1682, 2036, 2071, 2274, 2333, 2488, 2915
AhdI	GACNNNNNGTC	1 cut(s)	538
AhlI	ACTAGT	1 cut(s)	2821
AjiI	CACGTC	1 cut(s)	535
AjnI	CCWGG	7 cut(s)	765, 865, 1250, 1626, 1911, 2111, 2696
AjuI	GAANNNNNNNTTGG	2 cut(s)	1018, 1050
AleI	CACNNNNGTG	1 cut(s)	338
AloI	GAACNNNNNNTCC	4 cut(s)	2107, 2139, 2772, 2804
Alw21I	GWGCWC	1 cut(s)	638
Alw26I	GTCTC	6 cut(s)	112, 200, 1098, 1122, 2969, 2975
AlwI	GGATC	7 cut(s)	395, 1040, 1154, 1910, 1923, 2486, 2499
AlwNI	CAGNNNCTG	4 cut(s)	110, 143, 300, 772
AoxI	GGCC	9 cut(s)	40, 113, 307, 430, 735, 2572, 2727, 2808, 3236
ApeKI	GCWGC	9 cut(s)	143, 663, 666, 1079, 1265, 1268, 1529, 2318, 2345
ApoI	RAATTY	6 cut(s)	601, 1170, 1241, 1963, 2153, 2246
Asp700I	GAANNNNTTC	2 cut(s)	926, 1030
AspLEI	GCGC	2 cut(s)	547, 615
AspS9I	GGNCC	7 cut(s)	113, 475, 800, 852, 1189, 1654, 2808
AsuC2I	CCSGG	2 cut(s)	407, 671
AsuHPI	GGTGA	9 cut(s)	210, 280, 688, 920, 1475, 1703, 1897, 2275, 3178
AsuII	TTCGAA	2 cut(s)	560, 605
AsuNHI	GCTAGC	1 cut(s)	184
AvaII	GGWCC	5 cut(s)	475, 800, 852, 1189, 1654
BaeGI	GKGCMC	1 cut(s)	230
BalI	TGGCCA	1 cut(s)	3238
BamHI	GGATCC	2 cut(s)	1915, 2491
BanI	GGYRCC	2 cut(s)	652, 2935
BbrPI	CACGTG	3 cut(s)	340, 384, 3043
BbsI	GAAGAC	1 cut(s)	1018
Bbv12I	GWGCWC	1 cut(s)	638
BbvI	GCAGC	9 cut(s)	130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
BceAI	ACGGC	2 cut(s)	294, 1215
BciT130I	CCWGG	7 cut(s)	767, 867, 1252, 1628, 1913, 2113, 2698
BciVI	GTATCC	1 cut(s)	3236
BcnI	CCSGG	2 cut(s)	407, 671
BcoDI	GTCTC	6 cut(s)	112, 200, 1098, 1122, 2969, 2975
BcuI	ACTAGT	1 cut(s)	2821
BfaI	CTAG	4 cut(s)	185, 374, 2510, 2822
BfmI	CTRYAG	4 cut(s)	773, 1266, 1358, 2412
BfrI	CTTAAG	1 cut(s)	1942
BfuAI	ACCTGC	2 cut(s)	1996, 2443
BfuI	GTATCC	1 cut(s)	3236
BglII	AGATCT	2 cut(s)	1430, 2089
Bme1390I	CCNGG	9 cut(s)	407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
Bme18I	GGWCC	5 cut(s)	475, 800, 852, 1189, 1654
BmeRI	GACNNNNNGTC	1 cut(s)	538
BmgBI	CACGTC	1 cut(s)	535
BmgT120I	GGNCC	7 cut(s)	113, 475, 800, 852, 1189, 1654, 2808
BmiI	GGNNCC	9 cut(s)	654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
BmrFI	CCNGG	9 cut(s)	407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
BmsI	GCATC	9 cut(s)	403, 1066, 1264, 1399, 1769, 2653, 2665, 2891, 3024
BmtI	GCTAGC	1 cut(s)	188
BpiI	GAAGAC	1 cut(s)	1018
BpmI	CTGGAG	1 cut(s)	2095
Bpu14I	TTCGAA	2 cut(s)	560, 605
BpuEI	CTTGAG	1 cut(s)	2876
BpuMI	CCSGG	2 cut(s)	407, 671
BsaAI	YACGTR	4 cut(s)	340, 384, 697, 3043
BsaBI	GATNNNNATC	1 cut(s)	392
BsaHI	GRCGYC	3 cut(s)	237, 261, 539
BsaI	GGTCTC	1 cut(s)	2975
BsaJI	CCNNGG	9 cut(s)	427, 855, 1097, 1250, 1919, 1983, 2280, 2569, 2697
BsaWI	WCCGGW	3 cut(s)	47, 969, 2925
Bsc4I	CCNNNNNNNGG	9 cut(s)	406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
Bse118I	RCCGGY	2 cut(s)	609, 905
Bse1I	ACTGG	2 cut(s)	1610, 2231
Bse8I	GATNNNNATC	1 cut(s)	392
BseBI	CCWGG	7 cut(s)	767, 867, 1252, 1628, 1913, 2113, 2698
BseDI	CCNNGG	9 cut(s)	427, 855, 1097, 1250, 1919, 1983, 2280, 2569, 2697
BseGI	GGATG	8 cut(s)	418, 651, 1390, 2087, 2520, 2639, 3039, 3186
BseJI	GATNNNNATC	1 cut(s)	392
BseLI	CCNNNNNNNGG	9 cut(s)	406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
BseMII	CTCAG	5 cut(s)	744, 1155, 2265, 2703, 3078
BseNI	ACTGG	2 cut(s)	1610, 2231
BseRI	GAGGAG	5 cut(s)	218, 245, 425, 2441, 3083
BseSI	GKGCMC	1 cut(s)	230
BseX3I	CGGCCG	1 cut(s)	307
BseXI	GCAGC	9 cut(s)	130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
BseYI	CCCAGC	5 cut(s)	110, 327, 658, 1532, 2994
BsgI	GTGCAG	2 cut(s)	547, 1955
Bsh1236I	CGCG	4 cut(s)	615, 626, 2360, 2649
Bsh1285I	CGRYCG	2 cut(s)	200, 310
BshFI	GGCC	9 cut(s)	42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
BshNI	GGYRCC	2 cut(s)	652, 2935
BsiEI	CGRYCG	2 cut(s)	200, 310
BsiHKAI	GWGCWC	1 cut(s)	638
BslFI	GGGAC	7 cut(s)	545, 786, 1250, 1792, 2418, 2516, 2612
BslI	CCNNNNNNNGG	9 cut(s)	406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
BsmAI	GTCTC	6 cut(s)	112, 200, 1098, 1122, 2969, 2975
BsmBI	CGTCTC	2 cut(s)	1098, 1122
BsmFI	GGGAC	7 cut(s)	545, 786, 1250, 1792, 2418, 2516, 2612
BsmI	GAATGC	2 cut(s)	2182, 2658
BsnI	GGCC	9 cut(s)	42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
Bso31I	GGTCTC	1 cut(s)	2975
Bsp119I	TTCGAA	2 cut(s)	560, 605
Bsp1286I	GDGCHC	2 cut(s)	230, 638
Bsp19I	CCATGG	1 cut(s)	1983
Bsp68I	TCGCGA	2 cut(s)	626, 2649
BspANI	GGCC	9 cut(s)	42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
BspCNI	CTCAG	5 cut(s)	745, 1156, 2264, 2704, 3077
BspFNI	CGCG	4 cut(s)	615, 626, 2360, 2649
BspLI	GGNNCC	9 cut(s)	654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
BspMAI	CTGCAG	4 cut(s)	777, 1270, 1362, 2416
BspMI	ACCTGC	2 cut(s)	1996, 2443
BspOI	GCTAGC	1 cut(s)	188
BspPI	GGATC	7 cut(s)	395, 1040, 1154, 1910, 1923, 2486, 2499
BspQI	GCTCTTC	1 cut(s)	100
BspT104I	TTCGAA	2 cut(s)	560, 605
BspT107I	GGYRCC	2 cut(s)	652, 2935
BspTI	CTTAAG	1 cut(s)	1942
BspTNI	GGTCTC	1 cut(s)	2975
BsrFI	RCCGGY	2 cut(s)	609, 905
BsrI	ACTGG	2 cut(s)	1610, 2231
BssAI	RCCGGY	2 cut(s)	609, 905
BssECI	CCNNGG	9 cut(s)	427, 855, 1097, 1250, 1919, 1983, 2280, 2569, 2697
BssNI	GRCGYC	3 cut(s)	237, 261, 539
BssT1I	CCWWGG	4 cut(s)	855, 1983, 2280, 2569
Bst2UI	CCWGG	7 cut(s)	767, 867, 1252, 1628, 1913, 2113, 2698
Bst4CI	ACNGT	5 cut(s)	232, 808, 1101, 2270, 2603
Bst6I	CTCTTC	5 cut(s)	100, 443, 740, 912, 2111
BstACI	GRCGYC	3 cut(s)	237, 261, 539
BstAFI	CTTAAG	1 cut(s)	1942
BstAPI	GCANNNNNTGC	1 cut(s)	1076
BstBAI	YACGTR	4 cut(s)	340, 384, 697, 3043
BstBI	TTCGAA	2 cut(s)	560, 605
BstDEI	CTNAG	7 cut(s)	753, 1164, 2251, 2310, 2422, 2712, 3064
BstDSI	CCRYGG	3 cut(s)	1097, 1919, 1983
BstF5I	GGATG	8 cut(s)	418, 651, 1390, 2087, 2520, 2639, 3039, 3186
BstFNI	CGCG	4 cut(s)	615, 626, 2360, 2649
BstHHI	GCGC	2 cut(s)	547, 615
BstMAI	GTCTC	6 cut(s)	112, 200, 1098, 1122, 2969, 2975
BstMCI	CGRYCG	2 cut(s)	200, 310
BstNI	CCWGG	7 cut(s)	767, 867, 1252, 1628, 1913, 2113, 2698
BstNSI	RCATGY	9 cut(s)	1265, 1340, 1550, 1673, 1868, 2182, 2531, 3092, 3158
BstSCI	CCNGG	9 cut(s)	405, 669, 765, 865, 1250, 1626, 1911, 2111, 2696
BstSFI	CTRYAG	4 cut(s)	773, 1266, 1358, 2412
BstSLI	GKGCMC	1 cut(s)	230
BstUI	CGCG	4 cut(s)	615, 626, 2360, 2649
BstV1I	GCAGC	9 cut(s)	130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
BstV2I	GAAGAC	1 cut(s)	1018
BstX2I	RGATCY	5 cut(s)	387, 1430, 1915, 2089, 2491
BstXI	CCANNNNNNTGG	1 cut(s)	2947
BstYI	RGATCY	5 cut(s)	387, 1430, 1915, 2089, 2491
BstZI	CGGCCG	1 cut(s)	307
BsuI	GTATCC	1 cut(s)	3236
BsuRI	GGCC	9 cut(s)	42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
BtgI	CCRYGG	3 cut(s)	1097, 1919, 1983
BtgZI	GCGATG	2 cut(s)	2540, 2630
BtrI	CACGTC	1 cut(s)	535
BtsCI	GGATG	8 cut(s)	418, 651, 1390, 2087, 2520, 2639, 3039, 3186
BtsI	GCAGTG	3 cut(s)	191, 1367, 3000
BtsIMutI	CAGTG	4 cut(s)	191, 1367, 1387, 3000
BtuMI	TCGCGA	2 cut(s)	626, 2649
BveI	ACCTGC	2 cut(s)	1996, 2443
CaiI	CAGNNNCTG	4 cut(s)	110, 143, 300, 772
CfoI	GCGC	2 cut(s)	547, 615
Cfr10I	RCCGGY	2 cut(s)	609, 905
Cfr13I	GGNCC	7 cut(s)	113, 475, 800, 852, 1189, 1654, 2808
CseI	GACGC	7 cut(s)	177, 190, 245, 269, 476, 528, 2571
Csp6I	GTAC	4 cut(s)	694, 2107, 2604, 3175
CviQI	GTAC	4 cut(s)	694, 2107, 2604, 3175
DdeI	CTNAG	7 cut(s)	753, 1164, 2251, 2310, 2422, 2712, 3064
DraI	TTTAAA	1 cut(s)	2170
DrdI	GACNNNNNNGTC	1 cut(s)	187
DriI	GACNNNNNGTC	1 cut(s)	538
DseDI	GACNNNNNNGTC	1 cut(s)	187
EaeI	YGGCCR	2 cut(s)	307, 3236
EagI	CGGCCG	1 cut(s)	307
Eam1104I	CTCTTC	5 cut(s)	100, 443, 740, 912, 2111
Eam1105I	GACNNNNNGTC	1 cut(s)	538
EarI	CTCTTC	5 cut(s)	100, 443, 740, 912, 2111
EciI	GGCGGA	2 cut(s)	425, 431
EclXI	CGGCCG	1 cut(s)	307
Eco130I	CCWWGG	4 cut(s)	855, 1983, 2280, 2569
Eco147I	AGGCCT	1 cut(s)	42
Eco31I	GGTCTC	1 cut(s)	2975
Eco32I	GATATC	1 cut(s)	1498
Eco47I	GGWCC	5 cut(s)	475, 800, 852, 1189, 1654
Eco52I	CGGCCG	1 cut(s)	307
Eco57I	CTGAAG	4 cut(s)	87, 1947, 2223, 2343
Eco72I	CACGTG	3 cut(s)	340, 384, 3043
EcoO109I	RGGNCCY	2 cut(s)	800, 1189
EcoRI	GAATTC	2 cut(s)	601, 2246
EcoRII	CCWGG	7 cut(s)	765, 865, 1250, 1626, 1911, 2111, 2696
EcoRV	GATATC	1 cut(s)	1498
EcoT14I	CCWWGG	4 cut(s)	855, 1983, 2280, 2569
EcoT22I	ATGCAT	4 cut(s)	1774, 1866, 2184, 2906
ErhI	CCWWGG	4 cut(s)	855, 1983, 2280, 2569
Esp3I	CGTCTC	2 cut(s)	1098, 1122
FalI	AAGNNNNNCTT	6 cut(s)	909, 941, 1018, 1050, 1936, 1968
FaqI	GGGAC	7 cut(s)	545, 786, 1250, 1792, 2418, 2516, 2612
FauI	CCCGC	3 cut(s)	569, 2365, 3023
FblI	GTMKAC	3 cut(s)	1575, 1903, 3217
FokI	GGATG	8 cut(s)	425, 658, 1377, 2094, 2527, 2646, 3046, 3173
FspBI	CTAG	4 cut(s)	185, 374, 2510, 2822
GlaI	GCGC	2 cut(s)	546, 614
GsaI	CCCAGC	5 cut(s)	114, 331, 662, 1536, 2998
GsuI	CTGGAG	1 cut(s)	2095
HaeIII	GGCC	9 cut(s)	42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
HgaI	GACGC	7 cut(s)	177, 190, 245, 269, 476, 528, 2571
HhaI	GCGC	2 cut(s)	547, 615
Hin1I	GRCGYC	3 cut(s)	237, 261, 539
Hin6I	GCGC	2 cut(s)	545, 613
HinP1I	GCGC	2 cut(s)	545, 613
HincII	GTYRAC	2 cut(s)	2586, 3218
HindII	GTYRAC	2 cut(s)	2586, 3218
HphI	GGTGA	9 cut(s)	210, 280, 688, 920, 1475, 1703, 1897, 2275, 3178
Hpy99I	CGWCG	4 cut(s)	184, 263, 470, 544
HpyCH4III	ACNGT	5 cut(s)	232, 808, 1101, 2270, 2603
HpyCH4IV	ACGT	9 cut(s)	339, 383, 534, 696, 1505, 1621, 3042, 3054, 3207
HpyF3I	CTNAG	7 cut(s)	753, 1164, 2251, 2310, 2422, 2712, 3064
HpySE526I	ACGT	9 cut(s)	339, 383, 534, 696, 1505, 1621, 3042, 3054, 3207
Hsp92I	GRCGYC	3 cut(s)	237, 261, 539
HspAI	GCGC	2 cut(s)	545, 613
KflI	GGGWCCC	1 cut(s)	800
LguI	GCTCTTC	1 cut(s)	100
LmnI	GCTCC	9 cut(s)	86, 107, 302, 308, 2428, 2446, 2518, 2724, 3021
Lsp1109I	GCAGC	9 cut(s)	130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
LweI	GCATC	9 cut(s)	403, 1066, 1264, 1399, 1769, 2653, 2665, 2891, 3024
MaeI	CTAG	4 cut(s)	185, 374, 2510, 2822
MaeII	ACGT	9 cut(s)	339, 383, 534, 696, 1505, 1621, 3042, 3054, 3207
MaeIII	GTNAC	6 cut(s)	189, 340, 1106, 1973, 2894, 2998
MfeI	CAATTG	1 cut(s)	1071
MflI	RGATCY	5 cut(s)	387, 1430, 1915, 2089, 2491
MhlI	GDGCHC	2 cut(s)	230, 638
MlsI	TGGCCA	1 cut(s)	3238
MluNI	TGGCCA	1 cut(s)	3238
MlyI	GAGTC	4 cut(s)	413, 596, 2498, 3056
MmeI	TCCRAC	3 cut(s)	180, 1105, 3002
Mox20I	TGGCCA	1 cut(s)	3238
Mph1103I	ATGCAT	4 cut(s)	1774, 1866, 2184, 2906
MroXI	GAANNNNTTC	2 cut(s)	926, 1030
MscI	TGGCCA	1 cut(s)	3238
MseI	TTAA	9 cut(s)	1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
MslI	CAYNNNNRTG	5 cut(s)	338, 1260, 1395, 2131, 3045
Msp20I	TGGCCA	1 cut(s)	3238
MspA1I	CMGCKG	4 cut(s)	658, 1271, 1532, 2321
MspCI	CTTAAG	1 cut(s)	1942
MspR9I	CCNGG	9 cut(s)	407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
MunI	CAATTG	1 cut(s)	1071
Mva1269I	GAATGC	2 cut(s)	2182, 2658
MvaI	CCWGG	7 cut(s)	767, 867, 1252, 1628, 1913, 2113, 2698
MvnI	CGCG	4 cut(s)	615, 626, 2360, 2649
NciI	CCSGG	2 cut(s)	407, 671
NcoI	CCATGG	1 cut(s)	1983
NheI	GCTAGC	1 cut(s)	184
NlaIV	GGNNCC	9 cut(s)	654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
NmeAIII	GCCGAG	3 cut(s)	141, 408, 2600
NmuCI	GTSAC	6 cut(s)	189, 340, 1106, 1973, 2894, 2998
NruI	TCGCGA	2 cut(s)	626, 2649
NsiI	ATGCAT	4 cut(s)	1774, 1866, 2184, 2906
NspI	RCATGY	9 cut(s)	1265, 1340, 1550, 1673, 1868, 2182, 2531, 3092, 3158
NspV	TTCGAA	2 cut(s)	560, 605
OliI	CACNNNNGTG	1 cut(s)	338
PaeI	GCATGC	2 cut(s)	1265, 1868
PceI	AGGCCT	1 cut(s)	42
PciI	ACATGT	3 cut(s)	1336, 3088, 3154
PciSI	GCTCTTC	1 cut(s)	100
PcsI	WCGNNNNNNNCGW	3 cut(s)	548, 557, 2572
PctI	GAATGC	2 cut(s)	2182, 2658
PdmI	GAANNNNTTC	2 cut(s)	926, 1030
PflMI	CCANNNNNTGG	1 cut(s)	1055
PfoI	TCCNGGA	2 cut(s)	1911, 2111
PleI	GAGTC	4 cut(s)	413, 595, 2498, 3056
PmaCI	CACGTG	3 cut(s)	340, 384, 3043
PmlI	CACGTG	3 cut(s)	340, 384, 3043
PpsI	GAGTC	4 cut(s)	413, 595, 2498, 3056
Ppu21I	YACGTR	4 cut(s)	340, 384, 697, 3043
PpuMI	RGGWCCY	2 cut(s)	800, 1189
PscI	ACATGT	3 cut(s)	1336, 3088, 3154
Psp1406I	AACGTT	1 cut(s)	1621
Psp5II	RGGWCCY	2 cut(s)	800, 1189
Psp6I	CCWGG	7 cut(s)	765, 865, 1250, 1626, 1911, 2111, 2696
PspCI	CACGTG	3 cut(s)	340, 384, 3043
PspFI	CCCAGC	5 cut(s)	110, 327, 658, 1532, 2994
PspGI	CCWGG	7 cut(s)	765, 865, 1250, 1626, 1911, 2111, 2696
PspN4I	GGNNCC	9 cut(s)	654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
PspPI	GGNCC	7 cut(s)	113, 475, 800, 852, 1189, 1654, 2808
PspPPI	RGGWCCY	2 cut(s)	800, 1189
PsrI	GAACNNNNNNTAC	2 cut(s)	686, 718
PstI	CTGCAG	4 cut(s)	777, 1270, 1362, 2416
PstNI	CAGNNNCTG	4 cut(s)	110, 143, 300, 772
PsuI	RGATCY	5 cut(s)	387, 1430, 1915, 2089, 2491
PvuII	CAGCTG	3 cut(s)	1271, 1532, 2321
RruI	TCGCGA	2 cut(s)	626, 2649
RsaI	GTAC	4 cut(s)	695, 2108, 2605, 3176
RsaNI	GTAC	4 cut(s)	694, 2107, 2604, 3175
RseI	CAYNNNNRTG	5 cut(s)	338, 1260, 1395, 2131, 3045
SalI	GTCGAC	1 cut(s)	3216
SapI	GCTCTTC	1 cut(s)	100
SaqAI	TTAA	9 cut(s)	1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
Sau96I	GGNCC	7 cut(s)	113, 475, 800, 852, 1189, 1654, 2808
SchI	GAGTC	4 cut(s)	413, 596, 2498, 3056
ScrFI	CCNGG	9 cut(s)	407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
SduI	GDGCHC	2 cut(s)	230, 638
SfaNI	GCATC	9 cut(s)	403, 1066, 1264, 1399, 1769, 2653, 2665, 2891, 3024
SfcI	CTRYAG	4 cut(s)	773, 1266, 1358, 2412
SfuI	TTCGAA	2 cut(s)	560, 605
SinI	GGWCC	5 cut(s)	475, 800, 852, 1189, 1654
SmiMI	CAYNNNNRTG	5 cut(s)	338, 1260, 1395, 2131, 3045
SmlI	CTYRAG	2 cut(s)	1942, 2855
SmoI	CTYRAG	2 cut(s)	1942, 2855
SpeI	ACTAGT	1 cut(s)	2821
SphI	GCATGC	2 cut(s)	1265, 1868
SseBI	AGGCCT	1 cut(s)	42
SspI	AATATT	2 cut(s)	28, 1840
SspMI	CTAG	4 cut(s)	185, 374, 2510, 2822
StuI	AGGCCT	1 cut(s)	42
StyD4I	CCNGG	9 cut(s)	405, 669, 765, 865, 1250, 1626, 1911, 2111, 2696
StyI	CCWWGG	4 cut(s)	855, 1983, 2280, 2569
TaaI	ACNGT	5 cut(s)	232, 808, 1101, 2270, 2603
TaiI	ACGT	9 cut(s)	342, 386, 537, 699, 1508, 1624, 3045, 3057, 3210
TaqI	TCGA	9 cut(s)	162, 258, 560, 605, 686, 1344, 2673, 3159, 3217
TaqII	GACCGA	2 cut(s)	2913, 2957
TatI	WGTACW	2 cut(s)	2106, 2603
TauI	GCSGC	4 cut(s)	179, 508, 658, 830
Tru1I	TTAA	9 cut(s)	1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
Tru9I	TTAA	9 cut(s)	1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
TscAI	CASTG	4 cut(s)	198, 1367, 1387, 3007
TseFI	GTSAC	6 cut(s)	189, 340, 1106, 1973, 2894, 2998
TseI	GCWGC	9 cut(s)	143, 663, 666, 1079, 1265, 1268, 1529, 2318, 2345
Tsp45I	GTSAC	6 cut(s)	189, 340, 1106, 1973, 2894, 2998
TspGWI	ACGGA	3 cut(s)	1080, 1646, 3179
TspRI	CASTG	4 cut(s)	198, 1367, 1387, 3007
Van91I	CCANNNNNTGG	1 cut(s)	1055
Vha464I	CTTAAG	1 cut(s)	1942
VpaK11BI	GGWCC	5 cut(s)	475, 800, 852, 1189, 1654
XapI	RAATTY	6 cut(s)	601, 1170, 1241, 1963, 2153, 2246
XceI	RCATGY	9 cut(s)	1265, 1340, 1550, 1673, 1868, 2182, 2531, 3092, 3158
XmiI	GTMKAC	3 cut(s)	1575, 1903, 3217
XmnI	GAANNNNTTC	2 cut(s)	926, 1030
XspI	CTAG	4 cut(s)	185, 374, 2510, 2822
Zsp2I	ATGCAT	4 cut(s)	1774, 1866, 2184, 2906

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.