FvH4_3g02080

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006279
Physical Location & Seq
Forward (+)
1053256 .. 1058542
5287 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02080.t2

Sequence Viewer

Length: 2223 bp
ATGGGGAGACATGCGAGAAGAAATCAGTCTTTGCTGGTGGATCCATTGTCTGGTGGTTTGAGAGTGATTCGGGTGGGGAGTGGTCCTTCTTCGAGATTAGGACACACGGCTTGTTTGGTTGGAGATCGTGTGTTTGTTATTGGAGGAAGGGCTGACCCTGAGAAAATTTTGAGTGATGTATGGGTCCTTGATATACAGAAGAATGAATGGACGCTCGTGGAATGTTCTGGTGACGTGTTTCCTCCCAGGCATCGACATGCTGCGGCCGTTGTAGGATCAAAGTTATATGTGTTTGGTGGGCTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGATACAGATAATATGCAGTGGACAGAGATACATGTCAGTGAGGAAGGTCCTTGTGGCCGACATTCTCACTCAATGGTAGCATCGGGATCTCAGCTATATATCTTTGGAGGGTATGATGGTGAGCAAGCACTTGGAGACTTGTACAGGTTTGATACCCAAACATCTAAATGTAGATGGAAAAAAGTTAAGTCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCGCACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACATTGCCAAGAGTTGGCAATTCTTGATTTACGACTGTGCATGTGGAGGCATGTAAAACTGGAATCCACTGGTGAAGATTTGTTTATACGTAGCACTGCCAATGTTGTTGGTGATGAACTTGTTATGATTGGTGGCGGGGCATCTTGTTATGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAATTTACTTCCCCTGAAGCTTAGAGATGATAACTTTAAACCAGTTGTTAGAGAAGTGCATACTGACCAAATCGATATCATGAAGAGTGAAAAGAGAAGACAAGAACAGGTTCAGAGTTCAAAAACATTAACCGGAGCTCCTGACCTAAATTTTAAGAGTGAACCCACAGCTGATGGCATAGGTCAACATGCGGATGCATATTGGGTTCTGAAACTTGAAAAGAAGTATGCGAAAATAGGGAAGGACATACTGAAGAAGTTTGGGTGGTTGGATCTTGCAAGGAAGGTTTACTCTGTTGAAGGTGGTTTACATATTTGTTTCCCTGTGAGCGCAAAGTTTTCTGATGTACTGAAAGAAAATCAGCATAATATGGTAAATTTATTTGAAGGACAGAGTGATCACATTCATAAACCAGCTATAGGAGCAAAATGTTTAATTGATGAGCTCACCTGTTCAAAAGCCTTGGACATTTTAAAGGAATGTGGTGCCACTATGCTGGTAGATGAAGTTGTTGAAATTAAAAAAACAGCCAAGTCTCCCTTGAAAATAATGAGTGAAGCCGTGGCGTCTCTGCTAAAGGATAAAGACCTACCAGCAGGATTGTTGGAGGAGCTGCCAACAAGATGGGAGCGACTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCCATTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGTTATTGCCAAATCAGTAAATGCTGCTCGCCTTGCTCGTCAAGGCCGAGTTGCATCAAATGGTACAAGGGACAGTACTTTGGAGATTCTGATAGGAGATAATGGCTGGGTTGATCATCGTGAAAATGGAATTGTCTATTCTTTTGATGCTACTAAGTGCATGTTCTCCTGGGGTAACCTCTCTGAGAAGCTCCGCATGGGCTCTTTGGATTGTAGGGATGAGATTGTGGTGGATTTATTTGCTGGAATTGGATATTTTGTGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTCGAGGCACTGCGGAGAAACGTACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGAACAACTGCACCTCAAGGAGTGGCTGATCGGGTATGCCTTGGTCTCATTCCGTCAAGTGAATTGAGCTGGGCCACCGCTGTTAGGGCATTAAGGGGTGAGGGTGGAATGCTACATGTGCATGGGAATGTGGTGGACTCGGAGGAGAATTTGTGGACTAAACATATATCGGAATCCATAGCTGAAGCTGCCAGATCTCAAGGTCATTTCTGGGAAGTTTCAATAGAACATTTGGAGAGAGTGAAATGGTACGCCCCACACATCCGTCACGTCGTAGTAGATGTAAGATGTAGACAGGCCCAGAAATAA

Protein Analysis

741

Amino Acids

82.33

Weight (kDa)

8.02

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 34 - 238 2.6e-68 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 288 - 452 4.5e-37 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 302 - 462 4.6e-14 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 323 - 403 3.5e-15 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 327 - 434 4.6e-10 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 337 - 404 2.2e-08 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 340 - 575 5e-28 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_2 PF07646 343 - 386 2.1e-07 Kelch motif
Kelch_1 PF01344 343 - 384 6.4e-10 Kelch motif
NANM PF24996 347 - 463 1.1e-06 N-acetylneuraminate epimerase
Beta-prop_Calicin PF13964 353 - 480 1.8e-10 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 390 - 449 9.5e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 393 - 433 9.1e-08 Kelch motif
Kelch_2 PF07646 393 - 433 6.9e-09 Kelch motif
Beta-prop_FBX42 PF13415 394 - 563 2.6e-28 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 397 - 568 1.5e-40 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 426 - 479 9.3e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 444 - 481 1.8e-08 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 456 - 570 5.4e-14 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 771 - 851 2.2e-15 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 856 - 1014 2.2e-32 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1292
AccB7I CCANNNNNTGG 2 cut(s) 50, 634
AccI GTMKAC 1 cut(s) 2206
AciI CCGC 6 cut(s) 263, 756, 998, 1711, 1855, 1992
AclWI GGATC 5 cut(s) 35, 48, 283, 433, 1086
AcoI YGGCCR 2 cut(s) 264, 394
AcsI RAATTY 4 cut(s) 165, 955, 1183, 2062
AcuI CTGAAG 3 cut(s) 842, 1079, 2118
AcyI GRCGYC 1 cut(s) 1373
AfaI GTAC 9 cut(s) 336, 482, 578, 781, 1155, 1582, 1594, 1866, 2165
AfiI CCNNNNNNNGG 5 cut(s) 50, 552, 634, 1226, 1998
AflIII ACRYGT 3 cut(s) 234, 370, 2029
AhlI ACTAGT 1 cut(s) 1810
AjiI CACGTC 2 cut(s) 235, 2185
AjnI CCWGG 2 cut(s) 245, 1685
Alw21I GWGCWC 2 cut(s) 946, 1254
Alw26I GTCTC 4 cut(s) 468, 1347, 1380, 1964
AlwI GGATC 5 cut(s) 35, 48, 283, 433, 1086
AoxI GGCC 7 cut(s) 264, 394, 1514, 1561, 1797, 1986, 2211
ApeKI GCWGC 5 cut(s) 260, 1420, 1541, 1780, 2102
ApoI RAATTY 4 cut(s) 165, 955, 1183, 2062
Asp700I GAANNNNTTC 1 cut(s) 915
AspLEI GCGC 1 cut(s) 1139
AspS9I GGNCC 6 cut(s) 83, 184, 386, 1797, 1986, 2212
AsuHPI GGTGA 6 cut(s) 242, 470, 704, 743, 1246, 2024
AvaII GGWCC 3 cut(s) 83, 184, 386
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 1292
BanII GRGCYC 3 cut(s) 946, 1254, 1721
BarI GAAGNNNNNNTAC 2 cut(s) 1098, 1130
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 910
Bbv12I GWGCWC 2 cut(s) 946, 1254
BbvCI CCTCAGC 1 cut(s) 1503
BbvI GCAGC 5 cut(s) 247, 1407, 1528, 1767, 2089
BccI CCATC 4 cut(s) 449, 507, 974, 1425
BceAI ACGGC 3 cut(s) 123, 251, 1352
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 2 cut(s) 247, 1687
BciVI GTATCC 1 cut(s) 334
BclI TGATCA 2 cut(s) 1204, 1630
BcoDI GTCTC 4 cut(s) 468, 1347, 1380, 1964
BcuI ACTAGT 1 cut(s) 1810
BfaI CTAG 1 cut(s) 1811
BfmI CTRYAG 1 cut(s) 1224
BfuI GTATCC 1 cut(s) 334
BglII AGATCT 1 cut(s) 2108
BisI GCNGC 6 cut(s) 261, 264, 1421, 1542, 1781, 2103
BlsI GCNGC 6 cut(s) 262, 265, 1422, 1543, 1782, 2104
BmcAI AGTACT 1 cut(s) 1594
Bme1390I CCNGG 2 cut(s) 247, 1687
Bme18I GGWCC 3 cut(s) 83, 184, 386
BmgBI CACGTC 2 cut(s) 235, 2185
BmgT120I GGNCC 6 cut(s) 83, 184, 386, 1797, 1986, 2212
BmiI GGNNCC 3 cut(s) 42, 185, 1294
BmrFI CCNGG 2 cut(s) 247, 1687
BmsI GCATC 9 cut(s) 259, 301, 428, 770, 991, 1580, 1654, 1880, 1902
BpiI GAAGAC 1 cut(s) 910
Bpu10I CCTNAGC 1 cut(s) 1503
BpuEI CTTGAG 2 cut(s) 1914, 2097
Bsa29I ATCGAT 2 cut(s) 879, 1888
BsaAI YACGTR 1 cut(s) 710
BsaHI GRCGYC 1 cut(s) 1373
BsaI GGTCTC 1 cut(s) 1964
BsaJI CCNNGG 6 cut(s) 245, 1269, 1368, 1686, 1800, 1954
BsaWI WCCGGW 1 cut(s) 938
BsaXI ACNNNNNCTCC 8 cut(s) 114, 144, 928, 958, 1437, 1467, 2051, 2081
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 552, 634, 1226, 1998
Bse1I ACTGG 6 cut(s) 342, 611, 684, 694, 848, 1463
Bse3DI GCAATG 1 cut(s) 622
BseBI CCWGG 2 cut(s) 247, 1687
BseCI ATCGAT 2 cut(s) 879, 1888
BseDI CCNNGG 6 cut(s) 245, 1269, 1368, 1686, 1800, 1954
BseGI GGATG 3 cut(s) 1006, 1741, 2175
BseLI CCNNNNNNNGG 5 cut(s) 50, 552, 634, 1226, 1998
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 4 cut(s) 150, 443, 1494, 1692
BseNI ACTGG 6 cut(s) 342, 611, 684, 694, 848, 1463
BseRI GAGGAG 3 cut(s) 1430, 1520, 2072
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 5 cut(s) 247, 1407, 1528, 1767, 2089
BseYI CCCAGC 2 cut(s) 1623, 1983
BsgI GTGCAG 1 cut(s) 1908
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 7 cut(s) 266, 396, 1516, 1563, 1799, 1988, 2213
BshNI GGYRCC 1 cut(s) 1292
BshVI ATCGAT 2 cut(s) 879, 1888
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 946, 1254
BsiSI CCGG 1 cut(s) 939
BslFI GGGAC 3 cut(s) 528, 1505, 1601
BslI CCNNNNNNNGG 5 cut(s) 50, 552, 634, 1226, 1998
BsmAI GTCTC 4 cut(s) 468, 1347, 1380, 1964
BsmBI CGTCTC 1 cut(s) 1380
BsmFI GGGAC 3 cut(s) 528, 1505, 1601
BsmI GAATGC 1 cut(s) 2028
BsnI GGCC 7 cut(s) 266, 396, 1516, 1563, 1799, 1988, 2213
Bso31I GGTCTC 1 cut(s) 1964
Bsp1286I GDGCHC 3 cut(s) 946, 1254, 1721
Bsp1407I TGTACA 2 cut(s) 480, 576
BspACI CCGC 6 cut(s) 263, 756, 998, 1711, 1855, 1992
BspANI GGCC 7 cut(s) 266, 396, 1516, 1563, 1799, 1988, 2213
BspCNI CTCAG 4 cut(s) 151, 442, 1495, 1693
BspDI ATCGAT 2 cut(s) 879, 1888
BspHI TCATGA 1 cut(s) 885
BspLI GGNNCC 3 cut(s) 42, 185, 1294
BspPI GGATC 5 cut(s) 35, 48, 283, 433, 1086
BspT107I GGYRCC 1 cut(s) 1292
BspTNI GGTCTC 1 cut(s) 1964
BsrDI GCAATG 1 cut(s) 622
BsrGI TGTACA 2 cut(s) 480, 576
BsrI ACTGG 6 cut(s) 342, 611, 684, 694, 848, 1463
BssECI CCNNGG 6 cut(s) 245, 1269, 1368, 1686, 1800, 1954
BssNI GRCGYC 1 cut(s) 1373
BssSI CACGAG 1 cut(s) 215
BssT1I CCWWGG 3 cut(s) 1269, 1800, 1954
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 2 cut(s) 247, 1687
Bst4CI ACNGT 2 cut(s) 657, 1592
Bst6I CTCTTC 1 cut(s) 884
BstACI GRCGYC 1 cut(s) 1373
BstAUI TGTACA 2 cut(s) 480, 576
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 465, 1546
BstDEI CTNAG 6 cut(s) 159, 429, 827, 1503, 1671, 1701
BstDSI CCRYGG 1 cut(s) 1368
BstEII GGTNACC 1 cut(s) 1691
BstF5I GGATG 3 cut(s) 1006, 1741, 2175
BstHHI GCGC 1 cut(s) 1139
BstMAI GTCTC 4 cut(s) 468, 1347, 1380, 1964
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 6 cut(s) 1229, 1550, 1847, 2000, 2032, 2102
BstNI CCWGG 2 cut(s) 247, 1687
BstNSI RCATGY 9 cut(s) 14, 260, 335, 374, 664, 674, 998, 1681, 2033
BstPI GGTNACC 1 cut(s) 1691
BstSCI CCNGG 2 cut(s) 245, 1685
BstSFI CTRYAG 1 cut(s) 1224
BstSNI TACGTA 1 cut(s) 710
BstV1I GCAGC 5 cut(s) 247, 1407, 1528, 1767, 2089
BstV2I GAAGAC 1 cut(s) 910
BstX2I RGATCY 4 cut(s) 40, 425, 1078, 2108
BstXI CCANNNNNNTGG 2 cut(s) 1303, 1431
BstYI RGATCY 4 cut(s) 40, 425, 1078, 2108
BstZI CGGCCG 1 cut(s) 264
Bsu15I ATCGAT 2 cut(s) 879, 1888
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 7 cut(s) 266, 396, 1516, 1563, 1799, 1988, 2213
BsuTUI ATCGAT 2 cut(s) 879, 1888
BtgI CCRYGG 1 cut(s) 1368
BtrI CACGTC 2 cut(s) 235, 2185
BtsCI GGATG 3 cut(s) 1006, 1741, 2175
BtsI GCAGTG 3 cut(s) 362, 714, 1850
BtsIMutI CAGTG 7 cut(s) 362, 382, 687, 714, 1470, 1850, 1888
Cac8I GCNNGC 2 cut(s) 465, 1546
CciI TCATGA 1 cut(s) 885
CfoI GCGC 1 cut(s) 1139
Cfr13I GGNCC 6 cut(s) 83, 184, 386, 1797, 1986, 2212
ClaI ATCGAT 2 cut(s) 879, 1888
CseI GACGC 2 cut(s) 220, 1362
Csp6I GTAC 9 cut(s) 335, 481, 577, 780, 1154, 1581, 1593, 1865, 2164
CviQI GTAC 9 cut(s) 335, 481, 577, 780, 1154, 1581, 1593, 1865, 2164
DdeI CTNAG 6 cut(s) 159, 429, 827, 1503, 1671, 1701
DraI TTTAAA 2 cut(s) 844, 1281
EaeI YGGCCR 2 cut(s) 264, 394
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 884
EarI CTCTTC 1 cut(s) 884
Ecl136II GAGCTC 2 cut(s) 944, 1252
EclXI CGGCCG 1 cut(s) 264
Eco105I TACGTA 1 cut(s) 710
Eco130I CCWWGG 3 cut(s) 1269, 1800, 1954
Eco24I GRGCYC 3 cut(s) 946, 1254, 1721
Eco31I GGTCTC 1 cut(s) 1964
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 3 cut(s) 83, 184, 386
Eco52I CGGCCG 1 cut(s) 264
Eco53kI GAGCTC 2 cut(s) 944, 1252
Eco57I CTGAAG 3 cut(s) 842, 1079, 2118
Eco91I GGTNACC 1 cut(s) 1691
EcoICRI GAGCTC 2 cut(s) 944, 1252
EcoO109I RGGNCCY 2 cut(s) 184, 386
EcoO65I GGTNACC 1 cut(s) 1691
EcoRII CCWGG 2 cut(s) 245, 1685
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 3 cut(s) 1269, 1800, 1954
EcoT22I ATGCAT 3 cut(s) 775, 1006, 1895
EcoT38I GRGCYC 3 cut(s) 946, 1254, 1721
ErhI CCWWGG 3 cut(s) 1269, 1800, 1954
Esp3I CGTCTC 1 cut(s) 1380
FalI AAGNNNNNCTT 2 cut(s) 1331, 1363
FaqI GGGAC 3 cut(s) 528, 1505, 1601
FauI CCCGC 1 cut(s) 749
FbaI TGATCA 2 cut(s) 1204, 1630
FblI GTMKAC 1 cut(s) 2206
Fnu4HI GCNGC 6 cut(s) 261, 264, 1421, 1542, 1781, 2103
FokI GGATG 3 cut(s) 1013, 1748, 2162
FriOI GRGCYC 3 cut(s) 946, 1254, 1721
Fsp4HI GCNGC 6 cut(s) 261, 264, 1421, 1542, 1781, 2103
FspBI CTAG 1 cut(s) 1811
GlaI GCGC 1 cut(s) 1138
GluI GCNGC 6 cut(s) 261, 264, 1421, 1542, 1781, 2103
GsaI CCCAGC 2 cut(s) 1627, 1987
HaeIII GGCC 7 cut(s) 266, 396, 1516, 1563, 1799, 1988, 2213
HapII CCGG 1 cut(s) 939
HgaI GACGC 2 cut(s) 220, 1362
HhaI GCGC 1 cut(s) 1139
Hin1I GRCGYC 1 cut(s) 1373
Hin6I GCGC 1 cut(s) 1137
HinP1I GCGC 1 cut(s) 1137
HincII GTYRAC 1 cut(s) 992
HindII GTYRAC 1 cut(s) 992
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 8 cut(s) 67, 683, 1480, 1493, 1603, 1831, 2051, 2087
HpaII CCGG 1 cut(s) 939
HphI GGTGA 6 cut(s) 242, 470, 704, 743, 1246, 2024
Hpy166II GTNNAC 9 cut(s) 360, 577, 968, 992, 1096, 1115, 2050, 2070, 2207
Hpy188I TCNGA 7 cut(s) 921, 1017, 1150, 1608, 1702, 2056, 2086
Hpy188III TCNNGA 8 cut(s) 93, 423, 644, 886, 947, 1477, 1637, 1901
Hpy8I GTNNAC 9 cut(s) 360, 577, 968, 992, 1096, 1115, 2050, 2070, 2207
Hpy99I CGWCG 1 cut(s) 2189
HpyAV CCTTC 8 cut(s) 96, 141, 377, 1042, 1084, 1100, 1187, 1898
HpyCH4III ACNGT 2 cut(s) 657, 1592
HpyCH4IV ACGT 4 cut(s) 234, 709, 1863, 2184
HpyF10VI GCNNNNNNNGC 6 cut(s) 1229, 1550, 1847, 2000, 2032, 2102
HpyF3I CTNAG 6 cut(s) 159, 429, 827, 1503, 1671, 1701
HpySE526I ACGT 4 cut(s) 234, 709, 1863, 2184
Hsp92I GRCGYC 1 cut(s) 1373
HspAI GCGC 1 cut(s) 1137
Ksp22I TGATCA 2 cut(s) 1204, 1630
LmnI GCTCC 7 cut(s) 941, 949, 1229, 1417, 1435, 1507, 1713
Lsp1109I GCAGC 5 cut(s) 247, 1407, 1528, 1767, 2089
LweI GCATC 9 cut(s) 259, 301, 428, 770, 991, 1580, 1654, 1880, 1902
MaeI CTAG 1 cut(s) 1811
MaeII ACGT 4 cut(s) 234, 709, 1863, 2184
MaeIII GTNAC 5 cut(s) 230, 316, 1465, 1691, 2180
MboII GAAGA 8 cut(s) 30, 81, 211, 315, 707, 901, 915, 1072
MfeI CAATTG 1 cut(s) 1497
MflI RGATCY 4 cut(s) 40, 425, 1078, 2108
MhlI GDGCHC 3 cut(s) 946, 1254, 1721
MlyI GAGTC 2 cut(s) 1487, 2045
MmeI TCCRAC 3 cut(s) 100, 1056, 1392
Mph1103I ATGCAT 3 cut(s) 775, 1006, 1895
MroXI GAANNNNTTC 1 cut(s) 915
MseI TTAA 9 cut(s) 303, 525, 843, 935, 960, 1241, 1280, 1326, 2006
MslI CAYNNNNRTG 7 cut(s) 255, 375, 505, 572, 999, 2034, 2040
MspA1I CMGCKG 2 cut(s) 977, 1994
MspI CCGG 1 cut(s) 939
MspR9I CCNGG 2 cut(s) 247, 1687
MunI CAATTG 1 cut(s) 1497
Mva1269I GAATGC 1 cut(s) 2028
MvaI CCWGG 2 cut(s) 247, 1687
MwoI GCNNNNNNNGC 6 cut(s) 1229, 1550, 1847, 2000, 2032, 2102
NlaIV GGNNCC 3 cut(s) 42, 185, 1294
NmeAIII GCCGAG 1 cut(s) 1589
NmuCI GTSAC 4 cut(s) 230, 316, 1465, 2180
NsiI ATGCAT 3 cut(s) 775, 1006, 1895
NspI RCATGY 9 cut(s) 14, 260, 335, 374, 664, 674, 998, 1681, 2033
PagI TCATGA 1 cut(s) 885
PciI ACATGT 2 cut(s) 370, 2029
PcsI WCGNNNNNNNCGW 2 cut(s) 1552, 1561
PctI GAATGC 1 cut(s) 2028
PdmI GAANNNNTTC 1 cut(s) 915
PfeI GAWTC 6 cut(s) 67, 683, 1480, 1603, 1831, 2087
PflMI CCANNNNNTGG 2 cut(s) 50, 634
PkrI GCNGC 6 cut(s) 262, 265, 1422, 1543, 1782, 2104
PleI GAGTC 2 cut(s) 1487, 2045
PpsI GAGTC 2 cut(s) 1487, 2045
Ppu21I YACGTR 1 cut(s) 710
PpuMI RGGWCCY 2 cut(s) 184, 386
PscI ACATGT 2 cut(s) 370, 2029
Psp124BI GAGCTC 2 cut(s) 946, 1254
Psp5II RGGWCCY 2 cut(s) 184, 386
Psp6I CCWGG 2 cut(s) 245, 1685
PspEI GGTNACC 1 cut(s) 1691
PspFI CCCAGC 2 cut(s) 1623, 1983
PspGI CCWGG 2 cut(s) 245, 1685
PspN4I GGNNCC 3 cut(s) 42, 185, 1294
PspPI GGNCC 6 cut(s) 83, 184, 386, 1797, 1986, 2212
PspPPI RGGWCCY 2 cut(s) 184, 386
PsuI RGATCY 4 cut(s) 40, 425, 1078, 2108
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 9 cut(s) 336, 482, 578, 781, 1155, 1582, 1594, 1866, 2165
RsaNI GTAC 9 cut(s) 335, 481, 577, 780, 1154, 1581, 1593, 1865, 2164
RseI CAYNNNNRTG 7 cut(s) 255, 375, 505, 572, 999, 2034, 2040
SacI GAGCTC 2 cut(s) 946, 1254
SaqAI TTAA 9 cut(s) 303, 525, 843, 935, 960, 1241, 1280, 1326, 2006
SatI GCNGC 6 cut(s) 261, 264, 1421, 1542, 1781, 2103
Sau96I GGNCC 6 cut(s) 83, 184, 386, 1797, 1986, 2212
ScaI AGTACT 1 cut(s) 1594
SchI GAGTC 2 cut(s) 1487, 2045
ScrFI CCNGG 2 cut(s) 247, 1687
SduI GDGCHC 3 cut(s) 946, 1254, 1721
SfaNI GCATC 9 cut(s) 259, 301, 428, 770, 991, 1580, 1654, 1880, 1902
SfcI CTRYAG 1 cut(s) 1224
SinI GGWCC 3 cut(s) 83, 184, 386
SmiMI CAYNNNNRTG 7 cut(s) 255, 375, 505, 572, 999, 2034, 2040
SmlI CTYRAG 2 cut(s) 1929, 2112
SmoI CTYRAG 2 cut(s) 1929, 2112
SnaBI TACGTA 1 cut(s) 710
SpeI ACTAGT 1 cut(s) 1810
SsiI CCGC 6 cut(s) 263, 756, 998, 1711, 1855, 1992
SspMI CTAG 1 cut(s) 1811
SstI GAGCTC 2 cut(s) 946, 1254
StyD4I CCNGG 2 cut(s) 245, 1685
StyI CCWWGG 3 cut(s) 1269, 1800, 1954
TaaI ACNGT 2 cut(s) 657, 1592
TaiI ACGT 4 cut(s) 237, 712, 1866, 2187
TaqI TCGA 5 cut(s) 92, 253, 879, 1845, 1888
TatI WGTACW 5 cut(s) 334, 480, 576, 1153, 1592
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 6 cut(s) 67, 683, 1480, 1603, 1831, 2087
Tru1I TTAA 9 cut(s) 303, 525, 843, 935, 960, 1241, 1280, 1326, 2006
Tru9I TTAA 9 cut(s) 303, 525, 843, 935, 960, 1241, 1280, 1326, 2006
TscAI CASTG 7 cut(s) 362, 382, 694, 721, 1470, 1857, 1888
TseFI GTSAC 4 cut(s) 230, 316, 1465, 2180
TseI GCWGC 5 cut(s) 260, 1420, 1541, 1780, 2102
Tsp45I GTSAC 4 cut(s) 230, 316, 1465, 2180
TspDTI ATGAA 6 cut(s) 219, 750, 815, 902, 1202, 1326
TspGWI ACGGA 2 cut(s) 1956, 2168
TspRI CASTG 7 cut(s) 362, 382, 694, 721, 1470, 1857, 1888
Van91I CCANNNNNTGG 2 cut(s) 50, 634
VpaK11BI GGWCC 3 cut(s) 83, 184, 386
XapI RAATTY 4 cut(s) 165, 955, 1183, 2062
XceI RCATGY 9 cut(s) 14, 260, 335, 374, 664, 674, 998, 1681, 2033
XmiI GTMKAC 1 cut(s) 2206
XmnI GAANNNNTTC 1 cut(s) 915
XspI CTAG 1 cut(s) 1811
ZrmI AGTACT 1 cut(s) 1594
Zsp2I ATGCAT 3 cut(s) 775, 1006, 1895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.