Rroxscaffold_1G00072840

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006279
Physical Location & Seq
Reverse (-)
93827292 .. 93833717
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072840.1

Sequence Viewer

Length: 3057 bp
ATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGGCGTCGCTGAGATCGGAGGTGACGGACAAGTCGCCGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACGTCATCAACGGCCACCGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGGGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGACCTCGTCTTCTGCTCCTCCGATCAGAATGAGCCGGAGCTCCCAGACGACCAAAACGACGTCGTTTTCAGATTCGAGCCGCTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCTATTCGCTGCTCGATACGATTGGAGGTTCCGCTCGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGAGGCTTTTCTGGAGGCTCTGCAGAGTGGGGAGAGCGGAGAGTTTATGGCTCCGGCTGGCGGAAATTACGAGAATGCCCCTTCCGGGTTGAAGGGAGATAGTGGATGCTCTAGTCTGTCAGTTGTTCCGATAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCGGCTTGCTCATTGGTGAAGAAGGTGGATAATGGGATTCTTGTTTTTGGTGGATTCGGAGGCATTGGAAGACATGCGAGGAGAAATCAGTGTTTGCTGGTTGATACATTGTCTGGTAGTTTGAGAGGGATTAGCGTAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGAGATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCGCCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAGGATCAAAGATATATGTGTTTGGTGGACTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGATACAGATAATCTGCAGTGGAGAAAGATAGTTGTCAGTGAGGAAGGTCCGTGTGCCCGACATTCACACTCAATGGTGGCATCGGGATCTCAGCTATATATGTTTGGAGGATATGATGGTGAGGAAGCAATTGGAGACTTGTACAGGTTTGATACTGAAAAATCTAATTGTAAATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCGCATGCAAGGTTCTCACACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACGTTGCCAAGAGTTGGCAATACTTGATTTACGACTGTGCATGTGGAGGCATGTGAAACTGGAATCCAACGGCGAAGATTTGTTTGTACGTAGCACTGCCAATGTTTTTGGTGATGATCTTGTTATGATTGGTGGTGGGGCATCTTGCTATGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCTCTGATGCTGAGAGATGATAATCGTAAACCACTCGTTGGAGAACTGCATACTGACCAAATTGATATTATGAAGAGCGAAAAGAGTAGACATCCACAGGTTCAAAATGTTAAAACATTAACCGAAGCTCCTGACCTAAATTTTAAGAGTGAATTACCCATGGCTGATGGCATTGGTCAACAGGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGGAAGGACATACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCGTGGAAGGTGGTTTACATATTTGTTTCCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCAGCATCATATGGCAAATTCATTTGAAGGACAGAGTGATCACATTTGTAAGCCTGATATAGGAAAGGAATGTTCGATTGATGAGGTCACCTGTTCAACAGCCTTAGACATTCTAAAGGAATGTGGTGCAACGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAGTGATGCCGTGGTGTCTCTGCTGGAGGATAAAGGCCTACCTGCAGGACTGTTGGAGGAGCTGCCAACCAGATGGGAGCGCCTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCTGTTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGCTGTTGCCAAATCAGTAAATGCTGTTCGCCTTGCTCGCCAAGGACGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGCTGGGTTGATCATCGTGAAAATGGAATTCTCTATTCTTTTGATGCTACTAAGTGCATGTTCTCCTGGGGCAACCTTTCTGAGAAGCTCCGCATGGGCTATCTGGATTGTAGAGATGAGATCATTGTGGATTTATTTGCTGGAATTGGATATTTTGTGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGCGGCACTCCGGAGAAACGTACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGGACAACGGCACCTACAGGAGTGGCTGATCGGGTCTGCCTTGGTCTCATTCCATCAAGTGAATCTAGCTGGGCCACTGCTGTTAGGGCATTAAGGGGTGAGGGTGGGATGCTACATGTGCATGGGAATGTGGTGGACTCAGAGGAGAATTCATGGACCAATCATGTAACAGAATCAATAGCTGAAACTGCTAGATCTCAAGGTCGTTGTTGGGAGGTTTCAATAGAACATTTGGAGAGAGTGAAATGGTACGCCCCACACATCCGCCACGTTGTTGCAGATGTAATATGTAGACAGACCCAGAAATAA

Protein Analysis

1018

Amino Acids

112.26

Weight (kDa)

6.5

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 205 1.2e-66 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 254 - 427 3.5e-41 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 263 - 380 6.2e-19 FBX42, beta-propeller domain
Kelch_HCF PF13854 268 - 428 1.6e-14 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 271 - 350 2.4e-07 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 295 - 399 6.3e-11 FKB95, Kelch-repeats domain
Kelch_HCF PF13854 305 - 529 1.8e-20 Host cell factor, Kelch-repeats domain
Beta-prop_TYW4 PF13418 306 - 541 5.7e-27 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 308 - 348 3e-08 Kelch motif
Kelch_2 PF07646 308 - 349 5.2e-06 Kelch motif
NANM PF24996 313 - 429 2.6e-06 N-acetylneuraminate epimerase
Beta-prop_Calicin PF13964 319 - 451 2.1e-09 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 335 - 407 7.6e-11 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 359 - 399 3.7e-08 Kelch motif
Kelch_1 PF01344 359 - 400 3.5e-09 Kelch motif
Beta-prop_FBX42 PF13415 360 - 530 6.5e-27 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 363 - 534 5.9e-39 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 396 - 445 1.8e-07 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 410 - 446 2.9e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 422 - 536 1.1e-12 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 741 - 818 1.5e-13 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 823 - 962 3.2e-31 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 34, 61
AatII GACGTC 2 cut(s) 111, 327
Acc36I ACCTGC 2 cut(s) 376, 2242
AccB1I GGYRCC 1 cut(s) 2758
AccB7I CCANNNNNTGG 2 cut(s) 1465, 1685
AccBSI CCGCTC 3 cut(s) 182, 496, 627
AccI GTMKAC 2 cut(s) 1735, 3040
AccII CGCG 3 cut(s) 126, 510, 528
AccIII TCCGGA 1 cut(s) 2688
AclI AACGTT 1 cut(s) 1453
AclWI GGATC 4 cut(s) 242, 980, 1114, 1264
AcoI YGGCCR 3 cut(s) 118, 154, 243
AcsI RAATTY 7 cut(s) 566, 996, 1786, 1976, 2017, 2481, 2896
AcyI GRCGYC 4 cut(s) 35, 108, 324, 1361
AfaI GTAC 9 cut(s) 1167, 1313, 1409, 1539, 1612, 2416, 2428, 2700, 2999
AfiI CCNNNNNNNGG 9 cut(s) 186, 216, 650, 675, 929, 1465, 1685, 2060, 2755
AflIII ACRYGT 1 cut(s) 2863
AhlI ACTAGT 1 cut(s) 2644
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 2 cut(s) 1076, 2519
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 5 cut(s) 196, 528, 1299, 2214, 2798
AlwI GGATC 4 cut(s) 242, 980, 1114, 1264
AlwNI CAGNNNCTG 2 cut(s) 147, 391
Ama87I CYCGRG 1 cut(s) 497
Aor13HI TCCGGA 1 cut(s) 2688
AoxI GGCC 9 cut(s) 118, 154, 243, 573, 931, 2227, 2348, 2631, 2820
ApeKI GCWGC 5 cut(s) 471, 1091, 1094, 2254, 2614
ApoI RAATTY 7 cut(s) 566, 996, 1786, 1976, 2017, 2481, 2896
AspLEI GCGC 2 cut(s) 385, 2274
AspS9I GGNCC 7 cut(s) 588, 931, 1015, 1217, 2631, 2820, 2904
AsuC2I CCSGG 5 cut(s) 188, 242, 415, 676, 733
AsuHPI GGTGA 9 cut(s) 64, 127, 796, 912, 1073, 1301, 1574, 2080, 2858
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 4 cut(s) 588, 1015, 1217, 2904
BaeGI GKGCMC 2 cut(s) 77, 1228
BanI GGYRCC 1 cut(s) 2758
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 1932, 1964
BbsI GAAGAC 2 cut(s) 265, 844
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 2337
BbvI GCAGC 5 cut(s) 458, 1078, 1106, 2241, 2601
BccI CCATC 5 cut(s) 173, 1280, 1808, 2259, 2809
BceAI ACGGC 7 cut(s) 133, 341, 954, 1082, 1537, 2186, 2772
BciT130I CCWGG 2 cut(s) 1078, 2521
BciVI GTATCC 1 cut(s) 1165
BclI TGATCA 2 cut(s) 2038, 2464
BcnI CCSGG 5 cut(s) 188, 242, 415, 676, 733
BcoDI GTCTC 5 cut(s) 196, 528, 1299, 2214, 2798
BcuI ACTAGT 1 cut(s) 2644
BfaI CTAG 4 cut(s) 702, 2645, 2814, 2940
BfmI CTRYAG 5 cut(s) 611, 1092, 1184, 2235, 2763
BfoI RGCGCY 1 cut(s) 2275
BfuAI ACCTGC 2 cut(s) 376, 2242
BfuI GTATCC 1 cut(s) 1165
BglII AGATCT 2 cut(s) 1912, 2942
BmcAI AGTACT 1 cut(s) 2428
Bme1390I CCNGG 7 cut(s) 188, 242, 415, 676, 733, 1078, 2521
Bme18I GGWCC 4 cut(s) 588, 1015, 1217, 2904
BmeT110I CYCGRG 1 cut(s) 497
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 7 cut(s) 588, 931, 1015, 1217, 2631, 2820, 2904
BmiI GGNNCC 5 cut(s) 492, 542, 642, 1016, 2760
BmrFI CCNGG 7 cut(s) 188, 242, 415, 676, 733, 1078, 2521
BpiI GAAGAC 2 cut(s) 265, 844
BpmI CTGGAG 2 cut(s) 623, 2237
Bpu10I CCTNAGC 1 cut(s) 2337
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 2931
BpuMI CCSGG 5 cut(s) 188, 242, 415, 676, 733
Bsa29I ATCGAT 1 cut(s) 2722
BsaAI YACGTR 1 cut(s) 1541
BsaBI GATNNNNATC 1 cut(s) 1668
BsaHI GRCGYC 4 cut(s) 35, 108, 324, 1361
BsaI GGTCTC 2 cut(s) 196, 2798
BsaWI WCCGGW 3 cut(s) 579, 2688, 2748
BsaXI ACNNNNNCTCC 6 cut(s) 945, 975, 1759, 1789, 2271, 2301
Bsc4I CCNNNNNNNGG 9 cut(s) 186, 216, 650, 675, 929, 1465, 1685, 2060, 2755
Bse1I ACTGG 5 cut(s) 1173, 1442, 1515, 2297, 2416
Bse8I GATNNNNATC 1 cut(s) 1668
BseAI TCCGGA 1 cut(s) 2688
BseBI CCWGG 2 cut(s) 1078, 2521
BseCI ATCGAT 1 cut(s) 2722
BseGI GGATG 6 cut(s) 165, 701, 1738, 1840, 2862, 3009
BseJI GATNNNNATC 1 cut(s) 1668
BseLI CCNNNNNNNGG 9 cut(s) 186, 216, 650, 675, 929, 1465, 1685, 2060, 2755
BseMII CTCAG 9 cut(s) 32, 188, 981, 992, 1274, 1649, 2328, 2526, 2901
BseNI ACTGG 5 cut(s) 1173, 1442, 1515, 2297, 2416
BseRI GAGGAG 7 cut(s) 92, 271, 758, 862, 2264, 2354, 2906
BseSI GKGCMC 2 cut(s) 77, 1228
BseX3I CGGCCG 2 cut(s) 154, 243
BseXI GCAGC 5 cut(s) 458, 1078, 1106, 2241, 2601
BseYI CCCAGC 2 cut(s) 2457, 2817
BsgI GTGCAG 1 cut(s) 385
Bsh1236I CGCG 3 cut(s) 126, 510, 528
Bsh1285I CGRYCG 2 cut(s) 157, 246
BshFI GGCC 9 cut(s) 120, 156, 245, 575, 933, 2229, 2350, 2633, 2822
BshNI GGYRCC 1 cut(s) 2758
BshVI ATCGAT 1 cut(s) 2722
BsiEI CGRYCG 2 cut(s) 157, 246
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 497
BslFI GGGAC 3 cut(s) 383, 2339, 2435
BslI CCNNNNNNNGG 9 cut(s) 186, 216, 650, 675, 929, 1465, 1685, 2060, 2755
BsmAI GTCTC 5 cut(s) 196, 528, 1299, 2214, 2798
BsmFI GGGAC 3 cut(s) 383, 2339, 2435
BsmI GAATGC 1 cut(s) 670
BsnI GGCC 9 cut(s) 120, 156, 245, 575, 933, 2229, 2350, 2633, 2822
Bso31I GGTCTC 2 cut(s) 196, 2798
BsoBI CYCGRG 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 3 cut(s) 77, 306, 1228
Bsp13I TCCGGA 1 cut(s) 2688
Bsp1407I TGTACA 2 cut(s) 1311, 1407
Bsp19I CCATGG 1 cut(s) 1806
Bsp68I TCGCGA 2 cut(s) 510, 528
BspANI GGCC 9 cut(s) 120, 156, 245, 575, 933, 2229, 2350, 2633, 2822
BspCNI CTCAG 9 cut(s) 33, 187, 982, 993, 1273, 1650, 2329, 2527, 2900
BspDI ATCGAT 1 cut(s) 2722
BspEI TCCGGA 1 cut(s) 2688
BspFNI CGCG 3 cut(s) 126, 510, 528
BspLI GGNNCC 5 cut(s) 492, 542, 642, 1016, 2760
BspMAI CTGCAG 4 cut(s) 615, 1096, 1188, 2239
BspMI ACCTGC 2 cut(s) 376, 2242
BspPI GGATC 4 cut(s) 242, 980, 1114, 1264
BspQI GCTCTTC 1 cut(s) 1715
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 2758
BspTNI GGTCTC 2 cut(s) 196, 2798
BsrBI CCGCTC 3 cut(s) 182, 496, 627
BsrGI TGTACA 2 cut(s) 1311, 1407
BsrI ACTGG 5 cut(s) 1173, 1442, 1515, 2297, 2416
BssNI GRCGYC 4 cut(s) 35, 108, 324, 1361
BssT1I CCWWGG 5 cut(s) 1806, 2275, 2392, 2634, 2788
Bst2UI CCWGG 2 cut(s) 1078, 2521
Bst4CI ACNGT 4 cut(s) 79, 1488, 2244, 2426
Bst6I CTCTTC 2 cut(s) 578, 1715
BstACI GRCGYC 4 cut(s) 35, 108, 324, 1361
BstAUI TGTACA 2 cut(s) 1311, 1407
BstBAI YACGTR 1 cut(s) 1541
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 8 cut(s) 33, 180, 184, 649, 775, 1380, 2352, 2389
BstDSI CCRYGG 4 cut(s) 1098, 1806, 1935, 2202
BstEII GGTNACC 1 cut(s) 2086
BstENI CCTNNNNNAGG 1 cut(s) 2058
BstF5I GGATG 6 cut(s) 165, 701, 1738, 1840, 2862, 3009
BstFNI CGCG 3 cut(s) 126, 510, 528
BstH2I RGCGCY 1 cut(s) 2275
BstHHI GCGC 2 cut(s) 385, 2274
BstMAI GTCTC 5 cut(s) 196, 528, 1299, 2214, 2798
BstMCI CGRYCG 2 cut(s) 157, 246
BstMWI GCNNNNNNNGC 7 cut(s) 32, 37, 2388, 2681, 2834, 2866, 2936
BstNI CCWGG 2 cut(s) 1078, 2521
BstNSI RCATGY 8 cut(s) 845, 1091, 1166, 1382, 1495, 1505, 2515, 2867
BstPI GGTNACC 1 cut(s) 2086
BstSCI CCNGG 7 cut(s) 186, 240, 413, 674, 731, 1076, 2519
BstSFI CTRYAG 5 cut(s) 611, 1092, 1184, 2235, 2763
BstSLI GKGCMC 2 cut(s) 77, 1228
BstSNI TACGTA 1 cut(s) 1541
BstUI CGCG 3 cut(s) 126, 510, 528
BstV1I GCAGC 5 cut(s) 458, 1078, 1106, 2241, 2601
BstV2I GAAGAC 2 cut(s) 265, 844
BstX2I RGATCY 3 cut(s) 1256, 1912, 2942
BstXI CCANNNNNNTGG 1 cut(s) 2265
BstYI RGATCY 3 cut(s) 1256, 1912, 2942
BstZI CGGCCG 2 cut(s) 154, 243
Bsu15I ATCGAT 1 cut(s) 2722
BsuI GTATCC 1 cut(s) 1165
BsuRI GGCC 9 cut(s) 120, 156, 245, 575, 933, 2229, 2350, 2633, 2822
BsuTUI ATCGAT 1 cut(s) 2722
BtgI CCRYGG 4 cut(s) 1098, 1806, 1935, 2202
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 6 cut(s) 165, 701, 1738, 1840, 2862, 3009
BtsI GCAGTG 3 cut(s) 1193, 1545, 2823
BtsIMutI CAGTG 7 cut(s) 863, 1193, 1213, 1545, 2304, 2722, 2823
BtuMI TCGCGA 2 cut(s) 510, 528
BveI ACCTGC 2 cut(s) 376, 2242
Cac8I GCNNGC 8 cut(s) 33, 180, 184, 649, 775, 1380, 2352, 2389
CaiI CAGNNNCTG 2 cut(s) 147, 391
CfoI GCGC 2 cut(s) 385, 2274
Cfr13I GGNCC 7 cut(s) 588, 931, 1015, 1217, 2631, 2820, 2904
ClaI ATCGAT 1 cut(s) 2722
CseI GACGC 4 cut(s) 24, 37, 399, 1350
Csp6I GTAC 9 cut(s) 1166, 1312, 1408, 1538, 1611, 2415, 2427, 2699, 2998
CviQI GTAC 9 cut(s) 1166, 1312, 1408, 1538, 1611, 2415, 2427, 2699, 2998
DrdI GACNNNNNNGTC 2 cut(s) 34, 61
DseDI GACNNNNNNGTC 2 cut(s) 34, 61
EaeI YGGCCR 3 cut(s) 118, 154, 243
EagI CGGCCG 2 cut(s) 154, 243
Eam1104I CTCTTC 2 cut(s) 578, 1715
EarI CTCTTC 2 cut(s) 578, 1715
EciI GGCGGA 2 cut(s) 666, 3002
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 2 cut(s) 154, 243
Eco105I TACGTA 1 cut(s) 1541
Eco130I CCWWGG 5 cut(s) 1806, 2275, 2392, 2634, 2788
Eco147I AGGCCT 1 cut(s) 2229
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 2 cut(s) 196, 2798
Eco47I GGWCC 4 cut(s) 588, 1015, 1217, 2904
Eco52I CGGCCG 2 cut(s) 154, 243
Eco53kI GAGCTC 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 2086
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 2058
EcoO109I RGGNCCY 1 cut(s) 1015
EcoO65I GGTNACC 1 cut(s) 2086
EcoRI GAATTC 2 cut(s) 2481, 2896
EcoRII CCWGG 2 cut(s) 1076, 2519
EcoT14I CCWWGG 5 cut(s) 1806, 2275, 2392, 2634, 2788
EcoT22I ATGCAT 3 cut(s) 1606, 1840, 2729
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 5 cut(s) 1806, 2275, 2392, 2634, 2788
FaqI GGGAC 3 cut(s) 383, 2339, 2435
FauI CCCGC 2 cut(s) 187, 209
FauNDI CATATG 1 cut(s) 2010
FbaI TGATCA 2 cut(s) 2038, 2464
FblI GTMKAC 2 cut(s) 1735, 3040
FokI GGATG 6 cut(s) 152, 708, 1725, 1847, 2869, 2996
FriOI GRGCYC 1 cut(s) 306
FspBI CTAG 4 cut(s) 702, 2645, 2814, 2940
GlaI GCGC 2 cut(s) 384, 2273
GsaI CCCAGC 2 cut(s) 2461, 2821
GsuI CTGGAG 2 cut(s) 623, 2237
HaeII RGCGCY 1 cut(s) 2275
HaeIII GGCC 9 cut(s) 120, 156, 245, 575, 933, 2229, 2350, 2633, 2822
HgaI GACGC 4 cut(s) 24, 37, 399, 1350
HhaI GCGC 2 cut(s) 385, 2274
Hin1I GRCGYC 4 cut(s) 35, 108, 324, 1361
Hin6I GCGC 2 cut(s) 383, 2272
HinP1I GCGC 2 cut(s) 383, 2272
HincII GTYRAC 1 cut(s) 1826
HindII GTYRAC 1 cut(s) 1826
HphI GGTGA 9 cut(s) 64, 127, 796, 912, 1073, 1301, 1574, 2080, 2858
Hpy99I CGWCG 6 cut(s) 31, 40, 110, 326, 329, 362
HpyCH4III ACNGT 4 cut(s) 79, 1488, 2244, 2426
HpyCH4IV ACGT 7 cut(s) 108, 324, 372, 1453, 1540, 2697, 3018
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 37, 2388, 2681, 2834, 2866, 2936
HpySE526I ACGT 7 cut(s) 108, 324, 372, 1453, 1540, 2697, 3018
Hsp92I GRCGYC 4 cut(s) 35, 108, 324, 1361
HspAI GCGC 2 cut(s) 383, 2272
Kpn2I TCCGGA 1 cut(s) 2688
Ksp22I TGATCA 2 cut(s) 2038, 2464
LguI GCTCTTC 1 cut(s) 1715
Lsp1109I GCAGC 5 cut(s) 458, 1078, 1106, 2241, 2601
MaeI CTAG 4 cut(s) 702, 2645, 2814, 2940
MaeII ACGT 7 cut(s) 108, 324, 372, 1453, 1540, 2697, 3018
MaeIII GTNAC 5 cut(s) 52, 1147, 2086, 2299, 2914
MbiI CCGCTC 3 cut(s) 182, 496, 627
MboII GAAGA 9 cut(s) 211, 265, 595, 799, 849, 1042, 1146, 1538, 1732
MfeI CAATTG 2 cut(s) 1299, 2331
MflI RGATCY 3 cut(s) 1256, 1912, 2942
MhlI GDGCHC 3 cut(s) 77, 306, 1228
MlyI GAGTC 4 cut(s) 248, 434, 2321, 2879
MmeI TCCRAC 4 cut(s) 931, 1542, 1666, 2226
Mph1103I ATGCAT 3 cut(s) 1606, 1840, 2729
MroI TCCGGA 1 cut(s) 2688
MseI TTAA 5 cut(s) 1134, 1758, 1766, 1791, 2840
MslI CAYNNNNRTG 4 cut(s) 1086, 1403, 2868, 2874
MspA1I CMGCKG 1 cut(s) 346
MspR9I CCNGG 7 cut(s) 188, 242, 415, 676, 733, 1078, 2521
MunI CAATTG 2 cut(s) 1299, 2331
Mva1269I GAATGC 1 cut(s) 670
MvaI CCWGG 2 cut(s) 1078, 2521
MvnI CGCG 3 cut(s) 126, 510, 528
MwoI GCNNNNNNNGC 7 cut(s) 32, 37, 2388, 2681, 2834, 2866, 2936
NciI CCSGG 5 cut(s) 188, 242, 415, 676, 733
NcoI CCATGG 1 cut(s) 1806
NdeI CATATG 1 cut(s) 2010
NlaIV GGNNCC 5 cut(s) 492, 542, 642, 1016, 2760
NmuCI GTSAC 4 cut(s) 52, 1147, 2086, 2299
NruI TCGCGA 2 cut(s) 510, 528
NsiI ATGCAT 3 cut(s) 1606, 1840, 2729
NspI RCATGY 8 cut(s) 845, 1091, 1166, 1382, 1495, 1505, 2515, 2867
NspV TTCGAA 1 cut(s) 443
PaeI GCATGC 1 cut(s) 1382
PceI AGGCCT 1 cut(s) 2229
PciI ACATGT 1 cut(s) 2863
PciSI GCTCTTC 1 cut(s) 1715
PcsI WCGNNNNNNNCGW 5 cut(s) 123, 243, 318, 567, 2395
PctI GAATGC 1 cut(s) 670
PflFI GACNNNGTC 2 cut(s) 257, 269
PflMI CCANNNNNTGG 2 cut(s) 1465, 1685
PleI GAGTC 4 cut(s) 248, 433, 2321, 2879
PpsI GAGTC 4 cut(s) 248, 433, 2321, 2879
Ppu21I YACGTR 1 cut(s) 1541
PpuMI RGGWCCY 1 cut(s) 1015
PscI ACATGT 1 cut(s) 2863
Psp124BI GAGCTC 1 cut(s) 306
Psp1406I AACGTT 1 cut(s) 1453
Psp5II RGGWCCY 1 cut(s) 1015
Psp6I CCWGG 2 cut(s) 1076, 2519
PspEI GGTNACC 1 cut(s) 2086
PspFI CCCAGC 2 cut(s) 2457, 2817
PspGI CCWGG 2 cut(s) 1076, 2519
PspN4I GGNNCC 5 cut(s) 492, 542, 642, 1016, 2760
PspPI GGNCC 7 cut(s) 588, 931, 1015, 1217, 2631, 2820, 2904
PspPPI RGGWCCY 1 cut(s) 1015
PsrI GAACNNNNNNTAC 4 cut(s) 524, 556, 2361, 2393
PstI CTGCAG 4 cut(s) 615, 1096, 1188, 2239
PstNI CAGNNNCTG 2 cut(s) 147, 391
PsuI RGATCY 3 cut(s) 1256, 1912, 2942
PsyI GACNNNGTC 2 cut(s) 257, 269
RruI TCGCGA 2 cut(s) 510, 528
RsaI GTAC 9 cut(s) 1167, 1313, 1409, 1539, 1612, 2416, 2428, 2700, 2999
RsaNI GTAC 9 cut(s) 1166, 1312, 1408, 1538, 1611, 2415, 2427, 2699, 2998
RseI CAYNNNNRTG 4 cut(s) 1086, 1403, 2868, 2874
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 1715
SaqAI TTAA 5 cut(s) 1134, 1758, 1766, 1791, 2840
Sau96I GGNCC 7 cut(s) 588, 931, 1015, 1217, 2631, 2820, 2904
SbfI CCTGCAGG 1 cut(s) 2239
ScaI AGTACT 1 cut(s) 2428
SchI GAGTC 4 cut(s) 248, 434, 2321, 2879
ScrFI CCNGG 7 cut(s) 188, 242, 415, 676, 733, 1078, 2521
SdaI CCTGCAGG 1 cut(s) 2239
SduI GDGCHC 3 cut(s) 77, 306, 1228
SfcI CTRYAG 5 cut(s) 611, 1092, 1184, 2235, 2763
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 4 cut(s) 588, 1015, 1217, 2904
SmiMI CAYNNNNRTG 4 cut(s) 1086, 1403, 2868, 2874
SmlI CTYRAG 1 cut(s) 2946
SmoI CTYRAG 1 cut(s) 2946
SnaBI TACGTA 1 cut(s) 1541
SpeI ACTAGT 1 cut(s) 2644
SphI GCATGC 1 cut(s) 1382
Sse8387I CCTGCAGG 1 cut(s) 2239
SseBI AGGCCT 1 cut(s) 2229
SspMI CTAG 4 cut(s) 702, 2645, 2814, 2940
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 2229
StyD4I CCNGG 7 cut(s) 186, 240, 413, 674, 731, 1076, 2519
StyI CCWWGG 5 cut(s) 1806, 2275, 2392, 2634, 2788
TaaI ACNGT 4 cut(s) 79, 1488, 2244, 2426
TaiI ACGT 7 cut(s) 111, 327, 375, 1456, 1543, 2700, 3021
TaqII GACCGA 1 cut(s) 605
TatI WGTACW 4 cut(s) 1165, 1311, 1407, 2426
TauI GCSGC 6 cut(s) 26, 216, 346, 380, 383, 2684
Tru1I TTAA 5 cut(s) 1134, 1758, 1766, 1791, 2840
Tru9I TTAA 5 cut(s) 1134, 1758, 1766, 1791, 2840
TscAI CASTG 7 cut(s) 863, 1193, 1213, 1552, 2304, 2722, 2830
TseFI GTSAC 4 cut(s) 52, 1147, 2086, 2299
TseI GCWGC 5 cut(s) 471, 1091, 1094, 2254, 2614
Tsp45I GTSAC 4 cut(s) 52, 1147, 2086, 2299
TspDTI ATGAA 4 cut(s) 1646, 1733, 2010, 2889
TspGWI ACGGA 4 cut(s) 71, 247, 1209, 1924
TspRI CASTG 7 cut(s) 863, 1193, 1213, 1552, 2304, 2722, 2830
Tth111I GACNNNGTC 2 cut(s) 257, 269
Van91I CCANNNNNTGG 2 cut(s) 1465, 1685
VpaK11BI GGWCC 4 cut(s) 588, 1015, 1217, 2904
XagI CCTNNNNNAGG 1 cut(s) 2058
XapI RAATTY 7 cut(s) 566, 996, 1786, 1976, 2017, 2481, 2896
XceI RCATGY 8 cut(s) 845, 1091, 1166, 1382, 1495, 1505, 2515, 2867
XmiI GTMKAC 2 cut(s) 1735, 3040
XspI CTAG 4 cut(s) 702, 2645, 2814, 2940
ZraI GACGTC 2 cut(s) 109, 325
ZrmI AGTACT 1 cut(s) 2428
Zsp2I ATGCAT 3 cut(s) 1606, 1840, 2729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.