Rh5DG025500

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006279
Physical Location & Seq
Forward (+)
1773415 .. 1782796
9382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG025500.1

Sequence Viewer

Length: 3732 bp
ATGCTTAACCATGATTATCTGATCGAGCACATATACGGCAAAGCTTTCCGTCTGGTCAATTTTTATAACACCACAAGCAATATAGATGATGGAAGTGACATCACACTCTGTCCTCCCCACCATCAGCTTTCACTCAACCAGGCCATGTATTTTGTCCCCGTCTCTTCTTTAGTGTACGAGCAAACTGGTATTATCTGTCCGTATACTATCACTCTCCGGCTCAACCGTCACTGGCCGGCCGTGGAGAGGATGCTCACTACTCGGTGGCTGGGCACTGAAATAACGATTGAGCTGGTGCAGAAGGTTTTTACAAGGTCAGGATTTCGAATCGAGCACATGACTTCAATCGAGCCCGGTCTGGGTTATGTTTTGTTTCAAAGTGATTTTGCTATTGCAAATCTAATGTTTGGGAAAAGCAAGATGGAGTATACTCTGGGGGATGAGAACGACAGCTCAATTCAACTGGGAATCCCGGAGAATCTCTTTCTGACTTTCGATTCAGCTAATCCTTACCCCGTCACAGTCAACGATGTGAAAGCCCACTTCAAAAGAGAGAAGTTCAATGTTCTGGGCGTGCAATTTCGCAGGGCTGAAGAGGCAGTGCCACGCTCTTGCTATGTCAGCCTAGAGAGAAGCCCAGAAACCACACACACACAAGGAGACCCAAAAATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCGCTGAGATCGGAGGTGACGGACAAGTCGCCGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACGTCATCAACGGCCACTGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGCGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGACCTCGTCTTCTGCTCCTCCGATCAGAATGAGCAGGAGCTCCAAGACGACCAAAACGACGTCGTTTTCAGATTCGAGCCGCTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCGCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGAGGCTTTTCTGGAGGCTCTGCAGAGTGGGGAGAACGGAGGAGGGTTTGCGCTGGCTCCGGCTGGTGGAAATTACGAGAATGCCCCTTCCGGGTTGAAGGGAGATAATGGATGCTCTAGTCTGTCTGTTGTTCCGATAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCGGCTTGCGCGTTGGAGAAGAAGGTGGATAATGGGATTCTTGTGTTTGGTGGATTCGGAGGCATTGGAAGACATGCGAGGAGAAATCAGTGTTTGCTGGTTGATGCATTGTCTGGTAGTTTGAGAGGGATTAGCATAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGAGATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCGCCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAGGATCAAAGATATATGTGTTTGGTGGACTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGATACAGATAATCTGCAGTGGAGAGAGATAGTTGTCAGTGGGGAAGGTCCATGTGCTCGACATTCACACTCAATGGTGGCATCGGGATCTCAGCTATATATGTTTGGAGGATATGATGGTGAGGAAGCAATTGGAGACTTGTACAGGTTTGATACTGAAAAATCTAAGTGTAAATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCGCACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACATTGCCAAGAGTTGGCAATACTTGATTTACGACTGTGCATGTGGAGGCATGTGAAACTGGAATCCAACGGCGAAGATTTGTTTGTACGTAGCACTGCCAATGTTTTTGGTGATGATCTTGTTATGATTGGTGGCGGGGCATCTTGCTATGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCTCTGATGCTGAGAGATGATAATCGTAAACCACTCGTTGGAGAACTGCATACTGACCAAATTGATATTGTGAAGAGCGAAAAGAGTAGACGTCCACAGGTTCAAAATGTTAAAACATTAACCGAAGCTCCTGACCTAAATTTTAAGAGTGAATTACCCATGGCTGATGGCATTGGTCAACAGGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGGAAGGACATACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCGTGGAAGGTGGTTTACATATTTGTTTTCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCAGCATCATATGGCAAATTCATTTGAAGGACAGAGTGATCACATTTGTAAGCCAGATATAGGAAAGGAATGTTCGATTGATGAGGTCACCTGTTCAACAGCCTTAGACATTCTAAAGGAATGTGGTGCAACGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAGTGATGCCGTGGGGTCTCTGCTGGAGGATAAAGGCCTACCTGCAGGACTGTTGGAGGAGCTGCCAACCAGATGGGAGCGACTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCCGTTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGCTGTTGCCAAATCAGTAAATGCTGTTCGCCTTGCTCGCCAAGGCCGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGCTGGGTTGATCATCGTGAAAATGGAATTCTCTATTCTTTTGATGCTACTAAGTGCATGTTCTCCTGGGGCAACCTTTCTGAGAAGCTCCGCATGGGCTCTCTGGATTGTAGAGATGAGATTATTGTGGATTTATTTGCTGGAATTGGATATTTTGTGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGCGGCACTCCGGAGAAACGTACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGGACAACGGCACCTACAGGAGTGGCTGATCGGGTCTGCCTTGGTCTCATTCCATCAAGTGAATCTAGCTGGGCCACTGCTGTTAGGGCATTAAGGGGTGAGGGTGGGATGCTACATGTGCATGGGAATGTGGTGGACTCACAGGAGAATTCATGGACCAAGCACGTAACAGAATCAATAGCTGAAACTGCTAGATCTCAAGGTCATTGTTGGGAGGTTTCAATAGAACATTTGGAGAGAGTGAAATGGTACGCCCCACACATCCGCCACATTGTTGCAGATGTAAGATGCAGACAGACCCAGAAATAA

Protein Analysis

1243

Amino Acids

137.78

Weight (kDa)

6.41

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 228 - 428 6.1e-67 Methyltransferase TYW3
Beta-prop_FBX42 PF13415 478 - 572 1.7e-11 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 479 - 652 6.6e-41 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 493 - 653 4.5e-15 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 496 - 575 5.4e-07 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 523 - 623 1.6e-10 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 527 - 604 1.7e-17 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 531 - 766 8.2e-27 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_2 PF07646 533 - 574 6.5e-06 Kelch motif
Kelch_1 PF01344 533 - 573 3.8e-08 Kelch motif
Beta-prop_Calicin PF13964 544 - 672 2.5e-09 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 560 - 630 3e-10 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 584 - 625 5.4e-09 Kelch motif
Kelch_2 PF07646 584 - 624 4.1e-08 Kelch motif
Beta-prop_FBX42 PF13415 585 - 755 3.9e-27 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 588 - 758 5.3e-39 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 620 - 670 1.7e-07 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 620 - 706 1.3e-07 Host cell factor, Kelch-repeats domain
Kelch_2 PF07646 635 - 671 3e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 661 - 761 1e-09 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 966 - 1043 2e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 1048 - 1187 1.5e-31 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AasI GACNNNNNNGTC 2 cut(s) 703, 730
AatII GACGTC 4 cut(s) 707, 780, 996, 2416
Acc36I ACCTGC 2 cut(s) 1045, 2917
AccB1I GGYRCC 1 cut(s) 3433
AccB7I CCANNNNNTGG 2 cut(s) 2140, 2360
AccBSI CCGCTC 1 cut(s) 851
AccI GTMKAC 3 cut(s) 203, 428, 2410
AccII CGCG 5 cut(s) 887, 1133, 1179, 1197, 1454
AccIII TCCGGA 1 cut(s) 3363
AclWI GGATC 4 cut(s) 911, 1655, 1789, 1939
AcoI YGGCCR 5 cut(s) 233, 237, 787, 823, 912
AcsI RAATTY 7 cut(s) 1235, 1671, 2461, 2651, 2692, 3156, 3571
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 5 cut(s) 704, 777, 993, 2036, 2413
AflIII ACRYGT 1 cut(s) 3538
AhlI ACTAGT 1 cut(s) 3319
AjiI CACGTC 1 cut(s) 1042
AjnI CCWGG 3 cut(s) 138, 1751, 3194
Alw21I GWGCWC 4 cut(s) 30, 336, 975, 1903
Alw26I GTCTC 7 cut(s) 166, 654, 865, 1197, 1974, 2889, 3473
AlwI GGATC 4 cut(s) 911, 1655, 1789, 1939
AlwNI CAGNNNCTG 2 cut(s) 816, 1060
Aor13HI TCCGGA 1 cut(s) 3363
ApeKI GCWGC 5 cut(s) 1140, 1766, 1769, 2929, 3289
ApoI RAATTY 7 cut(s) 1235, 1671, 2461, 2651, 2692, 3156, 3571
AspLEI GCGC 4 cut(s) 887, 1054, 1312, 1454
AspS9I GGNCC 7 cut(s) 1257, 1606, 1690, 1892, 3306, 3495, 3579
AsuC2I CCSGG 7 cut(s) 354, 473, 857, 911, 1084, 1351, 1408
AsuHPI GGTGA 8 cut(s) 733, 796, 1587, 1748, 1976, 2249, 2755, 3533
AsuII TTCGAA 2 cut(s) 325, 1112
AvaII GGWCC 4 cut(s) 1257, 1690, 1892, 3579
BaeGI GKGCMC 2 cut(s) 275, 746
BanI GGYRCC 1 cut(s) 3433
BanII GRGCYC 3 cut(s) 354, 975, 3230
BarI GAAGNNNNNNTAC 2 cut(s) 2607, 2639
BbsI GAAGAC 2 cut(s) 934, 1519
Bbv12I GWGCWC 4 cut(s) 30, 336, 975, 1903
BbvCI CCTCAGC 1 cut(s) 3012
BbvI GCAGC 5 cut(s) 1127, 1753, 1781, 2916, 3276
BccI CCATC 8 cut(s) 83, 129, 415, 842, 1955, 2483, 2934, 3484
BceAI ACGGC 9 cut(s) 52, 224, 802, 1010, 1629, 1757, 2212, 2861, 3447
BcgI CGANNNNNNTGC 2 cut(s) 2046, 2080
BciT130I CCWGG 3 cut(s) 140, 1753, 3196
BciVI GTATCC 1 cut(s) 1840
BclI TGATCA 2 cut(s) 2713, 3139
BcnI CCSGG 7 cut(s) 354, 473, 857, 911, 1084, 1351, 1408
BcoDI GTCTC 7 cut(s) 166, 654, 865, 1197, 1974, 2889, 3473
BcuI ACTAGT 1 cut(s) 3319
BfaI CTAG 5 cut(s) 626, 1377, 3320, 3489, 3615
BfmI CTRYAG 5 cut(s) 1280, 1767, 1859, 2910, 3438
BfuAI ACCTGC 2 cut(s) 1045, 2917
BfuI GTATCC 1 cut(s) 1840
BglII AGATCT 2 cut(s) 2587, 3617
BmcAI AGTACT 1 cut(s) 3103
Bme18I GGWCC 4 cut(s) 1257, 1690, 1892, 3579
BmgBI CACGTC 1 cut(s) 1042
BmgT120I GGNCC 7 cut(s) 1257, 1606, 1690, 1892, 3306, 3495, 3579
BmiI GGNNCC 5 cut(s) 1161, 1211, 1317, 1691, 3435
BmrI ACTGGG 1 cut(s) 473
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 934, 1519
BpmI CTGGAG 2 cut(s) 1292, 2912
Bpu10I CCTNAGC 1 cut(s) 3012
Bpu14I TTCGAA 2 cut(s) 325, 1112
BpuEI CTTGAG 1 cut(s) 3606
BpuMI CCSGG 7 cut(s) 354, 473, 857, 911, 1084, 1351, 1408
Bsa29I ATCGAT 1 cut(s) 3397
BsaAI YACGTR 2 cut(s) 2216, 3589
BsaBI GATNNNNATC 1 cut(s) 2343
BsaHI GRCGYC 5 cut(s) 704, 777, 993, 2036, 2413
BsaI GGTCTC 4 cut(s) 654, 865, 2889, 3473
BsaWI WCCGGW 3 cut(s) 1248, 3363, 3423
BsaXI ACNNNNNCTCC 6 cut(s) 1620, 1650, 2434, 2464, 2946, 2976
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 8 cut(s) 190, 236, 468, 1848, 2117, 2190, 2972, 3091
Bse3DI GCAATG 1 cut(s) 2128
Bse8I GATNNNNATC 1 cut(s) 2343
BseAI TCCGGA 1 cut(s) 3363
BseBI CCWGG 3 cut(s) 140, 1753, 3196
BseCI ATCGAT 1 cut(s) 3397
BseGI GGATG 7 cut(s) 255, 445, 834, 1376, 2515, 3537, 3684
BseJI GATNNNNATC 1 cut(s) 2343
BseMI GCAATG 1 cut(s) 2128
BseMII CTCAG 8 cut(s) 701, 857, 1656, 1667, 1949, 2324, 3003, 3201
BseNI ACTGG 8 cut(s) 190, 236, 468, 1848, 2117, 2190, 2972, 3091
BseRI GAGGAG 7 cut(s) 761, 940, 1314, 1433, 1537, 2939, 3029
BseSI GKGCMC 2 cut(s) 275, 746
BseX3I CGGCCG 3 cut(s) 237, 823, 912
BseXI GCAGC 5 cut(s) 1127, 1753, 1781, 2916, 3276
BseYI CCCAGC 4 cut(s) 268, 1165, 3132, 3492
BsgI GTGCAG 2 cut(s) 317, 1054
Bsh1236I CGCG 5 cut(s) 887, 1133, 1179, 1197, 1454
Bsh1285I CGRYCG 3 cut(s) 240, 826, 915
BshNI GGYRCC 1 cut(s) 3433
BshVI ATCGAT 1 cut(s) 3397
BsiEI CGRYCG 3 cut(s) 240, 826, 915
BsiHKAI GWGCWC 4 cut(s) 30, 336, 975, 1903
BslFI GGGAC 5 cut(s) 140, 1052, 2034, 3014, 3110
BsmAI GTCTC 7 cut(s) 166, 654, 865, 1197, 1974, 2889, 3473
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 5 cut(s) 140, 1052, 2034, 3014, 3110
BsmI GAATGC 1 cut(s) 1345
Bso31I GGTCTC 4 cut(s) 654, 865, 2889, 3473
Bsp119I TTCGAA 2 cut(s) 325, 1112
Bsp1286I GDGCHC 8 cut(s) 30, 275, 336, 354, 746, 975, 1903, 3230
Bsp13I TCCGGA 1 cut(s) 3363
Bsp1407I TGTACA 2 cut(s) 1986, 2082
Bsp19I CCATGG 1 cut(s) 2481
Bsp68I TCGCGA 3 cut(s) 1133, 1179, 1197
BspCNI CTCAG 8 cut(s) 702, 856, 1657, 1668, 1948, 2325, 3004, 3202
BspDI ATCGAT 1 cut(s) 3397
BspEI TCCGGA 1 cut(s) 3363
BspFNI CGCG 5 cut(s) 887, 1133, 1179, 1197, 1454
BspLI GGNNCC 5 cut(s) 1161, 1211, 1317, 1691, 3435
BspMAI CTGCAG 4 cut(s) 1284, 1771, 1863, 2914
BspMI ACCTGC 2 cut(s) 1045, 2917
BspPI GGATC 4 cut(s) 911, 1655, 1789, 1939
BspQI GCTCTTC 1 cut(s) 2390
BspT104I TTCGAA 2 cut(s) 325, 1112
BspT107I GGYRCC 1 cut(s) 3433
BspTNI GGTCTC 4 cut(s) 654, 865, 2889, 3473
BsrBI CCGCTC 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 2128
BsrFI RCCGGY 1 cut(s) 235
BsrGI TGTACA 2 cut(s) 1986, 2082
BsrI ACTGG 8 cut(s) 190, 236, 468, 1848, 2117, 2190, 2972, 3091
BssAI RCCGGY 1 cut(s) 235
BssNAI GTATAC 2 cut(s) 204, 429
BssNI GRCGYC 5 cut(s) 704, 777, 993, 2036, 2413
BssT1I CCWWGG 4 cut(s) 2481, 3067, 3309, 3463
Bst1107I GTATAC 2 cut(s) 204, 429
Bst2UI CCWGG 3 cut(s) 140, 1753, 3196
Bst4CI ACNGT 6 cut(s) 227, 523, 748, 2163, 2919, 3101
Bst6I CTCTTC 4 cut(s) 169, 588, 1247, 2390
BstACI GRCGYC 5 cut(s) 704, 777, 993, 2036, 2413
BstAUI TGTACA 2 cut(s) 1986, 2082
BstBAI YACGTR 2 cut(s) 2216, 3589
BstBI TTCGAA 2 cut(s) 325, 1112
BstC8I GCNNGC 8 cut(s) 237, 575, 849, 853, 1314, 1450, 3027, 3064
BstDSI CCRYGG 5 cut(s) 240, 1773, 2481, 2610, 2877
BstEII GGTNACC 1 cut(s) 2761
BstF5I GGATG 7 cut(s) 255, 445, 834, 1376, 2515, 3537, 3684
BstFNI CGCG 5 cut(s) 887, 1133, 1179, 1197, 1454
BstHHI GCGC 4 cut(s) 887, 1054, 1312, 1454
BstMAI GTCTC 7 cut(s) 166, 654, 865, 1197, 1974, 2889, 3473
BstMCI CGRYCG 3 cut(s) 240, 826, 915
BstMWI GCNNNNNNNGC 8 cut(s) 596, 621, 706, 3063, 3356, 3509, 3541, 3611
BstNI CCWGG 3 cut(s) 140, 1753, 3196
BstNSI RCATGY 7 cut(s) 1520, 1766, 1841, 2170, 2180, 3190, 3542
BstPI GGTNACC 1 cut(s) 2761
BstSFI CTRYAG 5 cut(s) 1280, 1767, 1859, 2910, 3438
BstSLI GKGCMC 2 cut(s) 275, 746
BstSNI TACGTA 1 cut(s) 2216
BstUI CGCG 5 cut(s) 887, 1133, 1179, 1197, 1454
BstV1I GCAGC 5 cut(s) 1127, 1753, 1781, 2916, 3276
BstV2I GAAGAC 2 cut(s) 934, 1519
BstX2I RGATCY 3 cut(s) 1931, 2587, 3617
BstXI CCANNNNNNTGG 1 cut(s) 2940
BstYI RGATCY 3 cut(s) 1931, 2587, 3617
BstZ17I GTATAC 2 cut(s) 204, 429
BstZI CGGCCG 3 cut(s) 237, 823, 912
Bsu15I ATCGAT 1 cut(s) 3397
BsuI GTATCC 1 cut(s) 1840
BsuTUI ATCGAT 1 cut(s) 3397
BtgI CCRYGG 5 cut(s) 240, 1773, 2481, 2610, 2877
BtrI CACGTC 1 cut(s) 1042
BtsCI GGATG 7 cut(s) 255, 445, 834, 1376, 2515, 3537, 3684
BtsI GCAGTG 5 cut(s) 606, 790, 1868, 2220, 3498
BtuMI TCGCGA 3 cut(s) 1133, 1179, 1197
BveI ACCTGC 2 cut(s) 1045, 2917
Cac8I GCNNGC 8 cut(s) 237, 575, 849, 853, 1314, 1450, 3027, 3064
CaiI CAGNNNCTG 2 cut(s) 816, 1060
CfoI GCGC 4 cut(s) 887, 1054, 1312, 1454
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 7 cut(s) 1257, 1606, 1690, 1892, 3306, 3495, 3579
ClaI ATCGAT 1 cut(s) 3397
CseI GACGC 3 cut(s) 706, 1068, 2025
DrdI GACNNNNNNGTC 2 cut(s) 703, 730
DseDI GACNNNNNNGTC 2 cut(s) 703, 730
EaeI YGGCCR 5 cut(s) 233, 237, 787, 823, 912
EagI CGGCCG 3 cut(s) 237, 823, 912
Eam1104I CTCTTC 4 cut(s) 169, 588, 1247, 2390
EarI CTCTTC 4 cut(s) 169, 588, 1247, 2390
EciI GGCGGA 1 cut(s) 3677
Ecl136II GAGCTC 1 cut(s) 973
EclXI CGGCCG 3 cut(s) 237, 823, 912
Eco105I TACGTA 1 cut(s) 2216
Eco130I CCWWGG 4 cut(s) 2481, 3067, 3309, 3463
Eco147I AGGCCT 1 cut(s) 2904
Eco24I GRGCYC 3 cut(s) 354, 975, 3230
Eco31I GGTCTC 4 cut(s) 654, 865, 2889, 3473
Eco47I GGWCC 4 cut(s) 1257, 1690, 1892, 3579
Eco52I CGGCCG 3 cut(s) 237, 823, 912
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 1 cut(s) 612
Eco91I GGTNACC 1 cut(s) 2761
EcoICRI GAGCTC 1 cut(s) 973
EcoO109I RGGNCCY 1 cut(s) 1690
EcoO65I GGTNACC 1 cut(s) 2761
EcoRI GAATTC 2 cut(s) 3156, 3571
EcoRII CCWGG 3 cut(s) 138, 1751, 3194
EcoT14I CCWWGG 4 cut(s) 2481, 3067, 3309, 3463
EcoT22I ATGCAT 4 cut(s) 1552, 2281, 2515, 3404
EcoT38I GRGCYC 3 cut(s) 354, 975, 3230
ErhI CCWWGG 4 cut(s) 2481, 3067, 3309, 3463
Esp3I CGTCTC 1 cut(s) 166
FaqI GGGAC 5 cut(s) 140, 1052, 2034, 3014, 3110
FauI CCCGC 2 cut(s) 856, 2255
FauNDI CATATG 1 cut(s) 2685
FbaI TGATCA 2 cut(s) 2713, 3139
FblI GTMKAC 3 cut(s) 203, 428, 2410
FokI GGATG 7 cut(s) 262, 452, 821, 1383, 2522, 3544, 3671
FriOI GRGCYC 3 cut(s) 354, 975, 3230
FseI GGCCGGCC 1 cut(s) 239
FspBI CTAG 5 cut(s) 626, 1377, 3320, 3489, 3615
GlaI GCGC 4 cut(s) 886, 1053, 1311, 1453
GsaI CCCAGC 4 cut(s) 272, 1169, 3136, 3496
GsuI CTGGAG 2 cut(s) 1292, 2912
HgaI GACGC 3 cut(s) 706, 1068, 2025
HhaI GCGC 4 cut(s) 887, 1054, 1312, 1454
Hin1I GRCGYC 5 cut(s) 704, 777, 993, 2036, 2413
Hin6I GCGC 4 cut(s) 885, 1052, 1310, 1452
HinP1I GCGC 4 cut(s) 885, 1052, 1310, 1452
HincII GTYRAC 2 cut(s) 526, 2501
HindII GTYRAC 2 cut(s) 526, 2501
HindIII AAGCTT 1 cut(s) 42
HphI GGTGA 8 cut(s) 733, 796, 1587, 1748, 1976, 2249, 2755, 3533
Hpy99I CGWCG 6 cut(s) 700, 709, 779, 995, 998, 1031
HpyCH4III ACNGT 6 cut(s) 227, 523, 748, 2163, 2919, 3101
HpyCH4IV ACGT 8 cut(s) 704, 777, 993, 1041, 2215, 2413, 3372, 3588
HpyF10VI GCNNNNNNNGC 8 cut(s) 596, 621, 706, 3063, 3356, 3509, 3541, 3611
HpySE526I ACGT 8 cut(s) 704, 777, 993, 1041, 2215, 2413, 3372, 3588
Hsp92I GRCGYC 5 cut(s) 704, 777, 993, 2036, 2413
HspAI GCGC 4 cut(s) 885, 1052, 1310, 1452
Kpn2I TCCGGA 1 cut(s) 3363
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Ksp22I TGATCA 2 cut(s) 2713, 3139
LguI GCTCTTC 1 cut(s) 2390
Lsp1109I GCAGC 5 cut(s) 1127, 1753, 1781, 2916, 3276
MaeI CTAG 5 cut(s) 626, 1377, 3320, 3489, 3615
MaeII ACGT 8 cut(s) 704, 777, 993, 1041, 2215, 2413, 3372, 3588
MaeIII GTNAC 8 cut(s) 95, 227, 517, 721, 1822, 2761, 2974, 3589
MbiI CCGCTC 1 cut(s) 851
MfeI CAATTG 2 cut(s) 1974, 3006
MflI RGATCY 3 cut(s) 1931, 2587, 3617
MhlI GDGCHC 8 cut(s) 30, 275, 336, 354, 746, 975, 1903, 3230
MlyI GAGTC 4 cut(s) 917, 1103, 2996, 3554
MmeI TCCRAC 5 cut(s) 1437, 1606, 2217, 2341, 2901
Mph1103I ATGCAT 4 cut(s) 1552, 2281, 2515, 3404
MroI TCCGGA 1 cut(s) 3363
MroNI GCCGGC 1 cut(s) 235
MseI TTAA 6 cut(s) 6, 1809, 2433, 2441, 2466, 3515
MslI CAYNNNNRTG 4 cut(s) 1761, 2078, 3543, 3549
MspA1I CMGCKG 2 cut(s) 1015, 1165
MunI CAATTG 2 cut(s) 1974, 3006
Mva1269I GAATGC 1 cut(s) 1345
MvaI CCWGG 3 cut(s) 140, 1753, 3196
MvnI CGCG 5 cut(s) 887, 1133, 1179, 1197, 1454
MwoI GCNNNNNNNGC 8 cut(s) 596, 621, 706, 3063, 3356, 3509, 3541, 3611
NaeI GCCGGC 1 cut(s) 237
NciI CCSGG 7 cut(s) 354, 473, 857, 911, 1084, 1351, 1408
NcoI CCATGG 1 cut(s) 2481
NdeI CATATG 1 cut(s) 2685
NgoMIV GCCGGC 1 cut(s) 235
NlaIV GGNNCC 5 cut(s) 1161, 1211, 1317, 1691, 3435
NmeAIII GCCGAG 1 cut(s) 3098
NmuCI GTSAC 7 cut(s) 95, 227, 517, 721, 1822, 2761, 2974
NruI TCGCGA 3 cut(s) 1133, 1179, 1197
NsiI ATGCAT 4 cut(s) 1552, 2281, 2515, 3404
NspI RCATGY 7 cut(s) 1520, 1766, 1841, 2170, 2180, 3190, 3542
NspV TTCGAA 2 cut(s) 325, 1112
PceI AGGCCT 1 cut(s) 2904
PciI ACATGT 1 cut(s) 3538
PciSI GCTCTTC 1 cut(s) 2390
PcsI WCGNNNNNNNCGW 6 cut(s) 792, 912, 987, 1236, 1451, 3070
PctI GAATGC 1 cut(s) 1345
PdiI GCCGGC 1 cut(s) 237
PflFI GACNNNGTC 2 cut(s) 926, 938
PflMI CCANNNNNTGG 2 cut(s) 2140, 2360
PfoI TCCNGGA 1 cut(s) 471
PleI GAGTC 4 cut(s) 917, 1102, 2996, 3554
PpsI GAGTC 4 cut(s) 917, 1102, 2996, 3554
Ppu21I YACGTR 2 cut(s) 2216, 3589
PpuMI RGGWCCY 1 cut(s) 1690
PscI ACATGT 1 cut(s) 3538
PsiI TTATAA 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 975
Psp5II RGGWCCY 1 cut(s) 1690
Psp6I CCWGG 3 cut(s) 138, 1751, 3194
PspEI GGTNACC 1 cut(s) 2761
PspFI CCCAGC 4 cut(s) 268, 1165, 3132, 3492
PspGI CCWGG 3 cut(s) 138, 1751, 3194
PspN4I GGNNCC 5 cut(s) 1161, 1211, 1317, 1691, 3435
PspPI GGNCC 7 cut(s) 1257, 1606, 1690, 1892, 3306, 3495, 3579
PspPPI RGGWCCY 1 cut(s) 1690
PsrI GAACNNNNNNTAC 4 cut(s) 1193, 1225, 3036, 3068
PstI CTGCAG 4 cut(s) 1284, 1771, 1863, 2914
PstNI CAGNNNCTG 2 cut(s) 816, 1060
PsuI RGATCY 3 cut(s) 1931, 2587, 3617
PsyI GACNNNGTC 2 cut(s) 926, 938
RigI GGCCGGCC 1 cut(s) 239
RruI TCGCGA 3 cut(s) 1133, 1179, 1197
RseI CAYNNNNRTG 4 cut(s) 1761, 2078, 3543, 3549
SacI GAGCTC 1 cut(s) 975
SapI GCTCTTC 1 cut(s) 2390
SaqAI TTAA 6 cut(s) 6, 1809, 2433, 2441, 2466, 3515
Sau96I GGNCC 7 cut(s) 1257, 1606, 1690, 1892, 3306, 3495, 3579
SbfI CCTGCAGG 1 cut(s) 2914
ScaI AGTACT 1 cut(s) 3103
SchI GAGTC 4 cut(s) 917, 1103, 2996, 3554
SdaI CCTGCAGG 1 cut(s) 2914
SduI GDGCHC 8 cut(s) 30, 275, 336, 354, 746, 975, 1903, 3230
SfcI CTRYAG 5 cut(s) 1280, 1767, 1859, 2910, 3438
SfuI TTCGAA 2 cut(s) 325, 1112
SinI GGWCC 4 cut(s) 1257, 1690, 1892, 3579
SmiMI CAYNNNNRTG 4 cut(s) 1761, 2078, 3543, 3549
SmlI CTYRAG 1 cut(s) 3621
SmoI CTYRAG 1 cut(s) 3621
SnaBI TACGTA 1 cut(s) 2216
SpeI ACTAGT 1 cut(s) 3319
Sse8387I CCTGCAGG 1 cut(s) 2914
SseBI AGGCCT 1 cut(s) 2904
SspMI CTAG 5 cut(s) 626, 1377, 3320, 3489, 3615
SstI GAGCTC 1 cut(s) 975
StuI AGGCCT 1 cut(s) 2904
StyI CCWWGG 4 cut(s) 2481, 3067, 3309, 3463
TaaI ACNGT 6 cut(s) 227, 523, 748, 2163, 2919, 3101
TaiI ACGT 8 cut(s) 707, 780, 996, 1044, 2218, 2416, 3375, 3591
TaqII GACCGA 1 cut(s) 1274
TatI WGTACW 4 cut(s) 1840, 1986, 2082, 3101
TauI GCSGC 6 cut(s) 695, 885, 1015, 1049, 1052, 3359
Tru1I TTAA 6 cut(s) 6, 1809, 2433, 2441, 2466, 3515
Tru9I TTAA 6 cut(s) 6, 1809, 2433, 2441, 2466, 3515
TseFI GTSAC 7 cut(s) 95, 227, 517, 721, 1822, 2761, 2974
TseI GCWGC 5 cut(s) 1140, 1766, 1769, 2929, 3289
Tsp45I GTSAC 7 cut(s) 95, 227, 517, 721, 1822, 2761, 2974
TspDTI ATGAA 3 cut(s) 2321, 2685, 3564
TspGWI ACGGA 7 cut(s) 38, 189, 740, 916, 1311, 2599, 2982
Tth111I GACNNNGTC 2 cut(s) 926, 938
Van91I CCANNNNNTGG 2 cut(s) 2140, 2360
VpaK11BI GGWCC 4 cut(s) 1257, 1690, 1892, 3579
XapI RAATTY 7 cut(s) 1235, 1671, 2461, 2651, 2692, 3156, 3571
XceI RCATGY 7 cut(s) 1520, 1766, 1841, 2170, 2180, 3190, 3542
XmiI GTMKAC 3 cut(s) 203, 428, 2410
XspI CTAG 5 cut(s) 626, 1377, 3320, 3489, 3615
ZraI GACGTC 4 cut(s) 705, 778, 994, 2414
ZrmI AGTACT 1 cut(s) 3103
Zsp2I ATGCAT 4 cut(s) 1552, 2281, 2515, 3404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.