Rh5BG027100

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006279
Physical Location & Seq
Forward (+)
1906307 .. 1911334
5028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG027100.1

Sequence Viewer

Length: 2772 bp
ATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCACTGAGATCGGAGGTGACGGACAAGTCGCCGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACGTCATCAACGGCCACCGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGCGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGATCTCGTCTTCTGCTCCTCCGATCAGAATGAGCCGGAGCTCCAAGACGACCAAAACGACGTCGTTTTCAGATTCGAGCCTCTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCGCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGAGGCTTTTCTGGAGGCTCTGCAGAGTGGGGAGAGCGGAGGAGGGTTTGCGCTGGCTCCGGCTGGTGGAAATTACGAGAATGCCCCTTCCGGGTTGAAGGGAGATAGTGGATGCTCTAGTCTGTCTGTTGTTCCGATAGAGGTTTCCCGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCAGCTTGCGCGTTGGAGAAGAAGGTGGATAATGGGATTCTTGTGTTTGGTGGATTCGGAGGCATTGGAAGACATGCGAGGAGAAATCAGTGTTTGCTGGTTGATGCATTGTCTGGTAGTTTGAGAGGGATTAGCGTAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGACATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCGCCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAGGATCAAAGATATATGTGTTTGGTGGACTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGATACAGATAATCTGCAGTGGAGAGAGACAGTTGTCACTGGGGAAGGTCCGTGTGCCCGACATTCACACTCAATGGTGGCATCGGGATCTCAGCTATATGTGTTCGGAGGATATGATGGTGAGGAAGCAATTGGAGACTTGTACAGGTTTGATACTGAAAAATCTAAATGTAGATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCACACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACATTGCCAAGAGTTGCCAATACTTGATCTACGGTTGTGCATGTGGAGGCATGTGAAACTAGAATCCACTGGTGAAGATTTGTTTGTACGTAGCACTGCCAATGTTGTTGGTGATGATCTTGTTATGATTGGTGGTGGGGCATCTTGTTACGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCCCTGAAGCTTAGAGATGATAAACCACTTGTTAGAGAACGGCATACTGACCAAATTGATATCGTGAGGAGCGAAAAGAGTAGACATCCACAGGTTCAAAATGTTAAAACATTAACCGAAGCTCCTGACCTAAATTTTAAGAGTGAATTACCCATGGCTGATGGCATTGGTCAACAGGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGGAAGGACATACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCTTGGAAGGTGGTTTACATATTTGTTTCCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCGGCATCATATGGCAAATTCATTTGAAGGACAGAGTGGTCACATTTGTAAGCCAGATATAGGAAAGGAATGTTCGATTGATGAGGTCACCTGTTCAAAAGCCTTAGACATTCTAAAGGAATGTGGTGCCATGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAGTGATGCTGTGGGGTCTCTGCTAGAGGATAAAGGCCTACCTGCAGGACTGTTGGAGGAGCTACCAACCAGATGGGAGCGACTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCCGTTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGCTGTTGCCAAATCAGTAAATGCTGTTCGCCTTGCTCGCCAAGGCCGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGCTGGGTTGATCATCGTGAAAATGGAATTCTCTATTCTTTTGATGCTACTAAGTGTATGTTCTCCTGGGGCAACCTTTCTGAGAAGCTCCGCATGGGCTCTCTGGATTGTAGAGATGAGATCATTGTGGATTTATTTGCTGGAATTGGATATTTTGTGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGCGGCACTCCGGAGAAACGTACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGGACAACGGCACCTACAGTATGA

Protein Analysis

923

Amino Acids

101.55

Weight (kDa)

7.51

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 205 4.4e-67 Methyltransferase TYW3
Beta-prop_FBX42 PF13415 255 - 349 2.7e-11 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 257 - 418 7.6e-35 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 270 - 430 1.9e-15 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 273 - 352 1.2e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 301 - 400 3.1e-11 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 304 - 394 8.5e-19 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 308 - 543 1.9e-26 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 310 - 350 4.6e-08 Kelch motif
Kelch_2 PF07646 310 - 351 2.5e-06 Kelch motif
Beta-prop_Calicin PF13964 322 - 449 1.5e-08 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 337 - 406 2.5e-10 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 361 - 434 1.2e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 361 - 401 8.2e-09 Kelch motif
Kelch_2 PF07646 361 - 402 2.2e-08 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 365 - 536 1.8e-39 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 382 - 497 2e-20 FBX42, beta-propeller domain
Kelch_HCF PF13854 396 - 482 4.3e-08 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 398 - 447 2.3e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 411 - 449 1e-07 Kelch motif
Kelch_2 PF07646 412 - 448 1.8e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 424 - 538 2.3e-13 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 741 - 818 1.4e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 823 - 919 1.7e-24 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 34, 61, 2037
AatII GACGTC 3 cut(s) 38, 111, 327
Acc36I ACCTGC 2 cut(s) 376, 2242
AccB1I GGYRCC 2 cut(s) 2126, 2758
AccBSI CCGCTC 2 cut(s) 182, 627
AccI GTMKAC 1 cut(s) 1735
AccII CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
AccIII TCCGGA 1 cut(s) 2688
AclWI GGATC 4 cut(s) 242, 986, 1120, 1270
AcoI YGGCCR 3 cut(s) 118, 154, 243
AcsI RAATTY 6 cut(s) 566, 1002, 1786, 1976, 2017, 2481
AcuI CTGAAG 1 cut(s) 1679
AcyI GRCGYC 4 cut(s) 35, 108, 324, 1367
AfaI GTAC 8 cut(s) 1173, 1319, 1415, 1545, 1618, 2416, 2428, 2700
AfiI CCNNNNNNNGG 7 cut(s) 186, 656, 681, 935, 1389, 2060, 2755
AhlI ACTAGT 1 cut(s) 2644
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 2 cut(s) 1082, 2519
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 5 cut(s) 196, 528, 1196, 1305, 2214
AlwI GGATC 4 cut(s) 242, 986, 1120, 1270
AlwNI CAGNNNCTG 2 cut(s) 147, 391
Ama87I CYCGRG 1 cut(s) 738
Aor13HI TCCGGA 1 cut(s) 2688
AoxI GGCC 9 cut(s) 118, 154, 243, 573, 937, 2227, 2348, 2395, 2631
ApeKI GCWGC 4 cut(s) 471, 1097, 1100, 2614
ApoI RAATTY 6 cut(s) 566, 1002, 1786, 1976, 2017, 2481
AspLEI GCGC 4 cut(s) 218, 385, 643, 785
AspS9I GGNCC 5 cut(s) 588, 937, 1021, 1223, 2631
AsuC2I CCSGG 6 cut(s) 188, 242, 415, 682, 739, 740
AsuHPI GGTGA 8 cut(s) 64, 127, 918, 1079, 1307, 1541, 1580, 2080
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 738
AvaII GGWCC 3 cut(s) 588, 1021, 1223
BaeGI GKGCMC 2 cut(s) 77, 1234
BanI GGYRCC 2 cut(s) 2126, 2758
BanII GRGCYC 2 cut(s) 306, 2555
BarI GAAGNNNNNNTAC 2 cut(s) 1932, 1964
BbsI GAAGAC 2 cut(s) 265, 850
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 2337
BbvI GCAGC 4 cut(s) 458, 1084, 1112, 2601
BccI CCATC 5 cut(s) 173, 1286, 1344, 1808, 2259
BceAI ACGGC 5 cut(s) 133, 341, 960, 1088, 1709
BciT130I CCWGG 2 cut(s) 1084, 2521
BciVI GTATCC 1 cut(s) 1171
BclI TGATCA 1 cut(s) 2464
BcnI CCSGG 6 cut(s) 188, 242, 415, 682, 739, 740
BcoDI GTCTC 5 cut(s) 196, 528, 1196, 1305, 2214
BcuI ACTAGT 1 cut(s) 2644
BfaI CTAG 4 cut(s) 708, 1517, 2216, 2645
BfmI CTRYAG 5 cut(s) 611, 1098, 1190, 2235, 2763
BfuAI ACCTGC 2 cut(s) 376, 2242
BfuI GTATCC 1 cut(s) 1171
BglII AGATCT 1 cut(s) 1912
BisI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
BlsI GCNGC 9 cut(s) 25, 215, 379, 382, 473, 1099, 1102, 2616, 2683
BmcAI AGTACT 1 cut(s) 2428
Bme1390I CCNGG 8 cut(s) 188, 242, 415, 682, 739, 740, 1084, 2521
Bme18I GGWCC 3 cut(s) 588, 1021, 1223
BmeT110I CYCGRG 1 cut(s) 738
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 5 cut(s) 588, 937, 1021, 1223, 2631
BmiI GGNNCC 6 cut(s) 492, 542, 648, 1022, 2128, 2760
BmrFI CCNGG 8 cut(s) 188, 242, 415, 682, 739, 740, 1084, 2521
BmrI ACTGGG 1 cut(s) 1224
BmuI ACTGGG 1 cut(s) 1224
BoxI GACNNNNGTC 1 cut(s) 1208
BpiI GAAGAC 2 cut(s) 265, 850
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 2337
Bpu14I TTCGAA 1 cut(s) 443
BpuMI CCSGG 6 cut(s) 188, 242, 415, 682, 739, 740
Bsa29I ATCGAT 1 cut(s) 2722
BsaAI YACGTR 1 cut(s) 1547
BsaHI GRCGYC 4 cut(s) 35, 108, 324, 1367
BsaI GGTCTC 2 cut(s) 196, 2214
BsaJI CCNNGG 9 cut(s) 591, 738, 1082, 1104, 1806, 1935, 2392, 2520, 2634
BsaWI WCCGGW 3 cut(s) 579, 2688, 2748
BsaXI ACNNNNNCTCC 4 cut(s) 1759, 1789, 2271, 2301
Bsc4I CCNNNNNNNGG 7 cut(s) 186, 656, 681, 935, 1389, 2060, 2755
Bse1I ACTGG 6 cut(s) 1179, 1219, 1448, 1531, 2297, 2416
Bse3DI GCAATG 1 cut(s) 1459
BseAI TCCGGA 1 cut(s) 2688
BseBI CCWGG 2 cut(s) 1084, 2521
BseCI ATCGAT 1 cut(s) 2722
BseDI CCNNGG 9 cut(s) 591, 738, 1082, 1104, 1806, 1935, 2392, 2520, 2634
BseGI GGATG 4 cut(s) 165, 707, 1738, 1840
BseLI CCNNNNNNNGG 7 cut(s) 186, 656, 681, 935, 1389, 2060, 2755
BseMI GCAATG 1 cut(s) 1459
BseMII CTCAG 7 cut(s) 32, 188, 987, 998, 1280, 2328, 2526
BseNI ACTGG 6 cut(s) 1179, 1219, 1448, 1531, 2297, 2416
BseRI GAGGAG 8 cut(s) 92, 271, 645, 764, 868, 1735, 2264, 2354
BseSI GKGCMC 2 cut(s) 77, 1234
BseX3I CGGCCG 2 cut(s) 154, 243
BseXI GCAGC 4 cut(s) 458, 1084, 1112, 2601
BseYI CCCAGC 2 cut(s) 496, 2457
BsgI GTGCAG 1 cut(s) 385
Bsh1236I CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
Bsh1285I CGRYCG 2 cut(s) 157, 246
BshFI GGCC 9 cut(s) 120, 156, 245, 575, 939, 2229, 2350, 2397, 2633
BshNI GGYRCC 2 cut(s) 2126, 2758
BshVI ATCGAT 1 cut(s) 2722
BsiEI CGRYCG 2 cut(s) 157, 246
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 738
BslFI GGGAC 4 cut(s) 383, 1365, 2339, 2435
BslI CCNNNNNNNGG 7 cut(s) 186, 656, 681, 935, 1389, 2060, 2755
BsmAI GTCTC 5 cut(s) 196, 528, 1196, 1305, 2214
BsmFI GGGAC 4 cut(s) 383, 1365, 2339, 2435
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 9 cut(s) 120, 156, 245, 575, 939, 2229, 2350, 2397, 2633
Bso31I GGTCTC 2 cut(s) 196, 2214
BsoBI CYCGRG 1 cut(s) 738
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 4 cut(s) 77, 306, 1234, 2555
Bsp13I TCCGGA 1 cut(s) 2688
Bsp1407I TGTACA 2 cut(s) 1317, 1413
Bsp19I CCATGG 1 cut(s) 1806
Bsp68I TCGCGA 3 cut(s) 464, 510, 528
BspANI GGCC 9 cut(s) 120, 156, 245, 575, 939, 2229, 2350, 2397, 2633
BspCNI CTCAG 7 cut(s) 33, 187, 988, 999, 1279, 2329, 2527
BspDI ATCGAT 1 cut(s) 2722
BspEI TCCGGA 1 cut(s) 2688
BspFNI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
BspLI GGNNCC 6 cut(s) 492, 542, 648, 1022, 2128, 2760
BspMAI CTGCAG 4 cut(s) 615, 1102, 1194, 2239
BspMI ACCTGC 2 cut(s) 376, 2242
BspPI GGATC 4 cut(s) 242, 986, 1120, 1270
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 2 cut(s) 2126, 2758
BspTNI GGTCTC 2 cut(s) 196, 2214
BsrBI CCGCTC 2 cut(s) 182, 627
BsrDI GCAATG 1 cut(s) 1459
BsrGI TGTACA 2 cut(s) 1317, 1413
BsrI ACTGG 6 cut(s) 1179, 1219, 1448, 1531, 2297, 2416
BssECI CCNNGG 9 cut(s) 591, 738, 1082, 1104, 1806, 1935, 2392, 2520, 2634
BssNI GRCGYC 4 cut(s) 35, 108, 324, 1367
BssT1I CCWWGG 4 cut(s) 1806, 1935, 2392, 2634
Bst2UI CCWGG 2 cut(s) 1084, 2521
Bst4CI ACNGT 6 cut(s) 79, 1207, 1491, 2244, 2426, 2767
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 4 cut(s) 35, 108, 324, 1367
BstAUI TGTACA 2 cut(s) 1317, 1413
BstBAI YACGTR 1 cut(s) 1547
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 6 cut(s) 180, 184, 645, 781, 2352, 2389
BstDSI CCRYGG 2 cut(s) 1104, 1806
BstEII GGTNACC 1 cut(s) 2086
BstF5I GGATG 4 cut(s) 165, 707, 1738, 1840
BstFNI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
BstHHI GCGC 4 cut(s) 218, 385, 643, 785
BstMAI GTCTC 5 cut(s) 196, 528, 1196, 1305, 2214
BstMCI CGRYCG 2 cut(s) 157, 246
BstMWI GCNNNNNNNGC 2 cut(s) 2388, 2681
BstNI CCWGG 2 cut(s) 1084, 2521
BstNSI RCATGY 5 cut(s) 851, 1097, 1172, 1501, 1511
BstPAI GACNNNNGTC 1 cut(s) 1208
BstPI GGTNACC 1 cut(s) 2086
BstSCI CCNGG 8 cut(s) 186, 240, 413, 680, 737, 738, 1082, 2519
BstSFI CTRYAG 5 cut(s) 611, 1098, 1190, 2235, 2763
BstSLI GKGCMC 2 cut(s) 77, 1234
BstSNI TACGTA 1 cut(s) 1547
BstUI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
BstV1I GCAGC 4 cut(s) 458, 1084, 1112, 2601
BstV2I GAAGAC 2 cut(s) 265, 850
BstX2I RGATCY 2 cut(s) 1262, 1912
BstXI CCANNNNNNTGG 2 cut(s) 2137, 2265
BstYI RGATCY 2 cut(s) 1262, 1912
BstZI CGGCCG 2 cut(s) 154, 243
Bsu15I ATCGAT 1 cut(s) 2722
BsuI GTATCC 1 cut(s) 1171
BsuRI GGCC 9 cut(s) 120, 156, 245, 575, 939, 2229, 2350, 2397, 2633
BsuTUI ATCGAT 1 cut(s) 2722
BtgI CCRYGG 2 cut(s) 1104, 1806
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 4 cut(s) 165, 707, 1738, 1840
BtsI GCAGTG 2 cut(s) 1199, 1551
BtsIMutI CAGTG 8 cut(s) 38, 869, 1199, 1212, 1524, 1551, 2304, 2722
BtuMI TCGCGA 3 cut(s) 464, 510, 528
BveI ACCTGC 2 cut(s) 376, 2242
Cac8I GCNNGC 6 cut(s) 180, 184, 645, 781, 2352, 2389
CaiI CAGNNNCTG 2 cut(s) 147, 391
CfoI GCGC 4 cut(s) 218, 385, 643, 785
Cfr13I GGNCC 5 cut(s) 588, 937, 1021, 1223, 2631
Cfr9I CCCGGG 1 cut(s) 738
ClaI ATCGAT 1 cut(s) 2722
CseI GACGC 3 cut(s) 37, 399, 1356
Csp6I GTAC 8 cut(s) 1172, 1318, 1414, 1544, 1617, 2415, 2427, 2699
CviQI GTAC 8 cut(s) 1172, 1318, 1414, 1544, 1617, 2415, 2427, 2699
DrdI GACNNNNNNGTC 3 cut(s) 34, 61, 2037
DseDI GACNNNNNNGTC 3 cut(s) 34, 61, 2037
EaeI YGGCCR 3 cut(s) 118, 154, 243
EagI CGGCCG 2 cut(s) 154, 243
Eam1104I CTCTTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 578
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 2 cut(s) 154, 243
Eco105I TACGTA 1 cut(s) 1547
Eco130I CCWWGG 4 cut(s) 1806, 1935, 2392, 2634
Eco147I AGGCCT 1 cut(s) 2229
Eco24I GRGCYC 2 cut(s) 306, 2555
Eco31I GGTCTC 2 cut(s) 196, 2214
Eco32I GATATC 1 cut(s) 1714
Eco47I GGWCC 3 cut(s) 588, 1021, 1223
Eco52I CGGCCG 2 cut(s) 154, 243
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 1679
Eco88I CYCGRG 1 cut(s) 738
Eco91I GGTNACC 1 cut(s) 2086
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 1021
EcoO65I GGTNACC 1 cut(s) 2086
EcoRI GAATTC 1 cut(s) 2481
EcoRII CCWGG 2 cut(s) 1082, 2519
EcoRV GATATC 1 cut(s) 1714
EcoT14I CCWWGG 4 cut(s) 1806, 1935, 2392, 2634
EcoT22I ATGCAT 3 cut(s) 883, 1840, 2729
EcoT38I GRGCYC 2 cut(s) 306, 2555
ErhI CCWWGG 4 cut(s) 1806, 1935, 2392, 2634
FaqI GGGAC 4 cut(s) 383, 1365, 2339, 2435
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 2010
FbaI TGATCA 1 cut(s) 2464
FblI GTMKAC 1 cut(s) 1735
Fnu4HI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
FokI GGATG 4 cut(s) 152, 714, 1725, 1847
FriOI GRGCYC 2 cut(s) 306, 2555
Fsp4HI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
FspBI CTAG 4 cut(s) 708, 1517, 2216, 2645
GlaI GCGC 4 cut(s) 217, 384, 642, 784
GluI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
GsaI CCCAGC 2 cut(s) 500, 2461
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 9 cut(s) 120, 156, 245, 575, 939, 2229, 2350, 2397, 2633
HgaI GACGC 3 cut(s) 37, 399, 1356
HhaI GCGC 4 cut(s) 218, 385, 643, 785
Hin1I GRCGYC 4 cut(s) 35, 108, 324, 1367
Hin6I GCGC 4 cut(s) 216, 383, 641, 783
HinP1I GCGC 4 cut(s) 216, 383, 641, 783
HincII GTYRAC 1 cut(s) 1826
HindII GTYRAC 1 cut(s) 1826
HindIII AAGCTT 1 cut(s) 1661
HphI GGTGA 8 cut(s) 64, 127, 918, 1079, 1307, 1541, 1580, 2080
Hpy166II GTNNAC 7 cut(s) 82, 1136, 1414, 1736, 1826, 1930, 1949
Hpy8I GTNNAC 7 cut(s) 82, 1136, 1414, 1736, 1826, 1930, 1949
Hpy99I CGWCG 5 cut(s) 31, 110, 326, 329, 362
HpyCH4III ACNGT 6 cut(s) 79, 1207, 1491, 2244, 2426, 2767
HpyCH4IV ACGT 6 cut(s) 35, 108, 324, 372, 1546, 2697
HpyF10VI GCNNNNNNNGC 2 cut(s) 2388, 2681
HpySE526I ACGT 6 cut(s) 35, 108, 324, 372, 1546, 2697
Hsp92I GRCGYC 4 cut(s) 35, 108, 324, 1367
HspAI GCGC 4 cut(s) 216, 383, 641, 783
Kpn2I TCCGGA 1 cut(s) 2688
Ksp22I TGATCA 1 cut(s) 2464
Lsp1109I GCAGC 4 cut(s) 458, 1084, 1112, 2601
MaeI CTAG 4 cut(s) 708, 1517, 2216, 2645
MaeII ACGT 6 cut(s) 35, 108, 324, 372, 1546, 2697
MaeIII GTNAC 8 cut(s) 36, 52, 1153, 1210, 1604, 2039, 2086, 2299
MbiI CCGCTC 2 cut(s) 182, 627
MboII GAAGA 8 cut(s) 211, 265, 595, 805, 855, 1048, 1152, 1544
MfeI CAATTG 2 cut(s) 1305, 2331
MflI RGATCY 2 cut(s) 1262, 1912
MhlI GDGCHC 4 cut(s) 77, 306, 1234, 2555
MlyI GAGTC 3 cut(s) 248, 434, 2321
MmeI TCCRAC 3 cut(s) 768, 937, 2226
Mph1103I ATGCAT 3 cut(s) 883, 1840, 2729
MroI TCCGGA 1 cut(s) 2688
MseI TTAA 4 cut(s) 1140, 1758, 1766, 1791
MslI CAYNNNNRTG 2 cut(s) 1092, 1409
MspA1I CMGCKG 1 cut(s) 496
MspR9I CCNGG 8 cut(s) 188, 242, 415, 682, 739, 740, 1084, 2521
MunI CAATTG 2 cut(s) 1305, 2331
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 2 cut(s) 1084, 2521
MvnI CGCG 6 cut(s) 126, 218, 464, 510, 528, 785
MwoI GCNNNNNNNGC 2 cut(s) 2388, 2681
NciI CCSGG 6 cut(s) 188, 242, 415, 682, 739, 740
NcoI CCATGG 1 cut(s) 1806
NdeI CATATG 1 cut(s) 2010
NlaIV GGNNCC 6 cut(s) 492, 542, 648, 1022, 2128, 2760
NmeAIII GCCGAG 1 cut(s) 2423
NmuCI GTSAC 7 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299
NruI TCGCGA 3 cut(s) 464, 510, 528
NsiI ATGCAT 3 cut(s) 883, 1840, 2729
NspI RCATGY 5 cut(s) 851, 1097, 1172, 1501, 1511
NspV TTCGAA 1 cut(s) 443
PceI AGGCCT 1 cut(s) 2229
PcsI WCGNNNNNNNCGW 6 cut(s) 123, 243, 318, 567, 1722, 2395
PctI GAATGC 1 cut(s) 676
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 9 cut(s) 25, 215, 379, 382, 473, 1099, 1102, 2616, 2683
PleI GAGTC 3 cut(s) 248, 433, 2321
PpsI GAGTC 3 cut(s) 248, 433, 2321
Ppu21I YACGTR 1 cut(s) 1547
PpuMI RGGWCCY 1 cut(s) 1021
PshAI GACNNNNGTC 1 cut(s) 1208
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 1021
Psp6I CCWGG 2 cut(s) 1082, 2519
PspEI GGTNACC 1 cut(s) 2086
PspFI CCCAGC 2 cut(s) 496, 2457
PspGI CCWGG 2 cut(s) 1082, 2519
PspN4I GGNNCC 6 cut(s) 492, 542, 648, 1022, 2128, 2760
PspPI GGNCC 5 cut(s) 588, 937, 1021, 1223, 2631
PspPPI RGGWCCY 1 cut(s) 1021
PsrI GAACNNNNNNTAC 4 cut(s) 524, 556, 2361, 2393
PstI CTGCAG 4 cut(s) 615, 1102, 1194, 2239
PstNI CAGNNNCTG 2 cut(s) 147, 391
PsuI RGATCY 2 cut(s) 1262, 1912
PsyI GACNNNGTC 1 cut(s) 257
RruI TCGCGA 3 cut(s) 464, 510, 528
RsaI GTAC 8 cut(s) 1173, 1319, 1415, 1545, 1618, 2416, 2428, 2700
RsaNI GTAC 8 cut(s) 1172, 1318, 1414, 1544, 1617, 2415, 2427, 2699
RseI CAYNNNNRTG 2 cut(s) 1092, 1409
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 4 cut(s) 1140, 1758, 1766, 1791
SatI GCNGC 9 cut(s) 24, 214, 378, 381, 472, 1098, 1101, 2615, 2682
Sau96I GGNCC 5 cut(s) 588, 937, 1021, 1223, 2631
SbfI CCTGCAGG 1 cut(s) 2239
ScaI AGTACT 1 cut(s) 2428
SchI GAGTC 3 cut(s) 248, 434, 2321
ScrFI CCNGG 8 cut(s) 188, 242, 415, 682, 739, 740, 1084, 2521
SdaI CCTGCAGG 1 cut(s) 2239
SduI GDGCHC 4 cut(s) 77, 306, 1234, 2555
SfcI CTRYAG 5 cut(s) 611, 1098, 1190, 2235, 2763
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 3 cut(s) 588, 1021, 1223
SmaI CCCGGG 1 cut(s) 740
SmiMI CAYNNNNRTG 2 cut(s) 1092, 1409
SnaBI TACGTA 1 cut(s) 1547
SpeI ACTAGT 1 cut(s) 2644
Sse8387I CCTGCAGG 1 cut(s) 2239
SseBI AGGCCT 1 cut(s) 2229
SspMI CTAG 4 cut(s) 708, 1517, 2216, 2645
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 2229
StyD4I CCNGG 8 cut(s) 186, 240, 413, 680, 737, 738, 1082, 2519
StyI CCWWGG 4 cut(s) 1806, 1935, 2392, 2634
TaaI ACNGT 6 cut(s) 79, 1207, 1491, 2244, 2426, 2767
TaiI ACGT 6 cut(s) 38, 111, 327, 375, 1549, 2700
TaqII GACCGA 1 cut(s) 605
TatI WGTACW 4 cut(s) 1171, 1317, 1413, 2426
TauI GCSGC 5 cut(s) 26, 216, 380, 383, 2684
Tru1I TTAA 4 cut(s) 1140, 1758, 1766, 1791
Tru9I TTAA 4 cut(s) 1140, 1758, 1766, 1791
TscAI CASTG 8 cut(s) 45, 869, 1199, 1219, 1531, 1558, 2304, 2722
TseFI GTSAC 7 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299
TseI GCWGC 4 cut(s) 471, 1097, 1100, 2614
Tsp45I GTSAC 7 cut(s) 36, 52, 1153, 1210, 2039, 2086, 2299
TspDTI ATGAA 3 cut(s) 1652, 2010, 2147
TspGWI ACGGA 4 cut(s) 71, 247, 1215, 2307
TspMI CCCGGG 1 cut(s) 738
TspRI CASTG 8 cut(s) 45, 869, 1199, 1219, 1531, 1558, 2304, 2722
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 588, 1021, 1223
XapI RAATTY 6 cut(s) 566, 1002, 1786, 1976, 2017, 2481
XceI RCATGY 5 cut(s) 851, 1097, 1172, 1501, 1511
XmaI CCCGGG 1 cut(s) 738
XmiI GTMKAC 1 cut(s) 1735
XspI CTAG 4 cut(s) 708, 1517, 2216, 2645
ZraI GACGTC 3 cut(s) 36, 109, 325
ZrmI AGTACT 1 cut(s) 2428
Zsp2I ATGCAT 3 cut(s) 883, 1840, 2729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.