Rw0G010910

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00470
OG0006279
Physical Location & Seq
Forward (+)
17845 .. 20830
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010910.1

Sequence Viewer

Length: 2241 bp
ATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCACTGAGATCGGAGCAAGAAGAAAGCCTCCGGCGGCGGAATGAGCCGGAGCTCCAAGACGACCAAAACGACATCGTTTTCAGATTCGAGCCGCTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCGCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGAGGCTTTTCTGGAGGCTCTGCGGAGTGAGAGCGGAGAGTTTATGGCTCCGGCTGGTGGAAATTACGAGAATGCCTCTTCCGGGTTGAAGGGAGATAGTGGATGCTCTAGTCTGTCTGTTGTTCCGATAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCGGCTTGCTCATTGGTGAAGAAGGCAGATAATGGGATTCTTGTGTTTGGTGGATTTGGAGGCATTGGAAGACATGCGAGAAGAAATCAGTGTTTGCTGGTTGATACATTGTCTGGTAGTTTGAAAGGGATTAGCGTAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGAGATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCGCCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAGGATCAAAGATATATGTGTTTGGCGGACTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGATACAGATAATCTGCAGTGGAGAGAGATAGTTGTCAGTGAGGAAGGTCCGTGTGCCCGACATTCACACTCAATGGTGGCATCGGGATCTCAGCTATATATGTTTGGAGGATATGATGGTGAGGAAGCAATTGGAGACTTGTACAGGTTTGATAGTGAAAAATCTAAATGTAAATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCACACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACGTTGCCAAGAGTTGGCAATACTTGATTTACGACTGTGCATGTGGAGGCATGTGAAACTGGAATCCAACGGCGAAGATTTGTTTGTACGTAGCACTGCCAATGTTTTTGGTGATGATCTTGTTATGATTGGTGGCGGGGCATCTTGCTATGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCTCTGATGCTGAGAGATGATAATCGTAAACCACTCGTTGGAGAACTGCATACTGACCAAATTGATATTGTGAAGAGCGAAAAGAGTAGACATCCACAGGTTCAAAATGTTAAAACATTAACTGAAGCTCCTGACCTAAATTTTAAGAGTGAATTACCCATGGCTGATGGCATTGGTCAACAGGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGGAAGGACATACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCGTGGAAGGTGGTTTACATATTTGTTTCCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCAGCATCATATGGCAAATTCATTTGAAGGACAGAGTGATCACATTTGTAAGCCAGATATAGGAAAGGAATGTTCGATTGATGAGGTCACCTGTTCAACAGCCTTAGACATTCTAAAGGAATGTGGTGCAACGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAATGATGCCGTGGGGTCTCTGCTGGAGGATAAAGGCCTACCTGCAGGACTGTTGGAGGAGCTGCCAACCAGATGGGAGCGCCTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCCGTTATGGGACTCAATTGCTGAGGAGCTTTGGCCCGCTGTTGCCAAATCAGTAAATGCTGTTCGCCTTGCTCGCCAAGGCCGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGTTGG

Protein Analysis

747

Amino Acids

82.42

Weight (kDa)

6.56

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 14 - 133 1.5e-34 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 181 - 354 2e-41 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 193 - 307 4.9e-19 FBX42, beta-propeller domain
Kelch_HCF PF13854 193 - 355 1e-14 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 198 - 277 1.2e-07 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 224 - 325 6e-11 FKB95, Kelch-repeats domain
Beta-prop_TYW4 PF13418 233 - 468 3.7e-27 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_2 PF07646 235 - 276 3.6e-06 Kelch motif
Kelch_1 PF01344 235 - 275 2.1e-08 Kelch motif
Beta-prop_Calicin PF13964 246 - 377 7.6e-10 Calicin, beta-propeller domain
Beta-prop_FBX42 PF13415 247 - 422 1.1e-34 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 262 - 334 6.8e-11 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 286 - 327 3e-09 Kelch motif
Kelch_2 PF07646 286 - 326 2.3e-08 Kelch motif
Beta-prop_FBX42 PF13415 287 - 457 1.4e-26 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 290 - 461 2.9e-38 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 313 - 456 3.4e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 323 - 372 4.1e-07 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 337 - 373 4.6e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 363 - 463 3.1e-09 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 668 - 745 1e-14 TYW2 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 34
AatII GACGTC 1 cut(s) 38
Acc36I ACCTGC 2 cut(s) 160, 2023
AccB7I CCANNNNNTGG 2 cut(s) 1246, 1466
AccBSI CCGCTC 1 cut(s) 408
AccI GTMKAC 1 cut(s) 1516
AccII CGCG 3 cut(s) 248, 294, 312
AclI AACGTT 1 cut(s) 1234
AclWI GGATC 3 cut(s) 761, 895, 1045
AcsI RAATTY 5 cut(s) 350, 777, 1567, 1757, 1798
AcuI CTGAAG 1 cut(s) 1572
AcyI GRCGYC 2 cut(s) 35, 1142
AfaI GTAC 7 cut(s) 948, 1094, 1190, 1320, 1393, 2197, 2209
AfiI CCNNNNNNNGG 7 cut(s) 431, 456, 710, 1164, 1246, 1466, 1841
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 857
AluBI AGCT 6 cut(s) 88, 1045, 1556, 1918, 2035, 2125
AluI AGCT 6 cut(s) 88, 1045, 1556, 1918, 2035, 2125
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 3 cut(s) 312, 1080, 1995
AlwI GGATC 3 cut(s) 761, 895, 1045
AlwNI CAGNNNCTG 1 cut(s) 175
AoxI GGCC 5 cut(s) 357, 712, 2008, 2129, 2176
ApeKI GCWGC 4 cut(s) 255, 872, 875, 2035
ApoI RAATTY 5 cut(s) 350, 777, 1567, 1757, 1798
AspLEI GCGC 2 cut(s) 169, 2055
AspS9I GGNCC 5 cut(s) 372, 712, 796, 998, 2130
AsuC2I CCSGG 3 cut(s) 199, 457, 514
AsuHPI GGTGA 6 cut(s) 577, 693, 854, 1082, 1355, 1861
AsuII TTCGAA 1 cut(s) 227
AvaII GGWCC 3 cut(s) 372, 796, 998
BaeGI GKGCMC 1 cut(s) 1009
BanII GRGCYC 1 cut(s) 90
BarI GAAGNNNNNNTAC 2 cut(s) 1713, 1745
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 2118
BbvI GCAGC 4 cut(s) 242, 859, 887, 2022
BccI CCATC 3 cut(s) 1061, 1589, 2040
BceAI ACGGC 5 cut(s) 125, 735, 863, 1318, 1967
BciT130I CCWGG 1 cut(s) 859
BciVI GTATCC 1 cut(s) 946
BclI TGATCA 1 cut(s) 1819
BcnI CCSGG 3 cut(s) 199, 457, 514
BcoDI GTCTC 3 cut(s) 312, 1080, 1995
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 3 cut(s) 873, 965, 2016
BfoI RGCGCY 1 cut(s) 2056
BfuAI ACCTGC 2 cut(s) 160, 2023
BfuI GTATCC 1 cut(s) 946
BglII AGATCT 1 cut(s) 1693
BisI GCNGC 9 cut(s) 24, 71, 128, 162, 165, 256, 873, 876, 2036
BlsI GCNGC 9 cut(s) 25, 72, 129, 163, 166, 257, 874, 877, 2037
BmcAI AGTACT 1 cut(s) 2209
Bme1390I CCNGG 4 cut(s) 199, 457, 514, 859
Bme18I GGWCC 3 cut(s) 372, 796, 998
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 5 cut(s) 372, 712, 796, 998, 2130
BmiI GGNNCC 4 cut(s) 276, 326, 423, 797
BmrFI CCNGG 4 cut(s) 199, 457, 514, 859
BpiI GAAGAC 1 cut(s) 625
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 2 cut(s) 407, 2018
Bpu10I CCTNAGC 1 cut(s) 2118
Bpu14I TTCGAA 1 cut(s) 227
BpuMI CCSGG 3 cut(s) 199, 457, 514
BsaAI YACGTR 1 cut(s) 1322
BsaBI GATNNNNATC 1 cut(s) 1449
BsaHI GRCGYC 2 cut(s) 35, 1142
BsaI GGTCTC 1 cut(s) 1995
BsaJI CCNNGG 9 cut(s) 375, 513, 857, 879, 1587, 1716, 1983, 2056, 2173
BsaWI WCCGGW 1 cut(s) 363
BsaXI ACNNNNNCTCC 6 cut(s) 726, 756, 1540, 1570, 2052, 2082
Bsc4I CCNNNNNNNGG 7 cut(s) 431, 456, 710, 1164, 1246, 1466, 1841
Bse1I ACTGG 5 cut(s) 954, 1223, 1296, 2078, 2197
Bse8I GATNNNNATC 1 cut(s) 1449
BseBI CCWGG 1 cut(s) 859
BseDI CCNNGG 9 cut(s) 375, 513, 857, 879, 1587, 1716, 1983, 2056, 2173
BseGI GGATG 3 cut(s) 482, 1519, 1621
BseJI GATNNNNATC 1 cut(s) 1449
BseLI CCNNNNNNNGG 7 cut(s) 431, 456, 710, 1164, 1246, 1466, 1841
BseMII CTCAG 6 cut(s) 32, 762, 773, 1055, 1430, 2109
BseNI ACTGG 5 cut(s) 954, 1223, 1296, 2078, 2197
BseRI GAGGAG 3 cut(s) 539, 2045, 2135
BseSI GKGCMC 1 cut(s) 1009
BseXI GCAGC 4 cut(s) 242, 859, 887, 2022
BseYI CCCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 169
Bsh1236I CGCG 3 cut(s) 248, 294, 312
BshFI GGCC 5 cut(s) 359, 714, 2010, 2131, 2178
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 8 cut(s) 67, 83, 198, 213, 364, 425, 456, 513
BslFI GGGAC 4 cut(s) 167, 1140, 2120, 2216
BslI CCNNNNNNNGG 7 cut(s) 431, 456, 710, 1164, 1246, 1466, 1841
BsmAI GTCTC 3 cut(s) 312, 1080, 1995
BsmFI GGGAC 4 cut(s) 167, 1140, 2120, 2216
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 5 cut(s) 359, 714, 2010, 2131, 2178
Bso31I GGTCTC 1 cut(s) 1995
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 2 cut(s) 90, 1009
Bsp1407I TGTACA 2 cut(s) 1092, 1188
Bsp143I GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
Bsp19I CCATGG 1 cut(s) 1587
Bsp68I TCGCGA 3 cut(s) 248, 294, 312
BspANI GGCC 5 cut(s) 359, 714, 2010, 2131, 2178
BspCNI CTCAG 6 cut(s) 33, 763, 774, 1054, 1431, 2110
BspFNI CGCG 3 cut(s) 248, 294, 312
BspLI GGNNCC 4 cut(s) 276, 326, 423, 797
BspMAI CTGCAG 3 cut(s) 877, 969, 2020
BspMI ACCTGC 2 cut(s) 160, 2023
BspPI GGATC 3 cut(s) 761, 895, 1045
BspQI GCTCTTC 1 cut(s) 1496
BspT104I TTCGAA 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 1995
BsrBI CCGCTC 1 cut(s) 408
BsrGI TGTACA 2 cut(s) 1092, 1188
BsrI ACTGG 5 cut(s) 954, 1223, 1296, 2078, 2197
BssECI CCNNGG 9 cut(s) 375, 513, 857, 879, 1587, 1716, 1983, 2056, 2173
BssMI GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
BssNI GRCGYC 2 cut(s) 35, 1142
BssT1I CCWWGG 3 cut(s) 1587, 2056, 2173
Bst2UI CCWGG 1 cut(s) 859
Bst4CI ACNGT 3 cut(s) 1269, 2025, 2207
Bst6I CTCTTC 3 cut(s) 362, 457, 1496
BstACI GRCGYC 2 cut(s) 35, 1142
BstAUI TGTACA 2 cut(s) 1092, 1188
BstBAI YACGTR 1 cut(s) 1322
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 3 cut(s) 556, 2133, 2170
BstDEI CTNAG 7 cut(s) 41, 771, 782, 1041, 1439, 1885, 2118
BstDSI CCRYGG 4 cut(s) 879, 1587, 1716, 1983
BstEII GGTNACC 1 cut(s) 1867
BstF5I GGATG 3 cut(s) 482, 1519, 1621
BstFNI CGCG 3 cut(s) 248, 294, 312
BstH2I RGCGCY 1 cut(s) 2056
BstHHI GCGC 2 cut(s) 169, 2055
BstKTI GATC 7 cut(s) 47, 769, 890, 1040, 1351, 1696, 1822
BstMAI GTCTC 3 cut(s) 312, 1080, 1995
BstMBI GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
BstMWI GCNNNNNNNGC 2 cut(s) 79, 2169
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 5 cut(s) 626, 872, 947, 1276, 1286
BstPI GGTNACC 1 cut(s) 1867
BstSCI CCNGG 4 cut(s) 197, 455, 512, 857
BstSFI CTRYAG 3 cut(s) 873, 965, 2016
BstSLI GKGCMC 1 cut(s) 1009
BstSNI TACGTA 1 cut(s) 1322
BstUI CGCG 3 cut(s) 248, 294, 312
BstV1I GCAGC 4 cut(s) 242, 859, 887, 2022
BstV2I GAAGAC 1 cut(s) 625
BstX2I RGATCY 2 cut(s) 1037, 1693
BstXI CCANNNNNNTGG 1 cut(s) 2046
BstYI RGATCY 2 cut(s) 1037, 1693
BsuI GTATCC 1 cut(s) 946
BsuRI GGCC 5 cut(s) 359, 714, 2010, 2131, 2178
BtgI CCRYGG 4 cut(s) 879, 1587, 1716, 1983
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 3 cut(s) 482, 1519, 1621
BtsI GCAGTG 2 cut(s) 974, 1326
BtsIMutI CAGTG 6 cut(s) 38, 644, 974, 994, 1326, 2085
BtuMI TCGCGA 3 cut(s) 248, 294, 312
BveI ACCTGC 2 cut(s) 160, 2023
Cac8I GCNNGC 3 cut(s) 556, 2133, 2170
CaiI CAGNNNCTG 1 cut(s) 175
CfoI GCGC 2 cut(s) 169, 2055
Cfr13I GGNCC 5 cut(s) 372, 712, 796, 998, 2130
CseI GACGC 3 cut(s) 37, 183, 1131
Csp6I GTAC 7 cut(s) 947, 1093, 1189, 1319, 1392, 2196, 2208
CviAII CATG 8 cut(s) 623, 869, 944, 1160, 1180, 1273, 1283, 1588
CviQI GTAC 7 cut(s) 947, 1093, 1189, 1319, 1392, 2196, 2208
DdeI CTNAG 7 cut(s) 41, 771, 782, 1041, 1439, 1885, 2118
DpnI GATC 7 cut(s) 46, 768, 889, 1039, 1350, 1695, 1821
DpnII GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
Eam1104I CTCTTC 3 cut(s) 362, 457, 1496
EarI CTCTTC 3 cut(s) 362, 457, 1496
EciI GGCGGA 2 cut(s) 88, 924
Ecl136II GAGCTC 1 cut(s) 88
Eco105I TACGTA 1 cut(s) 1322
Eco130I CCWWGG 3 cut(s) 1587, 2056, 2173
Eco147I AGGCCT 1 cut(s) 2010
Eco24I GRGCYC 1 cut(s) 90
Eco31I GGTCTC 1 cut(s) 1995
Eco47I GGWCC 3 cut(s) 372, 796, 998
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 1572
Eco91I GGTNACC 1 cut(s) 1867
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 796
EcoO65I GGTNACC 1 cut(s) 1867
EcoRII CCWGG 1 cut(s) 857
EcoT14I CCWWGG 3 cut(s) 1587, 2056, 2173
EcoT22I ATGCAT 2 cut(s) 1387, 1621
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 3 cut(s) 1587, 2056, 2173
FaeI CATG 8 cut(s) 626, 872, 947, 1163, 1183, 1276, 1286, 1591
FaqI GGGAC 4 cut(s) 167, 1140, 2120, 2216
FatI CATG 8 cut(s) 622, 868, 943, 1159, 1179, 1272, 1282, 1587
FauI CCCGC 2 cut(s) 1361, 2140
FauNDI CATATG 1 cut(s) 1791
FbaI TGATCA 1 cut(s) 1819
FblI GTMKAC 1 cut(s) 1516
Fnu4HI GCNGC 9 cut(s) 24, 71, 128, 162, 165, 256, 873, 876, 2036
FokI GGATG 3 cut(s) 489, 1506, 1628
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 9 cut(s) 24, 71, 128, 162, 165, 256, 873, 876, 2036
FspBI CTAG 1 cut(s) 483
GlaI GCGC 2 cut(s) 168, 2054
GluI GCNGC 9 cut(s) 24, 71, 128, 162, 165, 256, 873, 876, 2036
GsaI CCCAGC 1 cut(s) 284
GsuI CTGGAG 2 cut(s) 407, 2018
HaeII RGCGCY 1 cut(s) 2056
HaeIII GGCC 5 cut(s) 359, 714, 2010, 2131, 2178
HapII CCGG 8 cut(s) 67, 83, 198, 213, 364, 425, 456, 513
HgaI GACGC 3 cut(s) 37, 183, 1131
HhaI GCGC 2 cut(s) 169, 2055
Hin1I GRCGYC 2 cut(s) 35, 1142
Hin1II CATG 8 cut(s) 626, 872, 947, 1163, 1183, 1276, 1286, 1591
Hin6I GCGC 2 cut(s) 167, 2053
HinP1I GCGC 2 cut(s) 167, 2053
HincII GTYRAC 1 cut(s) 1607
HindII GTYRAC 1 cut(s) 1607
HinfI GANTC 9 cut(s) 120, 209, 250, 529, 586, 1295, 2095, 2108, 2218
HpaII CCGG 8 cut(s) 67, 83, 198, 213, 364, 425, 456, 513
HphI GGTGA 6 cut(s) 577, 693, 854, 1082, 1355, 1861
Hpy166II GTNNAC 6 cut(s) 1189, 1457, 1517, 1607, 1711, 1730
Hpy188I TCNGA 9 cut(s) 49, 119, 206, 501, 783, 1434, 1632, 1765, 1943
Hpy188III TCNNGA 9 cut(s) 10, 247, 293, 311, 386, 770, 1035, 1559, 2092
Hpy8I GTNNAC 6 cut(s) 1189, 1457, 1517, 1607, 1711, 1730
Hpy99I CGWCG 2 cut(s) 31, 146
HpyCH4III ACNGT 3 cut(s) 1269, 2025, 2207
HpyCH4IV ACGT 4 cut(s) 35, 156, 1234, 1321
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 2169
HpyF3I CTNAG 7 cut(s) 41, 771, 782, 1041, 1439, 1885, 2118
HpySE526I ACGT 4 cut(s) 35, 156, 1234, 1321
Hsp92I GRCGYC 2 cut(s) 35, 1142
Hsp92II CATG 8 cut(s) 626, 872, 947, 1163, 1183, 1276, 1286, 1591
HspAI GCGC 2 cut(s) 167, 2053
Ksp22I TGATCA 1 cut(s) 1819
Kzo9I GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
LguI GCTCTTC 1 cut(s) 1496
LmnI GCTCC 8 cut(s) 49, 85, 93, 427, 1561, 2032, 2050, 2122
Lsp1109I GCAGC 4 cut(s) 242, 859, 887, 2022
MaeI CTAG 1 cut(s) 483
MaeII ACGT 4 cut(s) 35, 156, 1234, 1321
MaeIII GTNAC 4 cut(s) 36, 928, 1867, 2080
MalI GATC 7 cut(s) 46, 768, 889, 1039, 1350, 1695, 1821
MbiI CCGCTC 1 cut(s) 408
MboI GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
MfeI CAATTG 2 cut(s) 1080, 2112
MflI RGATCY 2 cut(s) 1037, 1693
MhlI GDGCHC 2 cut(s) 90, 1009
MlyI GAGTC 2 cut(s) 218, 2102
MmeI TCCRAC 4 cut(s) 712, 1323, 1447, 2007
Mph1103I ATGCAT 2 cut(s) 1387, 1621
MseI TTAA 4 cut(s) 915, 1539, 1547, 1572
MslI CAYNNNNRTG 2 cut(s) 867, 1184
MspA1I CMGCKG 3 cut(s) 130, 280, 2135
MspI CCGG 8 cut(s) 67, 83, 198, 213, 364, 425, 456, 513
MspR9I CCNGG 4 cut(s) 199, 457, 514, 859
MunI CAATTG 2 cut(s) 1080, 2112
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 3 cut(s) 248, 294, 312
MwoI GCNNNNNNNGC 2 cut(s) 79, 2169
NciI CCSGG 3 cut(s) 199, 457, 514
NcoI CCATGG 1 cut(s) 1587
NdeI CATATG 1 cut(s) 1791
NdeII GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
NlaIII CATG 8 cut(s) 626, 872, 947, 1163, 1183, 1276, 1286, 1591
NlaIV GGNNCC 4 cut(s) 276, 326, 423, 797
NmeAIII GCCGAG 1 cut(s) 2204
NmuCI GTSAC 4 cut(s) 36, 928, 1867, 2080
NruI TCGCGA 3 cut(s) 248, 294, 312
NsiI ATGCAT 2 cut(s) 1387, 1621
NspI RCATGY 5 cut(s) 626, 872, 947, 1276, 1286
NspV TTCGAA 1 cut(s) 227
PceI AGGCCT 1 cut(s) 2010
PciSI GCTCTTC 1 cut(s) 1496
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 351, 2176
PctI GAATGC 1 cut(s) 451
PfeI GAWTC 7 cut(s) 120, 250, 529, 586, 1295, 2095, 2218
PflMI CCANNNNNTGG 2 cut(s) 1246, 1466
PkrI GCNGC 9 cut(s) 25, 72, 129, 163, 166, 257, 874, 877, 2037
PleI GAGTC 2 cut(s) 217, 2102
PpsI GAGTC 2 cut(s) 217, 2102
Ppu21I YACGTR 1 cut(s) 1322
PpuMI RGGWCCY 1 cut(s) 796
Psp124BI GAGCTC 1 cut(s) 90
Psp1406I AACGTT 1 cut(s) 1234
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 1 cut(s) 857
PspEI GGTNACC 1 cut(s) 1867
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 1 cut(s) 857
PspN4I GGNNCC 4 cut(s) 276, 326, 423, 797
PspPI GGNCC 5 cut(s) 372, 712, 796, 998, 2130
PspPPI RGGWCCY 1 cut(s) 796
PsrI GAACNNNNNNTAC 4 cut(s) 308, 340, 2142, 2174
PstI CTGCAG 3 cut(s) 877, 969, 2020
PstNI CAGNNNCTG 1 cut(s) 175
PsuI RGATCY 2 cut(s) 1037, 1693
RruI TCGCGA 3 cut(s) 248, 294, 312
RsaI GTAC 7 cut(s) 948, 1094, 1190, 1320, 1393, 2197, 2209
RsaNI GTAC 7 cut(s) 947, 1093, 1189, 1319, 1392, 2196, 2208
RseI CAYNNNNRTG 2 cut(s) 867, 1184
SacI GAGCTC 1 cut(s) 90
SapI GCTCTTC 1 cut(s) 1496
SaqAI TTAA 4 cut(s) 915, 1539, 1547, 1572
SatI GCNGC 9 cut(s) 24, 71, 128, 162, 165, 256, 873, 876, 2036
Sau3AI GATC 7 cut(s) 44, 766, 887, 1037, 1348, 1693, 1819
Sau96I GGNCC 5 cut(s) 372, 712, 796, 998, 2130
SbfI CCTGCAGG 1 cut(s) 2020
ScaI AGTACT 1 cut(s) 2209
SchI GAGTC 2 cut(s) 218, 2102
ScrFI CCNGG 4 cut(s) 199, 457, 514, 859
SdaI CCTGCAGG 1 cut(s) 2020
SduI GDGCHC 2 cut(s) 90, 1009
SfcI CTRYAG 3 cut(s) 873, 965, 2016
SfuI TTCGAA 1 cut(s) 227
SinI GGWCC 3 cut(s) 372, 796, 998
SmiMI CAYNNNNRTG 2 cut(s) 867, 1184
SnaBI TACGTA 1 cut(s) 1322
Sse8387I CCTGCAGG 1 cut(s) 2020
SseBI AGGCCT 1 cut(s) 2010
SspMI CTAG 1 cut(s) 483
SstI GAGCTC 1 cut(s) 90
StuI AGGCCT 1 cut(s) 2010
StyD4I CCNGG 4 cut(s) 197, 455, 512, 857
StyI CCWWGG 3 cut(s) 1587, 2056, 2173
TaaI ACNGT 3 cut(s) 1269, 2025, 2207
TaiI ACGT 4 cut(s) 38, 159, 1237, 1324
TaqII GACCGA 1 cut(s) 389
TatI WGTACW 4 cut(s) 946, 1092, 1188, 2207
TauI GCSGC 5 cut(s) 26, 73, 130, 164, 167
TfiI GAWTC 7 cut(s) 120, 250, 529, 586, 1295, 2095, 2218
Tru1I TTAA 4 cut(s) 915, 1539, 1547, 1572
Tru9I TTAA 4 cut(s) 915, 1539, 1547, 1572
TscAI CASTG 6 cut(s) 45, 644, 974, 994, 1333, 2085
TseFI GTSAC 4 cut(s) 36, 928, 1867, 2080
TseI GCWGC 4 cut(s) 255, 872, 875, 2035
Tsp45I GTSAC 4 cut(s) 36, 928, 1867, 2080
TspDTI ATGAA 3 cut(s) 1427, 1791, 1988
TspGWI ACGGA 3 cut(s) 990, 1705, 2088
TspRI CASTG 6 cut(s) 45, 644, 974, 994, 1333, 2085
Van91I CCANNNNNTGG 2 cut(s) 1246, 1466
VpaK11BI GGWCC 3 cut(s) 372, 796, 998
XapI RAATTY 5 cut(s) 350, 777, 1567, 1757, 1798
XceI RCATGY 5 cut(s) 626, 872, 947, 1276, 1286
XmiI GTMKAC 1 cut(s) 1516
XspI CTAG 1 cut(s) 483
ZraI GACGTC 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 2209
Zsp2I ATGCAT 2 cut(s) 1387, 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.