RchiOBHm_Chr5g0003171

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006279
Physical Location & Seq
Forward (+)
1929051 .. 1934107
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28449

Sequence Viewer

Length: 3063 bp
ATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCACTGAGATCGGAGGTGACGGACAAGTCGCCGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACGTCATCAACGGCCACCGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGCGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGACCTCGTCTTCTGCTCCTCCGAACAGAATGAGCAGGAGCTCCAAGACGACCAAAACGACGTTGTTTTCAGATTCGAGCCACTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCGCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGATGCTTTTCTGGAGGCTCTGCAGAGTGGGGAGAGCGGAGGAGGGTTTGCGGTGGCTCCTGCTGGTGGAAATTACGAGAATGCCCCTTCCGGGTTGAAGGGAGATAGTGGATGCTCTAGTCTGTCTGTTGTTCCGATAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCGGCTTGCTCATTGGTGAAGAAGGCGGATAATGGGATTCTTGTGTTTGGTGGATTTGGAGGCATTGGAAGACATGCTAGGAGAAATCAGTGTTTGCTGGTTGATACATTGTCTGGTAGTTTGAGAGGGATTAGCATAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGAGATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCACCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAGGATCAAAGATATATGTGTTTGGTGGACTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGATACAGATAATCTGCAGTGGAGAGAGATAGTTGTCAGTGAGGAAGGTCCGTGTGCCCGACATTCACACTCAATGGTGGCATCGGGATCTCAGCTATATATGTTTGGAGGATATGATGGTGAGGAAGCAATTGGAGACTTGTACAGGTTTGATACTGAAAAATCTAAATGTAAATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCACACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACGTTGCCAAGAGTTGGCAATACTTGATTTACGACTGTGCATGTGGAGGCATGTGAAACTGGAATCCAACGGCGAAGATTTGTTTGTACGTAGCACTGCCAATGTTTTTGGTGATGATCTTGTTATGATTGGTGGCGGGGCATCTTGCTATGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCTCTGATGCTGAGAGATGATAATCGTAAACCACTCGTTGCAGAACTGCATACTGACCAAATTGATATTGTGAAGAGCGAAAAGAGTAGACATCCACAGGTTCAAAATGTTAAAACATTAACCGAAGCTCCTGACCTAAATTTTAAGAGTGAATTACCCATGGCTGATGGCATTGGTCAACAAGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGGAAGGACATACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCGTGGAAGGTGGTTTACATATTTGTTTCCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCAGCATCATATGGCAAATTCATTTGAAGGACAGAGTGATCACATTTGTAAGCCAGATATAGGAAAGGAATGTTCGATTGATGAGGTCACCTGTTCAACAGCCTTAGACATTCTAAAGGAATGTGGTGCAACGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAGTGATGCCGTGGGGTCTCTGCTGGAGGATAAAGGCCTACCTGCAGGACTGTTGGAGGAGCTGCCAACCAGATGGGAGCGCCTTGGAGATATTGTTGTGCTTCCAGTGACATCATTCAAGAATCCGTTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGCTGTTGCCAAATCAGTAAATGCTGTTCGCCTTGCTCGCCAAGGCCGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGTTGGGTTGATCATCGTGAAAATGGAATTCTCTATTCTTTTGATGCTACTAAGTGCATGTTCTCCTGGGGCAACCTTTCTGAGAAGCGCCGCATGGGCTCTCTGAATTGTAGAGATGAGATCATTGTGGATTTATTTGCTGGAATTGGATATTTTGTGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGCGGCACTCCGGAGAAACGTACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGGACAACGGCACCTACAGGAGTGGCTGATCGGGTCTGCCTTGGTCTCATTCCATCAAGTGAATCTAGCTGGGCCACTGCTGTTAGGGCATTAAGGGGTGAGGGTGGGATGCTACATGTGCATGGGAATGTGGTGGACTCAGAGGAGAATTCATGGACCAAGCACGTAACAGAATCAGTAGCTGAAACTGCTAGATCTCAAGGTCATTGTTGGGAGGTTTCAATAGAACATTTGGAGAGAGTTAAATGGTACGCCCCACACATCCGCCACGTTGTTGCAGATGTAAGATGTAGACAGACCCAGAAATAA

Protein Analysis

1020

Amino Acids

112.33

Weight (kDa)

6.76

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 205 2.8e-67 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 256 - 429 9e-41 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 268 - 399 3.8e-21 FBX42, beta-propeller domain
Kelch_HCF PF13854 269 - 430 3.2e-14 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 273 - 352 2.2e-07 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 296 - 403 9.8e-11 FKB95, Kelch-repeats domain
Kelch_HCF PF13854 307 - 531 1.4e-19 Host cell factor, Kelch-repeats domain
Beta-prop_TYW4 PF13418 308 - 543 2e-27 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 310 - 350 4.4e-08 Kelch motif
Kelch_2 PF07646 310 - 353 3e-06 Kelch motif
Beta-prop_Calicin PF13964 321 - 452 6.9e-10 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 337 - 406 1.6e-10 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 361 - 401 3.4e-08 Kelch motif
Kelch_1 PF01344 361 - 402 4.3e-09 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 365 - 536 1.1e-38 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 382 - 497 2.9e-20 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 398 - 447 2.5e-07 Attractin/LZTR1 beta-propeller
Kelch_2 PF07646 412 - 448 2.4e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 424 - 538 1e-12 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 743 - 820 1.5e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 825 - 975 4.1e-32 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 34, 61
AatII GACGTC 2 cut(s) 38, 111
Acc36I ACCTGC 2 cut(s) 376, 2248
AccB1I GGYRCC 1 cut(s) 2764
AccB7I CCANNNNNTGG 1 cut(s) 1471
AccBSI CCGCTC 2 cut(s) 182, 627
AccI GTMKAC 2 cut(s) 1741, 3046
AccII CGCG 5 cut(s) 126, 218, 464, 510, 528
AccIII TCCGGA 1 cut(s) 2694
AclI AACGTT 1 cut(s) 1459
AclWI GGATC 4 cut(s) 242, 986, 1120, 1270
AcoI YGGCCR 3 cut(s) 118, 154, 243
AcsI RAATTY 7 cut(s) 566, 1002, 1792, 1982, 2023, 2487, 2902
AcyI GRCGYC 3 cut(s) 35, 108, 1367
AfaI GTAC 9 cut(s) 1173, 1319, 1415, 1545, 1618, 2422, 2434, 2706, 3005
AfiI CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2066, 2761
AflIII ACRYGT 1 cut(s) 2869
AhlI ACTAGT 1 cut(s) 2650
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 2 cut(s) 1082, 2525
AluBI AGCT 9 cut(s) 144, 304, 1270, 1781, 2143, 2260, 2350, 2823, 2936
AluI AGCT 9 cut(s) 144, 304, 1270, 1781, 2143, 2260, 2350, 2823, 2936
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 5 cut(s) 196, 528, 1305, 2220, 2804
AlwI GGATC 4 cut(s) 242, 986, 1120, 1270
AlwNI CAGNNNCTG 3 cut(s) 147, 391, 2936
Aor13HI TCCGGA 1 cut(s) 2694
ApeKI GCWGC 5 cut(s) 471, 1097, 1100, 2260, 2620
ApoI RAATTY 7 cut(s) 566, 1002, 1792, 1982, 2023, 2487, 2902
AspLEI GCGC 4 cut(s) 218, 385, 2280, 2550
AspS9I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2637, 2826, 2910
AsuC2I CCSGG 5 cut(s) 188, 242, 415, 682, 739
AsuII TTCGAA 1 cut(s) 443
AvaII GGWCC 4 cut(s) 588, 1021, 1223, 2910
BaeGI GKGCMC 2 cut(s) 77, 1234
BanI GGYRCC 1 cut(s) 2764
BanII GRGCYC 2 cut(s) 306, 2561
BarI GAAGNNNNNNTAC 2 cut(s) 1938, 1970
BbsI GAAGAC 2 cut(s) 265, 850
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 2343
BbvI GCAGC 5 cut(s) 458, 1084, 1112, 2247, 2607
BccI CCATC 5 cut(s) 173, 1286, 1814, 2265, 2815
BceAI ACGGC 7 cut(s) 133, 341, 960, 1088, 1543, 2192, 2778
BciT130I CCWGG 2 cut(s) 1084, 2527
BciVI GTATCC 1 cut(s) 1171
BclI TGATCA 2 cut(s) 2044, 2470
BcnI CCSGG 5 cut(s) 188, 242, 415, 682, 739
BcoDI GTCTC 5 cut(s) 196, 528, 1305, 2220, 2804
BcuI ACTAGT 1 cut(s) 2650
BfaI CTAG 5 cut(s) 708, 852, 2651, 2820, 2946
BfmI CTRYAG 5 cut(s) 611, 1098, 1190, 2241, 2769
BfoI RGCGCY 2 cut(s) 2281, 2551
BfuAI ACCTGC 2 cut(s) 376, 2248
BfuI GTATCC 1 cut(s) 1171
BglI GCCNNNNNGGC 1 cut(s) 2556
BglII AGATCT 2 cut(s) 1918, 2948
BmcAI AGTACT 1 cut(s) 2434
Bme1390I CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2527
Bme18I GGWCC 4 cut(s) 588, 1021, 1223, 2910
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2637, 2826, 2910
BmiI GGNNCC 5 cut(s) 492, 542, 648, 1022, 2766
BmrFI CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2527
BpiI GAAGAC 2 cut(s) 265, 850
BpmI CTGGAG 2 cut(s) 623, 2243
Bpu10I CCTNAGC 1 cut(s) 2343
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 2937
BpuMI CCSGG 5 cut(s) 188, 242, 415, 682, 739
Bsa29I ATCGAT 1 cut(s) 2728
BsaAI YACGTR 2 cut(s) 1547, 2920
BsaBI GATNNNNATC 1 cut(s) 1674
BsaHI GRCGYC 3 cut(s) 35, 108, 1367
BsaI GGTCTC 3 cut(s) 196, 2220, 2804
BsaWI WCCGGW 3 cut(s) 579, 2694, 2754
BsaXI ACNNNNNCTCC 6 cut(s) 951, 981, 1765, 1795, 2277, 2307
Bsc4I CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2066, 2761
Bse1I ACTGG 5 cut(s) 1179, 1448, 1521, 2303, 2422
Bse8I GATNNNNATC 1 cut(s) 1674
BseAI TCCGGA 1 cut(s) 2694
BseBI CCWGG 2 cut(s) 1084, 2527
BseCI ATCGAT 1 cut(s) 2728
BseGI GGATG 6 cut(s) 165, 707, 1744, 1846, 2868, 3015
BseJI GATNNNNATC 1 cut(s) 1674
BseLI CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2066, 2761
BseMII CTCAG 9 cut(s) 32, 188, 987, 998, 1280, 1655, 2334, 2532, 2907
BseNI ACTGG 5 cut(s) 1179, 1448, 1521, 2303, 2422
BseRI GAGGAG 7 cut(s) 92, 271, 645, 764, 2270, 2360, 2912
BseSI GKGCMC 2 cut(s) 77, 1234
BseX3I CGGCCG 2 cut(s) 154, 243
BseXI GCAGC 5 cut(s) 458, 1084, 1112, 2247, 2607
BseYI CCCAGC 2 cut(s) 496, 2823
BsgI GTGCAG 1 cut(s) 385
Bsh1236I CGCG 5 cut(s) 126, 218, 464, 510, 528
Bsh1285I CGRYCG 2 cut(s) 157, 246
BshNI GGYRCC 1 cut(s) 2764
BshVI ATCGAT 1 cut(s) 2728
BsiEI CGRYCG 2 cut(s) 157, 246
BsiHKAI GWGCWC 1 cut(s) 306
BslFI GGGAC 4 cut(s) 383, 1365, 2345, 2441
BslI CCNNNNNNNGG 8 cut(s) 186, 656, 681, 935, 1389, 1471, 2066, 2761
BsmAI GTCTC 5 cut(s) 196, 528, 1305, 2220, 2804
BsmFI GGGAC 4 cut(s) 383, 1365, 2345, 2441
BsmI GAATGC 1 cut(s) 676
Bso31I GGTCTC 3 cut(s) 196, 2220, 2804
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 4 cut(s) 77, 306, 1234, 2561
Bsp13I TCCGGA 1 cut(s) 2694
Bsp1407I TGTACA 2 cut(s) 1317, 1413
Bsp19I CCATGG 1 cut(s) 1812
Bsp68I TCGCGA 3 cut(s) 464, 510, 528
BspCNI CTCAG 9 cut(s) 33, 187, 988, 999, 1279, 1656, 2335, 2533, 2906
BspDI ATCGAT 1 cut(s) 2728
BspEI TCCGGA 1 cut(s) 2694
BspFNI CGCG 5 cut(s) 126, 218, 464, 510, 528
BspLI GGNNCC 5 cut(s) 492, 542, 648, 1022, 2766
BspMAI CTGCAG 4 cut(s) 615, 1102, 1194, 2245
BspMI ACCTGC 2 cut(s) 376, 2248
BspPI GGATC 4 cut(s) 242, 986, 1120, 1270
BspQI GCTCTTC 1 cut(s) 1721
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 2764
BspTNI GGTCTC 3 cut(s) 196, 2220, 2804
BsrBI CCGCTC 2 cut(s) 182, 627
BsrGI TGTACA 2 cut(s) 1317, 1413
BsrI ACTGG 5 cut(s) 1179, 1448, 1521, 2303, 2422
BssNI GRCGYC 3 cut(s) 35, 108, 1367
BssT1I CCWWGG 5 cut(s) 1812, 2281, 2398, 2640, 2794
Bst2UI CCWGG 2 cut(s) 1084, 2527
Bst4CI ACNGT 4 cut(s) 79, 1494, 2250, 2432
Bst6I CTCTTC 2 cut(s) 578, 1721
BstACI GRCGYC 3 cut(s) 35, 108, 1367
BstAUI TGTACA 2 cut(s) 1317, 1413
BstBAI YACGTR 2 cut(s) 1547, 2920
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 5 cut(s) 180, 184, 781, 2358, 2395
BstDSI CCRYGG 4 cut(s) 1104, 1812, 1941, 2208
BstEII GGTNACC 1 cut(s) 2092
BstF5I GGATG 6 cut(s) 165, 707, 1744, 1846, 2868, 3015
BstFNI CGCG 5 cut(s) 126, 218, 464, 510, 528
BstH2I RGCGCY 2 cut(s) 2281, 2551
BstHHI GCGC 4 cut(s) 218, 385, 2280, 2550
BstMAI GTCTC 5 cut(s) 196, 528, 1305, 2220, 2804
BstMCI CGRYCG 2 cut(s) 157, 246
BstMWI GCNNNNNNNGC 6 cut(s) 2394, 2556, 2687, 2840, 2872, 2942
BstNI CCWGG 2 cut(s) 1084, 2527
BstNSI RCATGY 7 cut(s) 851, 1097, 1172, 1501, 1511, 2521, 2873
BstPI GGTNACC 1 cut(s) 2092
BstSCI CCNGG 7 cut(s) 186, 240, 413, 680, 737, 1082, 2525
BstSFI CTRYAG 5 cut(s) 611, 1098, 1190, 2241, 2769
BstSLI GKGCMC 2 cut(s) 77, 1234
BstSNI TACGTA 1 cut(s) 1547
BstUI CGCG 5 cut(s) 126, 218, 464, 510, 528
BstV1I GCAGC 5 cut(s) 458, 1084, 1112, 2247, 2607
BstV2I GAAGAC 2 cut(s) 265, 850
BstX2I RGATCY 3 cut(s) 1262, 1918, 2948
BstXI CCANNNNNNTGG 1 cut(s) 2271
BstYI RGATCY 3 cut(s) 1262, 1918, 2948
BstZI CGGCCG 2 cut(s) 154, 243
Bsu15I ATCGAT 1 cut(s) 2728
BsuI GTATCC 1 cut(s) 1171
BsuTUI ATCGAT 1 cut(s) 2728
BtgI CCRYGG 4 cut(s) 1104, 1812, 1941, 2208
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 6 cut(s) 165, 707, 1744, 1846, 2868, 3015
BtsI GCAGTG 3 cut(s) 1199, 1551, 2829
BtsIMutI CAGTG 9 cut(s) 38, 344, 869, 1199, 1219, 1551, 2310, 2728, 2829
BtuMI TCGCGA 3 cut(s) 464, 510, 528
BveI ACCTGC 2 cut(s) 376, 2248
Cac8I GCNNGC 5 cut(s) 180, 184, 781, 2358, 2395
CaiI CAGNNNCTG 3 cut(s) 147, 391, 2936
CfoI GCGC 4 cut(s) 218, 385, 2280, 2550
Cfr13I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2637, 2826, 2910
ClaI ATCGAT 1 cut(s) 2728
CseI GACGC 3 cut(s) 37, 399, 1356
Csp6I GTAC 9 cut(s) 1172, 1318, 1414, 1544, 1617, 2421, 2433, 2705, 3004
CviQI GTAC 9 cut(s) 1172, 1318, 1414, 1544, 1617, 2421, 2433, 2705, 3004
DrdI GACNNNNNNGTC 2 cut(s) 34, 61
DseDI GACNNNNNNGTC 2 cut(s) 34, 61
EaeI YGGCCR 3 cut(s) 118, 154, 243
EagI CGGCCG 2 cut(s) 154, 243
Eam1104I CTCTTC 2 cut(s) 578, 1721
EarI CTCTTC 2 cut(s) 578, 1721
EciI GGCGGA 2 cut(s) 815, 3008
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 2 cut(s) 154, 243
Eco105I TACGTA 1 cut(s) 1547
Eco130I CCWWGG 5 cut(s) 1812, 2281, 2398, 2640, 2794
Eco147I AGGCCT 1 cut(s) 2235
Eco24I GRGCYC 2 cut(s) 306, 2561
Eco31I GGTCTC 3 cut(s) 196, 2220, 2804
Eco47I GGWCC 4 cut(s) 588, 1021, 1223, 2910
Eco52I CGGCCG 2 cut(s) 154, 243
Eco53kI GAGCTC 1 cut(s) 304
Eco91I GGTNACC 1 cut(s) 2092
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 1021
EcoO65I GGTNACC 1 cut(s) 2092
EcoRI GAATTC 2 cut(s) 2487, 2902
EcoRII CCWGG 2 cut(s) 1082, 2525
EcoT14I CCWWGG 5 cut(s) 1812, 2281, 2398, 2640, 2794
EcoT22I ATGCAT 3 cut(s) 1612, 1846, 2735
EcoT38I GRGCYC 2 cut(s) 306, 2561
ErhI CCWWGG 5 cut(s) 1812, 2281, 2398, 2640, 2794
FaqI GGGAC 4 cut(s) 383, 1365, 2345, 2441
FauI CCCGC 2 cut(s) 187, 1586
FauNDI CATATG 1 cut(s) 2016
FbaI TGATCA 2 cut(s) 2044, 2470
FblI GTMKAC 2 cut(s) 1741, 3046
FokI GGATG 6 cut(s) 152, 714, 1731, 1853, 2875, 3002
FriOI GRGCYC 2 cut(s) 306, 2561
FspBI CTAG 5 cut(s) 708, 852, 2651, 2820, 2946
GlaI GCGC 4 cut(s) 217, 384, 2279, 2549
GsaI CCCAGC 2 cut(s) 500, 2827
GsuI CTGGAG 2 cut(s) 623, 2243
HaeII RGCGCY 2 cut(s) 2281, 2551
HgaI GACGC 3 cut(s) 37, 399, 1356
HhaI GCGC 4 cut(s) 218, 385, 2280, 2550
Hin1I GRCGYC 3 cut(s) 35, 108, 1367
Hin6I GCGC 4 cut(s) 216, 383, 2278, 2548
HinP1I GCGC 4 cut(s) 216, 383, 2278, 2548
HincII GTYRAC 1 cut(s) 1832
HindII GTYRAC 1 cut(s) 1832
Hpy99I CGWCG 4 cut(s) 31, 110, 326, 362
HpyCH4III ACNGT 4 cut(s) 79, 1494, 2250, 2432
HpyCH4IV ACGT 9 cut(s) 35, 108, 324, 372, 1459, 1546, 2703, 2919, 3024
HpyF10VI GCNNNNNNNGC 6 cut(s) 2394, 2556, 2687, 2840, 2872, 2942
HpySE526I ACGT 9 cut(s) 35, 108, 324, 372, 1459, 1546, 2703, 2919, 3024
Hsp92I GRCGYC 3 cut(s) 35, 108, 1367
HspAI GCGC 4 cut(s) 216, 383, 2278, 2548
Kpn2I TCCGGA 1 cut(s) 2694
Ksp22I TGATCA 2 cut(s) 2044, 2470
LguI GCTCTTC 1 cut(s) 1721
Lsp1109I GCAGC 5 cut(s) 458, 1084, 1112, 2247, 2607
MaeI CTAG 5 cut(s) 708, 852, 2651, 2820, 2946
MaeII ACGT 9 cut(s) 35, 108, 324, 372, 1459, 1546, 2703, 2919, 3024
MaeIII GTNAC 6 cut(s) 36, 52, 1153, 2092, 2305, 2920
MbiI CCGCTC 2 cut(s) 182, 627
MboII GAAGA 9 cut(s) 211, 265, 595, 805, 855, 1048, 1152, 1544, 1738
MfeI CAATTG 2 cut(s) 1305, 2337
MflI RGATCY 3 cut(s) 1262, 1918, 2948
MhlI GDGCHC 4 cut(s) 77, 306, 1234, 2561
MlyI GAGTC 4 cut(s) 248, 434, 2327, 2885
MmeI TCCRAC 3 cut(s) 937, 1548, 2232
Mph1103I ATGCAT 3 cut(s) 1612, 1846, 2735
MroI TCCGGA 1 cut(s) 2694
MseI TTAA 6 cut(s) 1140, 1764, 1772, 1797, 2846, 2997
MslI CAYNNNNRTG 4 cut(s) 1092, 1409, 2874, 2880
MspA1I CMGCKG 1 cut(s) 496
MspR9I CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2527
MunI CAATTG 2 cut(s) 1305, 2337
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 2 cut(s) 1084, 2527
MvnI CGCG 5 cut(s) 126, 218, 464, 510, 528
MwoI GCNNNNNNNGC 6 cut(s) 2394, 2556, 2687, 2840, 2872, 2942
NciI CCSGG 5 cut(s) 188, 242, 415, 682, 739
NcoI CCATGG 1 cut(s) 1812
NdeI CATATG 1 cut(s) 2016
NlaIV GGNNCC 5 cut(s) 492, 542, 648, 1022, 2766
NmeAIII GCCGAG 1 cut(s) 2429
NmuCI GTSAC 5 cut(s) 36, 52, 1153, 2092, 2305
NruI TCGCGA 3 cut(s) 464, 510, 528
NsiI ATGCAT 3 cut(s) 1612, 1846, 2735
NspI RCATGY 7 cut(s) 851, 1097, 1172, 1501, 1511, 2521, 2873
NspV TTCGAA 1 cut(s) 443
PceI AGGCCT 1 cut(s) 2235
PciI ACATGT 1 cut(s) 2869
PciSI GCTCTTC 1 cut(s) 1721
PcsI WCGNNNNNNNCGW 5 cut(s) 123, 243, 318, 567, 2401
PctI GAATGC 1 cut(s) 676
PflFI GACNNNGTC 2 cut(s) 257, 269
PflMI CCANNNNNTGG 1 cut(s) 1471
PleI GAGTC 4 cut(s) 248, 433, 2327, 2885
PpsI GAGTC 4 cut(s) 248, 433, 2327, 2885
Ppu21I YACGTR 2 cut(s) 1547, 2920
PpuMI RGGWCCY 1 cut(s) 1021
PscI ACATGT 1 cut(s) 2869
Psp124BI GAGCTC 1 cut(s) 306
Psp1406I AACGTT 1 cut(s) 1459
Psp5II RGGWCCY 1 cut(s) 1021
Psp6I CCWGG 2 cut(s) 1082, 2525
PspEI GGTNACC 1 cut(s) 2092
PspFI CCCAGC 2 cut(s) 496, 2823
PspGI CCWGG 2 cut(s) 1082, 2525
PspN4I GGNNCC 5 cut(s) 492, 542, 648, 1022, 2766
PspPI GGNCC 7 cut(s) 588, 937, 1021, 1223, 2637, 2826, 2910
PspPPI RGGWCCY 1 cut(s) 1021
PsrI GAACNNNNNNTAC 4 cut(s) 524, 556, 2367, 2399
PstI CTGCAG 4 cut(s) 615, 1102, 1194, 2245
PstNI CAGNNNCTG 3 cut(s) 147, 391, 2936
PsuI RGATCY 3 cut(s) 1262, 1918, 2948
PsyI GACNNNGTC 2 cut(s) 257, 269
RruI TCGCGA 3 cut(s) 464, 510, 528
RsaI GTAC 9 cut(s) 1173, 1319, 1415, 1545, 1618, 2422, 2434, 2706, 3005
RsaNI GTAC 9 cut(s) 1172, 1318, 1414, 1544, 1617, 2421, 2433, 2705, 3004
RseI CAYNNNNRTG 4 cut(s) 1092, 1409, 2874, 2880
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 1721
SaqAI TTAA 6 cut(s) 1140, 1764, 1772, 1797, 2846, 2997
Sau96I GGNCC 7 cut(s) 588, 937, 1021, 1223, 2637, 2826, 2910
SbfI CCTGCAGG 1 cut(s) 2245
ScaI AGTACT 1 cut(s) 2434
SchI GAGTC 4 cut(s) 248, 434, 2327, 2885
ScrFI CCNGG 7 cut(s) 188, 242, 415, 682, 739, 1084, 2527
SdaI CCTGCAGG 1 cut(s) 2245
SduI GDGCHC 4 cut(s) 77, 306, 1234, 2561
SfcI CTRYAG 5 cut(s) 611, 1098, 1190, 2241, 2769
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 4 cut(s) 588, 1021, 1223, 2910
SmiMI CAYNNNNRTG 4 cut(s) 1092, 1409, 2874, 2880
SmlI CTYRAG 1 cut(s) 2952
SmoI CTYRAG 1 cut(s) 2952
SnaBI TACGTA 1 cut(s) 1547
SpeI ACTAGT 1 cut(s) 2650
Sse8387I CCTGCAGG 1 cut(s) 2245
SseBI AGGCCT 1 cut(s) 2235
SspMI CTAG 5 cut(s) 708, 852, 2651, 2820, 2946
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 2235
StyD4I CCNGG 7 cut(s) 186, 240, 413, 680, 737, 1082, 2525
StyI CCWWGG 5 cut(s) 1812, 2281, 2398, 2640, 2794
TaaI ACNGT 4 cut(s) 79, 1494, 2250, 2432
TaiI ACGT 9 cut(s) 38, 111, 327, 375, 1462, 1549, 2706, 2922, 3027
TaqII GACCGA 1 cut(s) 605
TatI WGTACW 4 cut(s) 1171, 1317, 1413, 2432
TauI GCSGC 6 cut(s) 26, 216, 380, 383, 2553, 2690
Tru1I TTAA 6 cut(s) 1140, 1764, 1772, 1797, 2846, 2997
Tru9I TTAA 6 cut(s) 1140, 1764, 1772, 1797, 2846, 2997
TscAI CASTG 9 cut(s) 45, 351, 869, 1199, 1219, 1558, 2310, 2728, 2836
TseFI GTSAC 5 cut(s) 36, 52, 1153, 2092, 2305
TseI GCWGC 5 cut(s) 471, 1097, 1100, 2260, 2620
Tsp45I GTSAC 5 cut(s) 36, 52, 1153, 2092, 2305
TspDTI ATGAA 3 cut(s) 1652, 2016, 2895
TspGWI ACGGA 5 cut(s) 71, 247, 1215, 1930, 2313
TspRI CASTG 9 cut(s) 45, 351, 869, 1199, 1219, 1558, 2310, 2728, 2836
Tth111I GACNNNGTC 2 cut(s) 257, 269
Van91I CCANNNNNTGG 1 cut(s) 1471
VpaK11BI GGWCC 4 cut(s) 588, 1021, 1223, 2910
XapI RAATTY 7 cut(s) 566, 1002, 1792, 1982, 2023, 2487, 2902
XceI RCATGY 7 cut(s) 851, 1097, 1172, 1501, 1511, 2521, 2873
XmiI GTMKAC 2 cut(s) 1741, 3046
XspI CTAG 5 cut(s) 708, 852, 2651, 2820, 2946
ZraI GACGTC 2 cut(s) 36, 109
ZrmI AGTACT 1 cut(s) 2434
Zsp2I ATGCAT 3 cut(s) 1612, 1846, 2735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.