RLG00000031082

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006279
Physical Location & Seq
Forward (+)
1847868 .. 1852517
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031082

Sequence Viewer

Length: 3048 bp
ATGGAGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCACTGAGATCGGAGGTGACGGACAAGTCGCAGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACGTCATCAACGGCCACCGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGCGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGACCTCGTCTTCTGCTCCTCCGATCAGAATGAGCAGGAGCTCCAAGACGACCAAAACGACGTCGTTTTCAGATTCGAGCCGCTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGCGTCGAGAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCTCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGACGCTTTTCTGGAGGCTCTGCGGAGTGAGGGCGGAGAGTTTATGGCTCCGGCTGGTGGAAATTACGAGAATGCCCCTTCCGGGTTGAAGGGAGATAATGGATGCTCTAGTCTGTCTGTTGTTCCAATAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTCTGGGGTCATTCGGCCTGCGCATTGGAGAAGAAGGTGGATAATGGGGTTCTTGTGTTTGGTGGATTTGGAGGCGTTGGAAGACATGCGAGGAGAAATCAGTGTTTTCTGGTTGATACATTGCCTGGTAGTTTGAGAGGGATTAGCGTAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGAGATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCGGAGAAAATTCTGAGTGATGTGTGGGTCCTCGATATACAGAAGATGGAATGGAAACTTGCGGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTTGTAGGATCAAAGATATATGTGTTTGGTGGACTTAACAATGATGCAGTCACTTCTTCCTTGCATGTACTGGAAGCAGATAATCTGCAGTGGGGAGAGATAATTGTCAGTGGGGAAGGTCCGTGTGCCCGACATTCACACTCAATGGTGGCATCGGGATCTCAGCTATATATGTTCGGAGGATATGATGGTGAGGAATCACTTGGAGACTTGTACAGGTTTGATACTGAAAAATCTAACTGTAAATGGAAAAAAGTGAAGGCGTCTGGGAGAAGTCCCCATGCAAGGTTCTCACACTCCATGTTTGTGTACAAAAATCATCTTGGAGTTATTGGTGGTTGTCCAGTTAGACAACATTGCCAAGAGTTGGCAATACTTGATCTACGGCTGTGCATGTGGAGGCATGTGAAACTAGAATCCACTGGTGAAGATTTGTTTGTACGTAGCACTGCCAATGTTGTTGGTGATGATCTCATTATGATTGGTGGCGGGGCTTCTTGTTACGCATTTGGTACAAAGTTCAGTAAGCCAATGAAAATCAACTTGCTTCCCCTGAAGCTTAGAGATGATAAACCACTTGTTAGAGAACGGCATACTGACCAAATTGATATTGTAAGGATCGAAAAGAGTAGACATCCACAGGTTCAAAATGTTAAAACATTAACTGAAGCTCCTGACCTAAATTTTAAGAGTAAATTACCCATGGCTGATGGCATTGGTCAACAGGTGGATGCATATTGGGTTCTGAAACTTGAAAGAAAGTATGCGAAAATAGGAAAGGACCTACTAAAGAAGTTTGGTTGGTTAGATCTTGCAAGGAAGGTTTACTCCGTGGAAGGTGGTTTACATATTTGTTTCCCTGTGAGTGCAAAATTTTCTGATGTATTGAAAGAAAATCAGCATCATGTGGCAGATTCATTAGAAGGACAGAGTGATCACATTTGTAAGCCAGATATAGGAAAGGATTGTTCGATTGATGAGGTTACCTGTTCAAAAGCCTTAGACATTCTAAAGGAATGTGGTGCCACGAAGCTGGTAGATGAGGTTGTTGAAGTCAGAAAAACTGCAAAATCTCCCTTGAAAATAATGAGTGATGCCGTGGGGTCTCTGCTAGAGGATAAAGGCGTACCTGCAGGACTGTTGGAGGAGCTGCCAACCAGATGGGAGAGACTTGGAGATATTGTTGTGCTTCCAGTGACATCATTTAAGAATCCATTATGGGACTCAATTGCTGAGGAGCTTTGGCCTGTCATTGCCAAATCAATAAATGCTGTTCGCCTTGCTCGTCAAGGCCGAGTTGCATCAACTGGTACAAGGGACAGTACTTTGGAGATTCTTATAGGAGATAATGGCTGGGTTGATCATCGTGAAAATGGAATTCTCTATTCTTTTGATGCTACTAAGTGCATGTTCTCCTGGGGCAACCTTTCTGAGAAGCTCCGCATGGGCACTCTGGATTGTAGAGATGAGATCATTGTGGATTTATTTGCTGGAATTGGATATTTTGTGCTGCCATTTCTTGTCAGGGCCAAGGCAAAACTAGTTTATGCTTGTGAATGGAATCCTCATGCTGTTGCGGCACTCCGGAGAAACATACAAGCCAATTCTGTCAGTGATCGATGCATCATTCTTGAAGGAGATAACCGGACAACAGCACCTACAGGAGTGGCTGATCGGGTCTGCCTTGGTCTCATTCCATCAAGTGAATCTAGCTGGGCCACAGCTGTTAGGGCATTAAGGGGTGAGGGTGGGATGCTACATGTGCATGGGAATGTGGTGGACTCAGAGGAGAATTCATGGACCAAGCACGTAACAGAATCAATAGCTGAAACTGCTAGATCTCAAGGTCGTTGTTGGGAGGTTTCAATAGAACATTTAGAGAGAGTGAAATGGTACGCCCCACACATCCGCCACGTTGTTGCAGATGTAAGATGTAGACAGACCCAGAAATAA

Protein Analysis

1016

Amino Acids

111.99

Weight (kDa)

6.67

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 205 2.6e-66 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 253 - 426 1.7e-39 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 299 - 393 1.6e-08 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 301 - 396 2.5e-20 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 302 - 349 4.3e-06 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 305 - 540 1.3e-25 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 307 - 347 1.5e-06 Kelch motif
Kelch_2 PF07646 307 - 348 8.3e-06 Kelch motif
Beta-prop_Calicin PF13964 318 - 449 2.6e-07 Calicin, beta-propeller domain
Kelch_HCF PF13854 333 - 382 1.8e-07 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 335 - 399 7.4e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 358 - 388 1e-05 Kelch motif
Beta-prop_FBX42 PF13415 359 - 530 1.9e-26 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 362 - 533 1.5e-38 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 394 - 481 3.9e-08 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 395 - 444 1.1e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 408 - 451 1.3e-06 Kelch motif
Kelch_2 PF07646 409 - 445 1.8e-06 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 421 - 535 1.1e-13 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 739 - 815 2.2e-14 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 820 - 970 8.7e-32 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 34, 61
AatII GACGTC 3 cut(s) 38, 111, 327
Acc16I TGCGCA 1 cut(s) 775
Acc36I ACCTGC 2 cut(s) 376, 2233
AccB1I GGYRCC 1 cut(s) 2117
AccB7I CCANNNNNTGG 1 cut(s) 1462
AccBSI CCGCTC 1 cut(s) 182
AccI GTMKAC 2 cut(s) 1726, 3031
AccII CGCG 5 cut(s) 126, 218, 464, 510, 528
AccIII TCCGGA 2 cut(s) 985, 2679
AclWI GGATC 5 cut(s) 242, 977, 1111, 1261, 1721
AcoI YGGCCR 3 cut(s) 118, 154, 243
AcsI RAATTY 6 cut(s) 566, 993, 1777, 1967, 2472, 2887
AcuI CTGAAG 2 cut(s) 1670, 1782
AcyI GRCGYC 5 cut(s) 35, 108, 324, 395, 1358
AfaI GTAC 9 cut(s) 1164, 1310, 1406, 1536, 1609, 2223, 2407, 2419, 2990
AfiI CCNNNNNNNGG 7 cut(s) 186, 647, 672, 926, 1380, 1462, 2051
AflIII ACRYGT 1 cut(s) 2854
AgsI TTSAA 9 cut(s) 679, 1742, 1850, 1984, 2088, 2147, 2176, 2729, 2961
AhlI ACTAGT 1 cut(s) 2635
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 3 cut(s) 877, 1073, 2510
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 6 cut(s) 196, 528, 1296, 2205, 2257, 2789
AlwI GGATC 5 cut(s) 242, 977, 1111, 1261, 1721
AlwNI CAGNNNCTG 2 cut(s) 147, 391
Aor13HI TCCGGA 2 cut(s) 985, 2679
ApeKI GCWGC 5 cut(s) 471, 1088, 1091, 2245, 2605
ApoI RAATTY 6 cut(s) 566, 993, 1777, 1967, 2472, 2887
AspLEI GCGC 3 cut(s) 218, 385, 776
AspS9I GGNCC 8 cut(s) 588, 928, 1012, 1214, 1876, 2622, 2811, 2895
AsuC2I CCSGG 5 cut(s) 188, 242, 415, 673, 730
AsuHPI GGTGA 8 cut(s) 64, 127, 909, 1070, 1298, 1532, 1571, 2849
AsuII TTCGAA 1 cut(s) 443
AvaII GGWCC 5 cut(s) 588, 1012, 1214, 1876, 2895
BaeGI GKGCMC 3 cut(s) 77, 1225, 2546
BanI GGYRCC 1 cut(s) 2117
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 1923, 1955
BbsI GAAGAC 2 cut(s) 265, 841
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 2328
BbvI GCAGC 5 cut(s) 458, 1075, 1103, 2232, 2592
BccI CCATC 6 cut(s) 173, 1024, 1277, 1799, 2250, 2800
BceAI ACGGC 7 cut(s) 133, 341, 951, 1079, 1496, 1700, 2177
BciT130I CCWGG 3 cut(s) 879, 1075, 2512
BclI TGATCA 2 cut(s) 2029, 2455
BcnI CCSGG 5 cut(s) 188, 242, 415, 673, 730
BcoDI GTCTC 6 cut(s) 196, 528, 1296, 2205, 2257, 2789
BcuI ACTAGT 1 cut(s) 2635
BfaI CTAG 6 cut(s) 699, 1508, 2207, 2636, 2805, 2931
BfmI CTRYAG 4 cut(s) 1089, 1181, 2226, 2754
BfuAI ACCTGC 2 cut(s) 376, 2233
BglII AGATCT 2 cut(s) 1903, 2933
BmcAI AGTACT 1 cut(s) 2419
Bme1390I CCNGG 8 cut(s) 188, 242, 415, 673, 730, 879, 1075, 2512
Bme18I GGWCC 5 cut(s) 588, 1012, 1214, 1876, 2895
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 8 cut(s) 588, 928, 1012, 1214, 1876, 2622, 2811, 2895
BmiI GGNNCC 5 cut(s) 492, 542, 639, 1013, 2119
BmrFI CCNGG 8 cut(s) 188, 242, 415, 673, 730, 879, 1075, 2512
BpiI GAAGAC 2 cut(s) 265, 841
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 2328
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 2922
BpuMI CCSGG 5 cut(s) 188, 242, 415, 673, 730
Bsa29I ATCGAT 1 cut(s) 2713
BsaAI YACGTR 2 cut(s) 1538, 2905
BsaHI GRCGYC 5 cut(s) 35, 108, 324, 395, 1358
BsaI GGTCTC 3 cut(s) 196, 2205, 2789
BsaJI CCNNGG 8 cut(s) 729, 1073, 1797, 1926, 2193, 2511, 2625, 2779
BsaWI WCCGGW 4 cut(s) 579, 985, 2679, 2739
BsaXI ACNNNNNCTCC 6 cut(s) 942, 972, 1750, 1780, 2262, 2292
Bsc4I CCNNNNNNNGG 7 cut(s) 186, 647, 672, 926, 1380, 1462, 2051
Bse1I ACTGG 5 cut(s) 1170, 1439, 1522, 2288, 2407
Bse3DI GCAATG 3 cut(s) 872, 1450, 2346
BseAI TCCGGA 2 cut(s) 985, 2679
BseBI CCWGG 3 cut(s) 879, 1075, 2512
BseCI ATCGAT 1 cut(s) 2713
BseDI CCNNGG 8 cut(s) 729, 1073, 1797, 1926, 2193, 2511, 2625, 2779
BseGI GGATG 6 cut(s) 165, 698, 1729, 1831, 2853, 3000
BseLI CCNNNNNNNGG 7 cut(s) 186, 647, 672, 926, 1380, 1462, 2051
BseMI GCAATG 3 cut(s) 872, 1450, 2346
BseMII CTCAG 7 cut(s) 32, 188, 989, 1271, 2319, 2517, 2892
BseNI ACTGG 5 cut(s) 1170, 1439, 1522, 2288, 2407
BseRI GAGGAG 7 cut(s) 92, 271, 755, 859, 2255, 2345, 2897
BseSI GKGCMC 3 cut(s) 77, 1225, 2546
BseX3I CGGCCG 2 cut(s) 154, 243
BseXI GCAGC 5 cut(s) 458, 1075, 1103, 2232, 2592
BseYI CCCAGC 2 cut(s) 2448, 2808
BsgI GTGCAG 1 cut(s) 385
Bsh1236I CGCG 5 cut(s) 126, 218, 464, 510, 528
Bsh1285I CGRYCG 2 cut(s) 157, 246
BshNI GGYRCC 1 cut(s) 2117
BshVI ATCGAT 1 cut(s) 2713
BsiEI CGRYCG 2 cut(s) 157, 246
BsiHKAI GWGCWC 1 cut(s) 306
BslFI GGGAC 4 cut(s) 383, 1356, 2330, 2426
BslI CCNNNNNNNGG 7 cut(s) 186, 647, 672, 926, 1380, 1462, 2051
BsmAI GTCTC 6 cut(s) 196, 528, 1296, 2205, 2257, 2789
BsmFI GGGAC 4 cut(s) 383, 1356, 2330, 2426
BsmI GAATGC 1 cut(s) 667
Bso31I GGTCTC 3 cut(s) 196, 2205, 2789
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 4 cut(s) 77, 306, 1225, 2546
Bsp13I TCCGGA 2 cut(s) 985, 2679
Bsp1407I TGTACA 2 cut(s) 1308, 1404
Bsp19I CCATGG 1 cut(s) 1797
Bsp68I TCGCGA 3 cut(s) 464, 510, 528
BspCNI CTCAG 7 cut(s) 33, 187, 990, 1270, 2320, 2518, 2891
BspDI ATCGAT 1 cut(s) 2713
BspEI TCCGGA 2 cut(s) 985, 2679
BspFNI CGCG 5 cut(s) 126, 218, 464, 510, 528
BspLI GGNNCC 5 cut(s) 492, 542, 639, 1013, 2119
BspMAI CTGCAG 3 cut(s) 1093, 1185, 2230
BspMI ACCTGC 2 cut(s) 376, 2233
BspPI GGATC 5 cut(s) 242, 977, 1111, 1261, 1721
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 2117
BspTNI GGTCTC 3 cut(s) 196, 2205, 2789
BsrBI CCGCTC 1 cut(s) 182
BsrDI GCAATG 3 cut(s) 872, 1450, 2346
BsrGI TGTACA 2 cut(s) 1308, 1404
BsrI ACTGG 5 cut(s) 1170, 1439, 1522, 2288, 2407
BssECI CCNNGG 8 cut(s) 729, 1073, 1797, 1926, 2193, 2511, 2625, 2779
BssNI GRCGYC 5 cut(s) 35, 108, 324, 395, 1358
BssT1I CCWWGG 3 cut(s) 1797, 2625, 2779
Bst2UI CCWGG 3 cut(s) 879, 1075, 2512
Bst4CI ACNGT 4 cut(s) 79, 1337, 2235, 2417
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 5 cut(s) 35, 108, 324, 395, 1358
BstAUI TGTACA 2 cut(s) 1308, 1404
BstBAI YACGTR 2 cut(s) 1538, 2905
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 180, 184, 772
BstDSI CCRYGG 3 cut(s) 1797, 1926, 2193
BstEII GGTNACC 1 cut(s) 2077
BstF5I GGATG 6 cut(s) 165, 698, 1729, 1831, 2853, 3000
BstFNI CGCG 5 cut(s) 126, 218, 464, 510, 528
BstHHI GCGC 3 cut(s) 218, 385, 776
BstMAI GTCTC 6 cut(s) 196, 528, 1296, 2205, 2257, 2789
BstMCI CGRYCG 2 cut(s) 157, 246
BstMWI GCNNNNNNNGC 5 cut(s) 401, 2672, 2825, 2857, 2927
BstNI CCWGG 3 cut(s) 879, 1075, 2512
BstNSI RCATGY 7 cut(s) 842, 1088, 1163, 1492, 1502, 2506, 2858
BstPI GGTNACC 1 cut(s) 2077
BstSCI CCNGG 8 cut(s) 186, 240, 413, 671, 728, 877, 1073, 2510
BstSFI CTRYAG 4 cut(s) 1089, 1181, 2226, 2754
BstSLI GKGCMC 3 cut(s) 77, 1225, 2546
BstSNI TACGTA 1 cut(s) 1538
BstUI CGCG 5 cut(s) 126, 218, 464, 510, 528
BstV1I GCAGC 5 cut(s) 458, 1075, 1103, 2232, 2592
BstV2I GAAGAC 2 cut(s) 265, 841
BstX2I RGATCY 3 cut(s) 1253, 1903, 2933
BstXI CCANNNNNNTGG 2 cut(s) 2128, 2256
BstYI RGATCY 3 cut(s) 1253, 1903, 2933
BstZI CGGCCG 2 cut(s) 154, 243
Bsu15I ATCGAT 1 cut(s) 2713
BsuTUI ATCGAT 1 cut(s) 2713
BtgI CCRYGG 3 cut(s) 1797, 1926, 2193
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 6 cut(s) 165, 698, 1729, 1831, 2853, 3000
BtsI GCAGTG 2 cut(s) 1190, 1542
BtsIMutI CAGTG 8 cut(s) 38, 860, 1190, 1210, 1515, 1542, 2295, 2713
BtuMI TCGCGA 3 cut(s) 464, 510, 528
BveI ACCTGC 2 cut(s) 376, 2233
Cac8I GCNNGC 3 cut(s) 180, 184, 772
CaiI CAGNNNCTG 2 cut(s) 147, 391
CfoI GCGC 3 cut(s) 218, 385, 776
Cfr13I GGNCC 8 cut(s) 588, 928, 1012, 1214, 1876, 2622, 2811, 2895
ClaI ATCGAT 1 cut(s) 2713
CseI GACGC 5 cut(s) 37, 384, 399, 602, 1347
Csp6I GTAC 9 cut(s) 1163, 1309, 1405, 1535, 1608, 2222, 2406, 2418, 2989
CviQI GTAC 9 cut(s) 1163, 1309, 1405, 1535, 1608, 2222, 2406, 2418, 2989
DrdI GACNNNNNNGTC 2 cut(s) 34, 61
DseDI GACNNNNNNGTC 2 cut(s) 34, 61
EaeI YGGCCR 3 cut(s) 118, 154, 243
EagI CGGCCG 2 cut(s) 154, 243
Eam1104I CTCTTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 578
EciI GGCGGA 2 cut(s) 639, 2993
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 2 cut(s) 154, 243
Eco105I TACGTA 1 cut(s) 1538
Eco130I CCWWGG 3 cut(s) 1797, 2625, 2779
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 3 cut(s) 196, 2205, 2789
Eco47I GGWCC 5 cut(s) 588, 1012, 1214, 1876, 2895
Eco52I CGGCCG 2 cut(s) 154, 243
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 2 cut(s) 1670, 1782
Eco91I GGTNACC 1 cut(s) 2077
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 2 cut(s) 1012, 1876
EcoO65I GGTNACC 1 cut(s) 2077
EcoRI GAATTC 2 cut(s) 2472, 2887
EcoRII CCWGG 3 cut(s) 877, 1073, 2510
EcoT14I CCWWGG 3 cut(s) 1797, 2625, 2779
EcoT22I ATGCAT 2 cut(s) 1831, 2720
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 3 cut(s) 1797, 2625, 2779
FaqI GGGAC 4 cut(s) 383, 1356, 2330, 2426
FauI CCCGC 2 cut(s) 187, 1577
FbaI TGATCA 2 cut(s) 2029, 2455
FblI GTMKAC 2 cut(s) 1726, 3031
FokI GGATG 6 cut(s) 152, 705, 1716, 1838, 2860, 2987
FriOI GRGCYC 1 cut(s) 306
FspBI CTAG 6 cut(s) 699, 1508, 2207, 2636, 2805, 2931
FspI TGCGCA 1 cut(s) 775
GlaI GCGC 3 cut(s) 217, 384, 775
GsaI CCCAGC 2 cut(s) 2452, 2812
GsuI CTGGAG 1 cut(s) 623
HgaI GACGC 5 cut(s) 37, 384, 399, 602, 1347
HhaI GCGC 3 cut(s) 218, 385, 776
Hin1I GRCGYC 5 cut(s) 35, 108, 324, 395, 1358
Hin6I GCGC 3 cut(s) 216, 383, 774
HinP1I GCGC 3 cut(s) 216, 383, 774
HincII GTYRAC 1 cut(s) 1817
HindII GTYRAC 1 cut(s) 1817
HindIII AAGCTT 1 cut(s) 1652
HphI GGTGA 8 cut(s) 64, 127, 909, 1070, 1298, 1532, 1571, 2849
Hpy166II GTNNAC 9 cut(s) 82, 1127, 1405, 1727, 1817, 1921, 1940, 2875, 3032
Hpy8I GTNNAC 9 cut(s) 82, 1127, 1405, 1727, 1817, 1921, 1940, 2875, 3032
Hpy99I CGWCG 7 cut(s) 31, 110, 326, 329, 362, 400, 596
HpyCH4III ACNGT 4 cut(s) 79, 1337, 2235, 2417
HpyCH4IV ACGT 7 cut(s) 35, 108, 324, 372, 1537, 2904, 3009
HpyF10VI GCNNNNNNNGC 5 cut(s) 401, 2672, 2825, 2857, 2927
HpySE526I ACGT 7 cut(s) 35, 108, 324, 372, 1537, 2904, 3009
Hsp92I GRCGYC 5 cut(s) 35, 108, 324, 395, 1358
HspAI GCGC 3 cut(s) 216, 383, 774
Kpn2I TCCGGA 2 cut(s) 985, 2679
Ksp22I TGATCA 2 cut(s) 2029, 2455
Lsp1109I GCAGC 5 cut(s) 458, 1075, 1103, 2232, 2592
MaeI CTAG 6 cut(s) 699, 1508, 2207, 2636, 2805, 2931
MaeII ACGT 7 cut(s) 35, 108, 324, 372, 1537, 2904, 3009
MaeIII GTNAC 7 cut(s) 36, 52, 1144, 1595, 2077, 2290, 2905
MbiI CCGCTC 1 cut(s) 182
MboII GAAGA 8 cut(s) 211, 265, 595, 796, 846, 1039, 1143, 1535
MfeI CAATTG 1 cut(s) 2322
MflI RGATCY 3 cut(s) 1253, 1903, 2933
MhlI GDGCHC 4 cut(s) 77, 306, 1225, 2546
MlyI GAGTC 4 cut(s) 248, 434, 2312, 2870
MmeI TCCRAC 3 cut(s) 811, 928, 2217
Mph1103I ATGCAT 2 cut(s) 1831, 2720
MroI TCCGGA 2 cut(s) 985, 2679
MseI TTAA 6 cut(s) 1131, 1749, 1757, 1782, 2301, 2831
MslI CAYNNNNRTG 4 cut(s) 1083, 1400, 2859, 2865
MspA1I CMGCKG 2 cut(s) 346, 2819
MspR9I CCNGG 8 cut(s) 188, 242, 415, 673, 730, 879, 1075, 2512
MunI CAATTG 1 cut(s) 2322
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 3 cut(s) 879, 1075, 2512
MvnI CGCG 5 cut(s) 126, 218, 464, 510, 528
MwoI GCNNNNNNNGC 5 cut(s) 401, 2672, 2825, 2857, 2927
NciI CCSGG 5 cut(s) 188, 242, 415, 673, 730
NcoI CCATGG 1 cut(s) 1797
NlaIV GGNNCC 5 cut(s) 492, 542, 639, 1013, 2119
NmeAIII GCCGAG 1 cut(s) 2414
NmuCI GTSAC 4 cut(s) 36, 52, 1144, 2290
NruI TCGCGA 3 cut(s) 464, 510, 528
NsbI TGCGCA 1 cut(s) 775
NsiI ATGCAT 2 cut(s) 1831, 2720
NspI RCATGY 7 cut(s) 842, 1088, 1163, 1492, 1502, 2506, 2858
NspV TTCGAA 1 cut(s) 443
PciI ACATGT 1 cut(s) 2854
PcsI WCGNNNNNNNCGW 6 cut(s) 123, 243, 318, 567, 2377, 2386
PctI GAATGC 1 cut(s) 667
PflFI GACNNNGTC 2 cut(s) 257, 269
PflMI CCANNNNNTGG 1 cut(s) 1462
PleI GAGTC 4 cut(s) 248, 433, 2312, 2870
PpsI GAGTC 4 cut(s) 248, 433, 2312, 2870
Ppu21I YACGTR 2 cut(s) 1538, 2905
PpuMI RGGWCCY 2 cut(s) 1012, 1876
PscI ACATGT 1 cut(s) 2854
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 2 cut(s) 1012, 1876
Psp6I CCWGG 3 cut(s) 877, 1073, 2510
PspEI GGTNACC 1 cut(s) 2077
PspFI CCCAGC 2 cut(s) 2448, 2808
PspGI CCWGG 3 cut(s) 877, 1073, 2510
PspN4I GGNNCC 5 cut(s) 492, 542, 639, 1013, 2119
PspPI GGNCC 8 cut(s) 588, 928, 1012, 1214, 1876, 2622, 2811, 2895
PspPPI RGGWCCY 2 cut(s) 1012, 1876
PsrI GAACNNNNNNTAC 2 cut(s) 524, 556
PstI CTGCAG 3 cut(s) 1093, 1185, 2230
PstNI CAGNNNCTG 2 cut(s) 147, 391
PsuI RGATCY 3 cut(s) 1253, 1903, 2933
PsyI GACNNNGTC 2 cut(s) 257, 269
PvuII CAGCTG 1 cut(s) 2819
RruI TCGCGA 3 cut(s) 464, 510, 528
RsaI GTAC 9 cut(s) 1164, 1310, 1406, 1536, 1609, 2223, 2407, 2419, 2990
RsaNI GTAC 9 cut(s) 1163, 1309, 1405, 1535, 1608, 2222, 2406, 2418, 2989
RseI CAYNNNNRTG 4 cut(s) 1083, 1400, 2859, 2865
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 6 cut(s) 1131, 1749, 1757, 1782, 2301, 2831
Sau96I GGNCC 8 cut(s) 588, 928, 1012, 1214, 1876, 2622, 2811, 2895
SbfI CCTGCAGG 1 cut(s) 2230
ScaI AGTACT 1 cut(s) 2419
SchI GAGTC 4 cut(s) 248, 434, 2312, 2870
ScrFI CCNGG 8 cut(s) 188, 242, 415, 673, 730, 879, 1075, 2512
SdaI CCTGCAGG 1 cut(s) 2230
SduI GDGCHC 4 cut(s) 77, 306, 1225, 2546
SfcI CTRYAG 4 cut(s) 1089, 1181, 2226, 2754
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 5 cut(s) 588, 1012, 1214, 1876, 2895
SmiMI CAYNNNNRTG 4 cut(s) 1083, 1400, 2859, 2865
SmlI CTYRAG 1 cut(s) 2937
SmoI CTYRAG 1 cut(s) 2937
SnaBI TACGTA 1 cut(s) 1538
SpeI ACTAGT 1 cut(s) 2635
Sse8387I CCTGCAGG 1 cut(s) 2230
SspMI CTAG 6 cut(s) 699, 1508, 2207, 2636, 2805, 2931
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 8 cut(s) 186, 240, 413, 671, 728, 877, 1073, 2510
StyI CCWWGG 3 cut(s) 1797, 2625, 2779
TaaI ACNGT 4 cut(s) 79, 1337, 2235, 2417
TaiI ACGT 7 cut(s) 38, 111, 327, 375, 1540, 2907, 3012
TaqII GACCGA 1 cut(s) 605
TatI WGTACW 4 cut(s) 1162, 1308, 1404, 2417
TauI GCSGC 6 cut(s) 26, 216, 346, 380, 383, 2675
Tru1I TTAA 6 cut(s) 1131, 1749, 1757, 1782, 2301, 2831
Tru9I TTAA 6 cut(s) 1131, 1749, 1757, 1782, 2301, 2831
TscAI CASTG 8 cut(s) 45, 860, 1190, 1210, 1522, 1549, 2295, 2713
TseFI GTSAC 4 cut(s) 36, 52, 1144, 2290
TseI GCWGC 5 cut(s) 471, 1088, 1091, 2245, 2605
Tsp45I GTSAC 4 cut(s) 36, 52, 1144, 2290
TspDTI ATGAA 3 cut(s) 1643, 2001, 2880
TspGWI ACGGA 4 cut(s) 71, 247, 1206, 1915
TspRI CASTG 8 cut(s) 45, 860, 1190, 1210, 1522, 1549, 2295, 2713
Tth111I GACNNNGTC 2 cut(s) 257, 269
Van91I CCANNNNNTGG 1 cut(s) 1462
VpaK11BI GGWCC 5 cut(s) 588, 1012, 1214, 1876, 2895
XapI RAATTY 6 cut(s) 566, 993, 1777, 1967, 2472, 2887
XceI RCATGY 7 cut(s) 842, 1088, 1163, 1492, 1502, 2506, 2858
XmiI GTMKAC 2 cut(s) 1726, 3031
XspI CTAG 6 cut(s) 699, 1508, 2207, 2636, 2805, 2931
ZraI GACGTC 3 cut(s) 36, 109, 325
ZrmI AGTACT 1 cut(s) 2419
Zsp2I ATGCAT 2 cut(s) 1831, 2720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.