Rw5G002390

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006279
Physical Location & Seq
Reverse (-)
2105697 .. 2107051
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002390.1

Sequence Viewer

Length: 957 bp
ATGGTGTTCGAGAAGCGAAAAGCGGCGACGCTGACGTCACTGAGATCGGAGGTGACGGACAAGTCGCCGAAGGGCACGGTGGACACTCCGATCATTCCTCTCCTCGACCTCATCAACGGCCACCGCGACTACTTCACCACCAGCTCCTGCTCCGGCCGAATCTCCATCCTCTCTCAGCCCGCTCGCCGGGTCTCCAAGAAGAAAGCCTCCGGCGGCGGGTGGCTTTACATCTCTCACGACCCGGCCGATCCCGACTCCGTCCTCGACCTCCTCCAAAACGACCAAAACGACGTTGTTTTCAGATTCGAGCCACTGATTATCGCCGTCGAGTGCAGGGACGTGGCGGCGGCGCAGGTTCTGGTGTCGAAAGCCATAGCGTCCGGGTTCAGAGAGTCCGGCATTACGAGTTCGAACAAGCGTGTGATTATCGCGATTCGCTGCTCGATACGATTGGAGGTTCCGCTGGGGAGTAGTCGCGAGATTATGGTGTCTCGCGAGTATTTGAGGTTCCTTGTGGGCGTGGCGAACGAGAAATTCGAGGCCAACCGGAAGAGGACCGAGGCTTTTCTGGAGGCTCTGCAGAGTGGGGAGAGCGGAGGAGGGTTGAAGGGAGATAATGGATGCTCTAGTCTGTCTGTTGTTCCGATAGAGGTTTCCGGGGAGAATGAGGAGAATCTTTACCTTTGGGGTCATTCGGCTTGCCGAAATCAGTGTTTGCTGGTTGATACATTGTCTGGTAGTTTGAAAGGGATTAGCGTAGAGAGTAGTCCTTCACCGAGATTGGGCCACACGGCTTCTTTGGTTGGAGATTGTGTGTTTGTTATTGGAGGCAGGGCTGATCCTGAGAAAATTCTGAGTGATGTATGGGTCCTCGATATACAGAAGAAGGAATGGAAACTCGCGAATGTTCTGGTGATGTATTTCCTCCCAGGCATCGACATGCTGCAGCCGTGGTAG

Protein Analysis

318

Amino Acids

34.72

Weight (kDa)

6.46

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 5 - 194 1.5e-65 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 223 - 301 2.6e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 247 - 300 2.4e-10 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 252 - 302 1.9e-07 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 257 - 301 1.1e-08 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_1 PF01344 259 - 300 6.6e-09 Kelch motif
Kelch_2 PF07646 259 - 302 7e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 34, 61
AatII GACGTC 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 343
AccBSI CCGCTC 2 cut(s) 182, 594
AccII CGCG 5 cut(s) 126, 431, 477, 495, 902
AciI CCGC 9 cut(s) 23, 124, 180, 213, 216, 344, 347, 461, 594
AclWI GGATC 2 cut(s) 242, 833
AcoI YGGCCR 3 cut(s) 118, 154, 243
AcsI RAATTY 2 cut(s) 533, 849
AcyI GRCGYC 1 cut(s) 35
AfiI CCNNNNNNNGG 3 cut(s) 186, 216, 782
AgsI TTSAA 2 cut(s) 607, 745
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 1 cut(s) 928
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 196, 495
AlwI GGATC 2 cut(s) 242, 833
AlwNI CAGNNNCTG 2 cut(s) 147, 358
AoxI GGCC 5 cut(s) 118, 154, 243, 540, 784
ApeKI GCWGC 3 cut(s) 438, 943, 946
ApoI RAATTY 2 cut(s) 533, 849
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 3 cut(s) 555, 784, 868
AsuC2I CCSGG 4 cut(s) 188, 242, 382, 658
AsuHPI GGTGA 4 cut(s) 64, 127, 765, 925
AsuII TTCGAA 1 cut(s) 410
AvaII GGWCC 2 cut(s) 555, 868
BaeGI GKGCMC 1 cut(s) 77
BbvI GCAGC 2 cut(s) 425, 930
BccI CCATC 1 cut(s) 173
BceAI ACGGC 4 cut(s) 133, 308, 807, 934
BciT130I CCWGG 1 cut(s) 930
BcnI CCSGG 4 cut(s) 188, 242, 382, 658
BcoDI GTCTC 2 cut(s) 196, 495
BfaI CTAG 1 cut(s) 627
BfmI CTRYAG 2 cut(s) 578, 944
BfuAI ACCTGC 1 cut(s) 343
BisI GCNGC 7 cut(s) 24, 214, 345, 348, 439, 944, 947
BlsI GCNGC 7 cut(s) 25, 215, 346, 349, 440, 945, 948
Bme1390I CCNGG 5 cut(s) 188, 242, 382, 658, 930
Bme18I GGWCC 2 cut(s) 555, 868
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 3 cut(s) 555, 784, 868
BmiI GGNNCC 3 cut(s) 459, 509, 869
BmrFI CCNGG 5 cut(s) 188, 242, 382, 658, 930
BmsI GCATC 2 cut(s) 611, 942
BpmI CTGGAG 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 410
BpuMI CCSGG 4 cut(s) 188, 242, 382, 658
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 4 cut(s) 558, 657, 928, 950
BsaWI WCCGGW 1 cut(s) 546
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 216, 782
BseBI CCWGG 1 cut(s) 930
BseDI CCNNGG 4 cut(s) 558, 657, 928, 950
BseGI GGATG 2 cut(s) 165, 626
BseLI CCNNNNNNNGG 3 cut(s) 186, 216, 782
BseMII CTCAG 4 cut(s) 32, 188, 834, 845
BseRI GAGGAG 4 cut(s) 92, 260, 612, 683
BseSI GKGCMC 1 cut(s) 77
BseX3I CGGCCG 2 cut(s) 154, 243
BseXI GCAGC 2 cut(s) 425, 930
BseYI CCCAGC 1 cut(s) 463
BsgI GTGCAG 1 cut(s) 352
Bsh1236I CGCG 5 cut(s) 126, 431, 477, 495, 902
Bsh1285I CGRYCG 2 cut(s) 157, 246
BshFI GGCC 5 cut(s) 120, 156, 245, 542, 786
BsiEI CGRYCG 2 cut(s) 157, 246
BsiSI CCGG 8 cut(s) 153, 187, 210, 242, 381, 396, 547, 657
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 186, 216, 782
BsmAI GTCTC 2 cut(s) 196, 495
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 5 cut(s) 120, 156, 245, 542, 786
Bso31I GGTCTC 1 cut(s) 196
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 4 cut(s) 44, 90, 247, 838
Bsp68I TCGCGA 4 cut(s) 431, 477, 495, 902
BspACI CCGC 9 cut(s) 23, 124, 180, 213, 216, 344, 347, 461, 594
BspANI GGCC 5 cut(s) 120, 156, 245, 542, 786
BspCNI CTCAG 4 cut(s) 33, 187, 835, 846
BspFNI CGCG 5 cut(s) 126, 431, 477, 495, 902
BspLI GGNNCC 3 cut(s) 459, 509, 869
BspMAI CTGCAG 2 cut(s) 582, 948
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 2 cut(s) 242, 833
BspT104I TTCGAA 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 196
BsrBI CCGCTC 2 cut(s) 182, 594
BssECI CCNNGG 4 cut(s) 558, 657, 928, 950
BssMI GATC 4 cut(s) 44, 90, 247, 838
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 930
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 545
BstACI GRCGYC 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 180, 184, 700
BstDEI CTNAG 4 cut(s) 41, 174, 843, 854
BstDSI CCRYGG 1 cut(s) 950
BstF5I GGATG 2 cut(s) 165, 626
BstFNI CGCG 5 cut(s) 126, 431, 477, 495, 902
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 4 cut(s) 47, 93, 250, 841
BstMAI GTCTC 2 cut(s) 196, 495
BstMBI GATC 4 cut(s) 44, 90, 247, 838
BstMCI CGRYCG 2 cut(s) 157, 246
BstNI CCWGG 1 cut(s) 930
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 5 cut(s) 186, 240, 380, 656, 928
BstSFI CTRYAG 2 cut(s) 578, 944
BstSLI GKGCMC 1 cut(s) 77
BstUI CGCG 5 cut(s) 126, 431, 477, 495, 902
BstV1I GCAGC 2 cut(s) 425, 930
BstZI CGGCCG 2 cut(s) 154, 243
BsuRI GGCC 5 cut(s) 120, 156, 245, 542, 786
BtgI CCRYGG 1 cut(s) 950
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 2 cut(s) 165, 626
BtsIMutI CAGTG 3 cut(s) 38, 311, 716
BtuMI TCGCGA 4 cut(s) 431, 477, 495, 902
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 3 cut(s) 180, 184, 700
CaiI CAGNNNCTG 2 cut(s) 147, 358
CfoI GCGC 1 cut(s) 352
Cfr13I GGNCC 3 cut(s) 555, 784, 868
CseI GACGC 2 cut(s) 37, 366
CviAII CATG 1 cut(s) 940
DdeI CTNAG 4 cut(s) 41, 174, 843, 854
DpnI GATC 4 cut(s) 46, 92, 249, 840
DpnII GATC 4 cut(s) 44, 90, 247, 838
DrdI GACNNNNNNGTC 2 cut(s) 34, 61
DseDI GACNNNNNNGTC 2 cut(s) 34, 61
EaeI YGGCCR 3 cut(s) 118, 154, 243
EagI CGGCCG 2 cut(s) 154, 243
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
EclXI CGGCCG 2 cut(s) 154, 243
Eco31I GGTCTC 1 cut(s) 196
Eco47I GGWCC 2 cut(s) 555, 868
Eco52I CGGCCG 2 cut(s) 154, 243
EcoO109I RGGNCCY 1 cut(s) 868
EcoRII CCWGG 1 cut(s) 928
FaeI CATG 1 cut(s) 943
FaiI YATR 5 cut(s) 374, 485, 865, 878, 941
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 939
FauI CCCGC 2 cut(s) 187, 209
Fnu4HI GCNGC 7 cut(s) 24, 214, 345, 348, 439, 944, 947
FokI GGATG 2 cut(s) 152, 633
Fsp4HI GCNGC 7 cut(s) 24, 214, 345, 348, 439, 944, 947
FspBI CTAG 1 cut(s) 627
GlaI GCGC 1 cut(s) 351
GluI GCNGC 7 cut(s) 24, 214, 345, 348, 439, 944, 947
GsaI CCCAGC 1 cut(s) 467
GsuI CTGGAG 1 cut(s) 590
HaeIII GGCC 5 cut(s) 120, 156, 245, 542, 786
HapII CCGG 8 cut(s) 153, 187, 210, 242, 381, 396, 547, 657
HgaI GACGC 2 cut(s) 37, 366
HhaI GCGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 1 cut(s) 943
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HinfI GANTC 6 cut(s) 159, 254, 303, 392, 433, 673
HpaII CCGG 8 cut(s) 153, 187, 210, 242, 381, 396, 547, 657
HphI GGTGA 4 cut(s) 64, 127, 765, 925
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 6 cut(s) 49, 90, 302, 389, 645, 855
Hpy188III TCNNGA 9 cut(s) 10, 236, 251, 430, 476, 494, 569, 842, 901
Hpy8I GTNNAC 1 cut(s) 82
Hpy99I CGWCG 3 cut(s) 31, 293, 329
HpyAV CCTTC 4 cut(s) 64, 601, 780, 880
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 3 cut(s) 35, 291, 339
HpyCH4V TGCA 3 cut(s) 333, 580, 946
HpyF3I CTNAG 4 cut(s) 41, 174, 843, 854
HpySE526I ACGT 3 cut(s) 35, 291, 339
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 1 cut(s) 943
HspAI GCGC 1 cut(s) 350
Kzo9I GATC 4 cut(s) 44, 90, 247, 838
LmnI GCTCC 2 cut(s) 149, 155
Lsp1109I GCAGC 2 cut(s) 425, 930
LweI GCATC 2 cut(s) 611, 942
MaeI CTAG 1 cut(s) 627
MaeII ACGT 3 cut(s) 35, 291, 339
MaeIII GTNAC 2 cut(s) 36, 52
MalI GATC 4 cut(s) 46, 92, 249, 840
MbiI CCGCTC 2 cut(s) 182, 594
MboI GATC 4 cut(s) 44, 90, 247, 838
MboII GAAGA 3 cut(s) 211, 562, 895
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 2 cut(s) 533, 849
MlyI GAGTC 2 cut(s) 248, 401
MmeI TCCRAC 1 cut(s) 784
MslI CAYNNNNRTG 1 cut(s) 938
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 8 cut(s) 153, 187, 210, 242, 381, 396, 547, 657
MspR9I CCNGG 5 cut(s) 188, 242, 382, 658, 930
MvaI CCWGG 1 cut(s) 930
MvnI CGCG 5 cut(s) 126, 431, 477, 495, 902
NciI CCSGG 4 cut(s) 188, 242, 382, 658
NdeII GATC 4 cut(s) 44, 90, 247, 838
NlaIII CATG 1 cut(s) 943
NlaIV GGNNCC 3 cut(s) 459, 509, 869
NmuCI GTSAC 2 cut(s) 36, 52
NruI TCGCGA 4 cut(s) 431, 477, 495, 902
NspI RCATGY 1 cut(s) 943
NspV TTCGAA 1 cut(s) 410
PcsI WCGNNNNNNNCGW 4 cut(s) 123, 243, 285, 534
PfeI GAWTC 4 cut(s) 159, 303, 433, 673
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 7 cut(s) 25, 215, 346, 349, 440, 945, 948
PleI GAGTC 2 cut(s) 248, 400
PpsI GAGTC 2 cut(s) 248, 400
PpuMI RGGWCCY 1 cut(s) 868
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 1 cut(s) 928
PspFI CCCAGC 1 cut(s) 463
PspGI CCWGG 1 cut(s) 928
PspN4I GGNNCC 3 cut(s) 459, 509, 869
PspPI GGNCC 3 cut(s) 555, 784, 868
PspPPI RGGWCCY 1 cut(s) 868
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PstI CTGCAG 2 cut(s) 582, 948
PstNI CAGNNNCTG 2 cut(s) 147, 358
PsyI GACNNNGTC 1 cut(s) 257
RruI TCGCGA 4 cut(s) 431, 477, 495, 902
RseI CAYNNNNRTG 1 cut(s) 938
SatI GCNGC 7 cut(s) 24, 214, 345, 348, 439, 944, 947
Sau3AI GATC 4 cut(s) 44, 90, 247, 838
Sau96I GGNCC 3 cut(s) 555, 784, 868
SchI GAGTC 2 cut(s) 248, 401
ScrFI CCNGG 5 cut(s) 188, 242, 382, 658, 930
SduI GDGCHC 1 cut(s) 77
SfaNI GCATC 2 cut(s) 611, 942
SfcI CTRYAG 2 cut(s) 578, 944
SfuI TTCGAA 1 cut(s) 410
SinI GGWCC 2 cut(s) 555, 868
SmiMI CAYNNNNRTG 1 cut(s) 938
Sse9I AATT 2 cut(s) 533, 849
SsiI CCGC 9 cut(s) 23, 124, 180, 213, 216, 344, 347, 461, 594
SspMI CTAG 1 cut(s) 627
StyD4I CCNGG 5 cut(s) 186, 240, 380, 656, 928
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 3 cut(s) 38, 294, 342
TaqII GACCGA 1 cut(s) 572
TasI AATT 2 cut(s) 533, 849
TauI GCSGC 4 cut(s) 26, 216, 347, 350
TfiI GAWTC 4 cut(s) 159, 303, 433, 673
TscAI CASTG 3 cut(s) 45, 318, 716
TseFI GTSAC 2 cut(s) 36, 52
TseI GCWGC 3 cut(s) 438, 943, 946
Tsp45I GTSAC 2 cut(s) 36, 52
TspGWI ACGGA 2 cut(s) 71, 247
TspRI CASTG 3 cut(s) 45, 318, 716
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 555, 868
XapI RAATTY 2 cut(s) 533, 849
XceI RCATGY 1 cut(s) 943
XspI CTAG 1 cut(s) 627
ZraI GACGTC 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.